Nonomuraea wenchangensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9568 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1I0LPK3|A0A1I0LPK3_9ACTN Fatty acid CoA ligase FadD32 OS=Nonomuraea wenchangensis OX=568860 GN=SAMN05421811_121128 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84VRR4 pKa = 11.84VDD6 pKa = 3.31LTLCQTHH13 pKa = 6.04AQCVFAAPEE22 pKa = 4.1VFALDD27 pKa = 4.56DD28 pKa = 4.75DD29 pKa = 5.23DD30 pKa = 5.0EE31 pKa = 4.63LVYY34 pKa = 10.95DD35 pKa = 4.7ATPGDD40 pKa = 4.07ASWPAVEE47 pKa = 4.36QAARR51 pKa = 11.84ACPVQAIFLDD61 pKa = 4.36GDD63 pKa = 3.63

Molecular weight:
6.84 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1I0LMZ2|A0A1I0LMZ2_9ACTN RNA polymerase sigma-70 factor ECF subfamily OS=Nonomuraea wenchangensis OX=568860 GN=SAMN05421811_12096 PE=3 SV=1
MM1 pKa = 6.33QWRR4 pKa = 11.84RR5 pKa = 11.84SGYY8 pKa = 8.05TSSLRR13 pKa = 11.84SSASRR18 pKa = 11.84ARR20 pKa = 11.84SSWPSSKK27 pKa = 9.76RR28 pKa = 11.84WGSSSVRR35 pKa = 11.84RR36 pKa = 11.84PPPSRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84WSAGSPKK50 pKa = 9.92LWAHH54 pKa = 6.11RR55 pKa = 11.84RR56 pKa = 11.84VGPPGAADD64 pKa = 3.37SRR66 pKa = 11.84PTGRR70 pKa = 11.84SRR72 pKa = 11.84QGLRR76 pKa = 11.84TGRR79 pKa = 11.84RR80 pKa = 11.84KK81 pKa = 9.78ARR83 pKa = 11.84PATVPGRR90 pKa = 11.84PRR92 pKa = 11.84CLLLRR97 pKa = 11.84VRR99 pKa = 11.84APGPVRR105 pKa = 11.84VRR107 pKa = 11.84PRR109 pKa = 11.84RR110 pKa = 11.84AALRR114 pKa = 11.84VRR116 pKa = 11.84RR117 pKa = 11.84GPRR120 pKa = 11.84ARR122 pKa = 11.84RR123 pKa = 11.84VRR125 pKa = 11.84KK126 pKa = 9.22VLRR129 pKa = 11.84AAASPARR136 pKa = 11.84RR137 pKa = 11.84FPCLTSSSRR146 pKa = 11.84RR147 pKa = 11.84PRR149 pKa = 11.84AVRR152 pKa = 11.84RR153 pKa = 11.84PRR155 pKa = 11.84LRR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84ASRR162 pKa = 11.84AARR165 pKa = 11.84RR166 pKa = 11.84PGRR169 pKa = 11.84PRR171 pKa = 11.84VRR173 pKa = 11.84VPARR177 pKa = 11.84LRR179 pKa = 11.84VRR181 pKa = 11.84VPAPSRR187 pKa = 11.84ARR189 pKa = 11.84VPPSRR194 pKa = 11.84AAAARR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84RR202 pKa = 11.84RR203 pKa = 11.84VLAARR208 pKa = 11.84PPAAARR214 pKa = 11.84VPAPSRR220 pKa = 11.84ARR222 pKa = 11.84VARR225 pKa = 11.84VRR227 pKa = 11.84ATTRR231 pKa = 11.84SRR233 pKa = 11.84PTPAAWARR241 pKa = 11.84RR242 pKa = 11.84PGRR245 pKa = 11.84SVRR248 pKa = 11.84AAASVTVAPARR259 pKa = 11.84VASAVTARR267 pKa = 11.84RR268 pKa = 11.84ATARR272 pKa = 11.84CRR274 pKa = 11.84VRR276 pKa = 11.84RR277 pKa = 11.84RR278 pKa = 11.84VAAATGPLPARR289 pKa = 11.84VPVRR293 pKa = 11.84PPAARR298 pKa = 11.84VRR300 pKa = 11.84VPAPVAPVVRR310 pKa = 11.84VPVVRR315 pKa = 11.84VPTRR319 pKa = 3.06

Molecular weight:
35.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9568

0

9568

3041378

27

5134

317.9

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.689 ± 0.041

0.783 ± 0.007

5.71 ± 0.019

5.737 ± 0.025

2.771 ± 0.014

9.454 ± 0.027

2.205 ± 0.012

3.317 ± 0.018

1.958 ± 0.019

10.778 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.853 ± 0.01

1.725 ± 0.014

6.13 ± 0.021

2.618 ± 0.017

8.337 ± 0.028

4.782 ± 0.017

5.716 ± 0.02

8.717 ± 0.026

1.595 ± 0.012

2.126 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski