Amino acid dipepetide frequency for Synechococcus phage S-RIM8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.392AlaAla: 6.392 ± 0.561
0.42AlaCys: 0.42 ± 0.092
4.383AlaAsp: 4.383 ± 0.313
4.164AlaGlu: 4.164 ± 0.356
2.612AlaPhe: 2.612 ± 0.25
6.154AlaGly: 6.154 ± 0.448
0.95AlaHis: 0.95 ± 0.14
4.767AlaIle: 4.767 ± 0.346
4.292AlaLys: 4.292 ± 0.473
4.748AlaLeu: 4.748 ± 0.354
1.534AlaMet: 1.534 ± 0.257
4.109AlaAsn: 4.109 ± 0.327
2.557AlaPro: 2.557 ± 0.252
2.758AlaGln: 2.758 ± 0.276
2.648AlaArg: 2.648 ± 0.236
5.168AlaSer: 5.168 ± 0.477
5.296AlaThr: 5.296 ± 0.458
4.529AlaVal: 4.529 ± 0.336
0.676AlaTrp: 0.676 ± 0.146
2.265AlaTyr: 2.265 ± 0.217
0.0AlaXaa: 0.0 ± 0.0
Cys
0.767CysAla: 0.767 ± 0.144
0.073CysCys: 0.073 ± 0.036
0.676CysAsp: 0.676 ± 0.135
0.621CysGlu: 0.621 ± 0.108
0.365CysPhe: 0.365 ± 0.073
0.53CysGly: 0.53 ± 0.112
0.274CysHis: 0.274 ± 0.079
0.584CysIle: 0.584 ± 0.113
0.749CysLys: 0.749 ± 0.139
0.548CysLeu: 0.548 ± 0.111
0.256CysMet: 0.256 ± 0.079
0.475CysAsn: 0.475 ± 0.12
0.31CysPro: 0.31 ± 0.078
0.347CysGln: 0.347 ± 0.086
0.31CysArg: 0.31 ± 0.083
0.493CysSer: 0.493 ± 0.113
0.42CysThr: 0.42 ± 0.11
0.621CysVal: 0.621 ± 0.137
0.164CysTrp: 0.164 ± 0.055
0.237CysTyr: 0.237 ± 0.065
0.0CysXaa: 0.0 ± 0.0
Asp
5.479AspAla: 5.479 ± 0.277
0.676AspCys: 0.676 ± 0.11
4.365AspAsp: 4.365 ± 0.388
4.219AspGlu: 4.219 ± 0.286
3.159AspPhe: 3.159 ± 0.248
5.935AspGly: 5.935 ± 0.544
0.749AspHis: 0.749 ± 0.129
3.926AspIle: 3.926 ± 0.371
3.287AspLys: 3.287 ± 0.289
4.62AspLeu: 4.62 ± 0.281
1.388AspMet: 1.388 ± 0.188
3.616AspAsn: 3.616 ± 0.26
3.251AspPro: 3.251 ± 0.252
2.064AspGln: 2.064 ± 0.202
2.392AspArg: 2.392 ± 0.217
3.945AspSer: 3.945 ± 0.237
4.511AspThr: 4.511 ± 0.383
4.127AspVal: 4.127 ± 0.311
1.023AspTrp: 1.023 ± 0.12
3.433AspTyr: 3.433 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
3.306GluAla: 3.306 ± 0.36
0.621GluCys: 0.621 ± 0.134
4.255GluAsp: 4.255 ± 0.374
4.913GluGlu: 4.913 ± 0.561
3.196GluPhe: 3.196 ± 0.231
4.018GluGly: 4.018 ± 0.299
0.986GluHis: 0.986 ± 0.165
4.328GluIle: 4.328 ± 0.326
3.726GluLys: 3.726 ± 0.514
5.424GluLeu: 5.424 ± 0.396
1.845GluMet: 1.845 ± 0.284
3.196GluAsn: 3.196 ± 0.287
1.954GluPro: 1.954 ± 0.194
2.812GluGln: 2.812 ± 0.259
2.685GluArg: 2.685 ± 0.338
3.726GluSer: 3.726 ± 0.319
3.89GluThr: 3.89 ± 0.289
4.693GluVal: 4.693 ± 0.264
0.931GluTrp: 0.931 ± 0.166
2.812GluTyr: 2.812 ± 0.228
0.0GluXaa: 0.0 ± 0.0
Phe
2.575PheAla: 2.575 ± 0.235
0.584PheCys: 0.584 ± 0.112
3.653PheAsp: 3.653 ± 0.334
2.703PheGlu: 2.703 ± 0.213
1.79PhePhe: 1.79 ± 0.193
3.141PheGly: 3.141 ± 0.229
0.457PheHis: 0.457 ± 0.12
2.685PheIle: 2.685 ± 0.203
2.155PheLys: 2.155 ± 0.255
2.995PheLeu: 2.995 ± 0.231
1.023PheMet: 1.023 ± 0.184
2.721PheAsn: 2.721 ± 0.248
1.771PhePro: 1.771 ± 0.191
1.607PheGln: 1.607 ± 0.157
1.644PheArg: 1.644 ± 0.185
3.105PheSer: 3.105 ± 0.242
3.196PheThr: 3.196 ± 0.297
3.05PheVal: 3.05 ± 0.253
0.329PheTrp: 0.329 ± 0.086
1.863PheTyr: 1.863 ± 0.171
0.0PheXaa: 0.0 ± 0.0
Gly
5.807GlyAla: 5.807 ± 0.511
0.548GlyCys: 0.548 ± 0.117
4.913GlyAsp: 4.913 ± 0.463
4.164GlyGlu: 4.164 ± 0.328
3.214GlyPhe: 3.214 ± 0.221
7.268GlyGly: 7.268 ± 0.786
0.986GlyHis: 0.986 ± 0.151
4.73GlyIle: 4.73 ± 0.35
3.999GlyLys: 3.999 ± 0.47
4.657GlyLeu: 4.657 ± 0.347
1.388GlyMet: 1.388 ± 0.255
4.566GlyAsn: 4.566 ± 0.486
2.1GlyPro: 2.1 ± 0.255
2.904GlyGln: 2.904 ± 0.211
2.94GlyArg: 2.94 ± 0.224
6.136GlySer: 6.136 ± 0.644
6.575GlyThr: 6.575 ± 0.776
5.223GlyVal: 5.223 ± 0.325
1.004GlyTrp: 1.004 ± 0.163
3.269GlyTyr: 3.269 ± 0.325
0.0GlyXaa: 0.0 ± 0.0
His
0.731HisAla: 0.731 ± 0.121
0.256HisCys: 0.256 ± 0.076
0.877HisAsp: 0.877 ± 0.17
0.822HisGlu: 0.822 ± 0.17
0.731HisPhe: 0.731 ± 0.137
0.804HisGly: 0.804 ± 0.127
0.42HisHis: 0.42 ± 0.099
0.749HisIle: 0.749 ± 0.097
0.913HisLys: 0.913 ± 0.187
1.059HisLeu: 1.059 ± 0.137
0.438HisMet: 0.438 ± 0.093
0.822HisAsn: 0.822 ± 0.147
0.986HisPro: 0.986 ± 0.152
0.365HisGln: 0.365 ± 0.084
0.566HisArg: 0.566 ± 0.131
0.858HisSer: 0.858 ± 0.13
1.096HisThr: 1.096 ± 0.155
0.913HisVal: 0.913 ± 0.157
0.219HisTrp: 0.219 ± 0.072
0.804HisTyr: 0.804 ± 0.153
0.0HisXaa: 0.0 ± 0.0
Ile
4.62IleAla: 4.62 ± 0.264
0.475IleCys: 0.475 ± 0.099
5.187IleAsp: 5.187 ± 0.393
4.602IleGlu: 4.602 ± 0.32
2.465IlePhe: 2.465 ± 0.194
4.493IleGly: 4.493 ± 0.37
0.694IleHis: 0.694 ± 0.128
4.146IleIle: 4.146 ± 0.367
3.981IleLys: 3.981 ± 0.348
4.346IleLeu: 4.346 ± 0.352
0.895IleMet: 0.895 ± 0.158
3.817IleAsn: 3.817 ± 0.271
2.557IlePro: 2.557 ± 0.24
2.703IleGln: 2.703 ± 0.243
2.703IleArg: 2.703 ± 0.219
4.639IleSer: 4.639 ± 0.533
5.552IleThr: 5.552 ± 0.567
4.456IleVal: 4.456 ± 0.293
0.694IleTrp: 0.694 ± 0.116
2.338IleTyr: 2.338 ± 0.246
0.0IleXaa: 0.0 ± 0.0
Lys
3.47LysAla: 3.47 ± 0.404
0.53LysCys: 0.53 ± 0.114
3.123LysAsp: 3.123 ± 0.348
3.926LysGlu: 3.926 ± 0.528
2.465LysPhe: 2.465 ± 0.224
3.141LysGly: 3.141 ± 0.344
0.968LysHis: 0.968 ± 0.175
3.999LysIle: 3.999 ± 0.294
5.022LysLys: 5.022 ± 0.788
4.767LysLeu: 4.767 ± 0.31
1.297LysMet: 1.297 ± 0.238
3.05LysAsn: 3.05 ± 0.243
1.516LysPro: 1.516 ± 0.196
2.1LysGln: 2.1 ± 0.311
2.557LysArg: 2.557 ± 0.351
4.365LysSer: 4.365 ± 0.443
3.306LysThr: 3.306 ± 0.281
3.945LysVal: 3.945 ± 0.295
0.731LysTrp: 0.731 ± 0.179
2.831LysTyr: 2.831 ± 0.414
0.0LysXaa: 0.0 ± 0.0
Leu
4.493LeuAla: 4.493 ± 0.399
0.767LeuCys: 0.767 ± 0.154
5.881LeuAsp: 5.881 ± 0.336
4.62LeuGlu: 4.62 ± 0.329
2.812LeuPhe: 2.812 ± 0.235
4.967LeuGly: 4.967 ± 0.392
1.242LeuHis: 1.242 ± 0.214
4.383LeuIle: 4.383 ± 0.321
4.967LeuLys: 4.967 ± 0.398
5.223LeuLeu: 5.223 ± 0.362
1.151LeuMet: 1.151 ± 0.208
4.657LeuAsn: 4.657 ± 0.245
3.159LeuPro: 3.159 ± 0.287
3.232LeuGln: 3.232 ± 0.254
3.251LeuArg: 3.251 ± 0.259
4.383LeuSer: 4.383 ± 0.294
5.424LeuThr: 5.424 ± 0.435
4.401LeuVal: 4.401 ± 0.263
0.603LeuTrp: 0.603 ± 0.124
3.159LeuTyr: 3.159 ± 0.225
0.0LeuXaa: 0.0 ± 0.0
Met
1.735MetAla: 1.735 ± 0.251
0.183MetCys: 0.183 ± 0.066
1.023MetAsp: 1.023 ± 0.205
1.096MetGlu: 1.096 ± 0.214
0.84MetPhe: 0.84 ± 0.155
1.077MetGly: 1.077 ± 0.217
0.402MetHis: 0.402 ± 0.113
1.187MetIle: 1.187 ± 0.224
1.278MetLys: 1.278 ± 0.24
1.625MetLeu: 1.625 ± 0.264
0.53MetMet: 0.53 ± 0.148
1.077MetAsn: 1.077 ± 0.171
0.931MetPro: 0.931 ± 0.184
1.004MetGln: 1.004 ± 0.169
1.059MetArg: 1.059 ± 0.205
1.498MetSer: 1.498 ± 0.264
1.479MetThr: 1.479 ± 0.243
0.913MetVal: 0.913 ± 0.135
0.219MetTrp: 0.219 ± 0.07
0.712MetTyr: 0.712 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
4.31AsnAla: 4.31 ± 0.374
0.438AsnCys: 0.438 ± 0.087
3.397AsnAsp: 3.397 ± 0.219
3.324AsnGlu: 3.324 ± 0.273
2.429AsnPhe: 2.429 ± 0.202
4.639AsnGly: 4.639 ± 0.52
0.767AsnHis: 0.767 ± 0.116
3.926AsnIle: 3.926 ± 0.48
2.885AsnLys: 2.885 ± 0.25
4.785AsnLeu: 4.785 ± 0.309
0.858AsnMet: 0.858 ± 0.188
3.579AsnAsn: 3.579 ± 0.316
2.776AsnPro: 2.776 ± 0.197
2.027AsnGln: 2.027 ± 0.207
2.301AsnArg: 2.301 ± 0.195
3.835AsnSer: 3.835 ± 0.36
4.237AsnThr: 4.237 ± 0.501
4.219AsnVal: 4.219 ± 0.394
0.584AsnTrp: 0.584 ± 0.116
2.538AsnTyr: 2.538 ± 0.206
0.0AsnXaa: 0.0 ± 0.0
Pro
2.831ProAla: 2.831 ± 0.211
0.31ProCys: 0.31 ± 0.088
2.758ProAsp: 2.758 ± 0.273
2.685ProGlu: 2.685 ± 0.214
1.552ProPhe: 1.552 ± 0.167
3.269ProGly: 3.269 ± 0.323
0.584ProHis: 0.584 ± 0.095
2.392ProIle: 2.392 ± 0.237
2.118ProLys: 2.118 ± 0.264
2.027ProLeu: 2.027 ± 0.2
0.584ProMet: 0.584 ± 0.142
2.538ProAsn: 2.538 ± 0.236
1.753ProPro: 1.753 ± 0.214
1.242ProGln: 1.242 ± 0.138
1.552ProArg: 1.552 ± 0.152
2.739ProSer: 2.739 ± 0.225
2.776ProThr: 2.776 ± 0.209
2.557ProVal: 2.557 ± 0.242
0.475ProTrp: 0.475 ± 0.118
1.863ProTyr: 1.863 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
2.082GlnAla: 2.082 ± 0.214
0.31GlnCys: 0.31 ± 0.075
2.356GlnAsp: 2.356 ± 0.231
2.666GlnGlu: 2.666 ± 0.29
1.845GlnPhe: 1.845 ± 0.242
2.283GlnGly: 2.283 ± 0.166
0.785GlnHis: 0.785 ± 0.153
2.977GlnIle: 2.977 ± 0.2
2.246GlnLys: 2.246 ± 0.236
3.415GlnLeu: 3.415 ± 0.255
0.822GlnMet: 0.822 ± 0.163
2.118GlnAsn: 2.118 ± 0.197
1.242GlnPro: 1.242 ± 0.155
1.735GlnGln: 1.735 ± 0.227
1.899GlnArg: 1.899 ± 0.174
2.52GlnSer: 2.52 ± 0.18
2.338GlnThr: 2.338 ± 0.227
2.831GlnVal: 2.831 ± 0.222
0.438GlnTrp: 0.438 ± 0.104
1.845GlnTyr: 1.845 ± 0.207
0.0GlnXaa: 0.0 ± 0.0
Arg
2.812ArgAla: 2.812 ± 0.307
0.347ArgCys: 0.347 ± 0.083
2.356ArgAsp: 2.356 ± 0.185
2.758ArgGlu: 2.758 ± 0.41
1.808ArgPhe: 1.808 ± 0.167
2.794ArgGly: 2.794 ± 0.266
0.749ArgHis: 0.749 ± 0.134
3.013ArgIle: 3.013 ± 0.306
2.356ArgLys: 2.356 ± 0.332
3.196ArgLeu: 3.196 ± 0.229
1.023ArgMet: 1.023 ± 0.226
2.192ArgAsn: 2.192 ± 0.186
1.315ArgPro: 1.315 ± 0.145
1.753ArgGln: 1.753 ± 0.177
2.064ArgArg: 2.064 ± 0.307
2.173ArgSer: 2.173 ± 0.265
2.593ArgThr: 2.593 ± 0.252
3.141ArgVal: 3.141 ± 0.255
0.493ArgTrp: 0.493 ± 0.103
2.301ArgTyr: 2.301 ± 0.206
0.0ArgXaa: 0.0 ± 0.0
Ser
5.314SerAla: 5.314 ± 0.376
0.475SerCys: 0.475 ± 0.129
4.146SerAsp: 4.146 ± 0.245
3.689SerGlu: 3.689 ± 0.28
3.141SerPhe: 3.141 ± 0.248
6.995SerGly: 6.995 ± 0.508
0.84SerHis: 0.84 ± 0.106
5.04SerIle: 5.04 ± 0.493
3.269SerLys: 3.269 ± 0.278
4.675SerLeu: 4.675 ± 0.258
1.333SerMet: 1.333 ± 0.211
3.634SerAsn: 3.634 ± 0.276
2.429SerPro: 2.429 ± 0.274
2.685SerGln: 2.685 ± 0.277
2.484SerArg: 2.484 ± 0.187
5.844SerSer: 5.844 ± 0.466
5.314SerThr: 5.314 ± 0.457
4.931SerVal: 4.931 ± 0.396
0.584SerTrp: 0.584 ± 0.097
2.849SerTyr: 2.849 ± 0.205
0.0SerXaa: 0.0 ± 0.0
Thr
5.917ThrAla: 5.917 ± 0.601
0.53ThrCys: 0.53 ± 0.119
3.671ThrAsp: 3.671 ± 0.324
4.146ThrGlu: 4.146 ± 0.325
3.525ThrPhe: 3.525 ± 0.328
6.648ThrGly: 6.648 ± 0.878
0.804ThrHis: 0.804 ± 0.158
5.059ThrIle: 5.059 ± 0.507
3.452ThrLys: 3.452 ± 0.263
6.027ThrLeu: 6.027 ± 0.481
1.041ThrMet: 1.041 ± 0.188
3.853ThrAsn: 3.853 ± 0.551
3.068ThrPro: 3.068 ± 0.234
2.739ThrGln: 2.739 ± 0.198
2.666ThrArg: 2.666 ± 0.237
5.57ThrSer: 5.57 ± 0.549
5.643ThrThr: 5.643 ± 0.695
5.515ThrVal: 5.515 ± 0.501
0.84ThrTrp: 0.84 ± 0.155
2.776ThrTyr: 2.776 ± 0.226
0.0ThrXaa: 0.0 ± 0.0
Val
4.73ValAla: 4.73 ± 0.314
0.603ValCys: 0.603 ± 0.11
5.095ValAsp: 5.095 ± 0.404
4.493ValGlu: 4.493 ± 0.276
2.831ValPhe: 2.831 ± 0.229
4.949ValGly: 4.949 ± 0.411
0.749ValHis: 0.749 ± 0.127
4.127ValIle: 4.127 ± 0.31
3.306ValLys: 3.306 ± 0.288
4.675ValLeu: 4.675 ± 0.332
1.187ValMet: 1.187 ± 0.193
4.273ValAsn: 4.273 ± 0.36
3.013ValPro: 3.013 ± 0.209
2.447ValGln: 2.447 ± 0.203
2.648ValArg: 2.648 ± 0.206
5.15ValSer: 5.15 ± 0.302
6.1ValThr: 6.1 ± 0.574
4.712ValVal: 4.712 ± 0.564
0.603ValTrp: 0.603 ± 0.084
2.776ValTyr: 2.776 ± 0.242
0.0ValXaa: 0.0 ± 0.0
Trp
0.767TrpAla: 0.767 ± 0.137
0.164TrpCys: 0.164 ± 0.052
0.804TrpAsp: 0.804 ± 0.14
0.785TrpGlu: 0.785 ± 0.162
0.493TrpPhe: 0.493 ± 0.118
0.731TrpGly: 0.731 ± 0.118
0.347TrpHis: 0.347 ± 0.104
0.384TrpIle: 0.384 ± 0.071
0.785TrpLys: 0.785 ± 0.158
0.731TrpLeu: 0.731 ± 0.144
0.42TrpMet: 0.42 ± 0.104
0.84TrpAsn: 0.84 ± 0.128
0.146TrpPro: 0.146 ± 0.049
0.53TrpGln: 0.53 ± 0.098
0.438TrpArg: 0.438 ± 0.088
0.712TrpSer: 0.712 ± 0.099
0.877TrpThr: 0.877 ± 0.127
0.822TrpVal: 0.822 ± 0.103
0.146TrpTrp: 0.146 ± 0.053
0.31TrpTyr: 0.31 ± 0.077
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.283TyrAla: 2.283 ± 0.192
0.511TyrCys: 0.511 ± 0.112
3.251TyrAsp: 3.251 ± 0.261
2.922TyrGlu: 2.922 ± 0.309
1.771TyrPhe: 1.771 ± 0.218
2.557TyrGly: 2.557 ± 0.219
0.676TyrHis: 0.676 ± 0.135
2.977TyrIle: 2.977 ± 0.219
2.319TyrLys: 2.319 ± 0.193
3.232TyrLeu: 3.232 ± 0.243
0.84TyrMet: 0.84 ± 0.18
2.721TyrAsn: 2.721 ± 0.223
1.771TyrPro: 1.771 ± 0.17
1.698TyrGln: 1.698 ± 0.171
2.429TyrArg: 2.429 ± 0.258
2.812TyrSer: 2.812 ± 0.265
2.867TyrThr: 2.867 ± 0.312
2.867TyrVal: 2.867 ± 0.234
0.475TyrTrp: 0.475 ± 0.116
2.009TyrTyr: 2.009 ± 0.207
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 222 proteins (54758 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski