Cellulophaga tyrosinoxydans
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W2CPN8|A0A1W2CPN8_9FLAO DUF1080 domain-containing protein OS=Cellulophaga tyrosinoxydans OX=504486 GN=SAMN05660703_3116 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.39 LSNLIYY8 pKa = 10.4 AFSYY12 pKa = 10.39 LVAISFFASCNNDD25 pKa = 3.5 DD26 pKa = 5.69 DD27 pKa = 6.38 NDD29 pKa = 3.95 TTMMPDD35 pKa = 3.15 MNADD39 pKa = 3.88 FSGTYY44 pKa = 10.14 AQVDD48 pKa = 3.28 HH49 pKa = 6.84 MGRR52 pKa = 11.84 PGINTVLSADD62 pKa = 3.68 NATKK66 pKa = 10.84 DD67 pKa = 3.6 MQNITLPSNMAAAFQASFEE86 pKa = 4.24 ARR88 pKa = 11.84 LEE90 pKa = 4.36 AYY92 pKa = 9.99 HH93 pKa = 7.19 DD94 pKa = 3.93 VYY96 pKa = 11.81 ANILGADD103 pKa = 3.93 PAAVNYY109 pKa = 8.37 EE110 pKa = 3.92 NNILGLTAAQLTGYY124 pKa = 9.89 LAADD128 pKa = 3.97 VLDD131 pKa = 3.96 VAPNLPTTYY140 pKa = 10.18 FNPGTDD146 pKa = 2.92 ADD148 pKa = 3.67 MDD150 pKa = 3.93 GRR152 pKa = 11.84 ILVPDD157 pKa = 4.09 GDD159 pKa = 4.2 EE160 pKa = 4.01 VALTGRR166 pKa = 11.84 MLQDD170 pKa = 3.46 DD171 pKa = 5.09 VIDD174 pKa = 3.67 VSLILLFGGMEE185 pKa = 4.25 GNRR188 pKa = 11.84 FSGQDD193 pKa = 3.34 TNGDD197 pKa = 3.38 GMADD201 pKa = 4.24 LPRR204 pKa = 11.84 LTSDD208 pKa = 3.64 GVSITANPTTTFPYY222 pKa = 10.2 LANPEE227 pKa = 4.06
Molecular weight: 24.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.401
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.465
Grimsley 3.312
Solomon 3.643
Lehninger 3.605
Nozaki 3.783
DTASelect 4.088
Thurlkill 3.478
EMBOSS 3.643
Sillero 3.77
Patrickios 1.036
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A1W1Z5S4|A0A1W1Z5S4_9FLAO Uncharacterized protein OS=Cellulophaga tyrosinoxydans OX=504486 GN=SAMN05660703_1196 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.21 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.04 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.5 KK27 pKa = 10.78 KK28 pKa = 9.37 KK29 pKa = 10.83
Molecular weight: 3.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3140
0
3140
1080595
29
4873
344.1
38.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.607 ± 0.038
0.729 ± 0.014
5.519 ± 0.034
6.476 ± 0.045
5.228 ± 0.031
6.404 ± 0.048
1.669 ± 0.022
8.12 ± 0.041
7.903 ± 0.068
9.411 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.139 ± 0.026
6.37 ± 0.057
3.333 ± 0.03
3.258 ± 0.021
3.175 ± 0.025
6.474 ± 0.038
5.944 ± 0.065
6.148 ± 0.033
1.039 ± 0.02
4.053 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here