Idiomarinaceae phage 1N2-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088FAW2|A0A088FAW2_9CAUD Putative tail fiber adhesion protein OS=Idiomarinaceae phage 1N2-2 OX=1536592 GN=N22_024 PE=4 SV=1
MM1 pKa = 7.81AGQGILGRR9 pKa = 11.84QVVYY13 pKa = 9.82TIGGQSILGINTKK26 pKa = 10.38GLTVNGEE33 pKa = 4.25PVDD36 pKa = 4.06VTDD39 pKa = 5.45DD40 pKa = 4.62NSDD43 pKa = 2.98GWQEE47 pKa = 3.97LLATPGVRR55 pKa = 11.84NAEE58 pKa = 3.92ITASLVVKK66 pKa = 10.21NLEE69 pKa = 3.83MLRR72 pKa = 11.84AIMTNSSQMYY82 pKa = 9.42AFTGTYY88 pKa = 9.73PDD90 pKa = 4.07GSSVAGNAFFANYY103 pKa = 10.31SEE105 pKa = 4.28TGEE108 pKa = 4.01TAEE111 pKa = 5.43AYY113 pKa = 7.9TADD116 pKa = 3.44VTFQLSGAPTFTAGSGGQGSS136 pKa = 3.57

Molecular weight: 14.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088F877|A0A088F877_9CAUD Putative major capsid protein OS=Idiomarinaceae phage 1N2-2 OX=1536592 GN=N22_010 PE=4 SV=1
MM1 pKa = 7.36GKK3 pKa = 9.73AAKK6 pKa = 10.05LKK8 pKa = 9.52EE9 pKa = 3.96LRR11 pKa = 11.84RR12 pKa = 11.84LARR15 pKa = 11.84RR16 pKa = 11.84TTKK19 pKa = 10.29HH20 pKa = 5.93LPEE23 pKa = 6.19HH24 pKa = 6.66EE25 pKa = 4.09LTANDD30 pKa = 3.45RR31 pKa = 11.84VKK33 pKa = 10.21TINTQGTQRR42 pKa = 11.84LGEE45 pKa = 4.35CTRR48 pKa = 11.84GAYY51 pKa = 9.03KK52 pKa = 10.46ALKK55 pKa = 8.4KK56 pKa = 10.69GKK58 pKa = 8.32VRR60 pKa = 11.84RR61 pKa = 11.84VEE63 pKa = 3.81

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11137

32

765

198.9

21.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.437 ± 0.468

1.095 ± 0.148

6.582 ± 0.275

6.384 ± 0.232

3.143 ± 0.198

8.306 ± 0.425

2.308 ± 0.253

5.962 ± 0.251

5.253 ± 0.451

7.507 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.231

4.597 ± 0.256

3.771 ± 0.239

4.184 ± 0.189

6.025 ± 0.232

6.285 ± 0.348

5.576 ± 0.282

6.528 ± 0.262

1.491 ± 0.127

2.828 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski