Tamlana nanhaiensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Tamlana

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D7W1C4|A0A0D7W1C4_9FLAO Mechanosensitive ion channel protein MscS OS=Tamlana nanhaiensis OX=1382798 GN=PK35_07840 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.34RR3 pKa = 11.84LNLAKK8 pKa = 9.77TLALTLIFSIIFMSCEE24 pKa = 3.53NDD26 pKa = 3.83DD27 pKa = 4.32NNTSNEE33 pKa = 4.21TMTSTYY39 pKa = 10.66LYY41 pKa = 10.05TSNNANGNITYY52 pKa = 10.52YY53 pKa = 11.03NVGDD57 pKa = 3.96MSNVMMTTLVTSSTAADD74 pKa = 3.83GVFYY78 pKa = 10.99DD79 pKa = 5.0ADD81 pKa = 4.27DD82 pKa = 4.58DD83 pKa = 4.3MLVQASRR90 pKa = 11.84SNMMLEE96 pKa = 4.19GFMDD100 pKa = 4.0TSMSSTGASISIGISGSSDD119 pKa = 2.73MMSPRR124 pKa = 11.84EE125 pKa = 3.71LAVNGDD131 pKa = 4.23YY132 pKa = 11.25YY133 pKa = 11.62VVADD137 pKa = 4.07NADD140 pKa = 3.22VDD142 pKa = 4.34GDD144 pKa = 4.31PDD146 pKa = 3.93TPDD149 pKa = 2.95GKK151 pKa = 10.94LYY153 pKa = 11.18VYY155 pKa = 10.01MKK157 pKa = 10.36DD158 pKa = 3.72GNDD161 pKa = 3.05FTLRR165 pKa = 11.84NVITTDD171 pKa = 2.67IKK173 pKa = 10.86LWGITFDD180 pKa = 4.37GEE182 pKa = 4.07SLYY185 pKa = 11.05AIVDD189 pKa = 3.84TTNEE193 pKa = 3.76LAVYY197 pKa = 10.87SNFLANTGDD206 pKa = 3.58MMLSATKK213 pKa = 9.71TIAVEE218 pKa = 4.8GIVRR222 pKa = 11.84THH224 pKa = 6.4GLTYY228 pKa = 10.88DD229 pKa = 3.48AMSDD233 pKa = 3.65TMVLTDD239 pKa = 4.14IAAAANGQDD248 pKa = 3.71DD249 pKa = 4.51GGFHH253 pKa = 7.69IITDD257 pKa = 4.45FMSKK261 pKa = 10.26FNNTADD267 pKa = 3.57GGTLAMSDD275 pKa = 3.91QIRR278 pKa = 11.84VAGSSTMLGNPVDD291 pKa = 3.67VAFDD295 pKa = 3.94SEE297 pKa = 4.83TEE299 pKa = 4.04TVYY302 pKa = 10.57IAEE305 pKa = 4.59AGNNGGRR312 pKa = 11.84ILAFNNIGSGGNIAPVLNYY331 pKa = 10.49DD332 pKa = 3.99LASASSVYY340 pKa = 9.98LVKK343 pKa = 10.92YY344 pKa = 10.24

Molecular weight: 36.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D7W3V5|A0A0D7W3V5_9FLAO Uncharacterized protein OS=Tamlana nanhaiensis OX=1382798 GN=PK35_03315 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.62LSVSSEE48 pKa = 4.03TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3029

0

3029

1091831

46

4626

360.5

40.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.604 ± 0.043

0.754 ± 0.013

5.589 ± 0.036

6.343 ± 0.035

5.275 ± 0.032

6.264 ± 0.038

1.767 ± 0.022

7.721 ± 0.038

7.758 ± 0.055

9.161 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.025 ± 0.017

6.828 ± 0.047

3.334 ± 0.02

3.331 ± 0.026

3.135 ± 0.026

6.389 ± 0.036

6.107 ± 0.048

6.356 ± 0.032

1.099 ± 0.018

4.16 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski