Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales; Acidithiobacillaceae; Acidithiobacillus; Acidithiobacillus ferrooxidans

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7J905|B7J905_ACIF2 Uncharacterized protein OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455) OX=243159 GN=AFE_2812 PE=4 SV=1
MM1 pKa = 7.08QEE3 pKa = 3.79KK4 pKa = 10.57DD5 pKa = 3.33FTRR8 pKa = 11.84IITAMRR14 pKa = 11.84ATGACSLRR22 pKa = 11.84AEE24 pKa = 4.3YY25 pKa = 10.16HH26 pKa = 5.97GSGDD30 pKa = 3.88SMDD33 pKa = 3.94SFTMSFADD41 pKa = 3.95GQGNDD46 pKa = 3.18LSLPKK51 pKa = 10.15EE52 pKa = 4.19VASDD56 pKa = 3.63IQNQLYY62 pKa = 10.44DD63 pKa = 4.47LLVGDD68 pKa = 4.7HH69 pKa = 6.78EE70 pKa = 4.48GWEE73 pKa = 4.27NNEE76 pKa = 3.74GGYY79 pKa = 11.06GEE81 pKa = 4.9ISLDD85 pKa = 3.94LDD87 pKa = 4.22DD88 pKa = 6.1GVPTMDD94 pKa = 3.71WDD96 pKa = 3.78HH97 pKa = 6.73YY98 pKa = 11.2DD99 pKa = 3.74YY100 pKa = 11.73VVDD103 pKa = 4.12TSLTSHH109 pKa = 6.61SFGDD113 pKa = 4.02DD114 pKa = 2.98

Molecular weight: 12.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7J3M0|B7J3M0_ACIF2 tRNA N6-adenosine threonylcarbamoyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455) OX=243159 GN=tsaD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVVHH11 pKa = 6.89RR12 pKa = 11.84KK13 pKa = 7.38RR14 pKa = 11.84THH16 pKa = 5.56GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29SGRR28 pKa = 11.84LVLKK32 pKa = 10.26RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84QRR41 pKa = 11.84LCPP44 pKa = 3.78

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3120

0

3120

882239

30

3484

282.8

31.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.226 ± 0.06

1.014 ± 0.017

5.06 ± 0.038

5.354 ± 0.055

3.56 ± 0.031

8.276 ± 0.058

2.708 ± 0.025

5.237 ± 0.031

3.04 ± 0.037

10.944 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.023

2.805 ± 0.043

5.358 ± 0.031

4.211 ± 0.033

7.035 ± 0.058

5.392 ± 0.041

4.96 ± 0.039

7.033 ± 0.04

1.604 ± 0.024

2.626 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski