Amino acid dipepetide frequency for Lactococcus phage AM4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.18AlaAla: 0.18 ± 0.078
0.206AlaCys: 0.206 ± 0.079
2.676AlaAsp: 2.676 ± 0.259
3.473AlaGlu: 3.473 ± 0.313
1.775AlaPhe: 1.775 ± 0.22
3.602AlaGly: 3.602 ± 0.596
0.849AlaHis: 0.849 ± 0.154
3.525AlaIle: 3.525 ± 0.347
5.069AlaLys: 5.069 ± 0.485
4.503AlaLeu: 4.503 ± 0.492
1.132AlaMet: 1.132 ± 0.164
3.37AlaAsn: 3.37 ± 0.313
0.926AlaPro: 0.926 ± 0.205
2.393AlaGln: 2.393 ± 0.35
1.801AlaArg: 1.801 ± 0.211
3.705AlaSer: 3.705 ± 0.477
3.216AlaThr: 3.216 ± 0.405
3.653AlaVal: 3.653 ± 0.391
0.566AlaTrp: 0.566 ± 0.13
2.959AlaTyr: 2.959 ± 0.279
0.0AlaXaa: 0.0 ± 0.0
Cys
0.18CysAla: 0.18 ± 0.07
0.077CysCys: 0.077 ± 0.043
0.463CysAsp: 0.463 ± 0.144
0.489CysGlu: 0.489 ± 0.147
0.334CysPhe: 0.334 ± 0.091
0.489CysGly: 0.489 ± 0.147
0.026CysHis: 0.026 ± 0.029
0.463CysIle: 0.463 ± 0.113
0.695CysLys: 0.695 ± 0.181
0.437CysLeu: 0.437 ± 0.117
0.103CysMet: 0.103 ± 0.049
0.489CysAsn: 0.489 ± 0.124
0.412CysPro: 0.412 ± 0.174
0.206CysGln: 0.206 ± 0.087
0.437CysArg: 0.437 ± 0.119
0.566CysSer: 0.566 ± 0.147
0.412CysThr: 0.412 ± 0.1
0.489CysVal: 0.489 ± 0.13
0.103CysTrp: 0.103 ± 0.053
0.283CysTyr: 0.283 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
2.804AspAla: 2.804 ± 0.441
0.257AspCys: 0.257 ± 0.08
4.554AspAsp: 4.554 ± 0.342
6.201AspGlu: 6.201 ± 0.429
4.348AspPhe: 4.348 ± 0.369
4.786AspGly: 4.786 ± 0.339
0.54AspHis: 0.54 ± 0.141
5.943AspIle: 5.943 ± 0.416
7.178AspLys: 7.178 ± 0.413
5.66AspLeu: 5.66 ± 0.488
1.672AspMet: 1.672 ± 0.225
4.786AspAsn: 4.786 ± 0.315
0.798AspPro: 0.798 ± 0.138
0.926AspGln: 0.926 ± 0.177
1.981AspArg: 1.981 ± 0.263
4.297AspSer: 4.297 ± 0.378
4.039AspThr: 4.039 ± 0.322
3.859AspVal: 3.859 ± 0.34
0.772AspTrp: 0.772 ± 0.137
2.959AspTyr: 2.959 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
3.988GluAla: 3.988 ± 0.322
0.463GluCys: 0.463 ± 0.121
5.712GluAsp: 5.712 ± 0.519
6.741GluGlu: 6.741 ± 0.555
3.448GluPhe: 3.448 ± 0.359
3.602GluGly: 3.602 ± 0.296
1.364GluHis: 1.364 ± 0.245
7.178GluIle: 7.178 ± 0.515
7.281GluLys: 7.281 ± 0.46
7.796GluLeu: 7.796 ± 0.499
2.213GluMet: 2.213 ± 0.232
4.708GluAsn: 4.708 ± 0.356
1.106GluPro: 1.106 ± 0.171
2.47GluGln: 2.47 ± 0.274
2.521GluArg: 2.521 ± 0.254
4.966GluSer: 4.966 ± 0.371
3.859GluThr: 3.859 ± 0.31
4.811GluVal: 4.811 ± 0.394
0.746GluTrp: 0.746 ± 0.127
4.322GluTyr: 4.322 ± 0.501
0.0GluXaa: 0.0 ± 0.0
Phe
2.135PheAla: 2.135 ± 0.207
0.386PheCys: 0.386 ± 0.112
3.808PheAsp: 3.808 ± 0.339
3.885PheGlu: 3.885 ± 0.427
1.492PhePhe: 1.492 ± 0.248
2.779PheGly: 2.779 ± 0.312
0.669PheHis: 0.669 ± 0.177
3.087PheIle: 3.087 ± 0.317
4.374PheLys: 4.374 ± 0.368
3.37PheLeu: 3.37 ± 0.294
1.286PheMet: 1.286 ± 0.182
3.01PheAsn: 3.01 ± 0.317
0.669PhePro: 0.669 ± 0.124
1.312PheGln: 1.312 ± 0.18
1.544PheArg: 1.544 ± 0.237
2.907PheSer: 2.907 ± 0.313
2.907PheThr: 2.907 ± 0.265
2.521PheVal: 2.521 ± 0.257
0.489PheTrp: 0.489 ± 0.125
2.058PheTyr: 2.058 ± 0.252
0.0PheXaa: 0.0 ± 0.0
Gly
2.65GlyAla: 2.65 ± 0.373
0.386GlyCys: 0.386 ± 0.099
3.448GlyAsp: 3.448 ± 0.257
3.859GlyGlu: 3.859 ± 0.338
3.113GlyPhe: 3.113 ± 0.268
3.448GlyGly: 3.448 ± 0.46
0.901GlyHis: 0.901 ± 0.17
4.966GlyIle: 4.966 ± 0.387
4.811GlyLys: 4.811 ± 0.41
4.683GlyLeu: 4.683 ± 0.332
1.518GlyMet: 1.518 ± 0.155
3.937GlyAsn: 3.937 ± 0.403
0.129GlyPro: 0.129 ± 0.057
1.518GlyGln: 1.518 ± 0.184
2.161GlyArg: 2.161 ± 0.209
3.756GlySer: 3.756 ± 0.319
3.911GlyThr: 3.911 ± 0.381
3.859GlyVal: 3.859 ± 0.284
0.823GlyTrp: 0.823 ± 0.171
3.293GlyTyr: 3.293 ± 0.293
0.0GlyXaa: 0.0 ± 0.0
His
0.669HisAla: 0.669 ± 0.118
0.077HisCys: 0.077 ± 0.044
1.055HisAsp: 1.055 ± 0.17
1.055HisGlu: 1.055 ± 0.183
0.669HisPhe: 0.669 ± 0.159
1.106HisGly: 1.106 ± 0.219
0.232HisHis: 0.232 ± 0.076
1.209HisIle: 1.209 ± 0.203
1.184HisLys: 1.184 ± 0.175
1.132HisLeu: 1.132 ± 0.186
0.386HisMet: 0.386 ± 0.095
1.081HisAsn: 1.081 ± 0.241
0.36HisPro: 0.36 ± 0.09
0.566HisGln: 0.566 ± 0.125
0.515HisArg: 0.515 ± 0.1
1.003HisSer: 1.003 ± 0.165
0.72HisThr: 0.72 ± 0.12
0.617HisVal: 0.617 ± 0.114
0.18HisTrp: 0.18 ± 0.065
0.823HisTyr: 0.823 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
3.962IleAla: 3.962 ± 0.311
0.566IleCys: 0.566 ± 0.121
5.738IleAsp: 5.738 ± 0.316
6.175IleGlu: 6.175 ± 0.454
3.139IlePhe: 3.139 ± 0.323
3.576IleGly: 3.576 ± 0.306
1.055IleHis: 1.055 ± 0.197
5.712IleIle: 5.712 ± 0.523
7.487IleLys: 7.487 ± 0.419
5.686IleLeu: 5.686 ± 0.425
1.467IleMet: 1.467 ± 0.165
5.609IleAsn: 5.609 ± 0.345
2.496IlePro: 2.496 ± 0.277
2.496IleGln: 2.496 ± 0.324
2.316IleArg: 2.316 ± 0.224
5.583IleSer: 5.583 ± 0.389
4.58IleThr: 4.58 ± 0.405
5.274IleVal: 5.274 ± 0.48
0.695IleTrp: 0.695 ± 0.132
3.087IleTyr: 3.087 ± 0.366
0.0IleXaa: 0.0 ± 0.0
Lys
5.171LysAla: 5.171 ± 0.503
0.798LysCys: 0.798 ± 0.216
6.689LysAsp: 6.689 ± 0.423
9.52LysGlu: 9.52 ± 0.519
3.834LysPhe: 3.834 ± 0.394
4.168LysGly: 4.168 ± 0.273
1.569LysHis: 1.569 ± 0.25
6.998LysIle: 6.998 ± 0.411
7.976LysLys: 7.976 ± 0.578
8.259LysLeu: 8.259 ± 0.473
3.036LysMet: 3.036 ± 0.318
5.815LysAsn: 5.815 ± 0.366
2.058LysPro: 2.058 ± 0.227
3.216LysGln: 3.216 ± 0.35
3.679LysArg: 3.679 ± 0.292
4.811LysSer: 4.811 ± 0.516
5.171LysThr: 5.171 ± 0.329
5.12LysVal: 5.12 ± 0.265
1.158LysTrp: 1.158 ± 0.169
3.885LysTyr: 3.885 ± 0.298
0.0LysXaa: 0.0 ± 0.0
Leu
4.991LeuAla: 4.991 ± 0.397
0.72LeuCys: 0.72 ± 0.179
5.892LeuAsp: 5.892 ± 0.368
6.535LeuGlu: 6.535 ± 0.52
3.679LeuPhe: 3.679 ± 0.403
5.069LeuGly: 5.069 ± 0.51
1.055LeuHis: 1.055 ± 0.167
5.918LeuIle: 5.918 ± 0.479
8.31LeuLys: 8.31 ± 0.442
6.355LeuLeu: 6.355 ± 0.467
1.981LeuMet: 1.981 ± 0.227
5.377LeuAsn: 5.377 ± 0.368
2.161LeuPro: 2.161 ± 0.213
2.624LeuGln: 2.624 ± 0.365
2.702LeuArg: 2.702 ± 0.331
6.072LeuSer: 6.072 ± 0.423
5.48LeuThr: 5.48 ± 0.395
4.477LeuVal: 4.477 ± 0.38
0.643LeuTrp: 0.643 ± 0.137
3.19LeuTyr: 3.19 ± 0.362
0.0LeuXaa: 0.0 ± 0.0
Met
1.621MetAla: 1.621 ± 0.183
0.129MetCys: 0.129 ± 0.055
1.647MetAsp: 1.647 ± 0.244
1.621MetGlu: 1.621 ± 0.214
0.926MetPhe: 0.926 ± 0.141
1.312MetGly: 1.312 ± 0.193
0.154MetHis: 0.154 ± 0.059
1.595MetIle: 1.595 ± 0.177
2.547MetLys: 2.547 ± 0.255
1.878MetLeu: 1.878 ± 0.251
0.695MetMet: 0.695 ± 0.116
2.084MetAsn: 2.084 ± 0.225
0.823MetPro: 0.823 ± 0.131
0.926MetGln: 0.926 ± 0.223
0.798MetArg: 0.798 ± 0.137
1.955MetSer: 1.955 ± 0.255
1.801MetThr: 1.801 ± 0.198
1.544MetVal: 1.544 ± 0.171
0.283MetTrp: 0.283 ± 0.085
1.106MetTyr: 1.106 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.19AsnAla: 3.19 ± 0.394
0.386AsnCys: 0.386 ± 0.105
3.525AsnAsp: 3.525 ± 0.311
5.274AsnGlu: 5.274 ± 0.431
2.419AsnPhe: 2.419 ± 0.282
4.297AsnGly: 4.297 ± 0.335
0.978AsnHis: 0.978 ± 0.184
4.991AsnIle: 4.991 ± 0.301
6.149AsnLys: 6.149 ± 0.45
5.635AsnLeu: 5.635 ± 0.343
1.364AsnMet: 1.364 ± 0.183
3.653AsnAsn: 3.653 ± 0.297
2.058AsnPro: 2.058 ± 0.195
2.907AsnGln: 2.907 ± 0.413
2.161AsnArg: 2.161 ± 0.283
4.451AsnSer: 4.451 ± 0.344
3.628AsnThr: 3.628 ± 0.27
3.988AsnVal: 3.988 ± 0.308
0.592AsnTrp: 0.592 ± 0.112
2.779AsnTyr: 2.779 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
1.081ProAla: 1.081 ± 0.159
0.103ProCys: 0.103 ± 0.052
1.672ProAsp: 1.672 ± 0.206
1.467ProGlu: 1.467 ± 0.147
1.081ProPhe: 1.081 ± 0.172
0.077ProGly: 0.077 ± 0.05
0.386ProHis: 0.386 ± 0.089
1.621ProIle: 1.621 ± 0.184
2.213ProLys: 2.213 ± 0.326
1.981ProLeu: 1.981 ± 0.213
0.643ProMet: 0.643 ± 0.129
1.595ProAsn: 1.595 ± 0.246
0.309ProPro: 0.309 ± 0.079
0.72ProGln: 0.72 ± 0.125
0.566ProArg: 0.566 ± 0.118
1.492ProSer: 1.492 ± 0.202
1.672ProThr: 1.672 ± 0.238
2.007ProVal: 2.007 ± 0.254
0.129ProTrp: 0.129 ± 0.068
1.338ProTyr: 1.338 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
1.93GlnAla: 1.93 ± 0.377
0.206GlnCys: 0.206 ± 0.078
1.724GlnAsp: 1.724 ± 0.194
2.213GlnGlu: 2.213 ± 0.225
1.338GlnPhe: 1.338 ± 0.172
1.878GlnGly: 1.878 ± 0.225
0.849GlnHis: 0.849 ± 0.181
2.47GlnIle: 2.47 ± 0.261
2.753GlnLys: 2.753 ± 0.35
3.216GlnLeu: 3.216 ± 0.466
1.235GlnMet: 1.235 ± 0.285
1.878GlnAsn: 1.878 ± 0.238
0.772GlnPro: 0.772 ± 0.144
1.441GlnGln: 1.441 ± 0.467
1.569GlnArg: 1.569 ± 0.189
1.827GlnSer: 1.827 ± 0.239
2.341GlnThr: 2.341 ± 0.344
1.338GlnVal: 1.338 ± 0.18
0.334GlnTrp: 0.334 ± 0.097
1.621GlnTyr: 1.621 ± 0.204
0.0GlnXaa: 0.0 ± 0.0
Arg
1.286ArgAla: 1.286 ± 0.187
0.386ArgCys: 0.386 ± 0.19
2.624ArgAsp: 2.624 ± 0.217
2.985ArgGlu: 2.985 ± 0.249
1.621ArgPhe: 1.621 ± 0.193
2.161ArgGly: 2.161 ± 0.252
0.515ArgHis: 0.515 ± 0.108
2.65ArgIle: 2.65 ± 0.284
3.01ArgLys: 3.01 ± 0.311
2.599ArgLeu: 2.599 ± 0.229
0.798ArgMet: 0.798 ± 0.126
2.547ArgAsn: 2.547 ± 0.228
0.617ArgPro: 0.617 ± 0.14
1.106ArgGln: 1.106 ± 0.191
1.003ArgArg: 1.003 ± 0.171
1.827ArgSer: 1.827 ± 0.242
1.878ArgThr: 1.878 ± 0.205
2.341ArgVal: 2.341 ± 0.284
0.592ArgTrp: 0.592 ± 0.117
2.058ArgTyr: 2.058 ± 0.217
0.0ArgXaa: 0.0 ± 0.0
Ser
3.628SerAla: 3.628 ± 0.447
0.463SerCys: 0.463 ± 0.129
4.58SerAsp: 4.58 ± 0.415
5.043SerGlu: 5.043 ± 0.353
3.319SerPhe: 3.319 ± 0.316
4.322SerGly: 4.322 ± 0.394
0.849SerHis: 0.849 ± 0.171
5.429SerIle: 5.429 ± 0.51
6.098SerLys: 6.098 ± 0.392
5.069SerLeu: 5.069 ± 0.457
1.415SerMet: 1.415 ± 0.147
3.396SerAsn: 3.396 ± 0.395
1.544SerPro: 1.544 ± 0.185
2.058SerGln: 2.058 ± 0.249
2.573SerArg: 2.573 ± 0.272
4.914SerSer: 4.914 ± 0.712
4.091SerThr: 4.091 ± 0.302
3.988SerVal: 3.988 ± 0.332
0.669SerTrp: 0.669 ± 0.134
2.573SerTyr: 2.573 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
3.602ThrAla: 3.602 ± 0.415
0.386ThrCys: 0.386 ± 0.118
4.014ThrAsp: 4.014 ± 0.331
3.628ThrGlu: 3.628 ± 0.372
2.804ThrPhe: 2.804 ± 0.294
3.602ThrGly: 3.602 ± 0.41
1.003ThrHis: 1.003 ± 0.165
4.58ThrIle: 4.58 ± 0.436
5.506ThrLys: 5.506 ± 0.321
5.583ThrLeu: 5.583 ± 0.364
1.492ThrMet: 1.492 ± 0.179
3.551ThrAsn: 3.551 ± 0.302
2.135ThrPro: 2.135 ± 0.24
1.878ThrGln: 1.878 ± 0.223
2.11ThrArg: 2.11 ± 0.248
4.091ThrSer: 4.091 ± 0.301
3.448ThrThr: 3.448 ± 0.336
4.76ThrVal: 4.76 ± 0.353
0.695ThrTrp: 0.695 ± 0.133
2.496ThrTyr: 2.496 ± 0.257
0.0ThrXaa: 0.0 ± 0.0
Val
3.602ValAla: 3.602 ± 0.4
0.617ValCys: 0.617 ± 0.155
4.477ValAsp: 4.477 ± 0.314
5.017ValGlu: 5.017 ± 0.455
2.804ValPhe: 2.804 ± 0.251
3.499ValGly: 3.499 ± 0.374
0.772ValHis: 0.772 ± 0.153
4.194ValIle: 4.194 ± 0.442
5.557ValLys: 5.557 ± 0.366
4.477ValLeu: 4.477 ± 0.352
1.492ValMet: 1.492 ± 0.216
3.885ValAsn: 3.885 ± 0.341
1.518ValPro: 1.518 ± 0.187
2.161ValGln: 2.161 ± 0.23
2.033ValArg: 2.033 ± 0.24
3.962ValSer: 3.962 ± 0.364
4.297ValThr: 4.297 ± 0.306
4.168ValVal: 4.168 ± 0.323
0.978ValTrp: 0.978 ± 0.181
2.907ValTyr: 2.907 ± 0.345
0.0ValXaa: 0.0 ± 0.0
Trp
0.463TrpAla: 0.463 ± 0.111
0.154TrpCys: 0.154 ± 0.058
0.695TrpAsp: 0.695 ± 0.13
0.823TrpGlu: 0.823 ± 0.18
0.463TrpPhe: 0.463 ± 0.143
0.54TrpGly: 0.54 ± 0.109
0.18TrpHis: 0.18 ± 0.065
0.772TrpIle: 0.772 ± 0.142
0.772TrpLys: 0.772 ± 0.101
1.055TrpLeu: 1.055 ± 0.155
0.489TrpMet: 0.489 ± 0.092
1.106TrpAsn: 1.106 ± 0.183
0.026TrpPro: 0.026 ± 0.026
0.309TrpGln: 0.309 ± 0.091
0.437TrpArg: 0.437 ± 0.11
0.72TrpSer: 0.72 ± 0.151
0.746TrpThr: 0.746 ± 0.134
0.695TrpVal: 0.695 ± 0.137
0.103TrpTrp: 0.103 ± 0.051
0.257TrpTyr: 0.257 ± 0.085
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.444TyrAla: 2.444 ± 0.278
0.386TyrCys: 0.386 ± 0.108
3.37TyrAsp: 3.37 ± 0.269
3.293TyrGlu: 3.293 ± 0.344
2.084TyrPhe: 2.084 ± 0.28
2.856TyrGly: 2.856 ± 0.278
0.695TyrHis: 0.695 ± 0.147
3.396TyrIle: 3.396 ± 0.339
3.962TyrLys: 3.962 ± 0.287
3.859TyrLeu: 3.859 ± 0.319
1.003TyrMet: 1.003 ± 0.142
2.547TyrAsn: 2.547 ± 0.253
1.235TyrPro: 1.235 ± 0.217
1.724TyrGln: 1.724 ± 0.166
1.698TyrArg: 1.698 ± 0.198
3.113TyrSer: 3.113 ± 0.296
3.165TyrThr: 3.165 ± 0.314
2.907TyrVal: 2.907 ± 0.262
0.257TyrTrp: 0.257 ± 0.078
2.084TyrTyr: 2.084 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 193 proteins (38868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski