Candidatus Sedimenticola endophacoides
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1190 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A657PQL3|A0A657PQL3_9GAMM Cell division protein ZapE (Fragment) OS=Candidatus Sedimenticola endophacoides OX=2548426 GN=B0D84_00060 PE=4 SV=1
MM1 pKa = 7.52 NDD3 pKa = 3.4 AVTDD7 pKa = 3.93 APLEE11 pKa = 4.17 FTPSAAAKK19 pKa = 9.59 VAALIQEE26 pKa = 4.35 EE27 pKa = 4.87 GNPGLMLRR35 pKa = 11.84 VYY37 pKa = 9.87 IQGGGCSGFQYY48 pKa = 10.82 GFAFDD53 pKa = 4.38 EE54 pKa = 4.54 EE55 pKa = 4.6 EE56 pKa = 4.65 KK57 pKa = 11.14 EE58 pKa = 3.71 GDD60 pKa = 3.4 TRR62 pKa = 11.84 VEE64 pKa = 3.91 RR65 pKa = 11.84 DD66 pKa = 3.39 GVTLLVDD73 pKa = 4.1 PMSMQYY79 pKa = 11.63 LMGAEE84 pKa = 3.76 VDD86 pKa = 4.08 YY87 pKa = 11.87 VEE89 pKa = 5.28 GLQGSQFVIRR99 pKa = 11.84 NPNASTTCGCGSSFSVV115 pKa = 3.54
Molecular weight: 12.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.732
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.745
Grimsley 3.656
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.151
Thurlkill 3.77
EMBOSS 3.795
Sillero 4.024
Patrickios 1.888
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|A0A657Q7Q1|A0A657Q7Q1_9GAMM Uncharacterized protein OS=Candidatus Sedimenticola endophacoides OX=2548426 GN=B0D84_04205 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 9.97 RR12 pKa = 11.84 ARR14 pKa = 11.84 AHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 NGRR28 pKa = 11.84 KK29 pKa = 9.4 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.33 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LTPP44 pKa = 3.82
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1190
0
1190
301048
28
1405
253.0
27.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.195 ± 0.086
1.057 ± 0.033
5.438 ± 0.056
6.94 ± 0.072
3.558 ± 0.049
8.61 ± 0.068
2.434 ± 0.036
5.336 ± 0.042
2.975 ± 0.054
11.319 ± 0.101
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.559 ± 0.038
2.743 ± 0.042
4.789 ± 0.049
3.759 ± 0.052
7.832 ± 0.081
5.167 ± 0.052
4.572 ± 0.045
6.931 ± 0.061
1.185 ± 0.025
2.601 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here