Tilletiaria anomala (strain ATCC 24038 / CBS 436.72 / UBC 951)
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6806 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A066W2J0|A0A066W2J0_TILAU Uncharacterized protein OS=Tilletiaria anomala (strain ATCC 24038 / CBS 436.72 / UBC 951) OX=1037660 GN=K437DRAFT_87959 PE=4 SV=1
MM1 pKa = 7.33 FSVSACSEE9 pKa = 3.95 EE10 pKa = 5.75 DD11 pKa = 2.99 VDD13 pKa = 5.84 KK14 pKa = 11.25 PGNDD18 pKa = 3.03 SSAQDD23 pKa = 3.69 EE24 pKa = 4.62 LVQQHH29 pKa = 7.09 DD30 pKa = 3.94 EE31 pKa = 4.76 DD32 pKa = 6.63 DD33 pKa = 4.53 DD34 pKa = 4.66 DD35 pKa = 4.61 EE36 pKa = 5.3 CFEE39 pKa = 5.06 DD40 pKa = 4.55 SLAEE44 pKa = 3.85 RR45 pKa = 11.84 LVVQEE50 pKa = 4.36 MLDD53 pKa = 3.85 DD54 pKa = 4.83 LSCSSEE60 pKa = 4.22 SEE62 pKa = 3.62 IDD64 pKa = 3.78 VVEE67 pKa = 4.12 EE68 pKa = 5.62 DD69 pKa = 3.48 EE70 pKa = 6.21 DD71 pKa = 3.9 EE72 pKa = 4.3 DD73 pKa = 4.34 ARR75 pKa = 11.84 LHH77 pKa = 5.99 EE78 pKa = 4.62 VPADD82 pKa = 3.37 TMEE85 pKa = 4.38 PVEE88 pKa = 4.73 FPLTSRR94 pKa = 11.84 YY95 pKa = 9.82 SGYY98 pKa = 11.14 EE99 pKa = 3.77 NVEE102 pKa = 3.84 KK103 pKa = 11.15 VQDD106 pKa = 3.77 VKK108 pKa = 11.53 LDD110 pKa = 3.75 GEE112 pKa = 4.89 DD113 pKa = 3.9 YY114 pKa = 10.81 GHH116 pKa = 6.52 QDD118 pKa = 3.9 EE119 pKa = 5.56 IVADD123 pKa = 3.78 SDD125 pKa = 3.77 NVSRR129 pKa = 11.84 SSAAQGTCTVPMYY142 pKa = 9.83 PLSPSPSPLNPASDD156 pKa = 3.76 VLQIHH161 pKa = 6.41 MDD163 pKa = 3.76 SEE165 pKa = 4.79 FEE167 pKa = 4.05 LASYY171 pKa = 9.04 TGSASEE177 pKa = 4.17 ASSDD181 pKa = 4.13 EE182 pKa = 4.2 EE183 pKa = 4.59 FCTGAYY189 pKa = 9.37 LVHH192 pKa = 6.98
Molecular weight: 21.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 3.579
IPC_protein 3.579
Toseland 3.363
ProMoST 3.732
Dawson 3.567
Bjellqvist 3.719
Wikipedia 3.49
Rodwell 3.401
Grimsley 3.274
Solomon 3.554
Lehninger 3.516
Nozaki 3.681
DTASelect 3.884
Thurlkill 3.414
EMBOSS 3.503
Sillero 3.694
Patrickios 0.629
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.688
Protein with the highest isoelectric point:
>tr|A0A066VCG5|A0A066VCG5_TILAU UPF0172-domain-containing protein OS=Tilletiaria anomala (strain ATCC 24038 / CBS 436.72 / UBC 951) OX=1037660 GN=K437DRAFT_259173 PE=3 SV=1
MM1 pKa = 7.25 LRR3 pKa = 11.84 SATTALTLGPTKK15 pKa = 10.78 GSLTAMTHH23 pKa = 4.73 IRR25 pKa = 11.84 SRR27 pKa = 11.84 CPRR30 pKa = 11.84 RR31 pKa = 11.84 SAPSAALSPRR41 pKa = 11.84 LHH43 pKa = 6.13 HH44 pKa = 6.69 HH45 pKa = 6.25 PFRR48 pKa = 11.84 AKK50 pKa = 10.12 RR51 pKa = 11.84 HH52 pKa = 5.36 PHH54 pKa = 5.83 HH55 pKa = 6.78 QSQSSRR61 pKa = 11.84 PCMRR65 pKa = 11.84 RR66 pKa = 11.84 SQRR69 pKa = 11.84 SKK71 pKa = 10.82 GRR73 pKa = 11.84 SRR75 pKa = 11.84 WKK77 pKa = 8.56 TQTPTLMIMVITTAITRR94 pKa = 11.84 KK95 pKa = 7.59 TNGRR99 pKa = 11.84 NFVAHH104 pKa = 7.25 GGRR107 pKa = 11.84 PATRR111 pKa = 11.84 GKK113 pKa = 10.6
Molecular weight: 12.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.062
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.114
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6806
0
6806
3543731
49
6839
520.7
56.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.083 ± 0.035
1.162 ± 0.01
5.344 ± 0.021
5.706 ± 0.029
3.203 ± 0.018
7.46 ± 0.034
2.538 ± 0.013
4.089 ± 0.02
4.377 ± 0.023
8.888 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.024 ± 0.01
3.096 ± 0.015
5.948 ± 0.029
4.332 ± 0.02
6.503 ± 0.024
9.579 ± 0.046
5.535 ± 0.017
5.776 ± 0.021
1.197 ± 0.009
2.161 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here