Elstera cyanobacteriorum
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3712 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A255XI83|A0A255XI83_9PROT Uncharacterized protein OS=Elstera cyanobacteriorum OX=2022747 GN=CHR90_16890 PE=4 SV=1
MM1 pKa = 7.58 AVQGGQVSLTDD12 pKa = 4.67 LDD14 pKa = 4.06 PGVYY18 pKa = 10.21 LVTPSGAFDD27 pKa = 4.1 LVFPYY32 pKa = 10.83 DD33 pKa = 3.41 EE34 pKa = 5.3 GDD36 pKa = 3.35 FHH38 pKa = 9.1 LSDD41 pKa = 3.04 IFDD44 pKa = 3.71 YY45 pKa = 11.4 FEE47 pKa = 5.33 LGEE50 pKa = 4.05 VLEE53 pKa = 4.58 EE54 pKa = 4.79 DD55 pKa = 3.82 GAPGIEE61 pKa = 3.89 LDD63 pKa = 3.6 ARR65 pKa = 11.84 EE66 pKa = 4.16 VKK68 pKa = 9.76 QLKK71 pKa = 10.22 RR72 pKa = 11.84 MARR75 pKa = 11.84 EE76 pKa = 3.96 YY77 pKa = 11.3 ADD79 pKa = 3.57 DD80 pKa = 4.53 HH81 pKa = 6.45 PPEE84 pKa = 5.08 FIAMCRR90 pKa = 11.84 AMVQAVADD98 pKa = 4.01 TAPDD102 pKa = 3.46 EE103 pKa = 5.05 DD104 pKa = 4.31 VVCFYY109 pKa = 11.51 EE110 pKa = 4.53 NFGG113 pKa = 3.73
Molecular weight: 12.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 1.863
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A255XIW3|A0A255XIW3_9PROT Uncharacterized protein OS=Elstera cyanobacteriorum OX=2022747 GN=CHR90_18185 PE=4 SV=1
MM1 pKa = 7.48 ASVRR5 pKa = 11.84 AAQAAVSARR14 pKa = 11.84 RR15 pKa = 11.84 GTGPSARR22 pKa = 11.84 VRR24 pKa = 11.84 RR25 pKa = 11.84 ATARR29 pKa = 11.84 KK30 pKa = 9.59 GIAPRR35 pKa = 11.84 GNGVSARR42 pKa = 11.84 APRR45 pKa = 11.84 EE46 pKa = 3.43 TARR49 pKa = 11.84 AVRR52 pKa = 11.84 VHH54 pKa = 6.5 RR55 pKa = 11.84 AMVRR59 pKa = 11.84 AVTARR64 pKa = 11.84 AASVRR69 pKa = 11.84 STTARR74 pKa = 11.84 KK75 pKa = 7.09 PTASRR80 pKa = 11.84 ASVASARR87 pKa = 11.84 APRR90 pKa = 11.84 GTVHH94 pKa = 7.49 AAQGQKK100 pKa = 10.69 VIVHH104 pKa = 6.89 AAQGQKK110 pKa = 10.69 VIVHH114 pKa = 6.89 AAQGQKK120 pKa = 10.69 VIVHH124 pKa = 6.77 AAQGRR129 pKa = 11.84 RR130 pKa = 11.84 ATAA133 pKa = 3.34
Molecular weight: 13.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.603
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.31
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.199
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3712
0
3712
1198734
41
3429
322.9
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.453 ± 0.066
0.78 ± 0.011
5.55 ± 0.032
5.021 ± 0.029
3.576 ± 0.024
8.555 ± 0.041
1.871 ± 0.017
4.987 ± 0.032
3.102 ± 0.033
11.359 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.019
2.294 ± 0.024
5.724 ± 0.04
3.422 ± 0.025
6.939 ± 0.044
4.867 ± 0.027
5.731 ± 0.034
7.163 ± 0.03
1.317 ± 0.016
2.138 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here