Hydrogenophaga sp. T4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga; unclassified Hydrogenophaga

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7WWU5|W7WWU5_9BURK Uncharacterized protein OS=Hydrogenophaga sp. T4 OX=1437444 GN=Y695_00873 PE=4 SV=1
MM1 pKa = 7.75CPTTLVTGFNAADD14 pKa = 3.5QYY16 pKa = 11.67LEE18 pKa = 4.05SHH20 pKa = 6.85LASPEE25 pKa = 4.17TVLATARR32 pKa = 11.84YY33 pKa = 8.48EE34 pKa = 3.99RR35 pKa = 11.84FEE37 pKa = 4.21ATFSGAGDD45 pKa = 3.74TLSAALCALIAGGSDD60 pKa = 3.37LQSACAEE67 pKa = 3.93ALTYY71 pKa = 10.72LDD73 pKa = 3.41QCLDD77 pKa = 3.39AGFQPGMGHH86 pKa = 7.25AVPDD90 pKa = 3.8RR91 pKa = 11.84LFWAHH96 pKa = 7.43DD97 pKa = 3.72DD98 pKa = 4.34DD99 pKa = 7.17GEE101 pKa = 5.45DD102 pKa = 3.54EE103 pKa = 5.57AGTADD108 pKa = 3.61SSSDD112 pKa = 3.48STLDD116 pKa = 3.97ADD118 pKa = 5.84DD119 pKa = 5.23FPLDD123 pKa = 3.9TTRR126 pKa = 11.84HH127 pKa = 4.6

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7X2K7|W7X2K7_9BURK Uric acid transporter UacT OS=Hydrogenophaga sp. T4 OX=1437444 GN=uacT PE=4 SV=1
MM1 pKa = 7.42AFSVRR6 pKa = 11.84AKK8 pKa = 9.3WRR10 pKa = 11.84TRR12 pKa = 11.84KK13 pKa = 10.23NSMASTHH20 pKa = 6.37RR21 pKa = 11.84ASTPHH26 pKa = 6.94NISTWRR32 pKa = 11.84TQKK35 pKa = 10.36NGRR38 pKa = 11.84WSSSRR43 pKa = 11.84SRR45 pKa = 11.84SVPPPNAVRR54 pKa = 11.84KK55 pKa = 6.91PTTHH59 pKa = 6.76TPTASSRR66 pKa = 11.84LRR68 pKa = 11.84APSMMPASAKK78 pKa = 10.27AAVPVSSTSRR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84VSPQNN95 pKa = 3.28

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4866

0

4866

880246

29

1659

180.9

19.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.016 ± 0.045

1.262 ± 0.018

4.981 ± 0.034

5.206 ± 0.037

3.743 ± 0.029

7.913 ± 0.039

2.504 ± 0.029

4.505 ± 0.034

3.942 ± 0.037

9.948 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.086 ± 0.028

2.862 ± 0.024

5.186 ± 0.036

4.01 ± 0.034

6.689 ± 0.046

6.196 ± 0.049

5.506 ± 0.034

7.697 ± 0.037

1.575 ± 0.021

2.175 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski