Prevotella copri CAG:164
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2331 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6BQZ2|R6BQZ2_9BACT Putative lipoprotein OS=Prevotella copri CAG:164 OX=1263102 GN=BN510_00163 PE=4 SV=1
MM1 pKa = 7.29 EE2 pKa = 4.34 QNKK5 pKa = 10.08 NNGTTSAAKK14 pKa = 9.26 WKK16 pKa = 9.42 EE17 pKa = 3.72 VLIGGVSGIALGTAGTLFASSIPLEE42 pKa = 4.5 ASAEE46 pKa = 4.25 TPEE49 pKa = 4.36 TPEE52 pKa = 4.82 SEE54 pKa = 4.29 DD55 pKa = 3.65 ANVGNGGEE63 pKa = 4.14 NVTAQMATCVTDD75 pKa = 3.53 SMSFSAAFGAARR87 pKa = 11.84 AEE89 pKa = 4.18 VGPGGVFEE97 pKa = 4.22 WHH99 pKa = 6.36 GNLYY103 pKa = 8.62 GTYY106 pKa = 9.4 TADD109 pKa = 3.14 EE110 pKa = 4.56 WNSMDD115 pKa = 3.34 SDD117 pKa = 3.49 ARR119 pKa = 11.84 NEE121 pKa = 4.14 YY122 pKa = 10.77 AEE124 pKa = 4.59 SIHH127 pKa = 6.58 WDD129 pKa = 4.11 GPSHH133 pKa = 6.75 EE134 pKa = 4.68 YY135 pKa = 10.29 VAASTSHH142 pKa = 6.28 SAHH145 pKa = 6.73 TYY147 pKa = 6.25 TANHH151 pKa = 5.9 SVADD155 pKa = 4.17 SVCQQQTPPEE165 pKa = 4.5 KK166 pKa = 10.3 DD167 pKa = 3.58 DD168 pKa = 4.34 EE169 pKa = 4.34 EE170 pKa = 4.73 TPDD173 pKa = 3.43 VEE175 pKa = 4.91 IIGVEE180 pKa = 3.8 HH181 pKa = 6.26 TNIDD185 pKa = 3.78 GEE187 pKa = 4.21 HH188 pKa = 7.04 DD189 pKa = 4.37 SIIGSASVNGQAVYY203 pKa = 9.95 FIDD206 pKa = 5.25 VDD208 pKa = 3.82 GQDD211 pKa = 3.72 NEE213 pKa = 4.42 FEE215 pKa = 5.22 LMVTDD220 pKa = 5.18 ANGNQQIDD228 pKa = 3.65 EE229 pKa = 4.67 GEE231 pKa = 4.23 VVDD234 pKa = 6.41 ISDD237 pKa = 3.17 QHH239 pKa = 5.88 MSVSHH244 pKa = 6.08 FEE246 pKa = 3.99 EE247 pKa = 4.38 MAQANHH253 pKa = 6.7 TSDD256 pKa = 3.83 TDD258 pKa = 3.71 NDD260 pKa = 4.0 DD261 pKa = 3.85 TPVQYY266 pKa = 10.67 YY267 pKa = 11.04 ANNEE271 pKa = 4.09 DD272 pKa = 4.02 LPDD275 pKa = 3.86 YY276 pKa = 11.6 VNDD279 pKa = 4.46 ADD281 pKa = 4.87 PGTLAA286 pKa = 4.79
Molecular weight: 30.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.884
Patrickios 0.655
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|R6BPP6|R6BPP6_9BACT ATP phosphoribosyltransferase OS=Prevotella copri CAG:164 OX=1263102 GN=hisG PE=3 SV=1
MM1 pKa = 7.85 PNGKK5 pKa = 9.19 KK6 pKa = 10.25 KK7 pKa = 10.12 KK8 pKa = 7.0 GHH10 pKa = 6.14 KK11 pKa = 9.06 MATHH15 pKa = 6.13 KK16 pKa = 10.39 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.25 NRR25 pKa = 11.84 HH26 pKa = 4.69 KK27 pKa = 11.1 SKK29 pKa = 11.1
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2331
0
2331
805592
29
2512
345.6
38.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.467 ± 0.046
1.31 ± 0.017
5.762 ± 0.034
6.432 ± 0.053
4.428 ± 0.035
6.905 ± 0.052
2.121 ± 0.025
6.743 ± 0.043
7.195 ± 0.042
8.779 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.089 ± 0.023
5.058 ± 0.037
3.501 ± 0.028
3.723 ± 0.028
4.299 ± 0.036
5.834 ± 0.043
5.367 ± 0.036
6.486 ± 0.039
1.257 ± 0.019
4.242 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here