Streptococcus phage Javan481
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AD07|A0A4D6AD07_9CAUD Cro-like phage transcriptional repressor protein OS=Streptococcus phage Javan481 OX=2548197 GN=Javan481_0004 PE=4 SV=1
MM1 pKa = 7.45 IKK3 pKa = 10.1 LVKK6 pKa = 10.15 IEE8 pKa = 4.35 GYY10 pKa = 9.98 YY11 pKa = 10.08 INPEE15 pKa = 4.04 YY16 pKa = 10.95 VVGVWEE22 pKa = 4.5 RR23 pKa = 11.84 SALNFDD29 pKa = 3.95 NLDD32 pKa = 3.51 EE33 pKa = 4.54 KK34 pKa = 11.33 VVVVQFVGDD43 pKa = 3.58 RR44 pKa = 11.84 EE45 pKa = 3.99 RR46 pKa = 11.84 EE47 pKa = 3.93 EE48 pKa = 5.61 FVFCDD53 pKa = 3.68 TPIDD57 pKa = 3.95 EE58 pKa = 4.73 VIKK61 pKa = 10.86 RR62 pKa = 11.84 LLDD65 pKa = 3.79 DD66 pKa = 3.91
Molecular weight: 7.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.452
IPC2_protein 4.355
IPC_protein 4.228
Toseland 4.05
ProMoST 4.253
Dawson 4.19
Bjellqvist 4.393
Wikipedia 4.075
Rodwell 4.062
Grimsley 3.973
Solomon 4.177
Lehninger 4.126
Nozaki 4.304
DTASelect 4.457
Thurlkill 4.088
EMBOSS 4.088
Sillero 4.342
Patrickios 3.859
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.27
Protein with the highest isoelectric point:
>tr|A0A4D6AGA3|A0A4D6AGA3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan481 OX=2548197 GN=Javan481_0016 PE=4 SV=1
MM1 pKa = 7.4 NKK3 pKa = 10.29 LEE5 pKa = 4.69 LFLLATTVILAVIARR20 pKa = 11.84 VQHH23 pKa = 5.44 EE24 pKa = 4.77 VIKK27 pKa = 10.66 KK28 pKa = 9.96 HH29 pKa = 5.97 NSPEE33 pKa = 3.91 NKK35 pKa = 9.18 RR36 pKa = 11.84 KK37 pKa = 9.67 IFRR40 pKa = 11.84 EE41 pKa = 4.02 VALKK45 pKa = 10.59 NSKK48 pKa = 9.33 GWSEE52 pKa = 3.45 KK53 pKa = 9.14 RR54 pKa = 11.84 ARR56 pKa = 11.84 GGVVSS61 pKa = 4.31
Molecular weight: 6.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 9.692
IPC_protein 9.926
Toseland 11.082
ProMoST 10.613
Dawson 11.111
Bjellqvist 10.73
Wikipedia 11.257
Rodwell 11.564
Grimsley 11.125
Solomon 11.242
Lehninger 11.213
Nozaki 11.038
DTASelect 10.73
Thurlkill 11.038
EMBOSS 11.462
Sillero 11.038
Patrickios 11.33
IPC_peptide 11.257
IPC2_peptide 9.048
IPC2.peptide.svr19 8.814
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
10465
59
785
205.2
22.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.606 ± 0.559
0.669 ± 0.11
6.651 ± 0.334
7.004 ± 0.555
4.032 ± 0.222
6.613 ± 0.425
1.414 ± 0.153
7.09 ± 0.217
9.135 ± 0.425
8.122 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.456 ± 0.163
5.447 ± 0.285
2.848 ± 0.251
3.631 ± 0.202
3.765 ± 0.236
6.718 ± 0.449
5.905 ± 0.259
6.249 ± 0.227
1.128 ± 0.127
3.516 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here