Amino acid dipepetide frequency for alpha proteobacterium Q-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.05AlaAla: 14.05 ± 0.192	1.003AlaCys: 1.003 ± 0.033	7.661AlaAsp: 7.661 ± 0.1	6.519AlaGlu: 6.519 ± 0.102	4.549AlaPhe: 4.549 ± 0.08	9.385AlaGly: 9.385 ± 0.122	2.654AlaHis: 2.654 ± 0.059	6.641AlaIle: 6.641 ± 0.105	3.868AlaLys: 3.868 ± 0.076	13.852AlaLeu: 13.852 ± 0.151	3.533AlaMet: 3.533 ± 0.066	2.757AlaAsn: 2.757 ± 0.064	5.274AlaPro: 5.274 ± 0.086	4.664AlaGln: 4.664 ± 0.063	8.468AlaArg: 8.468 ± 0.121	6.095AlaSer: 6.095 ± 0.082	4.553AlaThr: 4.553 ± 0.076	7.041AlaVal: 7.041 ± 0.103	1.138AlaTrp: 1.138 ± 0.042	2.467AlaTyr: 2.467 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.94CysAla: 0.94 ± 0.034	0.106CysCys: 0.106 ± 0.011	0.595CysAsp: 0.595 ± 0.027	0.383CysGlu: 0.383 ± 0.022	0.363CysPhe: 0.363 ± 0.02	0.862CysGly: 0.862 ± 0.032	0.251CysHis: 0.251 ± 0.019	0.366CysIle: 0.366 ± 0.019	0.192CysLys: 0.192 ± 0.016	0.834CysLeu: 0.834 ± 0.036	0.124CysMet: 0.124 ± 0.013	0.203CysAsn: 0.203 ± 0.015	0.444CysPro: 0.444 ± 0.022	0.255CysGln: 0.255 ± 0.017	0.54CysArg: 0.54 ± 0.025	0.399CysSer: 0.399 ± 0.023	0.372CysThr: 0.372 ± 0.022	0.474CysVal: 0.474 ± 0.025	0.098CysTrp: 0.098 ± 0.011	0.18CysTyr: 0.18 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.913AspAla: 6.913 ± 0.098	0.499AspCys: 0.499 ± 0.024	4.446AspAsp: 4.446 ± 0.09	4.213AspGlu: 4.213 ± 0.063	2.814AspPhe: 2.814 ± 0.062	5.633AspGly: 5.633 ± 0.085	2.133AspHis: 2.133 ± 0.051	3.537AspIle: 3.537 ± 0.055	1.817AspLys: 1.817 ± 0.046	7.742AspLeu: 7.742 ± 0.095	1.556AspMet: 1.556 ± 0.038	1.42AspAsn: 1.42 ± 0.049	3.742AspPro: 3.742 ± 0.068	3.321AspGln: 3.321 ± 0.062	4.938AspArg: 4.938 ± 0.079	2.534AspSer: 2.534 ± 0.056	1.888AspThr: 1.888 ± 0.047	4.106AspVal: 4.106 ± 0.065	0.971AspTrp: 0.971 ± 0.035	1.739AspTyr: 1.739 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	7.017GluAla: 7.017 ± 0.097	0.309GluCys: 0.309 ± 0.018	3.341GluAsp: 3.341 ± 0.069	2.895GluGlu: 2.895 ± 0.061	1.505GluPhe: 1.505 ± 0.049	4.291GluGly: 4.291 ± 0.066	1.005GluHis: 1.005 ± 0.038	3.713GluIle: 3.713 ± 0.063	2.925GluLys: 2.925 ± 0.059	4.396GluLeu: 4.396 ± 0.082	1.552GluMet: 1.552 ± 0.039	1.815GluAsn: 1.815 ± 0.047	2.383GluPro: 2.383 ± 0.058	2.516GluGln: 2.516 ± 0.054	4.477GluArg: 4.477 ± 0.091	2.914GluSer: 2.914 ± 0.057	3.332GluThr: 3.332 ± 0.066	2.883GluVal: 2.883 ± 0.06	0.563GluTrp: 0.563 ± 0.023	0.8GluTyr: 0.8 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.275PheAla: 4.275 ± 0.074	0.397PheCys: 0.397 ± 0.021	2.904PheAsp: 2.904 ± 0.06	2.166PheGlu: 2.166 ± 0.044	1.697PhePhe: 1.697 ± 0.044	3.65PheGly: 3.65 ± 0.074	0.87PheHis: 0.87 ± 0.035	2.257PheIle: 2.257 ± 0.046	1.206PheLys: 1.206 ± 0.035	3.914PheLeu: 3.914 ± 0.075	0.96PheMet: 0.96 ± 0.03	1.226PheAsn: 1.226 ± 0.042	1.615PhePro: 1.615 ± 0.045	1.129PheGln: 1.129 ± 0.035	2.093PheArg: 2.093 ± 0.053	2.809PheSer: 2.809 ± 0.056	1.984PheThr: 1.984 ± 0.049	2.402PheVal: 2.402 ± 0.051	0.539PheTrp: 0.539 ± 0.028	1.086PheTyr: 1.086 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	8.579GlyAla: 8.579 ± 0.1	0.766GlyCys: 0.766 ± 0.03	5.042GlyAsp: 5.042 ± 0.075	4.294GlyGlu: 4.294 ± 0.07	3.824GlyPhe: 3.824 ± 0.075	6.997GlyGly: 6.997 ± 0.105	2.068GlyHis: 2.068 ± 0.053	4.47GlyIle: 4.47 ± 0.081	2.89GlyLys: 2.89 ± 0.058	9.414GlyLeu: 9.414 ± 0.111	2.178GlyMet: 2.178 ± 0.059	2.11GlyAsn: 2.11 ± 0.063	3.595GlyPro: 3.595 ± 0.062	3.144GlyGln: 3.144 ± 0.063	5.865GlyArg: 5.865 ± 0.092	4.588GlySer: 4.588 ± 0.083	3.973GlyThr: 3.973 ± 0.081	5.342GlyVal: 5.342 ± 0.083	1.237GlyTrp: 1.237 ± 0.039	2.128GlyTyr: 2.128 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.339HisAla: 2.339 ± 0.054	0.25HisCys: 0.25 ± 0.017	1.579HisAsp: 1.579 ± 0.044	1.313HisGlu: 1.313 ± 0.04	0.954HisPhe: 0.954 ± 0.036	1.97HisGly: 1.97 ± 0.055	0.803HisHis: 0.803 ± 0.035	1.254HisIle: 1.254 ± 0.035	0.792HisLys: 0.792 ± 0.027	2.63HisLeu: 2.63 ± 0.061	0.561HisMet: 0.561 ± 0.024	0.509HisAsn: 0.509 ± 0.023	1.449HisPro: 1.449 ± 0.043	0.972HisGln: 0.972 ± 0.036	1.584HisArg: 1.584 ± 0.048	1.147HisSer: 1.147 ± 0.032	0.57HisThr: 0.57 ± 0.024	1.444HisVal: 1.444 ± 0.045	0.372HisTrp: 0.372 ± 0.02	0.763HisTyr: 0.763 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	7.206IleAla: 7.206 ± 0.099	0.516IleCys: 0.516 ± 0.027	4.445IleAsp: 4.445 ± 0.072	3.956IleGlu: 3.956 ± 0.067	2.129IlePhe: 2.129 ± 0.053	5.065IleGly: 5.065 ± 0.083	1.135IleHis: 1.135 ± 0.039	3.541IleIle: 3.541 ± 0.072	2.148IleLys: 2.148 ± 0.053	5.688IleLeu: 5.688 ± 0.085	1.299IleMet: 1.299 ± 0.039	1.947IleAsn: 1.947 ± 0.047	2.503IlePro: 2.503 ± 0.056	1.23IleGln: 1.23 ± 0.036	3.509IleArg: 3.509 ± 0.063	3.95IleSer: 3.95 ± 0.068	3.284IleThr: 3.284 ± 0.064	3.266IleVal: 3.266 ± 0.071	0.694IleTrp: 0.694 ± 0.03	1.225IleTyr: 1.225 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	5.376LysAla: 5.376 ± 0.09	0.148LysCys: 0.148 ± 0.014	2.216LysAsp: 2.216 ± 0.057	1.607LysGlu: 1.607 ± 0.046	0.802LysPhe: 0.802 ± 0.036	3.012LysGly: 3.012 ± 0.059	0.614LysHis: 0.614 ± 0.026	2.226LysIle: 2.226 ± 0.05	1.675LysLys: 1.675 ± 0.059	3.093LysLeu: 3.093 ± 0.07	0.892LysMet: 0.892 ± 0.035	1.217LysAsn: 1.217 ± 0.04	2.187LysPro: 2.187 ± 0.057	1.108LysGln: 1.108 ± 0.032	2.458LysArg: 2.458 ± 0.056	2.442LysSer: 2.442 ± 0.055	2.101LysThr: 2.101 ± 0.052	1.819LysVal: 1.819 ± 0.046	0.309LysTrp: 0.309 ± 0.017	0.546LysTyr: 0.546 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	13.219LeuAla: 13.219 ± 0.159	1.012LeuCys: 1.012 ± 0.036	7.365LeuAsp: 7.365 ± 0.094	5.603LeuGlu: 5.603 ± 0.078	4.132LeuPhe: 4.132 ± 0.083	8.498LeuGly: 8.498 ± 0.098	2.045LeuHis: 2.045 ± 0.052	6.289LeuIle: 6.289 ± 0.092	4.169LeuLys: 4.169 ± 0.079	10.263LeuLeu: 10.263 ± 0.133	2.883LeuMet: 2.883 ± 0.061	2.835LeuAsn: 2.835 ± 0.06	5.512LeuPro: 5.512 ± 0.086	3.098LeuGln: 3.098 ± 0.054	6.486LeuArg: 6.486 ± 0.09	7.672LeuSer: 7.672 ± 0.105	4.543LeuThr: 4.543 ± 0.074	6.741LeuVal: 6.741 ± 0.105	1.362LeuTrp: 1.362 ± 0.044	2.483LeuTyr: 2.483 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	3.841MetAla: 3.841 ± 0.076	0.139MetCys: 0.139 ± 0.014	1.391MetAsp: 1.391 ± 0.035	1.234MetGlu: 1.234 ± 0.038	0.62MetPhe: 0.62 ± 0.027	2.304MetGly: 2.304 ± 0.051	0.492MetHis: 0.492 ± 0.028	1.787MetIle: 1.787 ± 0.049	1.087MetLys: 1.087 ± 0.034	2.447MetLeu: 2.447 ± 0.058	0.786MetMet: 0.786 ± 0.028	0.751MetAsn: 0.751 ± 0.033	1.502MetPro: 1.502 ± 0.041	0.811MetGln: 0.811 ± 0.031	1.882MetArg: 1.882 ± 0.046	1.557MetSer: 1.557 ± 0.045	1.615MetThr: 1.615 ± 0.04	1.661MetVal: 1.661 ± 0.049	0.185MetTrp: 0.185 ± 0.015	0.248MetTyr: 0.248 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.98AsnAla: 2.98 ± 0.057	0.239AsnCys: 0.239 ± 0.017	1.646AsnAsp: 1.646 ± 0.046	1.494AsnGlu: 1.494 ± 0.045	1.092AsnPhe: 1.092 ± 0.046	2.4AsnGly: 2.4 ± 0.06	0.572AsnHis: 0.572 ± 0.023	1.647AsnIle: 1.647 ± 0.047	0.896AsnLys: 0.896 ± 0.038	2.792AsnLeu: 2.792 ± 0.063	0.623AsnMet: 0.623 ± 0.026	0.773AsnAsn: 0.773 ± 0.035	1.98AsnPro: 1.98 ± 0.047	0.974AsnGln: 0.974 ± 0.034	2.031AsnArg: 2.031 ± 0.045	1.531AsnSer: 1.531 ± 0.045	1.09AsnThr: 1.09 ± 0.038	1.573AsnVal: 1.573 ± 0.045	0.396AsnTrp: 0.396 ± 0.019	0.622AsnTyr: 0.622 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.496ProAla: 5.496 ± 0.094	0.357ProCys: 0.357 ± 0.02	4.381ProAsp: 4.381 ± 0.067	3.239ProGlu: 3.239 ± 0.071	2.186ProPhe: 2.186 ± 0.046	4.075ProGly: 4.075 ± 0.075	1.296ProHis: 1.296 ± 0.038	2.627ProIle: 2.627 ± 0.055	1.867ProLys: 1.867 ± 0.052	5.191ProLeu: 5.191 ± 0.09	1.334ProMet: 1.334 ± 0.04	1.338ProAsn: 1.338 ± 0.042	2.459ProPro: 2.459 ± 0.061	2.03ProGln: 2.03 ± 0.048	2.732ProArg: 2.732 ± 0.066	2.821ProSer: 2.821 ± 0.055	2.013ProThr: 2.013 ± 0.047	3.6ProVal: 3.6 ± 0.06	0.624ProTrp: 0.624 ± 0.03	1.149ProTyr: 1.149 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.384GlnAla: 4.384 ± 0.081	0.273GlnCys: 0.273 ± 0.019	1.823GlnAsp: 1.823 ± 0.044	1.602GlnGlu: 1.602 ± 0.045	1.221GlnPhe: 1.221 ± 0.04	2.506GlnGly: 2.506 ± 0.053	0.734GlnHis: 0.734 ± 0.029	2.626GlnIle: 2.626 ± 0.052	1.819GlnLys: 1.819 ± 0.048	2.977GlnLeu: 2.977 ± 0.063	1.213GlnMet: 1.213 ± 0.033	1.272GlnAsn: 1.272 ± 0.035	1.998GlnPro: 1.998 ± 0.045	1.557GlnGln: 1.557 ± 0.054	2.429GlnArg: 2.429 ± 0.053	3.023GlnSer: 3.023 ± 0.059	1.93GlnThr: 1.93 ± 0.05	2.076GlnVal: 2.076 ± 0.053	0.481GlnTrp: 0.481 ± 0.024	0.578GlnTyr: 0.578 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	7.619ArgAla: 7.619 ± 0.1	0.478ArgCys: 0.478 ± 0.022	4.328ArgAsp: 4.328 ± 0.069	3.34ArgGlu: 3.34 ± 0.069	3.14ArgPhe: 3.14 ± 0.065	4.526ArgGly: 4.526 ± 0.076	1.996ArgHis: 1.996 ± 0.05	4.185ArgIle: 4.185 ± 0.061	2.231ArgLys: 2.231 ± 0.055	8.675ArgLeu: 8.675 ± 0.117	1.871ArgMet: 1.871 ± 0.041	1.713ArgAsn: 1.713 ± 0.049	3.451ArgPro: 3.451 ± 0.082	2.717ArgGln: 2.717 ± 0.058	4.909ArgArg: 4.909 ± 0.095	3.749ArgSer: 3.749 ± 0.074	2.988ArgThr: 2.988 ± 0.054	3.915ArgVal: 3.915 ± 0.074	0.862ArgTrp: 0.862 ± 0.028	1.677ArgTyr: 1.677 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.391SerAla: 6.391 ± 0.086	0.429SerCys: 0.429 ± 0.021	3.707SerAsp: 3.707 ± 0.069	2.761SerGlu: 2.761 ± 0.06	2.726SerPhe: 2.726 ± 0.057	5.646SerGly: 5.646 ± 0.09	1.366SerHis: 1.366 ± 0.038	3.234SerIle: 3.234 ± 0.065	1.751SerLys: 1.751 ± 0.05	6.964SerLeu: 6.964 ± 0.101	1.454SerMet: 1.454 ± 0.042	1.476SerAsn: 1.476 ± 0.043	3.07SerPro: 3.07 ± 0.061	2.006SerGln: 2.006 ± 0.049	3.907SerArg: 3.907 ± 0.067	3.09SerSer: 3.09 ± 0.066	2.619SerThr: 2.619 ± 0.052	3.827SerVal: 3.827 ± 0.065	0.713SerTrp: 0.713 ± 0.03	1.419SerTyr: 1.419 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	5.319ThrAla: 5.319 ± 0.081	0.32ThrCys: 0.32 ± 0.019	2.871ThrAsp: 2.871 ± 0.06	2.343ThrGlu: 2.343 ± 0.052	1.502ThrPhe: 1.502 ± 0.049	4.382ThrGly: 4.382 ± 0.072	0.973ThrHis: 0.973 ± 0.032	2.728ThrIle: 2.728 ± 0.058	1.414ThrLys: 1.414 ± 0.038	5.039ThrLeu: 5.039 ± 0.075	0.994ThrMet: 0.994 ± 0.034	1.262ThrAsn: 1.262 ± 0.043	2.896ThrPro: 2.896 ± 0.057	1.434ThrGln: 1.434 ± 0.043	2.859ThrArg: 2.859 ± 0.059	2.59ThrSer: 2.59 ± 0.063	2.14ThrThr: 2.14 ± 0.051	3.11ThrVal: 3.11 ± 0.067	0.46ThrTrp: 0.46 ± 0.023	0.956ThrTyr: 0.956 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	6.707ValAla: 6.707 ± 0.091	0.417ValCys: 0.417 ± 0.022	4.024ValAsp: 4.024 ± 0.075	3.733ValGlu: 3.733 ± 0.076	2.57ValPhe: 2.57 ± 0.048	4.382ValGly: 4.382 ± 0.072	1.309ValHis: 1.309 ± 0.039	4.016ValIle: 4.016 ± 0.081	2.072ValLys: 2.072 ± 0.052	6.338ValLeu: 6.338 ± 0.091	1.759ValMet: 1.759 ± 0.049	1.824ValAsn: 1.824 ± 0.045	3.111ValPro: 3.111 ± 0.063	1.838ValGln: 1.838 ± 0.051	4.386ValArg: 4.386 ± 0.079	3.7ValSer: 3.7 ± 0.073	3.175ValThr: 3.175 ± 0.064	4.29ValVal: 4.29 ± 0.082	0.595ValTrp: 0.595 ± 0.024	1.372ValTyr: 1.372 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.157TrpAla: 1.157 ± 0.036	0.099TrpCys: 0.099 ± 0.009	0.606TrpAsp: 0.606 ± 0.029	0.484TrpGlu: 0.484 ± 0.025	0.457TrpPhe: 0.457 ± 0.023	0.785TrpGly: 0.785 ± 0.035	0.33TrpHis: 0.33 ± 0.019	0.619TrpIle: 0.619 ± 0.025	0.36TrpLys: 0.36 ± 0.02	1.633TrpLeu: 1.633 ± 0.043	0.345TrpMet: 0.345 ± 0.022	0.315TrpAsn: 0.315 ± 0.018	0.643TrpPro: 0.643 ± 0.031	0.7TrpGln: 0.7 ± 0.029	1.031TrpArg: 1.031 ± 0.033	0.733TrpSer: 0.733 ± 0.024	0.61TrpThr: 0.61 ± 0.028	0.727TrpVal: 0.727 ± 0.032	0.182TrpTrp: 0.182 ± 0.013	0.254TrpTyr: 0.254 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.364TyrAla: 2.364 ± 0.056	0.223TyrCys: 0.223 ± 0.016	1.591TyrAsp: 1.591 ± 0.043	1.453TyrGlu: 1.453 ± 0.034	0.898TyrPhe: 0.898 ± 0.033	2.137TyrGly: 2.137 ± 0.05	0.606TyrHis: 0.606 ± 0.026	0.974TyrIle: 0.974 ± 0.035	0.67TyrLys: 0.67 ± 0.028	2.33TyrLeu: 2.33 ± 0.05	0.425TyrMet: 0.425 ± 0.024	0.585TyrAsn: 0.585 ± 0.027	1.001TyrPro: 1.001 ± 0.031	1.014TyrGln: 1.014 ± 0.036	1.787TyrArg: 1.787 ± 0.046	1.213TyrSer: 1.213 ± 0.036	0.81TyrThr: 0.81 ± 0.029	1.322TyrVal: 1.322 ± 0.046	0.289TyrTrp: 0.289 ± 0.017	0.591TyrTyr: 0.591 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2787 proteins (927933 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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