Rhodobacter blasticus DSM 2131

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; Rhodobacter blasticus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3451 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4J8Z6|A0A2T4J8Z6_RHOBL TonB-dependent receptor OS=Rhodobacter blasticus DSM 2131 OX=1188250 GN=C5F44_10070 PE=3 SV=1
MM1 pKa = 7.26MATFTVVPVVTNPATQKK18 pKa = 10.17LQGVINVTAGDD29 pKa = 4.04TYY31 pKa = 10.82IIDD34 pKa = 3.72PTVSVGITFNPPAGTPLGTTVSYY57 pKa = 10.92NIQINAANASAGTAQRR73 pKa = 11.84LITLGDD79 pKa = 3.34QTTANITIAPGVNAGRR95 pKa = 11.84YY96 pKa = 8.71DD97 pKa = 3.73FGGGTVDD104 pKa = 3.59GANYY108 pKa = 9.31TIGAGATTGDD118 pKa = 3.97LQLGDD123 pKa = 4.39GGNGSLTTVTSSVTLGDD140 pKa = 3.66GATVAGQITGGAGADD155 pKa = 3.56TLTVGTNVTVTEE167 pKa = 5.17AINLGAGAASGTFGTGFSAQSFAASGAGAKK197 pKa = 9.37TFDD200 pKa = 4.2FSNTTTFTGTGASVSIASTNAPVSVDD226 pKa = 3.82FDD228 pKa = 5.52DD229 pKa = 4.75NLSTNGTISVSSSTGAATLDD249 pKa = 2.79IGANLKK255 pKa = 8.92TGNATLHH262 pKa = 5.77TGGAISVVGGGTGSANMLTIGLDD285 pKa = 3.4AQVNGAITINGSGGTNTLTIGDD307 pKa = 3.93SSEE310 pKa = 4.42VYY312 pKa = 10.73GFIYY316 pKa = 10.75ANGTNSAATVTIGDD330 pKa = 4.6LSTVTGDD337 pKa = 3.2IYY339 pKa = 11.47ANSGGTLALGNIDD352 pKa = 3.42NSVTVGDD359 pKa = 4.05GTTTGSIFVNGLYY372 pKa = 10.25SDD374 pKa = 3.76NTVSVGLASTVGNLYY389 pKa = 10.43FGTDD393 pKa = 3.5GTVSQTDD400 pKa = 3.62PTSGQVLTMADD411 pKa = 3.36NSTAGLIYY419 pKa = 9.91LTASNSTFDD428 pKa = 3.48VTFGTGVTTNAIYY441 pKa = 9.98GYY443 pKa = 10.48GYY445 pKa = 10.62NSDD448 pKa = 4.27YY449 pKa = 11.07NVSIPDD455 pKa = 3.83DD456 pKa = 3.85FTANGYY462 pKa = 10.58VYY464 pKa = 10.57FGSTNSTTDD473 pKa = 3.01VTIGNNAVINTGALDD488 pKa = 3.44ISGSDD493 pKa = 3.34VTTLIGDD500 pKa = 4.26GLSVQYY506 pKa = 9.85FASFGDD512 pKa = 3.91LNGATDD518 pKa = 3.5VTIGQGANIGGALYY532 pKa = 10.95ANGSTVDD539 pKa = 4.15FTAGPDD545 pKa = 3.68FDD547 pKa = 4.39VSGYY551 pKa = 11.44AYY553 pKa = 10.91LNDD556 pKa = 3.71ANATYY561 pKa = 9.65TIDD564 pKa = 3.74IGPGSDD570 pKa = 2.71IGGGFYY576 pKa = 11.1ANGFNSDD583 pKa = 3.45VTLTTGDD590 pKa = 3.59NFIVSLDD597 pKa = 3.65AYY599 pKa = 10.01IGGTDD604 pKa = 3.39GTTTVTLGSGSSIGTVLNVGGDD626 pKa = 3.89TVNFTAGTGLTTGGAVTFGDD646 pKa = 3.95AVSLSTTVNLTGPTVFGGLLDD667 pKa = 3.85VNGDD671 pKa = 3.61QIIFNGGPNLTVNGPAYY688 pKa = 7.9FTNDD692 pKa = 3.19GGTSTIVLGDD702 pKa = 3.31NTTINGLADD711 pKa = 4.32FAAPNQDD718 pKa = 2.66VSLTIGTNVTIGTIYY733 pKa = 11.01GDD735 pKa = 3.59TTGTGSFNMTTGDD748 pKa = 4.27DD749 pKa = 3.5FTFANFYY756 pKa = 11.13GGTTDD761 pKa = 3.53NDD763 pKa = 3.33EE764 pKa = 4.38HH765 pKa = 5.71VTIGKK770 pKa = 8.27NWTMTGSGVYY780 pKa = 10.0DD781 pKa = 3.79AGTGNDD787 pKa = 3.65TLILGTTADD796 pKa = 3.99RR797 pKa = 11.84SSTNYY802 pKa = 9.91IYY804 pKa = 10.9GGEE807 pKa = 4.2SANDD811 pKa = 3.27NDD813 pKa = 4.01VLTFRR818 pKa = 11.84LNAADD823 pKa = 3.49QASFTTAALAAGWTLNPDD841 pKa = 3.53GTFKK845 pKa = 8.57TTNGAGAHH853 pKa = 6.42IQLTWQGQVINYY865 pKa = 7.03WEE867 pKa = 4.21SVAFCFAQGTEE878 pKa = 3.75ISTDD882 pKa = 3.11AGPVKK887 pKa = 10.88VEE889 pKa = 4.02DD890 pKa = 4.17LRR892 pKa = 11.84PGSMVLTMDD901 pKa = 4.49RR902 pKa = 11.84GYY904 pKa = 11.0QPVAWIGGRR913 pKa = 11.84QLSGADD919 pKa = 3.64LQRR922 pKa = 11.84KK923 pKa = 8.18PNLRR927 pKa = 11.84PIRR930 pKa = 11.84IAAGALGHH938 pKa = 6.44GLPTADD944 pKa = 4.13LVVSPQHH951 pKa = 5.72RR952 pKa = 11.84VLVRR956 pKa = 11.84SRR958 pKa = 11.84LSEE961 pKa = 3.83RR962 pKa = 11.84MFSDD966 pKa = 3.69RR967 pKa = 11.84EE968 pKa = 3.78VLIAAKK974 pKa = 10.11HH975 pKa = 4.81LVGLPGITVAEE986 pKa = 4.22EE987 pKa = 3.97MDD989 pKa = 4.02GVSYY993 pKa = 9.36WHH995 pKa = 6.62FLFEE999 pKa = 3.89QHH1001 pKa = 6.78EE1002 pKa = 4.54IVYY1005 pKa = 10.9SNGAPTEE1012 pKa = 4.2SLFTGPEE1019 pKa = 3.72ALKK1022 pKa = 10.88ALTPDD1027 pKa = 3.31QQQEE1031 pKa = 4.07ILTLFPEE1038 pKa = 4.81LSEE1041 pKa = 4.05TDD1043 pKa = 3.17AVLNGDD1049 pKa = 3.84RR1050 pKa = 11.84LIPARR1055 pKa = 11.84TIVPGRR1061 pKa = 11.84LARR1064 pKa = 11.84SFTRR1068 pKa = 11.84RR1069 pKa = 11.84ATKK1072 pKa = 10.32RR1073 pKa = 11.84GASDD1077 pKa = 3.2VLSPVV1082 pKa = 3.02

Molecular weight: 109.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4J6X9|A0A2T4J6X9_RHOBL Transcriptional regulator OS=Rhodobacter blasticus DSM 2131 OX=1188250 GN=C5F44_13570 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.31QPSTLVRR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3451

0

3451

1074197

28

2804

311.3

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.784 ± 0.068

0.853 ± 0.012

5.692 ± 0.034

5.363 ± 0.038

3.538 ± 0.026

9.09 ± 0.043

1.981 ± 0.021

4.614 ± 0.028

2.843 ± 0.035

10.643 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.022

2.205 ± 0.025

5.674 ± 0.036

2.99 ± 0.019

7.206 ± 0.041

4.585 ± 0.027

5.376 ± 0.026

7.451 ± 0.037

1.473 ± 0.017

2.022 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski