Streptococcus phage Javan64

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BDY3|A0A4D6BDY3_9CAUD Lysozyme OS=Streptococcus phage Javan64 OX=2548295 GN=Javan64_0007 PE=3 SV=1
MM1 pKa = 7.9DD2 pKa = 4.89AAEE5 pKa = 4.96IYY7 pKa = 10.79DD8 pKa = 4.04NYY10 pKa = 10.29IQIDD14 pKa = 3.7NFEE17 pKa = 4.62DD18 pKa = 4.23FSYY21 pKa = 10.18WFEE24 pKa = 3.8QQIYY28 pKa = 10.19YY29 pKa = 8.45LTRR32 pKa = 11.84DD33 pKa = 3.41QFRR36 pKa = 11.84KK37 pKa = 9.51IDD39 pKa = 4.48GIWLKK44 pKa = 10.82MMIDD48 pKa = 4.24CYY50 pKa = 10.93KK51 pKa = 10.7KK52 pKa = 10.13RR53 pKa = 11.84DD54 pKa = 3.6EE55 pKa = 4.83LLFF58 pKa = 4.95

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BHP7|A0A4D6BHP7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan64 OX=2548295 GN=Javan64_0036 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.08IGMRR6 pKa = 11.84TPSLKK11 pKa = 10.29RR12 pKa = 11.84SLKK15 pKa = 10.71ARR17 pKa = 11.84TTSKK21 pKa = 9.91WKK23 pKa = 10.1RR24 pKa = 11.84QIKK27 pKa = 9.7KK28 pKa = 10.4AVIPGYY34 pKa = 7.47GQKK37 pKa = 10.88GIGWIEE43 pKa = 3.9NPKK46 pKa = 9.79KK47 pKa = 10.73AMYY50 pKa = 10.08NKK52 pKa = 9.65VYY54 pKa = 10.76RR55 pKa = 11.84KK56 pKa = 6.5TTFGLSDD63 pKa = 3.07IVKK66 pKa = 9.79SSKK69 pKa = 10.07EE70 pKa = 3.67KK71 pKa = 10.86SSAKK75 pKa = 9.66VKK77 pKa = 10.54KK78 pKa = 10.13KK79 pKa = 10.58AIRR82 pKa = 11.84QSKK85 pKa = 10.45DD86 pKa = 3.05YY87 pKa = 9.34TAKK90 pKa = 10.51DD91 pKa = 3.54YY92 pKa = 10.82KK93 pKa = 10.47QAGIVMIILGLLLMFVIPVLGIFFLILGIISFGVATLFSKK133 pKa = 10.49KK134 pKa = 9.12YY135 pKa = 9.86SRR137 pKa = 11.84SKK139 pKa = 10.93

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11835

37

1354

211.3

23.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.224 ± 0.674

0.575 ± 0.103

5.83 ± 0.317

7.114 ± 0.408

4.292 ± 0.287

6.54 ± 0.494

1.377 ± 0.115

6.895 ± 0.303

8.813 ± 0.461

8.179 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.161

6.041 ± 0.369

2.822 ± 0.222

4.199 ± 0.252

4.402 ± 0.289

6.227 ± 0.314

5.982 ± 0.344

5.94 ± 0.243

1.31 ± 0.102

3.853 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski