Capybara microvirus Cap1_SP_93
Average proteome isoelectric point is 5.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W424|A0A4P8W424_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_93 OX=2584799 PE=4 SV=1
MM1 pKa = 7.72 AFFPLNFFGASICEE15 pKa = 3.88 FVPTEE20 pKa = 3.76 KK21 pKa = 10.33 RR22 pKa = 11.84 YY23 pKa = 9.94 ILSKK27 pKa = 10.99 SIDD30 pKa = 3.68 PNNEE34 pKa = 3.67 NNVLTSGVLDD44 pKa = 3.91 DD45 pKa = 5.12 VPIFEE50 pKa = 4.93 NSKK53 pKa = 10.63 DD54 pKa = 4.0 LPNPDD59 pKa = 3.76 DD60 pKa = 3.57 TDD62 pKa = 3.71 LARR65 pKa = 11.84 QLLLGSPIQDD75 pKa = 3.29 YY76 pKa = 11.39 SGTKK80 pKa = 9.26 ILNGKK85 pKa = 8.12 EE86 pKa = 3.98 LVISDD91 pKa = 4.04 SQANDD96 pKa = 3.22 YY97 pKa = 10.61 LAEE100 pKa = 4.18 LEE102 pKa = 4.21 KK103 pKa = 10.57 TLVKK107 pKa = 10.82 NN108 pKa = 3.91
Molecular weight: 12.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.404
IPC2_protein 4.24
IPC_protein 4.151
Toseland 3.961
ProMoST 4.304
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.05
Rodwell 3.986
Grimsley 3.872
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.457
Thurlkill 3.999
EMBOSS 4.062
Sillero 4.266
Patrickios 3.897
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.187
Protein with the highest isoelectric point:
>tr|A0A4V1FVN5|A0A4V1FVN5_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_93 OX=2584799 PE=4 SV=1
MM1 pKa = 7.49 YY2 pKa = 11.11 NMSTCYY8 pKa = 10.73 NKK10 pKa = 9.97 IEE12 pKa = 4.13 IKK14 pKa = 10.62 NPSVHH19 pKa = 5.89 FRR21 pKa = 11.84 KK22 pKa = 10.51 NMDD25 pKa = 3.52 KK26 pKa = 11.09 LLLQVDD32 pKa = 4.65 CGRR35 pKa = 11.84 CDD37 pKa = 2.92 SCLSQKK43 pKa = 10.39 RR44 pKa = 11.84 NNWYY48 pKa = 9.36 VRR50 pKa = 11.84 ARR52 pKa = 11.84 FEE54 pKa = 5.31 LMACLGQDD62 pKa = 3.0 KK63 pKa = 10.17 DD64 pKa = 3.64 GHH66 pKa = 5.66 YY67 pKa = 9.99 VAPSGFCLFPTLTYY81 pKa = 10.69 LSYY84 pKa = 10.85 KK85 pKa = 10.18 RR86 pKa = 11.84 PSLHH90 pKa = 7.37 IIDD93 pKa = 4.91 EE94 pKa = 4.86 FGDD97 pKa = 3.35 DD98 pKa = 3.35 WFFEE102 pKa = 4.45 GFSYY106 pKa = 9.9 QHH108 pKa = 5.62 ITDD111 pKa = 3.95 YY112 pKa = 11.65 VNTIQTQYY120 pKa = 9.22 KK121 pKa = 9.52 RR122 pKa = 11.84 KK123 pKa = 9.71 YY124 pKa = 8.88 NKK126 pKa = 9.27 PDD128 pKa = 3.49 DD129 pKa = 3.9 GVRR132 pKa = 11.84 YY133 pKa = 7.32 MVCSEE138 pKa = 3.97 YY139 pKa = 11.12 GGNAEE144 pKa = 4.15 YY145 pKa = 10.7 LDD147 pKa = 4.0 EE148 pKa = 5.7 RR149 pKa = 11.84 GLPRR153 pKa = 11.84 IAQAAPHH160 pKa = 4.89 YY161 pKa = 9.89 HH162 pKa = 6.65 CLLFFPARR170 pKa = 11.84 ASKK173 pKa = 10.9 DD174 pKa = 3.21 SLYY177 pKa = 10.49 WKK179 pKa = 10.19 DD180 pKa = 3.56 YY181 pKa = 10.96 VSNLWRR187 pKa = 11.84 KK188 pKa = 7.57 QHH190 pKa = 6.92 DD191 pKa = 3.87 NALVGYY197 pKa = 8.85 SKK199 pKa = 11.16 SKK201 pKa = 9.96 GAFVRR206 pKa = 11.84 DD207 pKa = 3.51 CDD209 pKa = 4.15 AIKK212 pKa = 10.81 YY213 pKa = 6.01 VTSYY217 pKa = 10.5 CHH219 pKa = 6.37 KK220 pKa = 10.64 QFDD223 pKa = 4.68 YY224 pKa = 10.73 YY225 pKa = 10.94 QRR227 pKa = 11.84 PEE229 pKa = 3.6 LDD231 pKa = 2.83 AYY233 pKa = 9.63 IWHH236 pKa = 7.84 DD237 pKa = 3.84 YY238 pKa = 10.56 IKK240 pKa = 10.87 DD241 pKa = 3.48 GFRR244 pKa = 11.84 FQIPPMSQWKK254 pKa = 10.32 DD255 pKa = 3.55 NEE257 pKa = 3.92 KK258 pKa = 10.99 LEE260 pKa = 4.0 YY261 pKa = 11.01 LEE263 pKa = 4.03 ILKK266 pKa = 10.4 SRR268 pKa = 11.84 RR269 pKa = 11.84 EE270 pKa = 3.98 EE271 pKa = 4.06 LKK273 pKa = 10.9 SIMPNHH279 pKa = 6.87 WEE281 pKa = 3.91 SGHH284 pKa = 6.55 FGEE287 pKa = 6.22 NCAKK291 pKa = 9.72 WLSNLSLDD299 pKa = 3.48 EE300 pKa = 4.11 RR301 pKa = 11.84 VNILSNGITFANDD314 pKa = 2.86 TNLEE318 pKa = 4.04 GKK320 pKa = 9.71 LNHH323 pKa = 6.02 YY324 pKa = 8.04 VVPKK328 pKa = 10.54 YY329 pKa = 10.62 IFDD332 pKa = 3.67 KK333 pKa = 10.8 LYY335 pKa = 11.15 YY336 pKa = 9.76 DD337 pKa = 3.29 IQYY340 pKa = 9.94 QRR342 pKa = 11.84 VYY344 pKa = 11.28 DD345 pKa = 3.93 EE346 pKa = 5.17 DD347 pKa = 4.57 LDD349 pKa = 4.93 RR350 pKa = 11.84 EE351 pKa = 4.48 VLRR354 pKa = 11.84 PALKK358 pKa = 10.4 KK359 pKa = 9.54 PTDD362 pKa = 3.65 ILCKK366 pKa = 10.6 DD367 pKa = 3.97 FVSLFRR373 pKa = 11.84 KK374 pKa = 9.81 VYY376 pKa = 10.17 NDD378 pKa = 3.28 KK379 pKa = 10.82 LLKK382 pKa = 10.19 FNHH385 pKa = 6.53 LFRR388 pKa = 11.84 YY389 pKa = 7.92 ITLCDD394 pKa = 3.51 LTDD397 pKa = 3.9 NEE399 pKa = 4.46 ILQLKK404 pKa = 10.07 NIDD407 pKa = 3.44 NKK409 pKa = 10.59 RR410 pKa = 11.84 DD411 pKa = 3.62 YY412 pKa = 10.58 VKK414 pKa = 10.73 NWTRR418 pKa = 11.84 EE419 pKa = 3.77 DD420 pKa = 3.68 LYY422 pKa = 11.41 SYY424 pKa = 11.18 FEE426 pKa = 4.73 DD427 pKa = 4.11 FQSRR431 pKa = 11.84 FSMEE435 pKa = 3.85 RR436 pKa = 11.84 LVSYY440 pKa = 10.37 SYY442 pKa = 10.92 IRR444 pKa = 11.84 NTLFDD449 pKa = 4.51 INTSLDD455 pKa = 3.85 LLQMSDD461 pKa = 3.75 EE462 pKa = 4.45 DD463 pKa = 4.4 FISNVEE469 pKa = 3.74 DD470 pKa = 3.19 HH471 pKa = 7.08 KK472 pKa = 11.29 IYY474 pKa = 11.03 VSLSNKK480 pKa = 9.8 SFSLKK485 pKa = 10.26 KK486 pKa = 10.38 GAEE489 pKa = 4.17 PYY491 pKa = 10.43 YY492 pKa = 10.94 KK493 pKa = 10.42 SDD495 pKa = 3.91 DD496 pKa = 3.7 YY497 pKa = 11.72 KK498 pKa = 11.45 HH499 pKa = 6.7 NIRR502 pKa = 11.84 NLIPYY507 pKa = 9.52 RR508 pKa = 11.84 EE509 pKa = 4.13 PEE511 pKa = 3.86 KK512 pKa = 11.04 EE513 pKa = 4.21 FILEE517 pKa = 3.83 LGEE520 pKa = 4.42 RR521 pKa = 11.84 INLIKK526 pKa = 10.76 SKK528 pKa = 10.72 NRR530 pKa = 11.84 LNAYY534 pKa = 7.42 HH535 pKa = 7.57 TIRR538 pKa = 11.84 KK539 pKa = 8.13 LQQQDD544 pKa = 3.5 KK545 pKa = 10.18 QNHH548 pKa = 6.51 LLTSLIVV555 pKa = 3.26
Molecular weight: 66.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.76
IPC2_protein 7.0
IPC_protein 6.942
Toseland 6.825
ProMoST 7.483
Dawson 7.585
Bjellqvist 7.819
Wikipedia 7.512
Rodwell 7.585
Grimsley 6.927
Solomon 7.614
Lehninger 7.629
Nozaki 7.995
DTASelect 7.761
Thurlkill 7.805
EMBOSS 7.834
Sillero 8.039
Patrickios 3.846
IPC_peptide 7.614
IPC2_peptide 7.161
IPC2.peptide.svr19 7.139
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1728
108
678
432.0
49.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.382 ± 1.37
1.505 ± 0.412
7.465 ± 0.481
4.514 ± 0.898
5.035 ± 0.954
5.208 ± 0.714
2.199 ± 0.691
5.845 ± 0.102
6.076 ± 1.015
8.507 ± 1.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.652
6.655 ± 0.379
3.993 ± 0.999
5.324 ± 1.201
4.282 ± 0.708
8.044 ± 0.674
5.324 ± 1.199
4.745 ± 0.252
1.62 ± 0.29
5.787 ± 0.976
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here