Serratia phage 2050HW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Moabitevirus; Serratia virus 2050HW

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 363 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A289Z833|A0A289Z833_9CAUD Uncharacterized protein OS=Serratia phage 2050HW OX=2024252 GN=2050HW_00360 PE=4 SV=1
MM1 pKa = 7.28SRR3 pKa = 11.84EE4 pKa = 4.27SIVSVTLKK12 pKa = 10.63SEE14 pKa = 4.11YY15 pKa = 10.4CDD17 pKa = 3.38VSEE20 pKa = 5.93KK21 pKa = 10.67EE22 pKa = 3.62FSYY25 pKa = 11.25YY26 pKa = 10.35CGEE29 pKa = 4.22EE30 pKa = 3.81RR31 pKa = 11.84SLMCVADD38 pKa = 6.14EE39 pKa = 3.71IAEE42 pKa = 4.28WIEE45 pKa = 3.99EE46 pKa = 4.42CSVMLCPEE54 pKa = 4.52PDD56 pKa = 5.13DD57 pKa = 3.57IQEE60 pKa = 4.28ALEE63 pKa = 3.77NLYY66 pKa = 11.24LNGSSKK72 pKa = 10.25MDD74 pKa = 3.55TLDD77 pKa = 3.16EE78 pKa = 4.47TEE80 pKa = 3.98VLFRR84 pKa = 11.84KK85 pKa = 9.35VV86 pKa = 2.79

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A289YZA7|A0A289YZA7_9CAUD Uncharacterized protein OS=Serratia phage 2050HW OX=2024252 GN=2050HW_00244 PE=4 SV=1
MM1 pKa = 7.56SNRR4 pKa = 11.84TRR6 pKa = 11.84AFRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 9.45KK12 pKa = 10.61RR13 pKa = 11.84EE14 pKa = 3.78LKK16 pKa = 9.4KK17 pKa = 10.57QAVRR21 pKa = 11.84RR22 pKa = 11.84YY23 pKa = 9.28GVAGYY28 pKa = 8.65WFNGDD33 pKa = 3.57NPVSDD38 pKa = 3.51KK39 pKa = 11.4VVIGKK44 pKa = 8.93VATTPKK50 pKa = 10.61ACSCWMCANHH60 pKa = 6.33GKK62 pKa = 9.86VFGEE66 pKa = 4.09RR67 pKa = 11.84FSDD70 pKa = 3.29TRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.58LKK76 pKa = 9.35HH77 pKa = 5.95TGGEE81 pKa = 4.03

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

363

0

363

84274

20

2213

232.2

26.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.038 ± 0.186

0.94 ± 0.044

6.37 ± 0.102

6.353 ± 0.168

4.386 ± 0.095

6.758 ± 0.152

1.647 ± 0.063

6.367 ± 0.09

5.835 ± 0.119

8.237 ± 0.11

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.064

5.475 ± 0.118

3.981 ± 0.082

3.089 ± 0.071

5.437 ± 0.107

6.583 ± 0.105

6.323 ± 0.105

7.223 ± 0.116

1.292 ± 0.042

3.967 ± 0.093
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski