Capybara microvirus Cap1_SP_147
Average proteome isoelectric point is 5.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4E6|A0A4P8W4E6_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_147 OX=2585391 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.63 YY3 pKa = 9.96 EE4 pKa = 4.08 VYY6 pKa = 10.39 AVKK9 pKa = 10.71 DD10 pKa = 3.76 SITGFNQPACEE21 pKa = 4.06 LNQAAALRR29 pKa = 11.84 SFKK32 pKa = 11.02 NLINLNEE39 pKa = 4.07 AFRR42 pKa = 11.84 INAGDD47 pKa = 3.53 YY48 pKa = 10.97 SLFKK52 pKa = 10.65 IADD55 pKa = 3.56 FDD57 pKa = 4.3 TDD59 pKa = 2.93 TGYY62 pKa = 8.66 CTGIDD67 pKa = 3.35 PVFIVSGSSVLEE79 pKa = 4.1 VNNAA83 pKa = 3.15
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.29
IPC2_protein 4.469
IPC_protein 4.279
Toseland 4.101
ProMoST 4.431
Dawson 4.253
Bjellqvist 4.418
Wikipedia 4.19
Rodwell 4.113
Grimsley 4.012
Solomon 4.24
Lehninger 4.202
Nozaki 4.38
DTASelect 4.584
Thurlkill 4.139
EMBOSS 4.19
Sillero 4.406
Patrickios 3.198
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.342
Protein with the highest isoelectric point:
>tr|A0A4P8W7Z9|A0A4P8W7Z9_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_147 OX=2585391 PE=3 SV=1
MM1 pKa = 7.73 CKK3 pKa = 10.08 HH4 pKa = 6.35 PLKK7 pKa = 10.53 AFQYY11 pKa = 9.95 GLHH14 pKa = 6.33 EE15 pKa = 4.78 KK16 pKa = 10.16 SGKK19 pKa = 8.64 PNYY22 pKa = 9.77 IIAPYY27 pKa = 7.32 QCKK30 pKa = 10.66 YY31 pKa = 10.29 IDD33 pKa = 3.79 VDD35 pKa = 3.36 IHH37 pKa = 6.73 DD38 pKa = 5.2 VPHH41 pKa = 6.64 KK42 pKa = 10.09 MYY44 pKa = 10.54 NEE46 pKa = 4.05 PVRR49 pKa = 11.84 SQLTKK54 pKa = 10.82 SIITSFLEE62 pKa = 4.32 IPCGKK67 pKa = 10.18 CIEE70 pKa = 4.44 CRR72 pKa = 11.84 LTYY75 pKa = 10.36 ARR77 pKa = 11.84 QWANRR82 pKa = 11.84 CQVEE86 pKa = 3.66 ADD88 pKa = 3.53 QYY90 pKa = 11.37 EE91 pKa = 4.56 KK92 pKa = 11.36 NCFITLTYY100 pKa = 10.4 SDD102 pKa = 5.44 DD103 pKa = 4.14 YY104 pKa = 11.68 IHH106 pKa = 7.04 FNTFTDD112 pKa = 4.0 PVTGEE117 pKa = 4.19 LKK119 pKa = 10.61 KK120 pKa = 11.11 SPTLVKK126 pKa = 10.24 RR127 pKa = 11.84 DD128 pKa = 3.16 IQNFMKK134 pKa = 10.71 RR135 pKa = 11.84 LRR137 pKa = 11.84 KK138 pKa = 9.81 KK139 pKa = 8.52 YY140 pKa = 9.3 GAGIRR145 pKa = 11.84 FFACGEE151 pKa = 4.14 YY152 pKa = 11.08 GEE154 pKa = 4.73 TTHH157 pKa = 6.99 RR158 pKa = 11.84 PHH160 pKa = 6.02 YY161 pKa = 10.39 HH162 pKa = 7.1 LILFNHH168 pKa = 7.03 DD169 pKa = 3.71 FNDD172 pKa = 3.33 KK173 pKa = 10.78 KK174 pKa = 10.81 LFKK177 pKa = 10.45 VSRR180 pKa = 11.84 GNPYY184 pKa = 10.26 YY185 pKa = 10.18 ISDD188 pKa = 4.26 EE189 pKa = 5.06 LSDD192 pKa = 3.64 LWPFGFSMIGDD203 pKa = 3.97 VSWQSCNYY211 pKa = 7.18 VARR214 pKa = 11.84 YY215 pKa = 6.75 ITKK218 pKa = 9.87 KK219 pKa = 10.12 QYY221 pKa = 10.32 KK222 pKa = 9.23 KK223 pKa = 10.73 KK224 pKa = 10.56 NEE226 pKa = 3.96 EE227 pKa = 3.95 KK228 pKa = 10.78 DD229 pKa = 3.84 CIIYY233 pKa = 10.37 NYY235 pKa = 9.9 EE236 pKa = 3.9 PEE238 pKa = 4.15 FVLMSRR244 pKa = 11.84 KK245 pKa = 9.53 PGIGLKK251 pKa = 10.43 YY252 pKa = 10.97 YY253 pKa = 10.36 EE254 pKa = 5.27 DD255 pKa = 3.62 NKK257 pKa = 10.73 QDD259 pKa = 4.59 FIRR262 pKa = 11.84 FNGVYY267 pKa = 9.53 LQNGRR272 pKa = 11.84 LFTPPRR278 pKa = 11.84 YY279 pKa = 9.29 FNKK282 pKa = 10.3 KK283 pKa = 10.18 LEE285 pKa = 3.98 IDD287 pKa = 3.86 FGSEE291 pKa = 3.74 YY292 pKa = 10.88 LEE294 pKa = 3.98 KK295 pKa = 10.64 KK296 pKa = 9.53 EE297 pKa = 3.9 RR298 pKa = 11.84 NKK300 pKa = 10.89 KK301 pKa = 9.42 IFNDD305 pKa = 3.38 QSILKK310 pKa = 10.61 DD311 pKa = 3.44 EE312 pKa = 4.43 LTDD315 pKa = 3.96 LNYY318 pKa = 10.74 LEE320 pKa = 4.57 RR321 pKa = 11.84 LQVEE325 pKa = 4.11 EE326 pKa = 6.02 DD327 pKa = 3.17 ILLKK331 pKa = 9.27 KK332 pKa = 9.24 TKK334 pKa = 9.69 ILHH337 pKa = 6.51 RR338 pKa = 11.84 NGADD342 pKa = 3.38 PLGVLL347 pKa = 4.63
Molecular weight: 41.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.998
IPC2_protein 8.126
IPC_protein 7.966
Toseland 8.009
ProMoST 8.507
Dawson 8.77
Bjellqvist 8.902
Wikipedia 8.799
Rodwell 8.843
Grimsley 8.024
Solomon 8.843
Lehninger 8.829
Nozaki 9.019
DTASelect 8.712
Thurlkill 8.785
EMBOSS 8.931
Sillero 9.033
Patrickios 4.126
IPC_peptide 8.829
IPC2_peptide 7.688
IPC2.peptide.svr19 7.864
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1419
83
567
283.8
31.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.906 ± 2.701
1.268 ± 0.57
5.708 ± 0.82
5.426 ± 0.625
5.004 ± 0.686
6.413 ± 0.672
2.044 ± 0.346
5.567 ± 0.545
5.99 ± 1.47
7.752 ± 0.361
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.358
7.541 ± 0.94
5.004 ± 1.156
3.946 ± 0.338
3.453 ± 0.724
7.893 ± 1.672
6.131 ± 0.691
4.933 ± 0.86
1.198 ± 0.446
5.426 ± 0.733
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here