Kimberley virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ephemerovirus; Kimberley ephemerovirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9UGX0|J9UGX0_9RHAB Isoform of J9TGT0 Alpha2 protein OS=Kimberley virus OX=318835 GN=alpha PE=4 SV=1
MM1 pKa = 7.26EE2 pKa = 5.37HH3 pKa = 6.9FNPKK7 pKa = 8.9EE8 pKa = 3.85VCRR11 pKa = 11.84SYY13 pKa = 11.85DD14 pKa = 3.75LAALMNNIKK23 pKa = 10.27DD24 pKa = 3.51IPEE27 pKa = 4.48DD28 pKa = 3.98GEE30 pKa = 4.39LDD32 pKa = 3.63EE33 pKa = 4.85NQEE36 pKa = 4.1EE37 pKa = 4.83KK38 pKa = 9.68PTGKK42 pKa = 10.14NSLVGRR48 pKa = 11.84DD49 pKa = 3.62LSSKK53 pKa = 10.01KK54 pKa = 10.35VYY56 pKa = 9.67PVEE59 pKa = 3.81IRR61 pKa = 11.84LEE63 pKa = 3.94EE64 pKa = 4.27LEE66 pKa = 4.51EE67 pKa = 4.56KK68 pKa = 11.23GNDD71 pKa = 3.63DD72 pKa = 5.5LDD74 pKa = 3.83WEE76 pKa = 4.8GDD78 pKa = 3.53LLKK81 pKa = 10.32IVEE84 pKa = 4.45SCEE87 pKa = 3.77SDD89 pKa = 3.09KK90 pKa = 11.63SVDD93 pKa = 3.04IYY95 pKa = 10.71EE96 pKa = 4.51EE97 pKa = 4.53KK98 pKa = 10.62DD99 pKa = 3.22LKK101 pKa = 11.21HH102 pKa = 6.4PIKK105 pKa = 10.37PSEE108 pKa = 4.29EE109 pKa = 4.08YY110 pKa = 10.51FPRR113 pKa = 11.84DD114 pKa = 3.01PVRR117 pKa = 11.84SVKK120 pKa = 10.3KK121 pKa = 10.25PKK123 pKa = 10.28DD124 pKa = 3.59GLLMDD129 pKa = 4.44IACPILIGVGEE140 pKa = 4.05INKK143 pKa = 9.47IVGILEE149 pKa = 4.12FFNLYY154 pKa = 10.45HH155 pKa = 7.45DD156 pKa = 3.74IDD158 pKa = 4.97YY159 pKa = 11.11NLEE162 pKa = 3.53WDD164 pKa = 3.8EE165 pKa = 5.54RR166 pKa = 11.84GMIMIKK172 pKa = 10.17KK173 pKa = 10.01AGVSNCGPSTINKK186 pKa = 8.14TSSTGQSDD194 pKa = 3.49NKK196 pKa = 10.46LFNGVMDD203 pKa = 5.0TINRR207 pKa = 11.84GIRR210 pKa = 11.84IKK212 pKa = 10.62KK213 pKa = 9.84KK214 pKa = 8.99YY215 pKa = 10.37GKK217 pKa = 10.62GFLKK221 pKa = 9.93IDD223 pKa = 3.94AEE225 pKa = 4.21NLPITHH231 pKa = 6.71HH232 pKa = 6.74EE233 pKa = 3.96IYY235 pKa = 10.95GIIQKK240 pKa = 9.08MDD242 pKa = 3.43QNLKK246 pKa = 7.67TTDD249 pKa = 3.46GLKK252 pKa = 10.84LLLRR256 pKa = 11.84KK257 pKa = 9.91AKK259 pKa = 8.67GTKK262 pKa = 10.53SMVKK266 pKa = 10.5SMDD269 pKa = 3.46LDD271 pKa = 4.1NIIFLL276 pKa = 4.51

Molecular weight: 31.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9U0Z2|J9U0Z2_9RHAB Non-structural transmembrane glycoprotein OS=Kimberley virus OX=318835 GN=GNS PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.77KK3 pKa = 10.69FPFQKK8 pKa = 10.55PFDD11 pKa = 4.72EE12 pKa = 4.4IKK14 pKa = 10.6NWLTEE19 pKa = 4.09SRR21 pKa = 11.84DD22 pKa = 4.3KK23 pKa = 11.06ISNWWNLTEE32 pKa = 3.73WRR34 pKa = 11.84IRR36 pKa = 11.84LGFYY40 pKa = 10.29IIISLIIGIVLSRR53 pKa = 11.84ILIKK57 pKa = 10.26IFKK60 pKa = 9.54CINTGVSGVRR70 pKa = 11.84KK71 pKa = 9.21LKK73 pKa = 10.67KK74 pKa = 9.55IIKK77 pKa = 9.45RR78 pKa = 11.84KK79 pKa = 10.07NKK81 pKa = 9.71VKK83 pKa = 10.38SQHH86 pKa = 5.11SLKK89 pKa = 10.62GKK91 pKa = 10.04KK92 pKa = 9.42KK93 pKa = 10.52LSIAKK98 pKa = 9.63FKK100 pKa = 11.21KK101 pKa = 10.62FGDD104 pKa = 3.69KK105 pKa = 10.77

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

1

9

4627

92

2139

514.1

59.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.723 ± 0.282

2.032 ± 0.207

6.138 ± 0.462

7.759 ± 0.372

3.804 ± 0.176

6.181 ± 0.216

2.291 ± 0.151

8.299 ± 0.56

9.099 ± 0.833

9.228 ± 0.851

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.283

6.57 ± 0.23

3.393 ± 0.266

2.572 ± 0.343

4.733 ± 0.309

7.154 ± 0.383

4.106 ± 0.278

5.144 ± 0.304

1.837 ± 0.221

4.214 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski