Amino acid dipepetide frequency for Pseudonocardia thermophila

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
25.113AlaAla: 25.113 ± 0.199
1.073AlaCys: 1.073 ± 0.025
8.861AlaAsp: 8.861 ± 0.076
9.913AlaGlu: 9.913 ± 0.08
3.539AlaPhe: 3.539 ± 0.048
13.718AlaGly: 13.718 ± 0.116
2.794AlaHis: 2.794 ± 0.035
4.524AlaIle: 4.524 ± 0.06
2.324AlaLys: 2.324 ± 0.048
14.839AlaLeu: 14.839 ± 0.121
2.574AlaMet: 2.574 ± 0.037
1.845AlaAsn: 1.845 ± 0.032
7.372AlaPro: 7.372 ± 0.085
3.711AlaGln: 3.711 ± 0.051
11.084AlaArg: 11.084 ± 0.095
5.212AlaSer: 5.212 ± 0.05
7.426AlaThr: 7.426 ± 0.081
13.888AlaVal: 13.888 ± 0.105
1.779AlaTrp: 1.779 ± 0.029
2.294AlaTyr: 2.294 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.196CysAla: 1.196 ± 0.028
0.1CysCys: 0.1 ± 0.008
0.441CysAsp: 0.441 ± 0.015
0.373CysGlu: 0.373 ± 0.015
0.209CysPhe: 0.209 ± 0.011
1.008CysGly: 1.008 ± 0.025
0.173CysHis: 0.173 ± 0.009
0.228CysIle: 0.228 ± 0.011
0.087CysLys: 0.087 ± 0.008
0.66CysLeu: 0.66 ± 0.015
0.118CysMet: 0.118 ± 0.008
0.116CysAsn: 0.116 ± 0.008
0.511CysPro: 0.511 ± 0.017
0.143CysGln: 0.143 ± 0.008
0.634CysArg: 0.634 ± 0.02
0.479CysSer: 0.479 ± 0.023
0.55CysThr: 0.55 ± 0.022
0.647CysVal: 0.647 ± 0.021
0.12CysTrp: 0.12 ± 0.009
0.184CysTyr: 0.184 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.911AspAla: 7.911 ± 0.075
0.394AspCys: 0.394 ± 0.018
3.696AspAsp: 3.696 ± 0.048
4.293AspGlu: 4.293 ± 0.056
1.325AspPhe: 1.325 ± 0.028
6.004AspGly: 6.004 ± 0.061
1.415AspHis: 1.415 ± 0.03
1.642AspIle: 1.642 ± 0.037
0.771AspLys: 0.771 ± 0.024
6.505AspLeu: 6.505 ± 0.059
0.734AspMet: 0.734 ± 0.022
0.692AspAsn: 0.692 ± 0.019
5.377AspPro: 5.377 ± 0.062
1.379AspGln: 1.379 ± 0.03
5.184AspArg: 5.184 ± 0.052
1.82AspSer: 1.82 ± 0.033
2.705AspThr: 2.705 ± 0.04
5.97AspVal: 5.97 ± 0.067
0.83AspTrp: 0.83 ± 0.022
1.001AspTyr: 1.001 ± 0.027
0.0AspXaa: 0.0 ± 0.0
Glu
6.939GluAla: 6.939 ± 0.073
0.351GluCys: 0.351 ± 0.015
2.522GluAsp: 2.522 ± 0.039
3.348GluGlu: 3.348 ± 0.043
1.642GluPhe: 1.642 ± 0.03
3.704GluGly: 3.704 ± 0.047
1.756GluHis: 1.756 ± 0.034
2.714GluIle: 2.714 ± 0.044
1.222GluLys: 1.222 ± 0.032
7.723GluLeu: 7.723 ± 0.074
0.903GluMet: 0.903 ± 0.021
0.926GluAsn: 0.926 ± 0.022
3.884GluPro: 3.884 ± 0.051
2.431GluGln: 2.431 ± 0.034
5.842GluArg: 5.842 ± 0.064
2.157GluSer: 2.157 ± 0.034
2.58GluThr: 2.58 ± 0.036
5.08GluVal: 5.08 ± 0.062
0.858GluTrp: 0.858 ± 0.022
1.029GluTyr: 1.029 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.951PheAla: 3.951 ± 0.048
0.301PheCys: 0.301 ± 0.011
1.925PheAsp: 1.925 ± 0.033
1.278PheGlu: 1.278 ± 0.032
0.774PhePhe: 0.774 ± 0.025
3.029PheGly: 3.029 ± 0.048
0.579PheHis: 0.579 ± 0.018
0.655PheIle: 0.655 ± 0.021
0.341PheLys: 0.341 ± 0.014
2.474PheLeu: 2.474 ± 0.042
0.323PheMet: 0.323 ± 0.013
0.459PheAsn: 0.459 ± 0.017
1.381PhePro: 1.381 ± 0.028
0.53PheGln: 0.53 ± 0.019
1.832PheArg: 1.832 ± 0.029
1.265PheSer: 1.265 ± 0.027
1.927PheThr: 1.927 ± 0.036
2.493PheVal: 2.493 ± 0.038
0.4PheTrp: 0.4 ± 0.014
0.553PheTyr: 0.553 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
11.17GlyAla: 11.17 ± 0.094
0.857GlyCys: 0.857 ± 0.023
4.792GlyAsp: 4.792 ± 0.054
5.081GlyGlu: 5.081 ± 0.055
2.872GlyPhe: 2.872 ± 0.041
8.226GlyGly: 8.226 ± 0.095
2.014GlyHis: 2.014 ± 0.035
3.934GlyIle: 3.934 ± 0.05
1.877GlyLys: 1.877 ± 0.044
9.384GlyLeu: 9.384 ± 0.074
2.067GlyMet: 2.067 ± 0.039
1.442GlyAsn: 1.442 ± 0.032
5.652GlyPro: 5.652 ± 0.055
2.354GlyGln: 2.354 ± 0.043
7.849GlyArg: 7.849 ± 0.074
4.859GlySer: 4.859 ± 0.058
5.616GlyThr: 5.616 ± 0.057
8.148GlyVal: 8.148 ± 0.07
1.652GlyTrp: 1.652 ± 0.029
2.156GlyTyr: 2.156 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.669HisAla: 2.669 ± 0.045
0.205HisCys: 0.205 ± 0.01
1.351HisAsp: 1.351 ± 0.028
1.186HisGlu: 1.186 ± 0.025
0.516HisPhe: 0.516 ± 0.017
2.246HisGly: 2.246 ± 0.038
0.649HisHis: 0.649 ± 0.019
0.635HisIle: 0.635 ± 0.021
0.273HisLys: 0.273 ± 0.013
2.316HisLeu: 2.316 ± 0.036
0.306HisMet: 0.306 ± 0.014
0.352HisAsn: 0.352 ± 0.016
1.794HisPro: 1.794 ± 0.031
0.521HisGln: 0.521 ± 0.018
2.154HisArg: 2.154 ± 0.04
0.792HisSer: 0.792 ± 0.021
1.111HisThr: 1.111 ± 0.024
2.022HisVal: 2.022 ± 0.035
0.317HisTrp: 0.317 ± 0.014
0.463HisTyr: 0.463 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
5.918IleAla: 5.918 ± 0.061
0.324IleCys: 0.324 ± 0.013
2.789IleAsp: 2.789 ± 0.044
2.262IleGlu: 2.262 ± 0.034
0.712IlePhe: 0.712 ± 0.024
4.142IleGly: 4.142 ± 0.046
0.57IleHis: 0.57 ± 0.017
0.959IleIle: 0.959 ± 0.028
0.594IleLys: 0.594 ± 0.019
2.292IleLeu: 2.292 ± 0.035
0.453IleMet: 0.453 ± 0.015
0.723IleAsn: 0.723 ± 0.021
1.887IlePro: 1.887 ± 0.033
0.604IleGln: 0.604 ± 0.021
2.34IleArg: 2.34 ± 0.038
1.671IleSer: 1.671 ± 0.034
2.574IleThr: 2.574 ± 0.035
3.643IleVal: 3.643 ± 0.052
0.374IleTrp: 0.374 ± 0.017
0.573IleTyr: 0.573 ± 0.02
0.0IleXaa: 0.0 ± 0.0
Lys
2.217LysAla: 2.217 ± 0.047
0.085LysCys: 0.085 ± 0.007
0.832LysAsp: 0.832 ± 0.021
0.906LysGlu: 0.906 ± 0.027
0.403LysPhe: 0.403 ± 0.017
1.183LysGly: 1.183 ± 0.035
0.365LysHis: 0.365 ± 0.013
0.807LysIle: 0.807 ± 0.026
0.467LysLys: 0.467 ± 0.022
1.689LysLeu: 1.689 ± 0.032
0.275LysMet: 0.275 ± 0.012
0.34LysAsn: 0.34 ± 0.016
1.093LysPro: 1.093 ± 0.028
0.537LysGln: 0.537 ± 0.018
1.278LysArg: 1.278 ± 0.031
0.734LysSer: 0.734 ± 0.023
0.897LysThr: 0.897 ± 0.024
1.512LysVal: 1.512 ± 0.03
0.201LysTrp: 0.201 ± 0.012
0.321LysTyr: 0.321 ± 0.013
0.0LysXaa: 0.0 ± 0.0
Leu
16.442LeuAla: 16.442 ± 0.121
0.767LeuCys: 0.767 ± 0.022
7.009LeuAsp: 7.009 ± 0.066
4.7LeuGlu: 4.7 ± 0.065
2.431LeuPhe: 2.431 ± 0.04
9.259LeuGly: 9.259 ± 0.076
2.264LeuHis: 2.264 ± 0.038
3.256LeuIle: 3.256 ± 0.046
1.314LeuLys: 1.314 ± 0.028
11.067LeuLeu: 11.067 ± 0.12
1.491LeuMet: 1.491 ± 0.03
1.403LeuAsn: 1.403 ± 0.028
6.507LeuPro: 6.507 ± 0.067
2.42LeuGln: 2.42 ± 0.037
8.979LeuArg: 8.979 ± 0.071
4.577LeuSer: 4.577 ± 0.059
6.232LeuThr: 6.232 ± 0.054
10.394LeuVal: 10.394 ± 0.097
1.168LeuTrp: 1.168 ± 0.029
1.501LeuTyr: 1.501 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.222MetAla: 2.222 ± 0.037
0.142MetCys: 0.142 ± 0.009
0.73MetAsp: 0.73 ± 0.018
0.635MetGlu: 0.635 ± 0.02
0.434MetPhe: 0.434 ± 0.016
1.19MetGly: 1.19 ± 0.025
0.387MetHis: 0.387 ± 0.014
0.764MetIle: 0.764 ± 0.021
0.318MetLys: 0.318 ± 0.013
1.713MetLeu: 1.713 ± 0.034
0.253MetMet: 0.253 ± 0.011
0.357MetAsn: 0.357 ± 0.014
1.11MetPro: 1.11 ± 0.027
0.46MetGln: 0.46 ± 0.017
1.494MetArg: 1.494 ± 0.026
1.239MetSer: 1.239 ± 0.029
1.639MetThr: 1.639 ± 0.025
1.341MetVal: 1.341 ± 0.03
0.179MetTrp: 0.179 ± 0.01
0.242MetTyr: 0.242 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.051AsnAla: 2.051 ± 0.039
0.152AsnCys: 0.152 ± 0.009
0.755AsnAsp: 0.755 ± 0.021
0.678AsnGlu: 0.678 ± 0.017
0.427AsnPhe: 0.427 ± 0.017
1.592AsnGly: 1.592 ± 0.036
0.314AsnHis: 0.314 ± 0.013
0.58AsnIle: 0.58 ± 0.019
0.287AsnLys: 0.287 ± 0.014
1.547AsnLeu: 1.547 ± 0.026
0.237AsnMet: 0.237 ± 0.011
0.329AsnAsn: 0.329 ± 0.015
1.34AsnPro: 1.34 ± 0.026
0.397AsnGln: 0.397 ± 0.018
1.223AsnArg: 1.223 ± 0.03
0.682AsnSer: 0.682 ± 0.018
0.886AsnThr: 0.886 ± 0.024
1.325AsnVal: 1.325 ± 0.026
0.241AsnTrp: 0.241 ± 0.012
0.34AsnTyr: 0.34 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
9.392ProAla: 9.392 ± 0.1
0.342ProCys: 0.342 ± 0.013
5.076ProAsp: 5.076 ± 0.052
4.418ProGlu: 4.418 ± 0.049
1.561ProPhe: 1.561 ± 0.028
6.776ProGly: 6.776 ± 0.066
1.326ProHis: 1.326 ± 0.027
1.899ProIle: 1.899 ± 0.035
0.934ProLys: 0.934 ± 0.022
5.455ProLeu: 5.455 ± 0.057
1.072ProMet: 1.072 ± 0.025
0.962ProAsn: 0.962 ± 0.028
4.28ProPro: 4.28 ± 0.065
1.781ProGln: 1.781 ± 0.038
4.36ProArg: 4.36 ± 0.056
2.746ProSer: 2.746 ± 0.041
3.585ProThr: 3.585 ± 0.048
6.093ProVal: 6.093 ± 0.062
0.891ProTrp: 0.891 ± 0.024
1.17ProTyr: 1.17 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.336GlnAla: 3.336 ± 0.048
0.163GlnCys: 0.163 ± 0.009
1.075GlnAsp: 1.075 ± 0.028
1.316GlnGlu: 1.316 ± 0.032
0.68GlnPhe: 0.68 ± 0.019
1.78GlnGly: 1.78 ± 0.035
0.657GlnHis: 0.657 ± 0.018
1.153GlnIle: 1.153 ± 0.031
0.478GlnLys: 0.478 ± 0.018
3.272GlnLeu: 3.272 ± 0.046
0.436GlnMet: 0.436 ± 0.016
0.437GlnAsn: 0.437 ± 0.016
1.648GlnPro: 1.648 ± 0.036
1.163GlnGln: 1.163 ± 0.034
2.572GlnArg: 2.572 ± 0.039
0.965GlnSer: 0.965 ± 0.028
1.174GlnThr: 1.174 ± 0.025
2.524GlnVal: 2.524 ± 0.04
0.396GlnTrp: 0.396 ± 0.014
0.479GlnTyr: 0.479 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
11.031ArgAla: 11.031 ± 0.092
0.741ArgCys: 0.741 ± 0.024
4.24ArgAsp: 4.24 ± 0.048
4.716ArgGlu: 4.716 ± 0.052
2.563ArgPhe: 2.563 ± 0.038
6.107ArgGly: 6.107 ± 0.061
1.911ArgHis: 1.911 ± 0.031
3.662ArgIle: 3.662 ± 0.047
1.348ArgLys: 1.348 ± 0.031
8.433ArgLeu: 8.433 ± 0.087
1.899ArgMet: 1.899 ± 0.029
1.343ArgAsn: 1.343 ± 0.03
5.402ArgPro: 5.402 ± 0.064
1.951ArgGln: 1.951 ± 0.032
8.603ArgArg: 8.603 ± 0.094
4.284ArgSer: 4.284 ± 0.052
5.262ArgThr: 5.262 ± 0.055
6.603ArgVal: 6.603 ± 0.068
1.51ArgTrp: 1.51 ± 0.032
1.814ArgTyr: 1.814 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.246SerAla: 6.246 ± 0.055
0.398SerCys: 0.398 ± 0.019
2.256SerAsp: 2.256 ± 0.039
2.009SerGlu: 2.009 ± 0.035
1.309SerPhe: 1.309 ± 0.026
5.231SerGly: 5.231 ± 0.06
0.764SerHis: 0.764 ± 0.023
1.639SerIle: 1.639 ± 0.032
0.731SerLys: 0.731 ± 0.023
3.842SerLeu: 3.842 ± 0.045
1.036SerMet: 1.036 ± 0.026
0.67SerAsn: 0.67 ± 0.019
3.005SerPro: 3.005 ± 0.043
0.942SerGln: 0.942 ± 0.027
3.357SerArg: 3.357 ± 0.047
2.408SerSer: 2.408 ± 0.048
2.878SerThr: 2.878 ± 0.046
3.974SerVal: 3.974 ± 0.044
0.795SerTrp: 0.795 ± 0.022
0.861SerTyr: 0.861 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
8.636ThrAla: 8.636 ± 0.071
0.45ThrCys: 0.45 ± 0.015
3.444ThrAsp: 3.444 ± 0.045
3.207ThrGlu: 3.207 ± 0.042
1.589ThrPhe: 1.589 ± 0.032
6.411ThrGly: 6.411 ± 0.06
1.087ThrHis: 1.087 ± 0.024
2.165ThrIle: 2.165 ± 0.033
0.9ThrLys: 0.9 ± 0.024
5.062ThrLeu: 5.062 ± 0.046
0.937ThrMet: 0.937 ± 0.025
0.908ThrAsn: 0.908 ± 0.024
3.9ThrPro: 3.9 ± 0.046
1.207ThrGln: 1.207 ± 0.027
3.908ThrArg: 3.908 ± 0.044
2.912ThrSer: 2.912 ± 0.041
3.937ThrThr: 3.937 ± 0.057
6.04ThrVal: 6.04 ± 0.068
0.851ThrTrp: 0.851 ± 0.022
1.13ThrTyr: 1.13 ± 0.023
0.0ThrXaa: 0.0 ± 0.0
Val
13.373ValAla: 13.373 ± 0.109
0.782ValCys: 0.782 ± 0.021
5.936ValAsp: 5.936 ± 0.065
5.604ValGlu: 5.604 ± 0.061
2.463ValPhe: 2.463 ± 0.035
7.57ValGly: 7.57 ± 0.067
2.145ValHis: 2.145 ± 0.037
3.149ValIle: 3.149 ± 0.047
1.336ValLys: 1.336 ± 0.03
11.164ValLeu: 11.164 ± 0.097
1.24ValMet: 1.24 ± 0.029
1.491ValAsn: 1.491 ± 0.032
6.039ValPro: 6.039 ± 0.062
2.22ValGln: 2.22 ± 0.038
7.704ValArg: 7.704 ± 0.07
3.935ValSer: 3.935 ± 0.043
5.712ValThr: 5.712 ± 0.063
10.473ValVal: 10.473 ± 0.101
1.054ValTrp: 1.054 ± 0.024
1.425ValTyr: 1.425 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.657TrpAla: 1.657 ± 0.029
0.155TrpCys: 0.155 ± 0.009
0.741TrpAsp: 0.741 ± 0.021
0.654TrpGlu: 0.654 ± 0.021
0.488TrpPhe: 0.488 ± 0.017
0.975TrpGly: 0.975 ± 0.025
0.332TrpHis: 0.332 ± 0.012
0.591TrpIle: 0.591 ± 0.019
0.242TrpLys: 0.242 ± 0.012
1.718TrpLeu: 1.718 ± 0.036
0.281TrpMet: 0.281 ± 0.012
0.3TrpAsn: 0.3 ± 0.011
0.834TrpPro: 0.834 ± 0.022
0.485TrpGln: 0.485 ± 0.016
1.295TrpArg: 1.295 ± 0.032
0.785TrpSer: 0.785 ± 0.02
0.998TrpThr: 0.998 ± 0.027
1.073TrpVal: 1.073 ± 0.023
0.34TrpTrp: 0.34 ± 0.013
0.306TrpTyr: 0.306 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.411TyrAla: 2.411 ± 0.039
0.194TyrCys: 0.194 ± 0.011
1.177TyrAsp: 1.177 ± 0.028
1.034TyrGlu: 1.034 ± 0.023
0.533TyrPhe: 0.533 ± 0.019
1.85TyrGly: 1.85 ± 0.039
0.377TyrHis: 0.377 ± 0.016
0.447TyrIle: 0.447 ± 0.017
0.297TyrLys: 0.297 ± 0.016
2.029TyrLeu: 2.029 ± 0.036
0.215TyrMet: 0.215 ± 0.011
0.289TyrAsn: 0.289 ± 0.013
1.102TyrPro: 1.102 ± 0.026
0.474TyrGln: 0.474 ± 0.015
1.73TyrArg: 1.73 ± 0.036
0.827TyrSer: 0.827 ± 0.022
0.991TyrThr: 0.991 ± 0.023
1.57TyrVal: 1.57 ± 0.032
0.294TyrTrp: 0.294 ± 0.013
0.395TyrTyr: 0.395 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5850 proteins (1837469 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski