Lentinus tigrinus ALCF2SS1-6

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporaceae; Lentinus; Lentinus tigrinus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C2SLF8|A0A5C2SLF8_9APHY Acid protease OS=Lentinus tigrinus ALCF2SS1-6 OX=1328759 GN=L227DRAFT_573495 PE=3 SV=1
MM1 pKa = 7.14RR2 pKa = 11.84TFRR5 pKa = 11.84NEE7 pKa = 3.68EE8 pKa = 3.75DD9 pKa = 3.96TQPHH13 pKa = 5.47IQNMITHH20 pKa = 7.36GGRR23 pKa = 11.84PVLICNQEE31 pKa = 3.68VGNAHH36 pKa = 7.86LYY38 pKa = 7.52TIPRR42 pKa = 11.84PPTPPSNFSDD52 pKa = 3.7VCFEE56 pKa = 5.4LYY58 pKa = 10.45VQGPSDD64 pKa = 3.88EE65 pKa = 5.48DD66 pKa = 3.67VTSDD70 pKa = 3.42EE71 pKa = 4.14VSIISDD77 pKa = 4.06LFYY80 pKa = 10.93SPPSQPVVDD89 pKa = 5.04HH90 pKa = 6.67GPARR94 pKa = 11.84PDD96 pKa = 3.42TVSLLSEE103 pKa = 4.2GTTTTLCTNTLSEE116 pKa = 4.38SDD118 pKa = 3.57TLRR121 pKa = 11.84SGEE124 pKa = 4.29SKK126 pKa = 10.8QEE128 pKa = 3.9VYY130 pKa = 10.89LLQGPDD136 pKa = 2.97QPYY139 pKa = 10.7NFDD142 pKa = 3.63NQEE145 pKa = 3.22IWTDD149 pKa = 3.66DD150 pKa = 3.69LSDD153 pKa = 4.02LVIEE157 pKa = 4.73TGDD160 pKa = 3.45HH161 pKa = 4.98QMVIGMQPQYY171 pKa = 11.07EE172 pKa = 4.31DD173 pKa = 4.91NEE175 pKa = 4.29EE176 pKa = 3.95DD177 pKa = 3.22WLLEE181 pKa = 3.54ILLNDD186 pKa = 3.81EE187 pKa = 4.62EE188 pKa = 5.4DD189 pKa = 5.37DD190 pKa = 4.06EE191 pKa = 5.93VEE193 pKa = 4.43DD194 pKa = 4.24

Molecular weight: 21.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C2SPF6|A0A5C2SPF6_9APHY DEAD-domain-containing protein OS=Lentinus tigrinus ALCF2SS1-6 OX=1328759 GN=L227DRAFT_540637 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 4.96MSQSARR8 pKa = 11.84GPRR11 pKa = 11.84TFPASMPSHH20 pKa = 6.93PPSPPAAVAKK30 pKa = 10.33AQIRR34 pKa = 11.84LPPRR38 pKa = 11.84PPLHH42 pKa = 7.0PARR45 pKa = 11.84ARR47 pKa = 11.84PRR49 pKa = 11.84PSRR52 pKa = 11.84PRR54 pKa = 11.84AQRR57 pKa = 11.84PRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84TLRR64 pKa = 11.84RR65 pKa = 11.84PPRR68 pKa = 11.84RR69 pKa = 11.84LAARR73 pKa = 11.84WLPRR77 pKa = 11.84APSTHH82 pKa = 7.42LYY84 pKa = 9.91RR85 pKa = 5.88

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15504

8

15512

6373336

49

5032

410.9

45.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.215 ± 0.017

1.355 ± 0.008

5.589 ± 0.013

5.771 ± 0.021

3.6 ± 0.012

6.506 ± 0.018

2.645 ± 0.008

4.405 ± 0.013

4.07 ± 0.019

9.207 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.007

3.012 ± 0.01

6.978 ± 0.022

3.601 ± 0.011

6.655 ± 0.02

8.51 ± 0.028

6.021 ± 0.014

6.623 ± 0.014

1.476 ± 0.008

2.673 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski