Amino acid dipepetide frequency for Clostridium sp. CAG:217

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.259AlaAla: 11.259 ± 0.224	1.625AlaCys: 1.625 ± 0.056	6.284AlaAsp: 6.284 ± 0.108	5.337AlaGlu: 5.337 ± 0.099	3.953AlaPhe: 3.953 ± 0.094	7.339AlaGly: 7.339 ± 0.13	1.652AlaHis: 1.652 ± 0.054	4.563AlaIle: 4.563 ± 0.099	5.818AlaLys: 5.818 ± 0.126	9.963AlaLeu: 9.963 ± 0.165	2.684AlaMet: 2.684 ± 0.069	2.868AlaAsn: 2.868 ± 0.089	3.36AlaPro: 3.36 ± 0.08	3.558AlaGln: 3.558 ± 0.087	4.154AlaArg: 4.154 ± 0.106	3.531AlaSer: 3.531 ± 0.088	4.635AlaThr: 4.635 ± 0.12	8.404AlaVal: 8.404 ± 0.137	0.595AlaTrp: 0.595 ± 0.032	3.609AlaTyr: 3.609 ± 0.087	0.013AlaXaa: 0.013 ± 0.005
Cys	1.771CysAla: 1.771 ± 0.067	0.352CysCys: 0.352 ± 0.032	1.111CysAsp: 1.111 ± 0.045	0.721CysGlu: 0.721 ± 0.042	0.709CysPhe: 0.709 ± 0.037	1.784CysGly: 1.784 ± 0.064	0.386CysHis: 0.386 ± 0.027	0.914CysIle: 0.914 ± 0.037	0.992CysLys: 0.992 ± 0.041	1.454CysLeu: 1.454 ± 0.054	0.399CysMet: 0.399 ± 0.032	0.658CysAsn: 0.658 ± 0.038	0.916CysPro: 0.916 ± 0.045	0.546CysGln: 0.546 ± 0.033	0.956CysArg: 0.956 ± 0.047	0.981CysSer: 0.981 ± 0.039	1.196CysThr: 1.196 ± 0.06	1.18CysVal: 1.18 ± 0.053	0.144CysTrp: 0.144 ± 0.017	0.613CysTyr: 0.613 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	5.117AspAla: 5.117 ± 0.103	1.158AspCys: 1.158 ± 0.051	3.192AspAsp: 3.192 ± 0.096	3.524AspGlu: 3.524 ± 0.102	2.981AspPhe: 2.981 ± 0.089	5.261AspGly: 5.261 ± 0.122	1.088AspHis: 1.088 ± 0.044	3.373AspIle: 3.373 ± 0.08	3.564AspLys: 3.564 ± 0.083	5.573AspLeu: 5.573 ± 0.113	1.694AspMet: 1.694 ± 0.059	2.045AspAsn: 2.045 ± 0.079	2.494AspPro: 2.494 ± 0.073	1.684AspGln: 1.684 ± 0.061	3.169AspArg: 3.169 ± 0.087	2.909AspSer: 2.909 ± 0.085	3.656AspThr: 3.656 ± 0.088	4.087AspVal: 4.087 ± 0.089	0.662AspTrp: 0.662 ± 0.035	2.972AspTyr: 2.972 ± 0.087	0.002AspXaa: 0.002 ± 0.001
Glu	4.624GluAla: 4.624 ± 0.103	0.68GluCys: 0.68 ± 0.034	3.236GluAsp: 3.236 ± 0.099	4.152GluGlu: 4.152 ± 0.115	1.791GluPhe: 1.791 ± 0.057	3.241GluGly: 3.241 ± 0.071	1.1GluHis: 1.1 ± 0.044	3.982GluIle: 3.982 ± 0.105	4.779GluLys: 4.779 ± 0.105	5.165GluLeu: 5.165 ± 0.115	1.968GluMet: 1.968 ± 0.06	3.351GluAsn: 3.351 ± 0.08	1.822GluPro: 1.822 ± 0.063	2.691GluGln: 2.691 ± 0.084	2.945GluArg: 2.945 ± 0.083	2.72GluSer: 2.72 ± 0.062	3.073GluThr: 3.073 ± 0.075	3.349GluVal: 3.349 ± 0.092	0.39GluTrp: 0.39 ± 0.029	2.047GluTyr: 2.047 ± 0.073	0.0GluXaa: 0.0 ± 0.0
Phe	3.887PheAla: 3.887 ± 0.085	0.828PheCys: 0.828 ± 0.038	2.933PheAsp: 2.933 ± 0.081	2.027PheGlu: 2.027 ± 0.07	1.739PhePhe: 1.739 ± 0.069	3.288PheGly: 3.288 ± 0.085	0.757PheHis: 0.757 ± 0.04	2.254PheIle: 2.254 ± 0.072	1.926PheLys: 1.926 ± 0.065	3.472PheLeu: 3.472 ± 0.096	0.943PheMet: 0.943 ± 0.043	1.54PheAsn: 1.54 ± 0.057	1.253PhePro: 1.253 ± 0.046	1.124PheGln: 1.124 ± 0.043	1.593PheArg: 1.593 ± 0.05	2.64PheSer: 2.64 ± 0.079	2.602PheThr: 2.602 ± 0.066	2.896PheVal: 2.896 ± 0.064	0.361PheTrp: 0.361 ± 0.026	1.666PheTyr: 1.666 ± 0.063	0.004PheXaa: 0.004 ± 0.003
Gly	6.954GlyAla: 6.954 ± 0.133	1.556GlyCys: 1.556 ± 0.058	4.392GlyAsp: 4.392 ± 0.094	4.074GlyGlu: 4.074 ± 0.096	3.019GlyPhe: 3.019 ± 0.077	5.512GlyGly: 5.512 ± 0.109	1.445GlyHis: 1.445 ± 0.058	4.774GlyIle: 4.774 ± 0.11	5.414GlyLys: 5.414 ± 0.096	6.141GlyLeu: 6.141 ± 0.115	2.217GlyMet: 2.217 ± 0.076	3.05GlyAsn: 3.05 ± 0.086	1.475GlyPro: 1.475 ± 0.051	2.46GlyGln: 2.46 ± 0.074	3.367GlyArg: 3.367 ± 0.088	4.222GlySer: 4.222 ± 0.091	4.835GlyThr: 4.835 ± 0.109	6.28GlyVal: 6.28 ± 0.124	0.739GlyTrp: 0.739 ± 0.042	3.299GlyTyr: 3.299 ± 0.084	0.007GlyXaa: 0.007 ± 0.003
His	1.493HisAla: 1.493 ± 0.056	0.456HisCys: 0.456 ± 0.031	0.884HisAsp: 0.884 ± 0.041	0.718HisGlu: 0.718 ± 0.038	0.934HisPhe: 0.934 ± 0.046	1.464HisGly: 1.464 ± 0.054	0.467HisHis: 0.467 ± 0.035	1.455HisIle: 1.455 ± 0.061	1.006HisLys: 1.006 ± 0.044	1.795HisLeu: 1.795 ± 0.058	0.411HisMet: 0.411 ± 0.029	0.783HisAsn: 0.783 ± 0.034	1.181HisPro: 1.181 ± 0.049	0.651HisGln: 0.651 ± 0.035	1.216HisArg: 1.216 ± 0.056	1.037HisSer: 1.037 ± 0.052	1.291HisThr: 1.291 ± 0.049	0.981HisVal: 0.981 ± 0.04	0.207HisTrp: 0.207 ± 0.02	0.794HisTyr: 0.794 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	5.638IleAla: 5.638 ± 0.116	1.196IleCys: 1.196 ± 0.045	3.751IleAsp: 3.751 ± 0.092	3.351IleGlu: 3.351 ± 0.082	2.101IlePhe: 2.101 ± 0.061	4.386IleGly: 4.386 ± 0.107	1.18IleHis: 1.18 ± 0.051	3.127IleIle: 3.127 ± 0.086	3.111IleLys: 3.111 ± 0.085	5.319IleLeu: 5.319 ± 0.125	1.266IleMet: 1.266 ± 0.056	2.272IleAsn: 2.272 ± 0.083	2.492IlePro: 2.492 ± 0.082	1.71IleGln: 1.71 ± 0.056	2.794IleArg: 2.794 ± 0.081	3.539IleSer: 3.539 ± 0.09	3.905IleThr: 3.905 ± 0.093	4.056IleVal: 4.056 ± 0.102	0.476IleTrp: 0.476 ± 0.033	2.087IleTyr: 2.087 ± 0.069	0.0IleXaa: 0.0 ± 0.0
Lys	5.748LysAla: 5.748 ± 0.116	0.822LysCys: 0.822 ± 0.044	3.49LysAsp: 3.49 ± 0.089	4.123LysGlu: 4.123 ± 0.106	1.692LysPhe: 1.692 ± 0.058	3.827LysGly: 3.827 ± 0.081	0.918LysHis: 0.918 ± 0.04	3.858LysIle: 3.858 ± 0.094	5.109LysLys: 5.109 ± 0.121	4.677LysLeu: 4.677 ± 0.097	1.939LysMet: 1.939 ± 0.066	3.012LysAsn: 3.012 ± 0.092	2.121LysPro: 2.121 ± 0.062	2.06LysGln: 2.06 ± 0.068	2.671LysArg: 2.671 ± 0.075	3.234LysSer: 3.234 ± 0.084	3.843LysThr: 3.843 ± 0.1	3.991LysVal: 3.991 ± 0.08	0.537LysTrp: 0.537 ± 0.032	2.355LysTyr: 2.355 ± 0.072	0.004LysXaa: 0.004 ± 0.003
Leu	8.354LeuAla: 8.354 ± 0.151	1.973LeuCys: 1.973 ± 0.066	5.34LeuAsp: 5.34 ± 0.117	5.483LeuGlu: 5.483 ± 0.1	3.957LeuPhe: 3.957 ± 0.107	6.404LeuGly: 6.404 ± 0.134	2.002LeuHis: 2.002 ± 0.067	4.87LeuIle: 4.87 ± 0.107	4.267LeuLys: 4.267 ± 0.096	9.319LeuLeu: 9.319 ± 0.197	2.22LeuMet: 2.22 ± 0.071	3.214LeuAsn: 3.214 ± 0.083	4.325LeuPro: 4.325 ± 0.1	3.993LeuGln: 3.993 ± 0.095	4.723LeuArg: 4.723 ± 0.105	5.64LeuSer: 5.64 ± 0.118	5.688LeuThr: 5.688 ± 0.103	6.087LeuVal: 6.087 ± 0.108	0.777LeuTrp: 0.777 ± 0.039	3.308LeuTyr: 3.308 ± 0.083	0.004LeuXaa: 0.004 ± 0.003
Met	2.491MetAla: 2.491 ± 0.059	0.337MetCys: 0.337 ± 0.025	1.8MetAsp: 1.8 ± 0.064	1.735MetGlu: 1.735 ± 0.065	0.824MetPhe: 0.824 ± 0.037	1.959MetGly: 1.959 ± 0.063	0.532MetHis: 0.532 ± 0.032	1.533MetIle: 1.533 ± 0.05	1.776MetLys: 1.776 ± 0.054	2.411MetLeu: 2.411 ± 0.081	0.721MetMet: 0.721 ± 0.039	1.226MetAsn: 1.226 ± 0.04	1.037MetPro: 1.037 ± 0.041	1.154MetGln: 1.154 ± 0.039	1.38MetArg: 1.38 ± 0.053	1.365MetSer: 1.365 ± 0.056	1.76MetThr: 1.76 ± 0.06	1.686MetVal: 1.686 ± 0.055	0.193MetTrp: 0.193 ± 0.021	0.777MetTyr: 0.777 ± 0.041	0.0MetXaa: 0.0 ± 0.0
Asn	3.692AsnAla: 3.692 ± 0.104	0.729AsnCys: 0.729 ± 0.044	2.125AsnAsp: 2.125 ± 0.072	1.87AsnGlu: 1.87 ± 0.061	1.414AsnPhe: 1.414 ± 0.054	3.636AsnGly: 3.636 ± 0.1	0.774AsnHis: 0.774 ± 0.041	2.568AsnIle: 2.568 ± 0.078	2.244AsnLys: 2.244 ± 0.076	3.122AsnLeu: 3.122 ± 0.076	1.125AsnMet: 1.125 ± 0.046	1.504AsnAsn: 1.504 ± 0.058	1.93AsnPro: 1.93 ± 0.064	1.291AsnGln: 1.291 ± 0.049	2.011AsnArg: 2.011 ± 0.063	1.977AsnSer: 1.977 ± 0.079	2.482AsnThr: 2.482 ± 0.072	2.631AsnVal: 2.631 ± 0.072	0.388AsnTrp: 0.388 ± 0.027	1.576AsnTyr: 1.576 ± 0.06	0.004AsnXaa: 0.004 ± 0.002
Pro	3.919ProAla: 3.919 ± 0.087	0.655ProCys: 0.655 ± 0.036	2.473ProAsp: 2.473 ± 0.07	2.974ProGlu: 2.974 ± 0.072	1.684ProPhe: 1.684 ± 0.059	3.005ProGly: 3.005 ± 0.077	0.675ProHis: 0.675 ± 0.034	1.964ProIle: 1.964 ± 0.064	2.121ProLys: 2.121 ± 0.065	3.232ProLeu: 3.232 ± 0.084	0.972ProMet: 0.972 ± 0.042	1.401ProAsn: 1.401 ± 0.059	1.207ProPro: 1.207 ± 0.054	1.356ProGln: 1.356 ± 0.052	1.501ProArg: 1.501 ± 0.061	1.712ProSer: 1.712 ± 0.05	2.327ProThr: 2.327 ± 0.076	3.318ProVal: 3.318 ± 0.074	0.391ProTrp: 0.391 ± 0.027	1.546ProTyr: 1.546 ± 0.056	0.005ProXaa: 0.005 ± 0.003
Gln	3.497GlnAla: 3.497 ± 0.092	0.438GlnCys: 0.438 ± 0.032	1.688GlnAsp: 1.688 ± 0.057	1.926GlnGlu: 1.926 ± 0.051	1.21GlnPhe: 1.21 ± 0.044	2.278GlnGly: 2.278 ± 0.061	0.608GlnHis: 0.608 ± 0.04	2.323GlnIle: 2.323 ± 0.066	2.429GlnLys: 2.429 ± 0.09	3.405GlnLeu: 3.405 ± 0.083	1.246GlnMet: 1.246 ± 0.049	1.712GlnAsn: 1.712 ± 0.056	1.344GlnPro: 1.344 ± 0.052	1.594GlnGln: 1.594 ± 0.075	1.959GlnArg: 1.959 ± 0.069	1.829GlnSer: 1.829 ± 0.059	2.135GlnThr: 2.135 ± 0.069	3.008GlnVal: 3.008 ± 0.08	0.332GlnTrp: 0.332 ± 0.029	1.483GlnTyr: 1.483 ± 0.056	0.004GlnXaa: 0.004 ± 0.003
Arg	4.006ArgAla: 4.006 ± 0.087	0.833ArgCys: 0.833 ± 0.041	2.548ArgAsp: 2.548 ± 0.073	3.05ArgGlu: 3.05 ± 0.084	2.052ArgPhe: 2.052 ± 0.061	2.981ArgGly: 2.981 ± 0.082	1.044ArgHis: 1.044 ± 0.049	2.936ArgIle: 2.936 ± 0.084	2.579ArgLys: 2.579 ± 0.064	5.052ArgLeu: 5.052 ± 0.108	1.398ArgMet: 1.398 ± 0.051	1.593ArgAsn: 1.593 ± 0.051	1.757ArgPro: 1.757 ± 0.064	2.319ArgGln: 2.319 ± 0.075	3.302ArgArg: 3.302 ± 0.101	2.478ArgSer: 2.478 ± 0.071	2.785ArgThr: 2.785 ± 0.074	3.45ArgVal: 3.45 ± 0.09	0.474ArgTrp: 0.474 ± 0.033	2.079ArgTyr: 2.079 ± 0.06	0.002ArgXaa: 0.002 ± 0.002
Ser	5.407SerAla: 5.407 ± 0.124	0.783SerCys: 0.783 ± 0.036	3.044SerAsp: 3.044 ± 0.073	2.309SerGlu: 2.309 ± 0.074	2.155SerPhe: 2.155 ± 0.064	4.967SerGly: 4.967 ± 0.119	0.978SerHis: 0.978 ± 0.039	2.864SerIle: 2.864 ± 0.085	3.113SerLys: 3.113 ± 0.089	4.379SerLeu: 4.379 ± 0.109	1.344SerMet: 1.344 ± 0.054	1.832SerAsn: 1.832 ± 0.066	1.926SerPro: 1.926 ± 0.065	1.65SerGln: 1.65 ± 0.059	2.574SerArg: 2.574 ± 0.074	2.725SerSer: 2.725 ± 0.081	3.08SerThr: 3.08 ± 0.1	4.291SerVal: 4.291 ± 0.094	0.473SerTrp: 0.473 ± 0.031	2.033SerTyr: 2.033 ± 0.066	0.005SerXaa: 0.005 ± 0.003
Thr	6.722ThrAla: 6.722 ± 0.146	0.896ThrCys: 0.896 ± 0.041	4.343ThrAsp: 4.343 ± 0.093	3.297ThrGlu: 3.297 ± 0.096	2.368ThrPhe: 2.368 ± 0.063	5.228ThrGly: 5.228 ± 0.105	1.167ThrHis: 1.167 ± 0.04	3.113ThrIle: 3.113 ± 0.075	3.075ThrLys: 3.075 ± 0.078	5.461ThrLeu: 5.461 ± 0.104	1.234ThrMet: 1.234 ± 0.051	1.984ThrAsn: 1.984 ± 0.075	2.804ThrPro: 2.804 ± 0.071	1.784ThrGln: 1.784 ± 0.053	2.373ThrArg: 2.373 ± 0.067	2.485ThrSer: 2.485 ± 0.079	3.562ThrThr: 3.562 ± 0.139	6.476ThrVal: 6.476 ± 0.143	0.444ThrTrp: 0.444 ± 0.034	2.372ThrTyr: 2.372 ± 0.075	0.004ThrXaa: 0.004 ± 0.003
Val	6.476ValAla: 6.476 ± 0.123	1.544ValCys: 1.544 ± 0.053	4.247ValAsp: 4.247 ± 0.1	4.179ValGlu: 4.179 ± 0.097	3.021ValPhe: 3.021 ± 0.075	4.868ValGly: 4.868 ± 0.096	1.446ValHis: 1.446 ± 0.054	4.539ValIle: 4.539 ± 0.108	3.777ValLys: 3.777 ± 0.093	7.946ValLeu: 7.946 ± 0.14	1.789ValMet: 1.789 ± 0.058	2.633ValAsn: 2.633 ± 0.071	3.356ValPro: 3.356 ± 0.085	3.115ValGln: 3.115 ± 0.07	3.631ValArg: 3.631 ± 0.089	4.258ValSer: 4.258 ± 0.101	5.028ValThr: 5.028 ± 0.116	5.472ValVal: 5.472 ± 0.135	0.651ValTrp: 0.651 ± 0.035	2.743ValTyr: 2.743 ± 0.083	0.005ValXaa: 0.005 ± 0.003
Trp	0.656TrpAla: 0.656 ± 0.035	0.191TrpCys: 0.191 ± 0.02	0.539TrpAsp: 0.539 ± 0.033	0.413TrpGlu: 0.413 ± 0.027	0.386TrpPhe: 0.386 ± 0.027	0.666TrpGly: 0.666 ± 0.044	0.229TrpHis: 0.229 ± 0.022	0.471TrpIle: 0.471 ± 0.032	0.456TrpLys: 0.456 ± 0.031	0.837TrpLeu: 0.837 ± 0.038	0.216TrpMet: 0.216 ± 0.018	0.483TrpAsn: 0.483 ± 0.035	0.17TrpPro: 0.17 ± 0.019	0.462TrpGln: 0.462 ± 0.027	0.4TrpArg: 0.4 ± 0.029	0.541TrpSer: 0.541 ± 0.036	0.465TrpThr: 0.465 ± 0.034	0.579TrpVal: 0.579 ± 0.037	0.133TrpTrp: 0.133 ± 0.015	0.427TrpTyr: 0.427 ± 0.025	0.002TrpXaa: 0.002 ± 0.002
Tyr	3.475TyrAla: 3.475 ± 0.093	0.729TyrCys: 0.729 ± 0.039	2.785TyrAsp: 2.785 ± 0.083	1.841TyrGlu: 1.841 ± 0.058	1.742TyrPhe: 1.742 ± 0.057	3.135TyrGly: 3.135 ± 0.079	0.779TyrHis: 0.779 ± 0.037	2.274TyrIle: 2.274 ± 0.074	2.473TyrLys: 2.473 ± 0.079	3.223TyrLeu: 3.223 ± 0.082	0.913TyrMet: 0.913 ± 0.039	1.928TyrAsn: 1.928 ± 0.065	1.454TyrPro: 1.454 ± 0.053	1.243TyrGln: 1.243 ± 0.045	1.991TyrArg: 1.991 ± 0.059	2.108TyrSer: 2.108 ± 0.069	2.839TyrThr: 2.839 ± 0.099	2.5TyrVal: 2.5 ± 0.066	0.355TyrTrp: 0.355 ± 0.029	1.879TyrTyr: 1.879 ± 0.074	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.016XaaAla: 0.016 ± 0.006	0.002XaaCys: 0.002 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.005XaaGly: 0.005 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.004XaaLeu: 0.004 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.002	0.013XaaPro: 0.013 ± 0.005	0.002XaaGln: 0.002 ± 0.002	0.013XaaArg: 0.013 ± 0.006	0.0XaaSer: 0.0 ± 0.0	0.004XaaThr: 0.004 ± 0.002	0.002XaaVal: 0.002 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.085XaaXaa: 0.085 ± 0.019

Statistics based on 1746 proteins (554469 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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