Galbibacter marinus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Galbibacter

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K2PV86|K2PV86_9FLAO Glyceraldehyde-3-phosphate dehydrogenase OS=Galbibacter marinus OX=555500 GN=I215_08031 PE=3 SV=1
MM1 pKa = 7.55GGLLISCDD9 pKa = 3.4DD10 pKa = 4.39HH11 pKa = 6.59EE12 pKa = 5.56NSMGEE17 pKa = 4.01KK18 pKa = 10.06LDD20 pKa = 3.82PSEE23 pKa = 4.53LKK25 pKa = 10.85FEE27 pKa = 4.34IEE29 pKa = 3.57QDD31 pKa = 3.47LTIDD35 pKa = 3.28QGGNTVILTNNTPGTIPIWNYY56 pKa = 8.5GTGRR60 pKa = 11.84SNKK63 pKa = 10.22AIDD66 pKa = 3.77TIRR69 pKa = 11.84FAFAGEE75 pKa = 3.88YY76 pKa = 9.6TIEE79 pKa = 4.13FSVMTEE85 pKa = 3.95GGLVEE90 pKa = 5.05ADD92 pKa = 3.41PVTISVTDD100 pKa = 3.41NNFEE104 pKa = 4.24YY105 pKa = 11.13VEE107 pKa = 4.12NEE109 pKa = 3.42LWLKK113 pKa = 10.96LSGGVGNAKK122 pKa = 8.51TWVLDD127 pKa = 3.73LDD129 pKa = 3.99AEE131 pKa = 4.75GVSKK135 pKa = 10.91YY136 pKa = 9.84FVGPQYY142 pKa = 10.76FYY144 pKa = 10.73GTNNGWLEE152 pKa = 4.72GGDD155 pKa = 3.56EE156 pKa = 4.12WSGGSTGCYY165 pKa = 9.99GADD168 pKa = 2.95CWNWNPDD175 pKa = 2.85WPGNSWIMAAGDD187 pKa = 3.93YY188 pKa = 10.18GTMTFSLDD196 pKa = 2.88GGPFVEE202 pKa = 4.92VDD204 pKa = 3.22NLMFAQQGKK213 pKa = 9.63QSGTYY218 pKa = 9.3YY219 pKa = 11.03LDD221 pKa = 4.37ANSHH225 pKa = 5.33TLTMTDD231 pKa = 3.12AQLLHH236 pKa = 6.36NAEE239 pKa = 4.42NDD241 pKa = 3.64DD242 pKa = 4.87CVSNWSDD249 pKa = 3.07MKK251 pKa = 10.79IFSLTEE257 pKa = 3.49DD258 pKa = 3.49TMQLGVLRR266 pKa = 11.84KK267 pKa = 9.7SSCDD271 pKa = 3.01GAAYY275 pKa = 10.33LVFNFISKK283 pKa = 9.91EE284 pKa = 3.95YY285 pKa = 9.64SDD287 pKa = 3.34NWVPEE292 pKa = 4.16EE293 pKa = 4.23TEE295 pKa = 3.87PTIDD299 pKa = 3.71EE300 pKa = 4.68GFDD303 pKa = 3.43PSLEE307 pKa = 4.14AGQLLEE313 pKa = 4.62MLTGGQGSGRR323 pKa = 11.84VWAMDD328 pKa = 3.59AAGNPVDD335 pKa = 4.21WVTAGAGWTVDD346 pKa = 3.43ASSSADD352 pKa = 3.34WGWNSSWSTVASDD365 pKa = 2.58SWIRR369 pKa = 11.84FDD371 pKa = 4.27QWNGMQTYY379 pKa = 8.35TRR381 pKa = 11.84NQSGVEE387 pKa = 3.88TSGTFTINEE396 pKa = 4.09ATNEE400 pKa = 3.88ITLSGEE406 pKa = 4.17VLIQNPGNWMTPTTNTITVIKK427 pKa = 10.62AFDD430 pKa = 4.1DD431 pKa = 4.17YY432 pKa = 9.77QTKK435 pKa = 10.68GIWFGTSYY443 pKa = 11.25DD444 pKa = 3.84SEE446 pKa = 4.63KK447 pKa = 11.28DD448 pKa = 2.84EE449 pKa = 4.38WLAFHH454 pKa = 6.7YY455 pKa = 10.07IIPP458 pKa = 4.18

Molecular weight: 50.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K2P0B3|K2P0B3_9FLAO Elongation factor G OS=Galbibacter marinus OX=555500 GN=fusA PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVSGFDD22 pKa = 4.27AITTDD27 pKa = 3.56KK28 pKa = 11.07PEE30 pKa = 4.36KK31 pKa = 10.55SLLAPIKK38 pKa = 9.77KK39 pKa = 8.55TGGRR43 pKa = 11.84NSQGKK48 pKa = 6.49MTMRR52 pKa = 11.84YY53 pKa = 9.67LGGGHH58 pKa = 5.72KK59 pKa = 9.83RR60 pKa = 11.84KK61 pKa = 9.98YY62 pKa = 10.35RR63 pKa = 11.84IIDD66 pKa = 3.68FKK68 pKa = 10.73RR69 pKa = 11.84AKK71 pKa = 9.36TGVEE75 pKa = 3.86ATVEE79 pKa = 4.31SIQYY83 pKa = 10.1DD84 pKa = 3.73PNRR87 pKa = 11.84TAFIALIVYY96 pKa = 9.1TDD98 pKa = 3.79GEE100 pKa = 4.25KK101 pKa = 10.59SYY103 pKa = 10.83IIAPNGLQVGQKK115 pKa = 9.39VRR117 pKa = 11.84SGAEE121 pKa = 3.63ATPEE125 pKa = 3.86IGNAMPLSQVPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVMARR156 pKa = 11.84SAGAFAQLMARR167 pKa = 11.84EE168 pKa = 4.45GKK170 pKa = 10.09YY171 pKa = 9.05ATIKK175 pKa = 10.52LPSGEE180 pKa = 4.07TRR182 pKa = 11.84YY183 pKa = 10.7VLADD187 pKa = 3.65CFATIGAVSNSDD199 pKa = 3.2HH200 pKa = 6.21QLLVSGKK207 pKa = 9.87AGRR210 pKa = 11.84TRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVVMNPVDD229 pKa = 3.47HH230 pKa = 7.05PMGGGEE236 pKa = 3.89GRR238 pKa = 11.84ASGGHH243 pKa = 5.11PRR245 pKa = 11.84SRR247 pKa = 11.84NGVPAKK253 pKa = 10.34GYY255 pKa = 7.34RR256 pKa = 11.84TRR258 pKa = 11.84AKK260 pKa = 10.32AKK262 pKa = 10.15ASNKK266 pKa = 10.05YY267 pKa = 9.32IVEE270 pKa = 4.08RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3099

0

3099

1050357

35

4820

338.9

38.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.356 ± 0.043

0.73 ± 0.013

5.709 ± 0.035

6.505 ± 0.038

4.892 ± 0.031

6.586 ± 0.044

1.942 ± 0.023

7.676 ± 0.044

7.249 ± 0.052

9.403 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.022

5.687 ± 0.039

3.536 ± 0.025

3.976 ± 0.029

3.689 ± 0.023

6.668 ± 0.033

5.574 ± 0.049

6.283 ± 0.034

1.112 ± 0.016

4.157 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski