fungal sp. No.11243
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9692 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S6XIG4|A0A0S6XIG4_9FUNG TPT domain-containing protein OS=fungal sp. No.11243 OX=1603295 GN=ANO11243_036810 PE=4 SV=1
MM1 pKa = 6.7 MFKK4 pKa = 10.43 RR5 pKa = 11.84 IYY7 pKa = 10.41 ALNAIAASLLTLTKK21 pKa = 9.92 PGDD24 pKa = 3.93 GASVGQPSASTQVCTNTQPVGGPTVTVTQSPVTVTPPTVTITPSMVVPTVTVIRR78 pKa = 11.84 QVNEE82 pKa = 3.92 TEE84 pKa = 4.14 NKK86 pKa = 9.97 IGTLYY91 pKa = 9.06 KK92 pKa = 10.04 TLSDD96 pKa = 4.38 VFTQTNSLVVVTTVISDD113 pKa = 3.74 AFMTATAVNTITATQTNVVSVFTTVISDD141 pKa = 3.64 STVVSTVTNDD151 pKa = 3.05 ATVTQTNSVTLVSTVTSDD169 pKa = 3.4 VVSISTVTSDD179 pKa = 3.51 VVSVSTVVSVVTSTVSSGNASNTGIAWAWYY209 pKa = 8.44 NAASLGSEE217 pKa = 4.09 WNDD220 pKa = 2.68 GSTWVPNAISDD231 pKa = 3.89 LTANGTGVLTTQMGFSIINAVGSTMQMYY259 pKa = 10.76 DD260 pKa = 3.32 DD261 pKa = 5.62 TITSVQNMGIMYY273 pKa = 8.43 YY274 pKa = 11.23 ANISCTQNGTYY285 pKa = 10.57 DD286 pKa = 3.56 FQFNNVDD293 pKa = 3.49 DD294 pKa = 4.49 ATAIWVGDD302 pKa = 3.7 IANAPTWEE310 pKa = 4.46 TTSPTTTFTDD320 pKa = 2.98 NRR322 pKa = 11.84 RR323 pKa = 11.84 QDD325 pKa = 4.23 DD326 pKa = 4.4 YY327 pKa = 10.1 MTLASAEE334 pKa = 4.27 CTAGDD339 pKa = 3.64 SLAFRR344 pKa = 11.84 WVFANFCCGLIFGPRR359 pKa = 11.84 IVDD362 pKa = 3.36 PSGSILVDD370 pKa = 3.36 TQNMYY375 pKa = 11.25 SSLFSFTRR383 pKa = 3.38
Molecular weight: 40.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.592
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.668
EMBOSS 3.834
Sillero 3.961
Patrickios 0.769
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A0S6XGP3|A0A0S6XGP3_9FUNG Endonuclease OS=fungal sp. No.11243 OX=1603295 GN=ANO11243_028990 PE=3 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.15 WRR45 pKa = 11.84 KK46 pKa = 7.38 TRR48 pKa = 11.84 IGII51 pKa = 4.0
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9692
0
9692
4222938
37
15639
435.7
48.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.31 ± 0.024
1.262 ± 0.01
5.811 ± 0.018
5.784 ± 0.025
3.559 ± 0.015
6.916 ± 0.025
2.474 ± 0.01
4.619 ± 0.016
4.618 ± 0.023
8.824 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.01
3.383 ± 0.013
5.859 ± 0.028
4.049 ± 0.019
6.462 ± 0.022
8.5 ± 0.032
6.157 ± 0.025
6.191 ± 0.019
1.41 ± 0.009
2.594 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here