TM7 phylum sp. oral taxon 348
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 855 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563CR36|A0A563CR36_9BACT Uncharacterized protein OS=TM7 phylum sp. oral taxon 348 OX=671231 GN=EUA62_01715 PE=4 SV=1
MM1 pKa = 6.77 STSSIYY7 pKa = 9.26 YY8 pKa = 7.82 TATDD12 pKa = 3.08 VPMRR16 pKa = 11.84 NEE18 pKa = 3.62 SWHH21 pKa = 6.63 AEE23 pKa = 4.01 QAFKK27 pKa = 10.71 SYY29 pKa = 11.33 KK30 pKa = 10.36 LGGEE34 pKa = 4.04 LWFSCVLYY42 pKa = 10.43 IVTACVGFVGLFAIDD57 pKa = 5.17 SSDD60 pKa = 3.48 CPNIVLEE67 pKa = 4.5 IFAKK71 pKa = 10.17 IYY73 pKa = 8.94 FASMCMLGIGYY84 pKa = 9.2 LAFAAKK90 pKa = 10.18 RR91 pKa = 11.84 LMYY94 pKa = 9.99 RR95 pKa = 11.84 KK96 pKa = 9.81 YY97 pKa = 9.9 YY98 pKa = 10.36 SKK100 pKa = 10.41 PSRR103 pKa = 11.84 GKK105 pKa = 10.4 GEE107 pKa = 4.58 LYY109 pKa = 10.24 RR110 pKa = 11.84 IDD112 pKa = 4.15 IIEE115 pKa = 4.9 PNDD118 pKa = 4.42 DD119 pKa = 3.15 DD120 pKa = 4.11 WGIQFTSPNGVFTYY134 pKa = 11.04 LNLGCDD140 pKa = 4.75 LEE142 pKa = 4.9 CSMYY146 pKa = 10.65 AIEE149 pKa = 5.56 LLQFDD154 pKa = 4.1 ADD156 pKa = 3.94 FRR158 pKa = 11.84 SEE160 pKa = 4.08 INSYY164 pKa = 10.54 LADD167 pKa = 3.86 PVNKK171 pKa = 9.87 KK172 pKa = 10.21 VSTEE176 pKa = 3.54 EE177 pKa = 4.15 MINSKK182 pKa = 11.02 YY183 pKa = 10.74 EE184 pKa = 4.01 DD185 pKa = 3.84 LCDD188 pKa = 4.2 DD189 pKa = 4.49 RR190 pKa = 11.84 PSEE193 pKa = 5.9 DD194 pKa = 3.71 EE195 pKa = 5.26 DD196 pKa = 4.36 EE197 pKa = 5.15 DD198 pKa = 3.91 DD199 pKa = 5.34 GRR201 pKa = 11.84 YY202 pKa = 9.91 VDD204 pKa = 5.44 DD205 pKa = 5.1 KK206 pKa = 11.42 FVV208 pKa = 2.96
Molecular weight: 23.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.019
IPC2_protein 4.279
IPC_protein 4.24
Toseland 4.037
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.113
Rodwell 4.062
Grimsley 3.948
Solomon 4.202
Lehninger 4.151
Nozaki 4.317
DTASelect 4.52
Thurlkill 4.075
EMBOSS 4.126
Sillero 4.355
Patrickios 1.011
IPC_peptide 4.202
IPC2_peptide 4.329
IPC2.peptide.svr19 4.239
Protein with the highest isoelectric point:
>tr|A0A563CML3|A0A563CML3_9BACT Transcriptional repressor NrdR OS=TM7 phylum sp. oral taxon 348 OX=671231 GN=nrdR PE=3 SV=1
MM1 pKa = 8.03 PKK3 pKa = 8.98 RR4 pKa = 11.84 TYY6 pKa = 10.09 QPHH9 pKa = 5.49 KK10 pKa = 9.24 RR11 pKa = 11.84 SRR13 pKa = 11.84 AKK15 pKa = 10.04 VHH17 pKa = 5.81 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSSRR26 pKa = 11.84 AGRR29 pKa = 11.84 LVLKK33 pKa = 10.41 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 NKK38 pKa = 9.64 KK39 pKa = 8.43 RR40 pKa = 11.84 AKK42 pKa = 9.93 IAII45 pKa = 4.09
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
855
0
855
263325
38
1286
308.0
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.522 ± 0.086
0.697 ± 0.024
5.968 ± 0.077
5.941 ± 0.082
3.799 ± 0.063
6.459 ± 0.071
1.814 ± 0.035
8.192 ± 0.091
7.104 ± 0.083
9.131 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.305 ± 0.034
5.261 ± 0.073
3.713 ± 0.07
3.671 ± 0.055
5.124 ± 0.069
7.068 ± 0.091
5.259 ± 0.056
6.521 ± 0.069
0.96 ± 0.032
3.491 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here