Abaca bunchy top virus
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B0LBX8|B0LBX8_9VIRU Putative replicase protein OS=Abaca bunchy top virus OX=438782 PE=3 SV=1
MM1 pKa = 7.36 EE2 pKa = 5.25 FWNSEE7 pKa = 4.15 AFCDD11 pKa = 4.4 DD12 pKa = 3.3 VKK14 pKa = 11.06 RR15 pKa = 11.84 VIKK18 pKa = 9.97 QKK20 pKa = 9.88 YY21 pKa = 6.47 WEE23 pKa = 4.24 EE24 pKa = 3.88 RR25 pKa = 11.84 MKK27 pKa = 11.05 SLFIEE32 pKa = 4.4 KK33 pKa = 9.75 VSGYY37 pKa = 7.39 VRR39 pKa = 11.84 RR40 pKa = 11.84 ILVYY44 pKa = 10.93 GNLDD48 pKa = 3.26 DD49 pKa = 5.1 TIYY52 pKa = 11.05 AVQQMKK58 pKa = 10.02 TSIVQCAEE66 pKa = 4.07 RR67 pKa = 11.84 FGKK70 pKa = 10.63 ACVVVYY76 pKa = 10.9 NGLDD80 pKa = 3.29 PSIGFRR86 pKa = 11.84 LHH88 pKa = 5.12 TMAFFFEE95 pKa = 4.89 EE96 pKa = 4.44 YY97 pKa = 10.51 VEE99 pKa = 4.41 EE100 pKa = 4.6 VSTADD105 pKa = 3.39 PMAVQLFCDD114 pKa = 3.76 EE115 pKa = 4.73 EE116 pKa = 4.52 IEE118 pKa = 4.34 EE119 pKa = 4.45 FSNSDD124 pKa = 2.72 VRR126 pKa = 11.84 LIKK129 pKa = 10.48 NVIMASTDD137 pKa = 2.98 ASIDD141 pKa = 3.42 VGNCIQIIISDD152 pKa = 4.01 NVITFYY158 pKa = 10.64 ICC160 pKa = 3.93
Molecular weight: 18.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.421
IPC2_protein 4.546
IPC_protein 4.444
Toseland 4.279
ProMoST 4.546
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.266
Rodwell 4.279
Grimsley 4.19
Solomon 4.38
Lehninger 4.342
Nozaki 4.495
DTASelect 4.647
Thurlkill 4.291
EMBOSS 4.279
Sillero 4.558
Patrickios 3.312
IPC_peptide 4.393
IPC2_peptide 4.546
IPC2.peptide.svr19 4.495
Protein with the highest isoelectric point:
>tr|B0LBX6|B0LBX6_9VIRU Putative movement protein OS=Abaca bunchy top virus OX=438782 PE=4 SV=1
MM1 pKa = 7.47 ARR3 pKa = 11.84 YY4 pKa = 7.86 PKK6 pKa = 10.25 KK7 pKa = 10.47 ALKK10 pKa = 9.65 KK11 pKa = 9.15 RR12 pKa = 11.84 KK13 pKa = 9.18 AVRR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 9.05 YY19 pKa = 10.53 GSKK22 pKa = 9.24 ATTSHH27 pKa = 7.45 DD28 pKa = 3.87 YY29 pKa = 11.28 AVDD32 pKa = 3.22 TSFIVPEE39 pKa = 4.09 NTIKK43 pKa = 10.84 LYY45 pKa = 10.62 RR46 pKa = 11.84 IEE48 pKa = 4.53 PTDD51 pKa = 3.32 KK52 pKa = 10.0 TLPRR56 pKa = 11.84 YY57 pKa = 8.25 FIWKK61 pKa = 8.52 MFMLLVCKK69 pKa = 10.16 VRR71 pKa = 11.84 PGRR74 pKa = 11.84 ILHH77 pKa = 5.9 WAMIKK82 pKa = 10.53 SSWDD86 pKa = 3.3 VKK88 pKa = 11.19 DD89 pKa = 3.33 PTVVLEE95 pKa = 4.3 APGLFIKK102 pKa = 9.86 PANSHH107 pKa = 5.5 LVKK110 pKa = 10.42 LVCSGEE116 pKa = 4.02 LEE118 pKa = 4.2 APVGGGTSEE127 pKa = 4.26 VEE129 pKa = 3.73 CLLRR133 pKa = 11.84 KK134 pKa = 6.69 TTLLRR139 pKa = 11.84 RR140 pKa = 11.84 NVTEE144 pKa = 5.47 LDD146 pKa = 3.4 FLYY149 pKa = 10.93 LAFYY153 pKa = 10.02 CSSGVTINYY162 pKa = 7.97 QNRR165 pKa = 11.84 ITYY168 pKa = 9.37 HH169 pKa = 4.27 VV170 pKa = 3.46
Molecular weight: 19.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.152
IPC2_protein 9.238
IPC_protein 9.194
Toseland 9.853
ProMoST 9.575
Dawson 10.087
Bjellqvist 9.78
Wikipedia 10.262
Rodwell 10.496
Grimsley 10.16
Solomon 10.116
Lehninger 10.072
Nozaki 9.911
DTASelect 9.765
Thurlkill 9.94
EMBOSS 10.277
Sillero 10.014
Patrickios 9.75
IPC_peptide 10.116
IPC2_peptide 8.521
IPC2.peptide.svr19 8.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
886
116
286
177.2
20.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.079 ± 0.547
3.047 ± 0.538
4.74 ± 0.759
7.336 ± 1.159
4.628 ± 0.558
6.095 ± 0.716
1.58 ± 0.251
7.336 ± 0.758
5.982 ± 0.925
6.321 ± 1.205
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.499 ± 0.39
4.176 ± 0.737
3.95 ± 0.673
3.273 ± 0.716
6.659 ± 0.548
5.643 ± 0.989
5.305 ± 0.769
8.126 ± 0.752
1.58 ± 0.102
5.53 ± 0.432
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here