Amino acid dipepetide frequency for bacterium HR26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.261AlaAla: 15.261 ± 0.211	1.077AlaCys: 1.077 ± 0.044	5.269AlaAsp: 5.269 ± 0.075	8.701AlaGlu: 8.701 ± 0.133	3.584AlaPhe: 3.584 ± 0.074	10.986AlaGly: 10.986 ± 0.153	2.129AlaHis: 2.129 ± 0.058	6.045AlaIle: 6.045 ± 0.091	1.811AlaLys: 1.811 ± 0.055	14.823AlaLeu: 14.823 ± 0.186	2.566AlaMet: 2.566 ± 0.06	1.88AlaAsn: 1.88 ± 0.058	5.22AlaPro: 5.22 ± 0.089	4.105AlaGln: 4.105 ± 0.095	10.526AlaArg: 10.526 ± 0.139	5.413AlaSer: 5.413 ± 0.089	5.534AlaThr: 5.534 ± 0.105	9.808AlaVal: 9.808 ± 0.137	1.818AlaTrp: 1.818 ± 0.054	2.576AlaTyr: 2.576 ± 0.064	0.0AlaXaa: 0.0 ± 0.0
Cys	0.763CysAla: 0.763 ± 0.034	0.121CysCys: 0.121 ± 0.014	0.399CysAsp: 0.399 ± 0.023	0.403CysGlu: 0.403 ± 0.023	0.263CysPhe: 0.263 ± 0.021	0.97CysGly: 0.97 ± 0.046	0.232CysHis: 0.232 ± 0.02	0.29CysIle: 0.29 ± 0.02	0.092CysLys: 0.092 ± 0.011	0.741CysLeu: 0.741 ± 0.033	0.115CysMet: 0.115 ± 0.013	0.14CysAsn: 0.14 ± 0.014	0.658CysPro: 0.658 ± 0.033	0.271CysGln: 0.271 ± 0.019	0.788CysArg: 0.788 ± 0.036	0.373CysSer: 0.373 ± 0.023	0.381CysThr: 0.381 ± 0.024	0.475CysVal: 0.475 ± 0.025	0.129CysTrp: 0.129 ± 0.014	0.236CysTyr: 0.236 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.973AspAla: 4.973 ± 0.085	0.338AspCys: 0.338 ± 0.027	2.522AspAsp: 2.522 ± 0.061	3.702AspGlu: 3.702 ± 0.086	1.534AspPhe: 1.534 ± 0.046	4.29AspGly: 4.29 ± 0.083	1.08AspHis: 1.08 ± 0.043	2.059AspIle: 2.059 ± 0.065	0.662AspLys: 0.662 ± 0.034	6.061AspLeu: 6.061 ± 0.086	0.671AspMet: 0.671 ± 0.031	0.704AspAsn: 0.704 ± 0.037	4.255AspPro: 4.255 ± 0.075	1.791AspGln: 1.791 ± 0.049	4.597AspArg: 4.597 ± 0.075	1.588AspSer: 1.588 ± 0.051	1.932AspThr: 1.932 ± 0.051	3.92AspVal: 3.92 ± 0.082	0.81AspTrp: 0.81 ± 0.035	1.359AspTyr: 1.359 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	8.419GluAla: 8.419 ± 0.118	0.318GluCys: 0.318 ± 0.023	2.617GluAsp: 2.617 ± 0.063	4.909GluGlu: 4.909 ± 0.095	1.789GluPhe: 1.789 ± 0.055	4.438GluGly: 4.438 ± 0.085	1.728GluHis: 1.728 ± 0.054	3.57GluIle: 3.57 ± 0.076	1.029GluLys: 1.029 ± 0.045	8.585GluLeu: 8.585 ± 0.129	1.244GluMet: 1.244 ± 0.039	0.992GluAsn: 0.992 ± 0.037	4.17GluPro: 4.17 ± 0.08	3.311GluGln: 3.311 ± 0.079	8.19GluArg: 8.19 ± 0.127	2.503GluSer: 2.503 ± 0.061	3.135GluThr: 3.135 ± 0.068	5.395GluVal: 5.395 ± 0.096	0.804GluTrp: 0.804 ± 0.031	1.329GluTyr: 1.329 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.351PheAla: 3.351 ± 0.08	0.24PheCys: 0.24 ± 0.018	1.917PheAsp: 1.917 ± 0.056	1.851PheGlu: 1.851 ± 0.059	1.023PhePhe: 1.023 ± 0.04	3.118PheGly: 3.118 ± 0.071	0.638PheHis: 0.638 ± 0.029	1.378PheIle: 1.378 ± 0.043	0.512PheLys: 0.512 ± 0.028	3.399PheLeu: 3.399 ± 0.073	0.474PheMet: 0.474 ± 0.026	0.688PheAsn: 0.688 ± 0.034	1.711PhePro: 1.711 ± 0.045	0.995PheGln: 0.995 ± 0.041	2.296PheArg: 2.296 ± 0.06	1.532PheSer: 1.532 ± 0.051	1.789PheThr: 1.789 ± 0.048	2.451PheVal: 2.451 ± 0.057	0.566PheTrp: 0.566 ± 0.028	0.801PheTyr: 0.801 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	8.386GlyAla: 8.386 ± 0.141	0.866GlyCys: 0.866 ± 0.039	4.079GlyAsp: 4.079 ± 0.092	5.468GlyGlu: 5.468 ± 0.09	2.954GlyPhe: 2.954 ± 0.065	6.671GlyGly: 6.671 ± 0.128	1.985GlyHis: 1.985 ± 0.062	4.592GlyIle: 4.592 ± 0.089	1.908GlyLys: 1.908 ± 0.058	9.399GlyLeu: 9.399 ± 0.134	2.056GlyMet: 2.056 ± 0.051	1.625GlyAsn: 1.625 ± 0.053	4.166GlyPro: 4.166 ± 0.068	3.572GlyGln: 3.572 ± 0.076	7.27GlyArg: 7.27 ± 0.119	4.457GlySer: 4.457 ± 0.079	4.375GlyThr: 4.375 ± 0.076	6.565GlyVal: 6.565 ± 0.105	1.556GlyTrp: 1.556 ± 0.046	2.515GlyTyr: 2.515 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	2.152HisAla: 2.152 ± 0.054	0.223HisCys: 0.223 ± 0.016	1.052HisAsp: 1.052 ± 0.034	1.263HisGlu: 1.263 ± 0.039	0.654HisPhe: 0.654 ± 0.028	1.922HisGly: 1.922 ± 0.051	0.585HisHis: 0.585 ± 0.03	0.832HisIle: 0.832 ± 0.036	0.279HisLys: 0.279 ± 0.019	2.418HisLeu: 2.418 ± 0.058	0.248HisMet: 0.248 ± 0.018	0.389HisAsn: 0.389 ± 0.023	1.739HisPro: 1.739 ± 0.056	0.721HisGln: 0.721 ± 0.03	1.985HisArg: 1.985 ± 0.056	0.817HisSer: 0.817 ± 0.035	0.84HisThr: 0.84 ± 0.034	1.552HisVal: 1.552 ± 0.053	0.327HisTrp: 0.327 ± 0.021	0.619HisTyr: 0.619 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.253IleAla: 6.253 ± 0.102	0.386IleCys: 0.386 ± 0.022	3.033IleAsp: 3.033 ± 0.062	3.82IleGlu: 3.82 ± 0.078	1.389IlePhe: 1.389 ± 0.054	4.476IleGly: 4.476 ± 0.085	0.907IleHis: 0.907 ± 0.038	1.995IleIle: 1.995 ± 0.062	0.825IleLys: 0.825 ± 0.042	4.534IleLeu: 4.534 ± 0.091	0.655IleMet: 0.655 ± 0.029	0.974IleAsn: 0.974 ± 0.036	2.943IlePro: 2.943 ± 0.067	1.443IleGln: 1.443 ± 0.048	3.488IleArg: 3.488 ± 0.075	2.096IleSer: 2.096 ± 0.06	2.503IleThr: 2.503 ± 0.066	4.416IleVal: 4.416 ± 0.085	0.626IleTrp: 0.626 ± 0.031	1.134IleTyr: 1.134 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	1.806LysAla: 1.806 ± 0.06	0.084LysCys: 0.084 ± 0.013	0.685LysAsp: 0.685 ± 0.034	0.999LysGlu: 0.999 ± 0.042	0.4LysPhe: 0.4 ± 0.025	1.174LysGly: 1.174 ± 0.048	0.325LysHis: 0.325 ± 0.022	0.855LysIle: 0.855 ± 0.046	0.396LysLys: 0.396 ± 0.028	1.914LysLeu: 1.914 ± 0.054	0.264LysMet: 0.264 ± 0.023	0.327LysAsn: 0.327 ± 0.022	1.145LysPro: 1.145 ± 0.042	0.607LysGln: 0.607 ± 0.031	1.517LysArg: 1.517 ± 0.049	0.693LysSer: 0.693 ± 0.033	0.982LysThr: 0.982 ± 0.039	1.403LysVal: 1.403 ± 0.052	0.193LysTrp: 0.193 ± 0.016	0.396LysTyr: 0.396 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	16.98LeuAla: 16.98 ± 0.227	0.841LeuCys: 0.841 ± 0.036	6.224LeuAsp: 6.224 ± 0.108	7.411LeuGlu: 7.411 ± 0.103	3.325LeuPhe: 3.325 ± 0.074	10.044LeuGly: 10.044 ± 0.128	2.129LeuHis: 2.129 ± 0.057	5.249LeuIle: 5.249 ± 0.089	1.813LeuLys: 1.813 ± 0.063	13.468LeuLeu: 13.468 ± 0.22	1.769LeuMet: 1.769 ± 0.052	1.932LeuAsn: 1.932 ± 0.051	6.869LeuPro: 6.869 ± 0.116	3.384LeuGln: 3.384 ± 0.066	9.778LeuArg: 9.778 ± 0.13	5.664LeuSer: 5.664 ± 0.105	5.549LeuThr: 5.549 ± 0.095	10.018LeuVal: 10.018 ± 0.127	1.497LeuTrp: 1.497 ± 0.05	2.45LeuTyr: 2.45 ± 0.075	0.0LeuXaa: 0.0 ± 0.0
Met	2.466MetAla: 2.466 ± 0.059	0.092MetCys: 0.092 ± 0.012	0.733MetAsp: 0.733 ± 0.036	0.921MetGlu: 0.921 ± 0.032	0.396MetPhe: 0.396 ± 0.025	1.337MetGly: 1.337 ± 0.042	0.304MetHis: 0.304 ± 0.02	0.977MetIle: 0.977 ± 0.035	0.367MetLys: 0.367 ± 0.021	2.045MetLeu: 2.045 ± 0.055	0.393MetMet: 0.393 ± 0.023	0.377MetAsn: 0.377 ± 0.025	1.252MetPro: 1.252 ± 0.043	0.57MetGln: 0.57 ± 0.028	1.5MetArg: 1.5 ± 0.047	1.147MetSer: 1.147 ± 0.038	1.243MetThr: 1.243 ± 0.05	1.565MetVal: 1.565 ± 0.05	0.17MetTrp: 0.17 ± 0.015	0.325MetTyr: 0.325 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	1.972AsnAla: 1.972 ± 0.054	0.179AsnCys: 0.179 ± 0.018	0.759AsnAsp: 0.759 ± 0.035	0.932AsnGlu: 0.932 ± 0.035	0.586AsnPhe: 0.586 ± 0.03	1.513AsnGly: 1.513 ± 0.056	0.321AsnHis: 0.321 ± 0.023	0.829AsnIle: 0.829 ± 0.038	0.269AsnLys: 0.269 ± 0.021	2.161AsnLeu: 2.161 ± 0.058	0.286AsnMet: 0.286 ± 0.02	0.337AsnAsn: 0.337 ± 0.021	1.645AsnPro: 1.645 ± 0.058	0.607AsnGln: 0.607 ± 0.033	1.544AsnArg: 1.544 ± 0.051	0.693AsnSer: 0.693 ± 0.035	0.886AsnThr: 0.886 ± 0.035	1.545AsnVal: 1.545 ± 0.052	0.311AsnTrp: 0.311 ± 0.023	0.485AsnTyr: 0.485 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	7.541ProAla: 7.541 ± 0.111	0.345ProCys: 0.345 ± 0.024	3.877ProAsp: 3.877 ± 0.083	5.628ProGlu: 5.628 ± 0.094	1.841ProPhe: 1.841 ± 0.048	6.031ProGly: 6.031 ± 0.102	1.173ProHis: 1.173 ± 0.043	2.588ProIle: 2.588 ± 0.062	0.936ProLys: 0.936 ± 0.043	6.108ProLeu: 6.108 ± 0.101	0.997ProMet: 0.997 ± 0.034	1.267ProAsn: 1.267 ± 0.046	3.818ProPro: 3.818 ± 0.087	1.726ProGln: 1.726 ± 0.053	3.979ProArg: 3.979 ± 0.079	2.969ProSer: 2.969 ± 0.062	2.974ProThr: 2.974 ± 0.089	5.397ProVal: 5.397 ± 0.084	0.936ProTrp: 0.936 ± 0.037	1.511ProTyr: 1.511 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	5.121GlnAla: 5.121 ± 0.099	0.196GlnCys: 0.196 ± 0.017	1.215GlnAsp: 1.215 ± 0.04	2.262GlnGlu: 2.262 ± 0.058	0.98GlnPhe: 0.98 ± 0.036	2.44GlnGly: 2.44 ± 0.062	0.784GlnHis: 0.784 ± 0.034	1.75GlnIle: 1.75 ± 0.051	0.486GlnLys: 0.486 ± 0.026	3.881GlnLeu: 3.881 ± 0.084	0.703GlnMet: 0.703 ± 0.031	0.526GlnAsn: 0.526 ± 0.022	2.573GlnPro: 2.573 ± 0.065	1.584GlnGln: 1.584 ± 0.053	3.759GlnArg: 3.759 ± 0.077	1.289GlnSer: 1.289 ± 0.048	1.473GlnThr: 1.473 ± 0.047	3.336GlnVal: 3.336 ± 0.073	0.433GlnTrp: 0.433 ± 0.022	0.774GlnTyr: 0.774 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	9.733ArgAla: 9.733 ± 0.14	0.801ArgCys: 0.801 ± 0.038	3.931ArgAsp: 3.931 ± 0.097	6.404ArgGlu: 6.404 ± 0.1	2.963ArgPhe: 2.963 ± 0.064	6.106ArgGly: 6.106 ± 0.099	1.91ArgHis: 1.91 ± 0.056	4.081ArgIle: 4.081 ± 0.083	1.333ArgLys: 1.333 ± 0.052	11.106ArgLeu: 11.106 ± 0.153	1.74ArgMet: 1.74 ± 0.049	1.415ArgAsn: 1.415 ± 0.047	5.175ArgPro: 5.175 ± 0.092	3.796ArgGln: 3.796 ± 0.086	9.088ArgArg: 9.088 ± 0.142	4.106ArgSer: 4.106 ± 0.08	3.59ArgThr: 3.59 ± 0.065	6.687ArgVal: 6.687 ± 0.107	1.639ArgTrp: 1.639 ± 0.055	2.539ArgTyr: 2.539 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	4.709SerAla: 4.709 ± 0.08	0.34SerCys: 0.34 ± 0.025	2.096SerAsp: 2.096 ± 0.062	2.592SerGlu: 2.592 ± 0.058	1.61SerPhe: 1.61 ± 0.052	4.708SerGly: 4.708 ± 0.075	0.84SerHis: 0.84 ± 0.034	2.07SerIle: 2.07 ± 0.056	0.714SerLys: 0.714 ± 0.037	5.308SerLeu: 5.308 ± 0.1	1.022SerMet: 1.022 ± 0.039	0.834SerAsn: 0.834 ± 0.04	3.368SerPro: 3.368 ± 0.083	1.465SerGln: 1.465 ± 0.039	3.736SerArg: 3.736 ± 0.085	2.472SerSer: 2.472 ± 0.074	2.461SerThr: 2.461 ± 0.055	3.453SerVal: 3.453 ± 0.079	0.911SerTrp: 0.911 ± 0.038	1.104SerTyr: 1.104 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	5.467ThrAla: 5.467 ± 0.107	0.334ThrCys: 0.334 ± 0.022	2.036ThrAsp: 2.036 ± 0.059	2.522ThrGlu: 2.522 ± 0.061	1.677ThrPhe: 1.677 ± 0.059	4.845ThrGly: 4.845 ± 0.081	0.948ThrHis: 0.948 ± 0.037	2.677ThrIle: 2.677 ± 0.07	0.719ThrLys: 0.719 ± 0.035	5.879ThrLeu: 5.879 ± 0.096	0.928ThrMet: 0.928 ± 0.035	0.94ThrAsn: 0.94 ± 0.034	3.806ThrPro: 3.806 ± 0.098	1.248ThrGln: 1.248 ± 0.05	3.333ThrArg: 3.333 ± 0.059	2.306ThrSer: 2.306 ± 0.066	2.648ThrThr: 2.648 ± 0.072	4.625ThrVal: 4.625 ± 0.087	0.796ThrTrp: 0.796 ± 0.034	1.258ThrTyr: 1.258 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	9.816ValAla: 9.816 ± 0.14	0.636ValCys: 0.636 ± 0.03	4.333ValAsp: 4.333 ± 0.09	6.187ValGlu: 6.187 ± 0.1	2.369ValPhe: 2.369 ± 0.062	6.072ValGly: 6.072 ± 0.102	1.582ValHis: 1.582 ± 0.049	4.435ValIle: 4.435 ± 0.084	1.447ValLys: 1.447 ± 0.044	9.499ValLeu: 9.499 ± 0.119	1.44ValMet: 1.44 ± 0.049	1.678ValAsn: 1.678 ± 0.057	4.762ValPro: 4.762 ± 0.075	2.663ValGln: 2.663 ± 0.059	6.787ValArg: 6.787 ± 0.108	3.917ValSer: 3.917 ± 0.074	4.858ValThr: 4.858 ± 0.086	7.948ValVal: 7.948 ± 0.129	1.047ValTrp: 1.047 ± 0.04	1.926ValTyr: 1.926 ± 0.061	0.0ValXaa: 0.0 ± 0.0
Trp	1.428TrpAla: 1.428 ± 0.049	0.145TrpCys: 0.145 ± 0.016	0.736TrpAsp: 0.736 ± 0.032	0.954TrpGlu: 0.954 ± 0.033	0.517TrpPhe: 0.517 ± 0.03	1.069TrpGly: 1.069 ± 0.043	0.423TrpHis: 0.423 ± 0.023	0.754TrpIle: 0.754 ± 0.035	0.258TrpLys: 0.258 ± 0.017	2.041TrpLeu: 2.041 ± 0.058	0.329TrpMet: 0.329 ± 0.023	0.327TrpAsn: 0.327 ± 0.021	0.836TrpPro: 0.836 ± 0.038	0.695TrpGln: 0.695 ± 0.032	1.456TrpArg: 1.456 ± 0.048	0.821TrpSer: 0.821 ± 0.037	0.719TrpThr: 0.719 ± 0.034	1.11TrpVal: 1.11 ± 0.048	0.337TrpTrp: 0.337 ± 0.022	0.434TrpTyr: 0.434 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.535TyrAla: 2.535 ± 0.067	0.275TyrCys: 0.275 ± 0.023	1.334TyrAsp: 1.334 ± 0.042	1.548TyrGlu: 1.548 ± 0.051	0.877TyrPhe: 0.877 ± 0.042	2.256TyrGly: 2.256 ± 0.059	0.596TyrHis: 0.596 ± 0.027	0.87TyrIle: 0.87 ± 0.04	0.31TyrLys: 0.31 ± 0.02	3.025TyrLeu: 3.025 ± 0.073	0.277TyrMet: 0.277 ± 0.02	0.507TyrAsn: 0.507 ± 0.028	1.53TyrPro: 1.53 ± 0.046	1.012TyrGln: 1.012 ± 0.038	2.526TyrArg: 2.526 ± 0.061	0.956TyrSer: 0.956 ± 0.035	1.121TyrThr: 1.121 ± 0.044	1.734TyrVal: 1.734 ± 0.059	0.481TyrTrp: 0.481 ± 0.026	0.682TyrTyr: 0.682 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2404 proteins (729898 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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