Clostridium sp. CAG:269

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1540 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7IUR6|R7IUR6_9CLOT Stage II sporulation protein D OS=Clostridium sp. CAG:269 OX=1262788 GN=BN577_00549 PE=4 SV=1
MM1 pKa = 7.46NSCEE5 pKa = 4.3LVTLVSFLSCLISNSYY21 pKa = 11.01DD22 pKa = 3.56NEE24 pKa = 3.97EE25 pKa = 4.21LAVLAAVFTQLGDD38 pKa = 3.66SLATILANKK47 pKa = 9.86DD48 pKa = 3.69LLDD51 pKa = 4.13KK52 pKa = 10.88KK53 pKa = 11.08CPP55 pKa = 3.62

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7IUQ2|R7IUQ2_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:269 OX=1262788 GN=BN577_00652 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.14KK4 pKa = 10.19SLKK7 pKa = 10.13VKK9 pKa = 8.46QARR12 pKa = 11.84PQKK15 pKa = 9.97YY16 pKa = 7.6ATRR19 pKa = 11.84EE20 pKa = 3.81YY21 pKa = 10.63NRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 7.37AYY33 pKa = 8.2IRR35 pKa = 11.84KK36 pKa = 8.51YY37 pKa = 10.46GICRR41 pKa = 11.84VCFRR45 pKa = 11.84EE46 pKa = 4.0LAHH49 pKa = 6.83KK50 pKa = 11.06GEE52 pKa = 4.16IPGVKK57 pKa = 9.3KK58 pKa = 10.94ASWW61 pKa = 3.03

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1540

0

1540

451969

29

2683

293.5

33.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.096 ± 0.055

1.094 ± 0.021

5.448 ± 0.043

8.562 ± 0.074

3.959 ± 0.049

5.631 ± 0.053

1.172 ± 0.023

10.485 ± 0.077

10.075 ± 0.061

8.203 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.027

6.844 ± 0.066

2.432 ± 0.031

3.061 ± 0.037

3.24 ± 0.048

5.712 ± 0.054

5.444 ± 0.062

5.96 ± 0.047

0.654 ± 0.019

4.419 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski