Nonomuraea wenchangensis
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9568 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I0LPK3|A0A1I0LPK3_9ACTN Fatty acid CoA ligase FadD32 OS=Nonomuraea wenchangensis OX=568860 GN=SAMN05421811_121128 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 VRR4 pKa = 11.84 VDD6 pKa = 3.31 LTLCQTHH13 pKa = 6.04 AQCVFAAPEE22 pKa = 4.1 VFALDD27 pKa = 4.56 DD28 pKa = 4.75 DD29 pKa = 5.23 DD30 pKa = 5.0 EE31 pKa = 4.63 LVYY34 pKa = 10.95 DD35 pKa = 4.7 ATPGDD40 pKa = 4.07 ASWPAVEE47 pKa = 4.36 QAARR51 pKa = 11.84 ACPVQAIFLDD61 pKa = 4.36 GDD63 pKa = 3.63
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.77
IPC_protein 3.706
Toseland 3.49
ProMoST 3.821
Dawson 3.719
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.834
Patrickios 1.888
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A1I0LMZ2|A0A1I0LMZ2_9ACTN RNA polymerase sigma-70 factor ECF subfamily OS=Nonomuraea wenchangensis OX=568860 GN=SAMN05421811_12096 PE=3 SV=1
MM1 pKa = 6.33 QWRR4 pKa = 11.84 RR5 pKa = 11.84 SGYY8 pKa = 8.05 TSSLRR13 pKa = 11.84 SSASRR18 pKa = 11.84 ARR20 pKa = 11.84 SSWPSSKK27 pKa = 9.76 RR28 pKa = 11.84 WGSSSVRR35 pKa = 11.84 RR36 pKa = 11.84 PPPSRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 WSAGSPKK50 pKa = 9.92 LWAHH54 pKa = 6.11 RR55 pKa = 11.84 RR56 pKa = 11.84 VGPPGAADD64 pKa = 3.37 SRR66 pKa = 11.84 PTGRR70 pKa = 11.84 SRR72 pKa = 11.84 QGLRR76 pKa = 11.84 TGRR79 pKa = 11.84 RR80 pKa = 11.84 KK81 pKa = 9.78 ARR83 pKa = 11.84 PATVPGRR90 pKa = 11.84 PRR92 pKa = 11.84 CLLLRR97 pKa = 11.84 VRR99 pKa = 11.84 APGPVRR105 pKa = 11.84 VRR107 pKa = 11.84 PRR109 pKa = 11.84 RR110 pKa = 11.84 AALRR114 pKa = 11.84 VRR116 pKa = 11.84 RR117 pKa = 11.84 GPRR120 pKa = 11.84 ARR122 pKa = 11.84 RR123 pKa = 11.84 VRR125 pKa = 11.84 KK126 pKa = 9.22 VLRR129 pKa = 11.84 AAASPARR136 pKa = 11.84 RR137 pKa = 11.84 FPCLTSSSRR146 pKa = 11.84 RR147 pKa = 11.84 PRR149 pKa = 11.84 AVRR152 pKa = 11.84 RR153 pKa = 11.84 PRR155 pKa = 11.84 LRR157 pKa = 11.84 RR158 pKa = 11.84 RR159 pKa = 11.84 ASRR162 pKa = 11.84 AARR165 pKa = 11.84 RR166 pKa = 11.84 PGRR169 pKa = 11.84 PRR171 pKa = 11.84 VRR173 pKa = 11.84 VPARR177 pKa = 11.84 LRR179 pKa = 11.84 VRR181 pKa = 11.84 VPAPSRR187 pKa = 11.84 ARR189 pKa = 11.84 VPPSRR194 pKa = 11.84 AAAARR199 pKa = 11.84 RR200 pKa = 11.84 RR201 pKa = 11.84 RR202 pKa = 11.84 RR203 pKa = 11.84 VLAARR208 pKa = 11.84 PPAAARR214 pKa = 11.84 VPAPSRR220 pKa = 11.84 ARR222 pKa = 11.84 VARR225 pKa = 11.84 VRR227 pKa = 11.84 ATTRR231 pKa = 11.84 SRR233 pKa = 11.84 PTPAAWARR241 pKa = 11.84 RR242 pKa = 11.84 PGRR245 pKa = 11.84 SVRR248 pKa = 11.84 AAASVTVAPARR259 pKa = 11.84 VASAVTARR267 pKa = 11.84 RR268 pKa = 11.84 ATARR272 pKa = 11.84 CRR274 pKa = 11.84 VRR276 pKa = 11.84 RR277 pKa = 11.84 RR278 pKa = 11.84 VAAATGPLPARR289 pKa = 11.84 VPVRR293 pKa = 11.84 PPAARR298 pKa = 11.84 VRR300 pKa = 11.84 VPAPVAPVVRR310 pKa = 11.84 VPVVRR315 pKa = 11.84 VPTRR319 pKa = 3.06
Molecular weight: 35.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.418
IPC_protein 12.998
Toseland 13.159
ProMoST 13.656
Dawson 13.159
Bjellqvist 13.159
Wikipedia 13.627
Rodwell 12.676
Grimsley 13.188
Solomon 13.656
Lehninger 13.554
Nozaki 13.159
DTASelect 13.159
Thurlkill 13.159
EMBOSS 13.656
Sillero 13.159
Patrickios 12.369
IPC_peptide 13.656
IPC2_peptide 12.647
IPC2.peptide.svr19 9.308
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9568
0
9568
3041378
27
5134
317.9
34.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.689 ± 0.041
0.783 ± 0.007
5.71 ± 0.019
5.737 ± 0.025
2.771 ± 0.014
9.454 ± 0.027
2.205 ± 0.012
3.317 ± 0.018
1.958 ± 0.019
10.778 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.853 ± 0.01
1.725 ± 0.014
6.13 ± 0.021
2.618 ± 0.017
8.337 ± 0.028
4.782 ± 0.017
5.716 ± 0.02
8.717 ± 0.026
1.595 ± 0.012
2.126 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here