Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3859 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H3CAZ9|A0A0H3CAZ9_CAUVN Tetratricopeptide repeat-containing 2OG-Fe(II) oxygenase OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565050 GN=CCNA_02909 PE=4 SV=1
MM1 pKa = 7.15 SAEE4 pKa = 4.13 PFKK7 pKa = 10.98 VWQCRR12 pKa = 11.84 TCGYY16 pKa = 10.22 IYY18 pKa = 10.38 DD19 pKa = 4.58 EE20 pKa = 4.41 EE21 pKa = 4.52 QGDD24 pKa = 4.14 PGEE27 pKa = 4.38 GLAPGTRR34 pKa = 11.84 WADD37 pKa = 3.25 IPAGWVCPLCGTPKK51 pKa = 10.44 SDD53 pKa = 3.9 FDD55 pKa = 4.21 MIEE58 pKa = 3.7 LL59 pKa = 3.99
Molecular weight: 6.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.868
IPC2_protein 4.126
IPC_protein 3.961
Toseland 3.783
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.808
Grimsley 3.706
Solomon 3.923
Lehninger 3.872
Nozaki 4.075
DTASelect 4.24
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.088
Patrickios 0.693
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.966
Protein with the highest isoelectric point:
>tr|A0A0H3CB03|A0A0H3CB03_CAUVN Fumarylacetoacetase superfamily protein OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565050 GN=CCNA_02614 PE=4 SV=1
MM1 pKa = 7.54 PFGWPLHH8 pKa = 6.05 RR9 pKa = 11.84 RR10 pKa = 11.84 SAVPLPRR17 pKa = 11.84 WGRR20 pKa = 11.84 RR21 pKa = 11.84 GVRR24 pKa = 11.84 RR25 pKa = 11.84 GQISRR30 pKa = 11.84 SS31 pKa = 3.47
Molecular weight: 3.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3859
0
3859
1213672
10
2479
314.5
33.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.867 ± 0.063
0.735 ± 0.013
5.781 ± 0.031
5.422 ± 0.039
3.583 ± 0.024
8.856 ± 0.032
1.753 ± 0.02
4.417 ± 0.026
3.561 ± 0.031
10.034 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.018
2.395 ± 0.029
5.471 ± 0.033
3.161 ± 0.026
7.134 ± 0.043
5.106 ± 0.032
5.294 ± 0.048
7.568 ± 0.034
1.431 ± 0.016
2.144 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here