Porcine circovirus-like virus P1
Average proteome isoelectric point is 7.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U3ZBW1|A0A0U3ZBW1_9CIRC ORF1 OS=Porcine circovirus-like virus P1 OX=1506546 PE=3 SV=1
MM1 pKa = 7.12 MRR3 pKa = 11.84 FNIDD7 pKa = 3.37 DD8 pKa = 4.26 FVPPGGGTNKK18 pKa = 9.72 ISIPFEE24 pKa = 3.79 YY25 pKa = 10.76 YY26 pKa = 10.19 RR27 pKa = 11.84 IRR29 pKa = 11.84 KK30 pKa = 8.77 VKK32 pKa = 10.6 VEE34 pKa = 4.87 FWPCSPITQGDD45 pKa = 3.7 RR46 pKa = 11.84 GVGTTAVILDD56 pKa = 3.99 DD57 pKa = 4.11 NFVPKK62 pKa = 9.8 ATAQTYY68 pKa = 10.24 DD69 pKa = 3.96 PYY71 pKa = 10.92 VTTPPAIQSPNPSPTTPVTSHH92 pKa = 7.23 PNLFLTPLLITSDD105 pKa = 4.05 QITKK109 pKa = 10.57 GISFGG114 pKa = 3.6
Molecular weight: 12.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.234
IPC2_protein 6.135
IPC_protein 6.605
Toseland 6.605
ProMoST 6.766
Dawson 6.971
Bjellqvist 6.532
Wikipedia 6.971
Rodwell 6.912
Grimsley 6.634
Solomon 7.146
Lehninger 7.146
Nozaki 6.898
DTASelect 7.176
Thurlkill 7.19
EMBOSS 7.351
Sillero 7.249
Patrickios 4.202
IPC_peptide 7.146
IPC2_peptide 6.825
IPC2.peptide.svr19 6.936
Protein with the highest isoelectric point:
>tr|A0A0U3ZBW1|A0A0U3ZBW1_9CIRC ORF1 OS=Porcine circovirus-like virus P1 OX=1506546 PE=3 SV=1
MM1 pKa = 7.03 STAQEE6 pKa = 4.22 GVLTVVALIVYY17 pKa = 7.11 PKK19 pKa = 9.25 VRR21 pKa = 11.84 EE22 pKa = 3.91 RR23 pKa = 11.84 RR24 pKa = 11.84 VLKK27 pKa = 10.27 MPFFLLQRR35 pKa = 4.0
Molecular weight: 4.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.911
IPC_protein 10.965
Toseland 11.067
ProMoST 11.623
Dawson 11.125
Bjellqvist 10.935
Wikipedia 11.433
Rodwell 11.213
Grimsley 11.169
Solomon 11.389
Lehninger 11.345
Nozaki 11.052
DTASelect 10.935
Thurlkill 11.067
EMBOSS 11.506
Sillero 11.082
Patrickios 11.082
IPC_peptide 11.403
IPC2_peptide 10.058
IPC2.peptide.svr19 8.881
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
149
35
114
74.5
8.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.027 ± 0.87
0.671 ± 0.346
4.698 ± 2.422
2.685 ± 1.562
6.04 ± 0.168
6.04 ± 1.641
0.671 ± 0.346
7.383 ± 2.333
4.698 ± 0.524
6.711 ± 3.905
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.685 ± 1.562
3.356 ± 1.73
11.409 ± 2.936
4.027 ± 0.87
5.369 ± 3.124
5.369 ± 1.295
10.738 ± 2.59
9.396 ± 3.994
0.671 ± 0.346
3.356 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here