Staphylococcus sp. CAG:324

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; environmental samples

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1476 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6TL38|R6TL38_9STAP Ferric uptake regulator Fur family OS=Staphylococcus sp. CAG:324 OX=1262969 GN=BN609_01024 PE=3 SV=1
MM1 pKa = 7.89KK2 pKa = 9.29ITLCGSDD9 pKa = 3.35VVVGIKK15 pKa = 10.56GITIIKK21 pKa = 9.99DD22 pKa = 3.43EE23 pKa = 4.53NPDD26 pKa = 3.5GVSVLSIDD34 pKa = 3.49NEE36 pKa = 4.08NYY38 pKa = 10.38YY39 pKa = 10.06IEE41 pKa = 4.89IDD43 pKa = 3.43EE44 pKa = 4.44EE45 pKa = 4.9TNVGYY50 pKa = 11.2VMQKK54 pKa = 10.42LDD56 pKa = 3.57NEE58 pKa = 4.8TIVLGTCEE66 pKa = 3.86VSVIVGDD73 pKa = 3.8KK74 pKa = 10.78DD75 pKa = 3.85PEE77 pKa = 4.39VLCVSAEE84 pKa = 3.75NSMM87 pKa = 4.12

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6UCJ1|R6UCJ1_9STAP Uncharacterized protein OS=Staphylococcus sp. CAG:324 OX=1262969 GN=BN609_00003 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.37RR10 pKa = 11.84KK11 pKa = 9.68HH12 pKa = 6.01SKK14 pKa = 7.21THH16 pKa = 5.41GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.95VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.81GRR39 pKa = 11.84AVLSAA44 pKa = 3.96

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1476

0

1476

503392

29

4055

341.1

38.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.172 ± 0.067

1.207 ± 0.026

5.707 ± 0.057

6.919 ± 0.063

4.626 ± 0.052

5.597 ± 0.062

1.699 ± 0.029

10.059 ± 0.075

8.519 ± 0.079

9.315 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.031

6.509 ± 0.067

2.603 ± 0.032

2.964 ± 0.035

2.961 ± 0.044

6.046 ± 0.055

5.522 ± 0.067

6.225 ± 0.053

0.67 ± 0.023

5.22 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski