Thermoplasmatales archaeon SCGC AB-539-C06
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 780 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|N6WMI6|N6WMI6_9ARCH Uncharacterized protein (Fragment) OS=Thermoplasmatales archaeon SCGC AB-539-C06 OX=1242690 GN=MBGDC06_00790 PE=4 SV=1
MM1 pKa = 7.34 GKK3 pKa = 10.1 KK4 pKa = 10.02 SVILLLIGLLIFSNSGFNQIEE25 pKa = 3.98 NTKK28 pKa = 10.44 AVEE31 pKa = 4.05 TTFYY35 pKa = 11.31 VDD37 pKa = 5.02 DD38 pKa = 6.15 DD39 pKa = 4.13 GLEE42 pKa = 4.37 DD43 pKa = 3.8 YY44 pKa = 11.85 VNIQYY49 pKa = 10.85 AIDD52 pKa = 3.66 NASIGDD58 pKa = 3.82 TVYY61 pKa = 10.86 VYY63 pKa = 10.39 HH64 pKa = 7.4 GYY66 pKa = 10.4 YY67 pKa = 9.92 KK68 pKa = 10.84 EE69 pKa = 3.8 NLVINKK75 pKa = 9.52 SISLIGEE82 pKa = 4.17 DD83 pKa = 4.84 KK84 pKa = 11.46 GNTTIDD90 pKa = 3.21 GSNDD94 pKa = 2.92 VTVGIFSDD102 pKa = 3.94 NVEE105 pKa = 4.18 LTGFTIKK112 pKa = 10.79 NGTNGIMINGSSEE125 pKa = 4.21 TIVAEE130 pKa = 3.73 NTIIDD135 pKa = 4.16 TEE137 pKa = 4.17 YY138 pKa = 11.2 GIYY141 pKa = 10.29 VDD143 pKa = 5.25 NKK145 pKa = 9.43 SANNTIYY152 pKa = 11.22 NNNFLSNIQNAYY164 pKa = 10.38 DD165 pKa = 3.57 LSTNIWHH172 pKa = 6.27 YY173 pKa = 11.43 ASNGNYY179 pKa = 8.2 WDD181 pKa = 4.85 DD182 pKa = 3.68 YY183 pKa = 11.01 MGIDD187 pKa = 4.92 SDD189 pKa = 5.11 DD190 pKa = 3.85 NGIGDD195 pKa = 3.88 TPYY198 pKa = 10.96 NISGGNNQDD207 pKa = 3.23 IYY209 pKa = 11.17 PLMEE213 pKa = 5.96 PITEE217 pKa = 4.33 KK218 pKa = 10.68 PDD220 pKa = 3.37 ADD222 pKa = 3.82 FTYY225 pKa = 10.62 LPSNPTTQDD234 pKa = 3.14 VIQFNDD240 pKa = 3.05 TSTDD244 pKa = 3.23 LDD246 pKa = 4.23 GYY248 pKa = 9.41 IVSWSWDD255 pKa = 3.48 FGDD258 pKa = 5.06 GNISMEE264 pKa = 3.98 QNATHH269 pKa = 7.41 RR270 pKa = 11.84 YY271 pKa = 9.32 DD272 pKa = 4.3 DD273 pKa = 3.71 NGVYY277 pKa = 10.57 NVTLKK282 pKa = 9.34 ITDD285 pKa = 3.42 NYY287 pKa = 10.23 GATDD291 pKa = 4.42 EE292 pKa = 4.63 ISRR295 pKa = 11.84 QLGVLNVGPTADD307 pKa = 3.73 FVYY310 pKa = 10.82 SPLNPTDD317 pKa = 3.69 LQNVIFTDD325 pKa = 3.59 KK326 pKa = 11.27 SSDD329 pKa = 3.53 LDD331 pKa = 3.65 GDD333 pKa = 3.75 IVDD336 pKa = 4.68 WYY338 pKa = 10.1 WDD340 pKa = 3.72 LGDD343 pKa = 4.63 GNTSKK348 pKa = 10.72 LQSPSYY354 pKa = 10.57 QYY356 pKa = 11.51 GDD358 pKa = 3.15 NGTYY362 pKa = 10.06 IVTLTITDD370 pKa = 3.85 DD371 pKa = 4.35 DD372 pKa = 4.53 GVSEE376 pKa = 4.41 VTSQQISVSNVGPIIDD392 pKa = 3.77 FAFFSANVTIIVNDD406 pKa = 3.68 PVLFSDD412 pKa = 4.98 KK413 pKa = 11.4 SEE415 pKa = 4.77 DD416 pKa = 3.66 LDD418 pKa = 4.28 GDD420 pKa = 3.86 IVIWSWDD427 pKa = 3.61 FGDD430 pKa = 4.97 GATSTKK436 pKa = 10.2 KK437 pKa = 10.13 HH438 pKa = 5.51 PTHH441 pKa = 6.65 NYY443 pKa = 7.11 EE444 pKa = 4.11 KK445 pKa = 10.33 KK446 pKa = 9.11 GTYY449 pKa = 8.8 TVTLTVTDD457 pKa = 3.73 NDD459 pKa = 4.26 GVSSTKK465 pKa = 9.62 TKK467 pKa = 10.48 YY468 pKa = 8.77 ITVSVYY474 pKa = 10.27 VEE476 pKa = 3.9 PNEE479 pKa = 3.93 LVKK482 pKa = 10.95 GFSTFDD488 pKa = 2.82 IFFVVFLIIMVGLVIFLSRR507 pKa = 11.84 KK508 pKa = 9.02 YY509 pKa = 10.82 GG510 pKa = 3.35
Molecular weight: 56.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.554
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.452
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.215
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.923
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|N6VWM2|N6VWM2_9ARCH Acetyltransferase OS=Thermoplasmatales archaeon SCGC AB-539-C06 OX=1242690 GN=MBGDC06_00010 PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.23 IKK4 pKa = 9.33 TRR6 pKa = 11.84 KK7 pKa = 8.94 KK8 pKa = 8.48 VYY10 pKa = 10.4 GRR12 pKa = 11.84 IKK14 pKa = 9.73 GCEE17 pKa = 3.3 RR18 pKa = 11.84 CGRR21 pKa = 11.84 KK22 pKa = 9.31 RR23 pKa = 11.84 GLIRR27 pKa = 11.84 RR28 pKa = 11.84 YY29 pKa = 9.8 GLHH32 pKa = 7.31 LCRR35 pKa = 11.84 QCFRR39 pKa = 11.84 EE40 pKa = 3.94 KK41 pKa = 10.93 AEE43 pKa = 4.21 EE44 pKa = 3.99 IGFKK48 pKa = 10.37 KK49 pKa = 10.85 YY50 pKa = 10.21 SS51 pKa = 3.24
Molecular weight: 6.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.736
IPC_protein 10.175
Toseland 10.701
ProMoST 10.292
Dawson 10.804
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 11.228
Grimsley 10.833
Solomon 10.862
Lehninger 10.847
Nozaki 10.701
DTASelect 10.438
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.73
Patrickios 10.965
IPC_peptide 10.877
IPC2_peptide 9.531
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
780
0
780
164750
22
1147
211.2
23.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.658 ± 0.125
1.339 ± 0.05
5.845 ± 0.1
6.778 ± 0.116
4.522 ± 0.081
6.987 ± 0.11
1.818 ± 0.038
9.249 ± 0.12
8.407 ± 0.147
8.456 ± 0.117
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.562 ± 0.049
5.17 ± 0.106
3.635 ± 0.058
2.452 ± 0.05
3.775 ± 0.074
6.281 ± 0.078
5.423 ± 0.095
6.608 ± 0.089
1.212 ± 0.042
3.824 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here