Acidianus filamentous virus 2 (isolate Italy/Pozzuoli) (AFV-2)

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Lipothrixviridae; Deltalipothrixvirus; Acidianus filamentous virus 2

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q573C9|Y093_AFV2P Uncharacterized protein ORF93 OS=Acidianus filamentous virus 2 (isolate Italy/Pozzuoli) OX=654910 GN=ORF93 PE=4 SV=1
MM1 pKa = 7.18TLSNEE6 pKa = 4.02YY7 pKa = 10.62LNAVSMMIVYY17 pKa = 9.48GQDD20 pKa = 2.97NSGTDD25 pKa = 3.58FNYY28 pKa = 10.49YY29 pKa = 9.81YY30 pKa = 11.09YY31 pKa = 11.07SATTYY36 pKa = 9.07STYY39 pKa = 11.25APTGVVAVLKK49 pKa = 10.95NSGNVVATLTGYY61 pKa = 10.31QISLSTSSVKK71 pKa = 10.62FIFYY75 pKa = 7.78DD76 pKa = 3.58TSNNEE81 pKa = 3.64YY82 pKa = 10.94SFDD85 pKa = 3.6EE86 pKa = 4.01VDD88 pKa = 4.78IYY90 pKa = 11.4TEE92 pKa = 4.13MNGTLALLVSRR103 pKa = 11.84TTGLSYY109 pKa = 11.05SKK111 pKa = 10.37SASEE115 pKa = 3.84AVVAYY120 pKa = 7.31FTLSLSQSPSLYY132 pKa = 9.72INYY135 pKa = 9.77AFMYY139 pKa = 9.84LLVPRR144 pKa = 11.84LVLQNVFPFSNYY156 pKa = 9.81VGITSYY162 pKa = 11.09SVSQVSGTISFDD174 pKa = 3.08GAGYY178 pKa = 10.89SNGEE182 pKa = 4.18LIIFLTLSTTGGANPIITAVTTQASTTTPSISSNQVLQAILPAPLPSTSSPVQYY236 pKa = 9.88PIQIGVQYY244 pKa = 10.92EE245 pKa = 4.07EE246 pKa = 4.26

Molecular weight: 26.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q573F3|Y072_AFV2P Uncharacterized protein ORF72 OS=Acidianus filamentous virus 2 (isolate Italy/Pozzuoli) OX=654910 GN=ORF72 PE=4 SV=1
MM1 pKa = 7.34SMSYY5 pKa = 10.92SLLIHH10 pKa = 6.26KK11 pKa = 7.1TRR13 pKa = 11.84KK14 pKa = 8.07WGHH17 pKa = 6.14KK18 pKa = 8.31FFKK21 pKa = 10.65FIFKK25 pKa = 10.43SYY27 pKa = 10.02IGSTMKK33 pKa = 10.91SLINKK38 pKa = 6.3VKK40 pKa = 10.35RR41 pKa = 11.84YY42 pKa = 9.98SFYY45 pKa = 11.1EE46 pKa = 3.86KK47 pKa = 10.9NLVIISNLSRR57 pKa = 11.84KK58 pKa = 8.42NVYY61 pKa = 10.05GVIFNDD67 pKa = 3.49

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

9110

46

554

175.2

19.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.412 ± 0.335

1.01 ± 0.179

5.038 ± 0.379

4.665 ± 0.385

4.479 ± 0.273

4.753 ± 0.275

1.559 ± 0.258

8.529 ± 0.349

5.895 ± 0.623

8.364 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.215

6.279 ± 0.222

4.061 ± 0.253

2.942 ± 0.333

3.117 ± 0.298

8.913 ± 0.543

7.431 ± 0.582

8.551 ± 0.363

0.944 ± 0.097

5.456 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski