Amino acid dipepetide frequency for Gordonia phage NosilaM

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.908AlaAla: 11.908 ± 1.152
0.703AlaCys: 0.703 ± 0.171
6.985AlaAsp: 6.985 ± 0.677
6.751AlaGlu: 6.751 ± 0.608
3.328AlaPhe: 3.328 ± 0.383
8.438AlaGly: 8.438 ± 0.746
2.391AlaHis: 2.391 ± 0.473
5.391AlaIle: 5.391 ± 0.524
5.251AlaLys: 5.251 ± 0.58
8.251AlaLeu: 8.251 ± 0.91
2.438AlaMet: 2.438 ± 0.307
3.891AlaAsn: 3.891 ± 0.435
4.688AlaPro: 4.688 ± 0.603
3.891AlaGln: 3.891 ± 0.403
5.672AlaArg: 5.672 ± 0.526
5.297AlaSer: 5.297 ± 0.619
6.376AlaThr: 6.376 ± 0.532
7.735AlaVal: 7.735 ± 0.515
1.735AlaTrp: 1.735 ± 0.257
2.11AlaTyr: 2.11 ± 0.274
0.0AlaXaa: 0.0 ± 0.0
Cys
0.656CysAla: 0.656 ± 0.174
0.047CysCys: 0.047 ± 0.044
0.75CysAsp: 0.75 ± 0.207
0.469CysGlu: 0.469 ± 0.155
0.047CysPhe: 0.047 ± 0.051
0.609CysGly: 0.609 ± 0.179
0.234CysHis: 0.234 ± 0.127
0.375CysIle: 0.375 ± 0.139
0.188CysLys: 0.188 ± 0.098
0.422CysLeu: 0.422 ± 0.134
0.0CysMet: 0.0 ± 0.0
0.141CysAsn: 0.141 ± 0.084
0.469CysPro: 0.469 ± 0.176
0.328CysGln: 0.328 ± 0.106
0.422CysArg: 0.422 ± 0.149
0.375CysSer: 0.375 ± 0.159
0.469CysThr: 0.469 ± 0.147
0.516CysVal: 0.516 ± 0.171
0.234CysTrp: 0.234 ± 0.105
0.281CysTyr: 0.281 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
7.829AspAla: 7.829 ± 0.541
0.375AspCys: 0.375 ± 0.156
5.86AspAsp: 5.86 ± 0.863
5.579AspGlu: 5.579 ± 0.713
2.625AspPhe: 2.625 ± 0.352
6.329AspGly: 6.329 ± 0.687
1.875AspHis: 1.875 ± 0.36
3.235AspIle: 3.235 ± 0.408
1.781AspLys: 1.781 ± 0.337
5.907AspLeu: 5.907 ± 0.492
1.5AspMet: 1.5 ± 0.232
1.781AspAsn: 1.781 ± 0.307
5.11AspPro: 5.11 ± 0.542
2.016AspGln: 2.016 ± 0.343
5.063AspArg: 5.063 ± 0.693
2.344AspSer: 2.344 ± 0.34
3.891AspThr: 3.891 ± 0.467
4.782AspVal: 4.782 ± 0.358
0.984AspTrp: 0.984 ± 0.218
2.016AspTyr: 2.016 ± 0.324
0.0AspXaa: 0.0 ± 0.0
Glu
6.61GluAla: 6.61 ± 0.529
0.422GluCys: 0.422 ± 0.153
4.969GluAsp: 4.969 ± 0.662
4.641GluGlu: 4.641 ± 0.729
2.11GluPhe: 2.11 ± 0.372
5.251GluGly: 5.251 ± 0.445
1.735GluHis: 1.735 ± 0.272
4.125GluIle: 4.125 ± 0.447
3.422GluLys: 3.422 ± 0.476
5.438GluLeu: 5.438 ± 0.598
1.735GluMet: 1.735 ± 0.303
1.969GluAsn: 1.969 ± 0.339
2.953GluPro: 2.953 ± 0.47
2.25GluGln: 2.25 ± 0.331
4.922GluArg: 4.922 ± 0.574
3.516GluSer: 3.516 ± 0.382
4.079GluThr: 4.079 ± 0.476
3.891GluVal: 3.891 ± 0.481
1.406GluTrp: 1.406 ± 0.238
2.25GluTyr: 2.25 ± 0.389
0.0GluXaa: 0.0 ± 0.0
Phe
3.0PheAla: 3.0 ± 0.351
0.234PheCys: 0.234 ± 0.106
2.438PheAsp: 2.438 ± 0.366
2.25PheGlu: 2.25 ± 0.273
0.703PhePhe: 0.703 ± 0.201
2.953PheGly: 2.953 ± 0.357
0.656PheHis: 0.656 ± 0.142
1.547PheIle: 1.547 ± 0.213
0.844PheLys: 0.844 ± 0.196
2.485PheLeu: 2.485 ± 0.375
0.703PheMet: 0.703 ± 0.143
1.125PheAsn: 1.125 ± 0.254
1.735PhePro: 1.735 ± 0.303
1.313PheGln: 1.313 ± 0.267
2.391PheArg: 2.391 ± 0.334
1.828PheSer: 1.828 ± 0.366
1.969PheThr: 1.969 ± 0.404
1.688PheVal: 1.688 ± 0.299
0.75PheTrp: 0.75 ± 0.168
1.172PheTyr: 1.172 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
8.345GlyAla: 8.345 ± 0.77
0.703GlyCys: 0.703 ± 0.203
6.376GlyAsp: 6.376 ± 0.64
6.938GlyGlu: 6.938 ± 0.701
2.953GlyPhe: 2.953 ± 0.313
7.782GlyGly: 7.782 ± 1.298
1.594GlyHis: 1.594 ± 0.321
3.797GlyIle: 3.797 ± 0.584
3.516GlyLys: 3.516 ± 0.405
6.563GlyLeu: 6.563 ± 0.769
2.016GlyMet: 2.016 ± 0.357
3.094GlyAsn: 3.094 ± 0.441
4.032GlyPro: 4.032 ± 0.502
3.0GlyGln: 3.0 ± 0.399
5.813GlyArg: 5.813 ± 0.553
5.11GlySer: 5.11 ± 0.6
5.766GlyThr: 5.766 ± 0.66
6.282GlyVal: 6.282 ± 0.619
1.688GlyTrp: 1.688 ± 0.314
2.86GlyTyr: 2.86 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
1.453HisAla: 1.453 ± 0.251
0.0HisCys: 0.0 ± 0.0
1.078HisAsp: 1.078 ± 0.222
0.938HisGlu: 0.938 ± 0.241
0.844HisPhe: 0.844 ± 0.193
1.735HisGly: 1.735 ± 0.301
0.281HisHis: 0.281 ± 0.118
1.031HisIle: 1.031 ± 0.185
0.516HisLys: 0.516 ± 0.148
1.219HisLeu: 1.219 ± 0.314
0.375HisMet: 0.375 ± 0.179
0.609HisAsn: 0.609 ± 0.204
1.453HisPro: 1.453 ± 0.295
0.844HisGln: 0.844 ± 0.211
1.969HisArg: 1.969 ± 0.337
0.938HisSer: 0.938 ± 0.216
1.125HisThr: 1.125 ± 0.191
1.453HisVal: 1.453 ± 0.279
0.375HisTrp: 0.375 ± 0.152
0.797HisTyr: 0.797 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
5.813IleAla: 5.813 ± 0.638
0.328IleCys: 0.328 ± 0.138
3.328IleAsp: 3.328 ± 0.483
4.36IleGlu: 4.36 ± 0.464
1.172IlePhe: 1.172 ± 0.224
4.032IleGly: 4.032 ± 0.656
0.656IleHis: 0.656 ± 0.16
1.969IleIle: 1.969 ± 0.297
1.594IleLys: 1.594 ± 0.314
3.797IleLeu: 3.797 ± 0.383
0.891IleMet: 0.891 ± 0.191
2.156IleAsn: 2.156 ± 0.379
2.813IlePro: 2.813 ± 0.354
1.547IleGln: 1.547 ± 0.239
2.953IleArg: 2.953 ± 0.313
2.813IleSer: 2.813 ± 0.397
3.61IleThr: 3.61 ± 0.437
3.657IleVal: 3.657 ± 0.335
0.797IleTrp: 0.797 ± 0.214
1.313IleTyr: 1.313 ± 0.271
0.0IleXaa: 0.0 ± 0.0
Lys
3.891LysAla: 3.891 ± 0.489
0.188LysCys: 0.188 ± 0.096
3.0LysAsp: 3.0 ± 0.5
1.781LysGlu: 1.781 ± 0.273
1.313LysPhe: 1.313 ± 0.249
3.328LysGly: 3.328 ± 0.462
0.609LysHis: 0.609 ± 0.169
2.297LysIle: 2.297 ± 0.397
1.594LysLys: 1.594 ± 0.392
3.469LysLeu: 3.469 ± 0.406
0.984LysMet: 0.984 ± 0.208
1.36LysAsn: 1.36 ± 0.235
2.203LysPro: 2.203 ± 0.318
1.406LysGln: 1.406 ± 0.22
3.141LysArg: 3.141 ± 0.424
1.922LysSer: 1.922 ± 0.249
2.907LysThr: 2.907 ± 0.359
2.907LysVal: 2.907 ± 0.353
0.75LysTrp: 0.75 ± 0.147
1.031LysTyr: 1.031 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
8.579LeuAla: 8.579 ± 0.743
0.891LeuCys: 0.891 ± 0.252
5.204LeuAsp: 5.204 ± 0.503
4.313LeuGlu: 4.313 ± 0.506
1.922LeuPhe: 1.922 ± 0.317
6.188LeuGly: 6.188 ± 0.758
1.5LeuHis: 1.5 ± 0.222
3.094LeuIle: 3.094 ± 0.373
2.907LeuLys: 2.907 ± 0.403
5.297LeuLeu: 5.297 ± 0.517
1.594LeuMet: 1.594 ± 0.222
2.344LeuAsn: 2.344 ± 0.324
4.079LeuPro: 4.079 ± 0.431
2.11LeuGln: 2.11 ± 0.271
6.985LeuArg: 6.985 ± 0.583
4.079LeuSer: 4.079 ± 0.398
5.344LeuThr: 5.344 ± 0.503
4.735LeuVal: 4.735 ± 0.487
1.5LeuTrp: 1.5 ± 0.286
1.875LeuTyr: 1.875 ± 0.287
0.0LeuXaa: 0.0 ± 0.0
Met
2.11MetAla: 2.11 ± 0.43
0.047MetCys: 0.047 ± 0.043
1.266MetAsp: 1.266 ± 0.239
1.5MetGlu: 1.5 ± 0.263
0.75MetPhe: 0.75 ± 0.215
1.313MetGly: 1.313 ± 0.234
0.281MetHis: 0.281 ± 0.132
0.797MetIle: 0.797 ± 0.212
1.125MetLys: 1.125 ± 0.217
1.406MetLeu: 1.406 ± 0.29
0.375MetMet: 0.375 ± 0.141
0.984MetAsn: 0.984 ± 0.192
1.781MetPro: 1.781 ± 0.321
0.563MetGln: 0.563 ± 0.166
1.828MetArg: 1.828 ± 0.272
1.688MetSer: 1.688 ± 0.291
2.344MetThr: 2.344 ± 0.362
1.266MetVal: 1.266 ± 0.23
0.469MetTrp: 0.469 ± 0.146
0.469MetTyr: 0.469 ± 0.138
0.0MetXaa: 0.0 ± 0.0
Asn
3.0AsnAla: 3.0 ± 0.377
0.234AsnCys: 0.234 ± 0.091
2.063AsnAsp: 2.063 ± 0.387
2.11AsnGlu: 2.11 ± 0.257
1.078AsnPhe: 1.078 ± 0.197
3.704AsnGly: 3.704 ± 0.435
0.609AsnHis: 0.609 ± 0.171
1.406AsnIle: 1.406 ± 0.296
0.797AsnLys: 0.797 ± 0.219
2.063AsnLeu: 2.063 ± 0.266
0.609AsnMet: 0.609 ± 0.177
1.078AsnAsn: 1.078 ± 0.244
2.766AsnPro: 2.766 ± 0.373
1.078AsnGln: 1.078 ± 0.183
2.11AsnArg: 2.11 ± 0.261
1.547AsnSer: 1.547 ± 0.267
2.297AsnThr: 2.297 ± 0.304
2.11AsnVal: 2.11 ± 0.285
0.891AsnTrp: 0.891 ± 0.167
0.938AsnTyr: 0.938 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
5.532ProAla: 5.532 ± 0.546
0.375ProCys: 0.375 ± 0.172
4.547ProAsp: 4.547 ± 0.468
3.704ProGlu: 3.704 ± 0.531
1.688ProPhe: 1.688 ± 0.254
4.876ProGly: 4.876 ± 0.452
0.891ProHis: 0.891 ± 0.188
3.375ProIle: 3.375 ± 0.49
2.907ProLys: 2.907 ± 0.424
4.219ProLeu: 4.219 ± 0.444
1.547ProMet: 1.547 ± 0.233
1.688ProAsn: 1.688 ± 0.287
2.907ProPro: 2.907 ± 0.393
1.594ProGln: 1.594 ± 0.26
4.079ProArg: 4.079 ± 0.464
2.766ProSer: 2.766 ± 0.272
3.188ProThr: 3.188 ± 0.393
3.938ProVal: 3.938 ± 0.508
0.797ProTrp: 0.797 ± 0.178
0.984ProTyr: 0.984 ± 0.204
0.0ProXaa: 0.0 ± 0.0
Gln
3.75GlnAla: 3.75 ± 0.526
0.328GlnCys: 0.328 ± 0.149
1.406GlnAsp: 1.406 ± 0.249
2.156GlnGlu: 2.156 ± 0.32
1.078GlnPhe: 1.078 ± 0.184
2.25GlnGly: 2.25 ± 0.325
0.141GlnHis: 0.141 ± 0.074
2.063GlnIle: 2.063 ± 0.361
1.078GlnLys: 1.078 ± 0.214
2.297GlnLeu: 2.297 ± 0.314
0.984GlnMet: 0.984 ± 0.166
1.313GlnAsn: 1.313 ± 0.246
1.5GlnPro: 1.5 ± 0.288
1.406GlnGln: 1.406 ± 0.323
3.094GlnArg: 3.094 ± 0.445
1.5GlnSer: 1.5 ± 0.309
1.875GlnThr: 1.875 ± 0.299
2.344GlnVal: 2.344 ± 0.357
0.844GlnTrp: 0.844 ± 0.231
0.797GlnTyr: 0.797 ± 0.165
0.0GlnXaa: 0.0 ± 0.0
Arg
7.36ArgAla: 7.36 ± 0.575
0.797ArgCys: 0.797 ± 0.205
5.813ArgAsp: 5.813 ± 0.629
5.438ArgGlu: 5.438 ± 0.663
2.672ArgPhe: 2.672 ± 0.451
6.141ArgGly: 6.141 ± 0.533
1.5ArgHis: 1.5 ± 0.312
3.188ArgIle: 3.188 ± 0.384
3.985ArgLys: 3.985 ± 0.433
5.297ArgLeu: 5.297 ± 0.514
2.11ArgMet: 2.11 ± 0.36
2.156ArgAsn: 2.156 ± 0.328
3.563ArgPro: 3.563 ± 0.381
2.063ArgGln: 2.063 ± 0.32
7.126ArgArg: 7.126 ± 0.793
3.047ArgSer: 3.047 ± 0.442
3.891ArgThr: 3.891 ± 0.42
4.829ArgVal: 4.829 ± 0.453
1.5ArgTrp: 1.5 ± 0.224
1.781ArgTyr: 1.781 ± 0.279
0.0ArgXaa: 0.0 ± 0.0
Ser
5.391SerAla: 5.391 ± 0.522
0.188SerCys: 0.188 ± 0.091
3.704SerAsp: 3.704 ± 0.455
3.0SerGlu: 3.0 ± 0.403
1.547SerPhe: 1.547 ± 0.309
5.532SerGly: 5.532 ± 0.544
0.844SerHis: 0.844 ± 0.212
2.203SerIle: 2.203 ± 0.262
1.875SerLys: 1.875 ± 0.285
3.047SerLeu: 3.047 ± 0.374
1.641SerMet: 1.641 ± 0.284
1.735SerAsn: 1.735 ± 0.319
2.672SerPro: 2.672 ± 0.364
2.063SerGln: 2.063 ± 0.263
3.516SerArg: 3.516 ± 0.394
3.0SerSer: 3.0 ± 0.52
3.422SerThr: 3.422 ± 0.419
4.219SerVal: 4.219 ± 0.397
0.703SerTrp: 0.703 ± 0.166
0.703SerTyr: 0.703 ± 0.203
0.0SerXaa: 0.0 ± 0.0
Thr
7.548ThrAla: 7.548 ± 0.766
0.375ThrCys: 0.375 ± 0.152
3.891ThrAsp: 3.891 ± 0.377
3.235ThrGlu: 3.235 ± 0.367
2.672ThrPhe: 2.672 ± 0.381
7.126ThrGly: 7.126 ± 0.743
1.078ThrHis: 1.078 ± 0.215
3.891ThrIle: 3.891 ± 0.399
3.0ThrLys: 3.0 ± 0.399
4.5ThrLeu: 4.5 ± 0.502
1.031ThrMet: 1.031 ± 0.223
1.922ThrAsn: 1.922 ± 0.268
4.5ThrPro: 4.5 ± 0.413
1.547ThrGln: 1.547 ± 0.243
4.36ThrArg: 4.36 ± 0.434
3.094ThrSer: 3.094 ± 0.361
4.547ThrThr: 4.547 ± 0.461
4.782ThrVal: 4.782 ± 0.602
1.453ThrTrp: 1.453 ± 0.286
1.406ThrTyr: 1.406 ± 0.257
0.0ThrXaa: 0.0 ± 0.0
Val
6.844ValAla: 6.844 ± 0.646
0.422ValCys: 0.422 ± 0.15
5.063ValAsp: 5.063 ± 0.459
5.344ValGlu: 5.344 ± 0.481
2.063ValPhe: 2.063 ± 0.283
6.469ValGly: 6.469 ± 0.693
1.36ValHis: 1.36 ± 0.302
3.469ValIle: 3.469 ± 0.509
2.344ValLys: 2.344 ± 0.292
4.735ValLeu: 4.735 ± 0.468
0.891ValMet: 0.891 ± 0.215
2.063ValAsn: 2.063 ± 0.244
3.704ValPro: 3.704 ± 0.411
2.063ValGln: 2.063 ± 0.38
5.157ValArg: 5.157 ± 0.579
3.657ValSer: 3.657 ± 0.406
5.954ValThr: 5.954 ± 0.572
5.157ValVal: 5.157 ± 0.561
0.984ValTrp: 0.984 ± 0.239
1.5ValTyr: 1.5 ± 0.316
0.0ValXaa: 0.0 ± 0.0
Trp
1.688TrpAla: 1.688 ± 0.339
0.094TrpCys: 0.094 ± 0.065
1.5TrpAsp: 1.5 ± 0.255
1.313TrpGlu: 1.313 ± 0.25
0.422TrpPhe: 0.422 ± 0.121
1.266TrpGly: 1.266 ± 0.254
0.281TrpHis: 0.281 ± 0.124
1.078TrpIle: 1.078 ± 0.229
0.797TrpLys: 0.797 ± 0.216
1.875TrpLeu: 1.875 ± 0.296
0.375TrpMet: 0.375 ± 0.161
0.563TrpAsn: 0.563 ± 0.155
0.516TrpPro: 0.516 ± 0.162
0.609TrpGln: 0.609 ± 0.209
1.453TrpArg: 1.453 ± 0.288
1.172TrpSer: 1.172 ± 0.198
1.453TrpThr: 1.453 ± 0.293
1.5TrpVal: 1.5 ± 0.231
0.938TrpTrp: 0.938 ± 0.18
0.516TrpTyr: 0.516 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.922TyrAla: 1.922 ± 0.258
0.281TyrCys: 0.281 ± 0.108
1.641TyrAsp: 1.641 ± 0.268
1.875TyrGlu: 1.875 ± 0.316
0.797TyrPhe: 0.797 ± 0.176
2.907TyrGly: 2.907 ± 0.489
0.516TyrHis: 0.516 ± 0.182
1.266TyrIle: 1.266 ± 0.266
0.563TyrLys: 0.563 ± 0.147
2.25TyrLeu: 2.25 ± 0.354
0.516TyrMet: 0.516 ± 0.16
0.516TyrAsn: 0.516 ± 0.147
2.156TyrPro: 2.156 ± 0.387
0.516TyrGln: 0.516 ± 0.138
2.25TyrArg: 2.25 ± 0.349
1.313TyrSer: 1.313 ± 0.325
1.453TyrThr: 1.453 ± 0.266
1.453TyrVal: 1.453 ± 0.28
0.609TyrTrp: 0.609 ± 0.189
0.563TyrTyr: 0.563 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (21332 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski