Sanxia Water Strider Virus 1
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5KS14|A0A0B5KS14_9VIRU Glycoprotein G2 OS=Sanxia Water Strider Virus 1 OX=1608060 GN=G PE=4 SV=1
MM1 pKa = 7.63 AAPAPFNVITSYY13 pKa = 9.26 EE14 pKa = 4.18 TASVSVNALRR24 pKa = 11.84 TVSVNITSCPTFLNAFPDD42 pKa = 3.37 NYY44 pKa = 10.96 NNYY47 pKa = 7.96 IQKK50 pKa = 9.93 INAMRR55 pKa = 11.84 AASPKK60 pKa = 9.72 EE61 pKa = 3.93 KK62 pKa = 10.41 NAVVADD68 pKa = 4.01 MVTEE72 pKa = 4.37 TLRR75 pKa = 11.84 DD76 pKa = 3.44 ACPAVAQFYY85 pKa = 10.74 CLGQGFVTRR94 pKa = 11.84 SRR96 pKa = 11.84 AFFSTDD102 pKa = 2.75 PSVQVLNYY110 pKa = 9.77 DD111 pKa = 3.72 AYY113 pKa = 9.94 IATGTQVTDD122 pKa = 3.64 PKK124 pKa = 11.08 EE125 pKa = 3.9 IDD127 pKa = 4.35 DD128 pKa = 4.37 YY129 pKa = 10.86 NHH131 pKa = 6.81 CVDD134 pKa = 3.84 SYY136 pKa = 9.91 RR137 pKa = 11.84 TYY139 pKa = 10.21 MVNGGFALNNEE150 pKa = 4.32 LATLRR155 pKa = 11.84 GAVKK159 pKa = 9.28 NTINASGNLVPLQVMLRR176 pKa = 11.84 EE177 pKa = 4.34 MIEE180 pKa = 3.76 KK181 pKa = 10.32 RR182 pKa = 11.84 RR183 pKa = 11.84 FNLGGTSAGAEE194 pKa = 4.05 HH195 pKa = 6.35 LRR197 pKa = 11.84 EE198 pKa = 4.0 VRR200 pKa = 11.84 EE201 pKa = 4.11 ALSGDD206 pKa = 3.96 LDD208 pKa = 3.73 QKK210 pKa = 11.01 IIFPFSKK217 pKa = 10.23 SEE219 pKa = 3.83 RR220 pKa = 11.84 KK221 pKa = 9.4 AVGFSFSNQINNRR234 pKa = 11.84 AEE236 pKa = 3.92 RR237 pKa = 11.84 NEE239 pKa = 3.86 GGRR242 pKa = 11.84 CLLASTAVAKK252 pKa = 8.2 DD253 pKa = 3.54 TQLEE257 pKa = 4.09 GDD259 pKa = 4.41 LLNTITRR266 pKa = 11.84 KK267 pKa = 8.92 FHH269 pKa = 7.38 AMPQDD274 pKa = 3.31 HH275 pKa = 7.01 VNYY278 pKa = 10.24 QLFDD282 pKa = 4.94 RR283 pKa = 11.84 IIRR286 pKa = 11.84 EE287 pKa = 4.09 ANAILDD293 pKa = 4.16 PEE295 pKa = 4.76 DD296 pKa = 3.47 VDD298 pKa = 4.14 AADD301 pKa = 3.93 GAGFLSQRR309 pKa = 11.84 SNIDD313 pKa = 3.55 PLWLTFYY320 pKa = 10.8 FAFKK324 pKa = 10.42 VIKK327 pKa = 10.54 LSVADD332 pKa = 4.51 FYY334 pKa = 11.39 KK335 pKa = 9.63 WQRR338 pKa = 11.84 AIYY341 pKa = 7.33 ATCRR345 pKa = 11.84 KK346 pKa = 9.36 IRR348 pKa = 11.84 GKK350 pKa = 10.54 EE351 pKa = 3.74 NIRR354 pKa = 11.84 AVLATMLDD362 pKa = 3.67 LPGVQDD368 pKa = 3.7 TLGKK372 pKa = 9.47 MSQSTEE378 pKa = 3.18 IHH380 pKa = 5.25 TPPWVMHH387 pKa = 6.13 FSRR390 pKa = 11.84 LYY392 pKa = 10.67 DD393 pKa = 3.3 IAAVICPCDD402 pKa = 3.78 AYY404 pKa = 11.33 NVAATIQTPAISWRR418 pKa = 11.84 YY419 pKa = 9.69 LFTVNPAVKK428 pKa = 10.38 GFISYY433 pKa = 7.81 LTTLNAVEE441 pKa = 4.27 KK442 pKa = 9.45 TADD445 pKa = 3.58 EE446 pKa = 4.2 TSEE449 pKa = 3.66 IHH451 pKa = 5.75 QSEE454 pKa = 4.6 RR455 pKa = 11.84 MLQQFILGRR464 pKa = 11.84 NSPLANTNHH473 pKa = 6.04 LTGNALDD480 pKa = 3.82 VTIVGG485 pKa = 4.02
Molecular weight: 53.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.049
IPC2_protein 6.084
IPC_protein 6.173
Toseland 6.376
ProMoST 6.561
Dawson 6.491
Bjellqvist 6.465
Wikipedia 6.504
Rodwell 6.478
Grimsley 6.491
Solomon 6.504
Lehninger 6.491
Nozaki 6.766
DTASelect 6.927
Thurlkill 6.942
EMBOSS 6.927
Sillero 6.883
Patrickios 4.126
IPC_peptide 6.517
IPC2_peptide 6.737
IPC2.peptide.svr19 6.7
Protein with the highest isoelectric point:
>tr|A0A0B5KS14|A0A0B5KS14_9VIRU Glycoprotein G2 OS=Sanxia Water Strider Virus 1 OX=1608060 GN=G PE=4 SV=1
MM1 pKa = 7.05 MLSYY5 pKa = 10.79 CATRR9 pKa = 11.84 KK10 pKa = 9.76 VIPLWTVYY18 pKa = 10.37 CYY20 pKa = 10.94 LILATVNTVDD30 pKa = 4.64 CNLEE34 pKa = 4.03 GLSQNDD40 pKa = 3.59 LPISDD45 pKa = 4.72 RR46 pKa = 11.84 LQIVQSTASDD56 pKa = 3.83 PSNGALIKK64 pKa = 10.7 YY65 pKa = 7.37 MYY67 pKa = 9.93 HH68 pKa = 6.16 GRR70 pKa = 11.84 QFDD73 pKa = 4.09 TIHH76 pKa = 6.73 FNFLMSSSEE85 pKa = 3.99 FTLLKK90 pKa = 10.31 PKK92 pKa = 10.78 NSTCAILKK100 pKa = 10.4 GPLIDD105 pKa = 5.47 KK106 pKa = 10.54 EE107 pKa = 4.26 DD108 pKa = 4.02 FKK110 pKa = 11.63 HH111 pKa = 5.07 FTFYY115 pKa = 11.14 SATCTIKK122 pKa = 10.34 RR123 pKa = 11.84 YY124 pKa = 9.87 EE125 pKa = 4.48 DD126 pKa = 3.41 FTVSVVGEE134 pKa = 4.11 DD135 pKa = 3.45 KK136 pKa = 10.32 KK137 pKa = 9.59 TSLSFNDD144 pKa = 4.57 DD145 pKa = 3.28 LSTTCHH151 pKa = 6.68 SSLTSVWQFQLTTSNEE167 pKa = 3.94 HH168 pKa = 6.46 LSINNFLLCKK178 pKa = 10.38 NGSLFNIQQSNILIHH193 pKa = 6.68 EE194 pKa = 4.43 CTTLYY199 pKa = 10.57 LIKK202 pKa = 10.56 NLQPCLEE209 pKa = 4.71 PLSQGKK215 pKa = 8.9 KK216 pKa = 8.33 VCSISSWKK224 pKa = 10.66 SEE226 pKa = 3.89 GEE228 pKa = 3.87 DD229 pKa = 4.44 LKK231 pKa = 11.53 LHH233 pKa = 6.64 LLNSPSGSINIITDD247 pKa = 3.0 TDD249 pKa = 4.03 KK250 pKa = 11.01 IQRR253 pKa = 11.84 FCNSACEE260 pKa = 4.21 FKK262 pKa = 11.06 VRR264 pKa = 11.84 AINYY268 pKa = 9.3 ISLTCTDD275 pKa = 3.81 GSQDD279 pKa = 3.16 FKK281 pKa = 11.14 QVHH284 pKa = 5.74 KK285 pKa = 10.27 EE286 pKa = 4.06 VVEE289 pKa = 4.13 SCPFITSSKK298 pKa = 9.94 IGYY301 pKa = 8.58 LALTFCRR308 pKa = 11.84 LSTHH312 pKa = 7.13 PLLLWFLVLWIILGYY327 pKa = 8.29 PICILFSWISTKK339 pKa = 10.52 LYY341 pKa = 10.04 RR342 pKa = 11.84 LSIYY346 pKa = 8.55 VFRR349 pKa = 11.84 YY350 pKa = 10.09 VSIKK354 pKa = 10.47 KK355 pKa = 10.08 CINHH359 pKa = 6.82 NICYY363 pKa = 9.93 DD364 pKa = 3.66 CNSEE368 pKa = 4.24 YY369 pKa = 11.09 KK370 pKa = 10.16 FDD372 pKa = 4.14 NLIEE376 pKa = 4.03 LHH378 pKa = 5.42 STCINGICPYY388 pKa = 9.93 CRR390 pKa = 11.84 VGVGEE395 pKa = 4.0 NVIDD399 pKa = 3.87 HH400 pKa = 6.19 VKK402 pKa = 9.33 SCKK405 pKa = 10.05 SKK407 pKa = 8.68 VYY409 pKa = 10.01 RR410 pKa = 11.84 YY411 pKa = 10.03 KK412 pKa = 10.82 EE413 pKa = 3.88 QLTLLQSNSKK423 pKa = 10.12 LEE425 pKa = 4.14 HH426 pKa = 5.92 GAALTVSIIRR436 pKa = 11.84 SKK438 pKa = 10.39 LTIITIYY445 pKa = 10.62 ILVLLFAMVPMTNSEE460 pKa = 4.11 HH461 pKa = 6.98 FIPNITDD468 pKa = 3.2 QYY470 pKa = 9.11 GHH472 pKa = 6.51 VIEE475 pKa = 5.4 FNTTAEE481 pKa = 4.78 DD482 pKa = 3.81 YY483 pKa = 11.38 LSLFKK488 pKa = 10.84 STDD491 pKa = 3.4 SLLKK495 pKa = 9.62 PAQGNDD501 pKa = 3.71 YY502 pKa = 10.1 YY503 pKa = 11.72 SKK505 pKa = 10.7 LASEE509 pKa = 5.4 LKK511 pKa = 10.48 RR512 pKa = 11.84 CDD514 pKa = 3.86 FNCFHH519 pKa = 7.03 VNNEE523 pKa = 4.27 CQCEE527 pKa = 3.99 DD528 pKa = 3.35 RR529 pKa = 11.84 RR530 pKa = 11.84 VHH532 pKa = 6.57 RR533 pKa = 11.84 FKK535 pKa = 11.06 RR536 pKa = 11.84 DD537 pKa = 3.19 LSSNNLGHH545 pKa = 6.11 YY546 pKa = 9.45 PIVSPKK552 pKa = 10.81 GFINYY557 pKa = 9.49 NSTYY561 pKa = 10.55 QPTSDD566 pKa = 5.24 DD567 pKa = 3.42 EE568 pKa = 4.76 TIQLTFNTITKK579 pKa = 8.93 TPTTSVVSGEE589 pKa = 4.34 SIYY592 pKa = 11.08 RR593 pKa = 11.84 MPFQNNEE600 pKa = 4.22 GISFRR605 pKa = 11.84 ISTDD609 pKa = 2.49 EE610 pKa = 4.07 DD611 pKa = 4.32 FEE613 pKa = 4.86 VKK615 pKa = 9.82 TISVSLHH622 pKa = 6.84 DD623 pKa = 4.4 AFQVYY628 pKa = 9.59 KK629 pKa = 10.64 SKK631 pKa = 10.43 FVKK634 pKa = 9.27 WVSDD638 pKa = 3.31 RR639 pKa = 11.84 TLKK642 pKa = 10.47 ISEE645 pKa = 4.31 EE646 pKa = 4.24 FGCTGSCDD654 pKa = 4.05 DD655 pKa = 4.44 LCKK658 pKa = 10.84 CEE660 pKa = 4.87 ISTCKK665 pKa = 8.46 VAKK668 pKa = 9.14 WQDD671 pKa = 3.28 DD672 pKa = 3.99 RR673 pKa = 11.84 GWGCNPSWCWSISDD687 pKa = 4.67 GCTCCKK693 pKa = 10.56 ASALEE698 pKa = 4.1 NFSSGLLGIWEE709 pKa = 4.33 VEE711 pKa = 3.6 LSYY714 pKa = 11.13 FGLTLCLSDD723 pKa = 5.0 SMLSYY728 pKa = 9.87 KK729 pKa = 10.27 CKK731 pKa = 10.01 YY732 pKa = 10.51 LEE734 pKa = 4.56 GPGTFLIGKK743 pKa = 9.07 YY744 pKa = 8.6 EE745 pKa = 3.9 LSIGNPTGNIKK756 pKa = 10.29 KK757 pKa = 9.49 VSKK760 pKa = 10.64 FIATKK765 pKa = 9.99 HH766 pKa = 5.55 NVGQTHH772 pKa = 6.26 SNIDD776 pKa = 3.79 QIVEE780 pKa = 4.14 IYY782 pKa = 10.4 SDD784 pKa = 3.97 PNICYY789 pKa = 9.71 QEE791 pKa = 4.35 HH792 pKa = 6.2 CTHH795 pKa = 6.81 GEE797 pKa = 3.48 IGDD800 pKa = 3.59 YY801 pKa = 9.86 SFRR804 pKa = 11.84 SIEE807 pKa = 4.85 DD808 pKa = 2.83 ISTYY812 pKa = 10.98 ADD814 pKa = 3.66 KK815 pKa = 11.22 KK816 pKa = 7.86 PTTYY820 pKa = 9.91 MVWKK824 pKa = 6.25 TTSLSRR830 pKa = 11.84 QCSFGNYY837 pKa = 6.91 PKK839 pKa = 10.43 CYY841 pKa = 9.35 SDD843 pKa = 5.78 DD844 pKa = 3.94 MVSSLSDD851 pKa = 3.1 KK852 pKa = 10.74 FDD854 pKa = 3.4 YY855 pKa = 10.16 FYY857 pKa = 11.43 KK858 pKa = 10.67 NKK860 pKa = 10.24 QILKK864 pKa = 8.45 NDD866 pKa = 3.3 FRR868 pKa = 11.84 IVTSTLNQDD877 pKa = 3.11 RR878 pKa = 11.84 SGNPSLVLKK887 pKa = 8.23 VQPKK891 pKa = 8.81 MNYY894 pKa = 9.84 GYY896 pKa = 10.06 IEE898 pKa = 3.93 GLIKK902 pKa = 10.74 VRR904 pKa = 11.84 GLEE907 pKa = 4.05 LQHH910 pKa = 6.82 KK911 pKa = 8.47 SVSVKK916 pKa = 9.97 VDD918 pKa = 2.87 TYY920 pKa = 11.17 ILKK923 pKa = 8.91 TCGGCRR929 pKa = 11.84 NCRR932 pKa = 11.84 RR933 pKa = 11.84 GFWCTLRR940 pKa = 11.84 LSISEE945 pKa = 3.91 PLKK948 pKa = 11.04 FNIHH952 pKa = 6.91 LYY954 pKa = 10.76 SEE956 pKa = 5.0 DD957 pKa = 3.45 PTVAIDD963 pKa = 3.27 TRR965 pKa = 11.84 TIEE968 pKa = 3.89 VGRR971 pKa = 11.84 YY972 pKa = 7.28 PKK974 pKa = 10.33 DD975 pKa = 3.48 YY976 pKa = 9.31 NVSMFTPILIRR987 pKa = 11.84 EE988 pKa = 4.02 VKK990 pKa = 9.98 ICIKK994 pKa = 10.31 EE995 pKa = 3.73 VDD997 pKa = 3.32 MCKK1000 pKa = 9.9 TIKK1003 pKa = 10.57 NISLDD1008 pKa = 3.66 EE1009 pKa = 4.13 PSFYY1013 pKa = 10.12 LTHH1016 pKa = 6.79 QGDD1019 pKa = 4.41 SIYY1022 pKa = 11.16 SSLPNKK1028 pKa = 9.94 TYY1030 pKa = 10.76 DD1031 pKa = 3.7 GQNLFYY1037 pKa = 10.65 SIYY1040 pKa = 10.61 GGIKK1044 pKa = 8.2 ATTVAMFDD1052 pKa = 3.72 VIRR1055 pKa = 11.84 GFWSNIYY1062 pKa = 9.42 IVLMVASVLVLLIFLKK1078 pKa = 11.01 YY1079 pKa = 10.32 LGLLRR1084 pKa = 11.84 LVFDD1088 pKa = 3.82 VLTCGFCRR1096 pKa = 11.84 RR1097 pKa = 11.84 KK1098 pKa = 10.13 VRR1100 pKa = 11.84 YY1101 pKa = 9.22 NKK1103 pKa = 10.23 SAEE1106 pKa = 4.11 KK1107 pKa = 10.52 KK1108 pKa = 9.6 PLLKK1112 pKa = 9.88 RR1113 pKa = 11.84 TT1114 pKa = 3.72
Molecular weight: 127.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.145
IPC2_protein 7.307
IPC_protein 7.059
Toseland 6.678
ProMoST 7.702
Dawson 7.775
Bjellqvist 8.302
Wikipedia 7.673
Rodwell 7.805
Grimsley 6.722
Solomon 7.79
Lehninger 7.819
Nozaki 8.565
DTASelect 7.951
Thurlkill 7.995
EMBOSS 7.995
Sillero 8.375
Patrickios 3.401
IPC_peptide 7.79
IPC2_peptide 7.424
IPC2.peptide.svr19 7.321
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
5533
485
3934
1844.3
210.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.832 ± 1.288
2.223 ± 0.793
5.476 ± 0.058
6.524 ± 0.985
4.573 ± 0.179
4.301 ± 0.282
1.952 ± 0.122
8.531 ± 0.622
7.808 ± 0.895
10.248 ± 0.534
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.458 ± 0.416
5.675 ± 0.25
2.91 ± 0.3
3.56 ± 0.192
4.103 ± 0.316
10.645 ± 1.048
5.765 ± 0.729
4.753 ± 0.743
0.976 ± 0.121
3.687 ± 0.462
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here