Streptomyces phage Bing

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bingvirus; Streptomyces virus Bing

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L1IW74|A0A2L1IW74_9CAUD Uncharacterized protein OS=Streptomyces phage Bing OX=2079427 GN=SEA_BING_38 PE=4 SV=1
MM1 pKa = 7.53TALTWDD7 pKa = 3.58KK8 pKa = 11.26AGEE11 pKa = 4.16RR12 pKa = 11.84YY13 pKa = 9.75YY14 pKa = 10.42EE15 pKa = 4.01TGVDD19 pKa = 3.65HH20 pKa = 6.89GVLYY24 pKa = 10.4IPNGSGVYY32 pKa = 10.27NEE34 pKa = 3.58GHH36 pKa = 6.49AWNGLTAVTEE46 pKa = 4.43SPSGAEE52 pKa = 4.33SNPQYY57 pKa = 11.32ADD59 pKa = 3.31NIKK62 pKa = 10.35YY63 pKa = 10.59LNLVSAEE70 pKa = 4.09EE71 pKa = 3.98FGGTIEE77 pKa = 4.84AFTYY81 pKa = 9.5PLAWEE86 pKa = 4.21QCDD89 pKa = 3.9GSATPTPGVSFGQQGRR105 pKa = 11.84KK106 pKa = 9.55SFGLSYY112 pKa = 9.55RR113 pKa = 11.84TKK115 pKa = 10.7LGNDD119 pKa = 3.95LDD121 pKa = 4.55GQDD124 pKa = 4.02HH125 pKa = 7.09GYY127 pKa = 10.58KK128 pKa = 10.3LHH130 pKa = 6.48LVYY133 pKa = 10.45GALAAPSEE141 pKa = 4.28KK142 pKa = 10.51AYY144 pKa = 9.79ATVNDD149 pKa = 4.08SPEE152 pKa = 4.78AITFSWEE159 pKa = 3.78FTTTPVEE166 pKa = 4.17VGNIAGQDD174 pKa = 3.69YY175 pKa = 10.76KK176 pKa = 9.95PTASITIDD184 pKa = 3.47STKK187 pKa = 10.38VDD189 pKa = 3.73AGALSTLEE197 pKa = 3.79EE198 pKa = 4.22FLYY201 pKa = 9.16GTEE204 pKa = 4.23GTDD207 pKa = 3.53PSLPTPAEE215 pKa = 4.01VYY217 pKa = 10.53AIFSGTVLTVTPTEE231 pKa = 3.91PAYY234 pKa = 10.64DD235 pKa = 3.65GATNTITIPTVTGVTYY251 pKa = 10.82YY252 pKa = 10.19IDD254 pKa = 5.44DD255 pKa = 3.78VAQAPGPVVITEE267 pKa = 4.18NKK269 pKa = 9.31VVEE272 pKa = 4.1ARR274 pKa = 11.84PNAGYY279 pKa = 11.03KK280 pKa = 8.43FTQPSDD286 pKa = 3.72NDD288 pKa = 3.6WLIGDD293 pKa = 4.49FF294 pKa = 4.65

Molecular weight: 31.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L1IW79|A0A2L1IW79_9CAUD Endolysin OS=Streptomyces phage Bing OX=2079427 GN=SEA_BING_4 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 4.94RR3 pKa = 11.84PDD5 pKa = 3.26RR6 pKa = 11.84SGGSRR11 pKa = 11.84SLKK14 pKa = 10.06HH15 pKa = 5.82YY16 pKa = 9.56GIKK19 pKa = 9.61GMKK22 pKa = 7.98WGVRR26 pKa = 11.84RR27 pKa = 11.84SDD29 pKa = 3.25AQLANARR36 pKa = 11.84SAPKK40 pKa = 8.81PTLSEE45 pKa = 4.09DD46 pKa = 2.93ARR48 pKa = 11.84TAEE51 pKa = 3.96RR52 pKa = 11.84LHH54 pKa = 6.76NKK56 pKa = 8.7VQTKK60 pKa = 8.49GTGSLSNQEE69 pKa = 3.4MRR71 pKa = 11.84QFIEE75 pKa = 3.83RR76 pKa = 11.84MNLEE80 pKa = 4.03RR81 pKa = 11.84QYY83 pKa = 10.67SQLMSSPPGKK93 pKa = 9.79SAADD97 pKa = 3.71RR98 pKa = 11.84GHH100 pKa = 6.77DD101 pKa = 3.59QVKK104 pKa = 10.32KK105 pKa = 9.58YY106 pKa = 10.37LAYY109 pKa = 9.62GQTYY113 pKa = 9.8ANVRR117 pKa = 11.84KK118 pKa = 9.4FLDD121 pKa = 3.77TPAGQSIKK129 pKa = 10.13TGMKK133 pKa = 9.23AAAAAGFGYY142 pKa = 9.03ATGGTGPAAAAGAGVLVRR160 pKa = 11.84RR161 pKa = 11.84MTQQ164 pKa = 2.53

Molecular weight: 17.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

17442

38

1822

200.5

22.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.397 ± 0.513

0.459 ± 0.095

6.158 ± 0.288

7.132 ± 0.423

3.681 ± 0.217

7.597 ± 0.505

1.743 ± 0.24

5.292 ± 0.2

6.708 ± 0.381

7.837 ± 0.443

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.132

4.053 ± 0.131

4.002 ± 0.251

3.325 ± 0.142

5.114 ± 0.363

5.917 ± 0.22

6.691 ± 0.326

7.281 ± 0.18

1.491 ± 0.154

3.457 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski