La Joya virus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1C8|A0A0D3R1C8_9RHAB Uncharacterized protein OS=La Joya virus OX=1272946 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 4.86 FNLDD6 pKa = 3.64 QEE8 pKa = 4.88 EE9 pKa = 4.4 KK10 pKa = 10.36 SHH12 pKa = 6.71 PLFFNVEE19 pKa = 3.31 IHH21 pKa = 5.65 IGYY24 pKa = 9.42 RR25 pKa = 11.84 GPYY28 pKa = 9.17 FDD30 pKa = 6.46 SNDD33 pKa = 3.96 LLTQIDD39 pKa = 3.98 KK40 pKa = 10.25 MIEE43 pKa = 4.03 SRR45 pKa = 11.84 PSSSNHH51 pKa = 5.27 TDD53 pKa = 4.49 LIRR56 pKa = 11.84 LAIGLGLAHH65 pKa = 6.75 CVHH68 pKa = 6.2 SQITPTLWNLNGSFMGVLKK87 pKa = 11.04 LPDD90 pKa = 3.73 YY91 pKa = 10.12 PVNPTVHH98 pKa = 5.39 TVVGQEE104 pKa = 3.74 ITDD107 pKa = 3.9 GCGLLYY113 pKa = 10.11 PDD115 pKa = 3.84 ISVYY119 pKa = 10.41 SKK121 pKa = 10.6 IYY123 pKa = 10.34 IMLGNEE129 pKa = 4.48 SKK131 pKa = 10.76 VQDD134 pKa = 4.95 IITHH138 pKa = 6.74 WYY140 pKa = 7.14 TGEE143 pKa = 4.17 GGVCYY148 pKa = 9.65 EE149 pKa = 4.65 YY150 pKa = 11.1 DD151 pKa = 3.39 FSLPTVAGALKK162 pKa = 10.41 FEE164 pKa = 4.5 HH165 pKa = 6.92 LFLL168 pKa = 6.29
Molecular weight: 18.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.765
IPC2_protein 4.851
IPC_protein 4.774
Toseland 4.724
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.914
Wikipedia 4.698
Rodwell 4.698
Grimsley 4.647
Solomon 4.787
Lehninger 4.749
Nozaki 4.927
DTASelect 5.105
Thurlkill 4.736
EMBOSS 4.736
Sillero 4.978
Patrickios 0.947
IPC_peptide 4.8
IPC2_peptide 4.978
IPC2.peptide.svr19 4.837
Protein with the highest isoelectric point:
>tr|A0A0D3R1B8|A0A0D3R1B8_9RHAB Glycoprotein OS=La Joya virus OX=1272946 PE=4 SV=1
MM1 pKa = 7.41 IKK3 pKa = 10.14 EE4 pKa = 3.85 FSKK7 pKa = 10.43 IGKK10 pKa = 8.68 NLLDD14 pKa = 3.88 MKK16 pKa = 9.4 WYY18 pKa = 9.76 CPSIARR24 pKa = 11.84 GSGSKK29 pKa = 10.4 SLLCHH34 pKa = 7.08 LLAPNGRR41 pKa = 11.84 LMDD44 pKa = 4.53 PMDD47 pKa = 3.58 IMMNISPKK55 pKa = 10.5 CGIHH59 pKa = 7.58 LLMPVLRR66 pKa = 11.84 SKK68 pKa = 10.16 GICVKK73 pKa = 10.23 KK74 pKa = 10.45 RR75 pKa = 11.84 PGMPNVKK82 pKa = 10.09 KK83 pKa = 10.81 LGTFQPLKK91 pKa = 9.89 AQPLMRR97 pKa = 11.84 HH98 pKa = 6.16 PLTRR102 pKa = 11.84 MTVEE106 pKa = 3.82 LHH108 pKa = 5.28 SFYY111 pKa = 10.58 IKK113 pKa = 10.32 RR114 pKa = 11.84 VNSWSPSSHH123 pKa = 6.39 HH124 pKa = 5.96 SHH126 pKa = 7.45 AIGITSTSTLKK137 pKa = 10.84 HH138 pKa = 5.61 LL139 pKa = 4.54
Molecular weight: 15.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.229
IPC2_protein 9.604
IPC_protein 9.633
Toseland 10.687
ProMoST 10.145
Dawson 10.76
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 11.389
Grimsley 10.774
Solomon 10.789
Lehninger 10.789
Nozaki 10.672
DTASelect 10.335
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.672
Patrickios 11.125
IPC_peptide 10.804
IPC2_peptide 9.151
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
5028
69
2119
359.1
40.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.898 ± 0.502
1.71 ± 0.202
5.469 ± 0.314
5.111 ± 0.29
5.052 ± 0.295
6.046 ± 0.261
3.142 ± 0.267
7.359 ± 0.36
6.543 ± 0.374
10.561 ± 0.524
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.566 ± 0.225
4.873 ± 0.223
5.251 ± 0.337
3.142 ± 0.202
4.018 ± 0.335
8.473 ± 0.425
5.529 ± 0.251
5.27 ± 0.377
1.909 ± 0.165
4.077 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here