Salmonella phage Segz_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BAN8|A0A411BAN8_9CAUD Putative tail assembly protein OS=Salmonella phage Segz_1 OX=2419756 GN=Segz_49 PE=3 SV=1
MM1 pKa = 6.99SQWIKK6 pKa = 10.51CSEE9 pKa = 3.86RR10 pKa = 11.84MPEE13 pKa = 3.91HH14 pKa = 6.49FSDD17 pKa = 3.99VLVTDD22 pKa = 4.63GVGVEE27 pKa = 4.32VKK29 pKa = 9.86WLDD32 pKa = 4.33EE33 pKa = 4.25NYY35 pKa = 10.08CWDD38 pKa = 3.99SFTEE42 pKa = 4.01NNSNIRR48 pKa = 11.84SCDD51 pKa = 3.23VVYY54 pKa = 10.39WMPLPEE60 pKa = 4.99PPQEE64 pKa = 3.96

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BAU8|A0A411BAU8_9CAUD Uncharacterized protein OS=Salmonella phage Segz_1 OX=2419756 GN=Segz_69 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.78TIYY6 pKa = 9.92RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.95RR10 pKa = 11.84LEE12 pKa = 4.17SEE14 pKa = 4.18LGHH17 pKa = 7.12VGAQNFMSKK26 pKa = 10.15HH27 pKa = 5.35ARR29 pKa = 11.84NAMEE33 pKa = 4.61SIRR36 pKa = 11.84VNRR39 pKa = 11.84VVRR42 pKa = 11.84VFNGEE47 pKa = 3.69GKK49 pKa = 10.08RR50 pKa = 11.84RR51 pKa = 11.84VMDD54 pKa = 3.54EE55 pKa = 4.36LIIVFF60 pKa = 4.5

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14317

32

1097

190.9

21.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.926 ± 0.756

1.425 ± 0.198

6.356 ± 0.353

6.601 ± 0.269

3.436 ± 0.209

7.229 ± 0.26

1.83 ± 0.209

6.517 ± 0.232

6.391 ± 0.369

7.11 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.157 ± 0.188

4.68 ± 0.232

3.269 ± 0.276

4.037 ± 0.402

5.42 ± 0.269

6.426 ± 0.27

5.707 ± 0.391

6.405 ± 0.241

1.495 ± 0.134

3.583 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski