Parabacteroides sp. AF48-14
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3786 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A415ZP48|A0A415ZP48_9BACT Uncharacterized protein OS=Parabacteroides sp. AF48-14 OX=2292052 GN=DW083_19970 PE=4 SV=1
MM1 pKa = 7.28 EE2 pKa = 5.61 AVTLSEE8 pKa = 3.54 ARR10 pKa = 11.84 VYY12 pKa = 10.88 VGTYY16 pKa = 9.56 NKK18 pKa = 10.08 YY19 pKa = 10.6 NNGSLFGKK27 pKa = 9.26 WLDD30 pKa = 3.72 LSDD33 pKa = 5.06 YY34 pKa = 10.69 SDD36 pKa = 3.45 KK37 pKa = 11.77 DD38 pKa = 3.52 EE39 pKa = 4.48 FLEE42 pKa = 4.84 ACRR45 pKa = 11.84 EE46 pKa = 4.08 LHH48 pKa = 7.34 KK49 pKa = 10.91 DD50 pKa = 3.54 DD51 pKa = 4.58 QDD53 pKa = 3.96 PEE55 pKa = 5.08 FMFQDD60 pKa = 3.9 YY61 pKa = 11.28 EE62 pKa = 4.42 NIPEE66 pKa = 4.13 ALISEE71 pKa = 4.48 SWLSEE76 pKa = 3.76 KK77 pKa = 10.49 FFEE80 pKa = 5.55 LRR82 pKa = 11.84 DD83 pKa = 4.29 AIEE86 pKa = 4.48 KK87 pKa = 10.62 LSEE90 pKa = 4.15 TEE92 pKa = 3.79 QEE94 pKa = 5.56 AFFVWCDD101 pKa = 3.32 HH102 pKa = 6.55 HH103 pKa = 8.25 NSDD106 pKa = 5.34 ISEE109 pKa = 4.05 EE110 pKa = 4.1 DD111 pKa = 3.42 ADD113 pKa = 4.39 DD114 pKa = 5.21 LISSFEE120 pKa = 4.1 DD121 pKa = 4.17 EE122 pKa = 4.47 YY123 pKa = 11.31 QGEE126 pKa = 4.44 YY127 pKa = 10.22 KK128 pKa = 10.73 DD129 pKa = 4.14 EE130 pKa = 3.79 EE131 pKa = 5.05 DD132 pKa = 3.37 YY133 pKa = 11.08 AYY135 pKa = 10.08 EE136 pKa = 4.47 IVEE139 pKa = 3.94 QCYY142 pKa = 10.53 DD143 pKa = 3.45 LPEE146 pKa = 4.03 FAKK149 pKa = 10.18 TYY151 pKa = 10.45 FDD153 pKa = 3.79 YY154 pKa = 11.39 SAFARR159 pKa = 11.84 DD160 pKa = 5.17 LFITDD165 pKa = 2.97 YY166 pKa = 11.18 WMDD169 pKa = 3.15 NGFVFRR175 pKa = 11.84 CAA177 pKa = 3.58
Molecular weight: 21.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.643
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.554
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.732
Sillero 3.961
Patrickios 0.757
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.853
Protein with the highest isoelectric point:
>tr|A0A416A6L9|A0A416A6L9_9BACT Beta-galactosidase OS=Parabacteroides sp. AF48-14 OX=2292052 GN=DW083_10180 PE=3 SV=1
MM1 pKa = 7.6 FEE3 pKa = 5.7 LGPLWGFEE11 pKa = 3.81 KK12 pKa = 10.59 HH13 pKa = 5.71 VLYY16 pKa = 11.1 NKK18 pKa = 9.68 LRR20 pKa = 11.84 QIFLQLTNNPIPLFVVYY37 pKa = 10.4 SFYY40 pKa = 10.75 INKK43 pKa = 8.46 KK44 pKa = 7.99 TMTTNRR50 pKa = 11.84 TKK52 pKa = 10.55 RR53 pKa = 11.84 RR54 pKa = 11.84 PSEE57 pKa = 4.03 VYY59 pKa = 10.3 LFRR62 pKa = 11.84 TIDD65 pKa = 3.19 TLIFRR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 SAGRR76 pKa = 11.84 DD77 pKa = 3.19 ATADD81 pKa = 4.0 LYY83 pKa = 10.97 RR84 pKa = 11.84 AAGNWFRR91 pKa = 11.84 KK92 pKa = 8.7 FRR94 pKa = 11.84 NGRR97 pKa = 11.84 DD98 pKa = 3.28 LCLLGMTPSLVDD110 pKa = 4.14 ALSLSCRR117 pKa = 11.84 PGKK120 pKa = 10.32 SS121 pKa = 2.84
Molecular weight: 14.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.867
IPC_protein 10.73
Toseland 10.745
ProMoST 10.482
Dawson 10.847
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.052
Grimsley 10.906
Solomon 10.979
Lehninger 10.935
Nozaki 10.73
DTASelect 10.584
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.789
Patrickios 10.774
IPC_peptide 10.979
IPC2_peptide 9.663
IPC2.peptide.svr19 8.384
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3786
0
3786
1319893
22
2158
348.6
39.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.22 ± 0.048
1.15 ± 0.015
5.677 ± 0.028
6.544 ± 0.041
4.589 ± 0.026
6.901 ± 0.037
1.731 ± 0.017
6.791 ± 0.035
6.617 ± 0.032
9.173 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.628 ± 0.019
5.063 ± 0.032
3.805 ± 0.024
3.402 ± 0.024
4.623 ± 0.037
6.139 ± 0.026
5.703 ± 0.042
6.53 ± 0.036
1.256 ± 0.016
4.457 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here