Rhizobium oryzae
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4627 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q9AUC5|A0A1Q9AUC5_9RHIZ Protein SlyX homolog OS=Rhizobium oryzae OX=464029 GN=slyX PE=3 SV=1
MM1 pKa = 6.7 STEE4 pKa = 4.24 TKK6 pKa = 9.57 VLPMISGTSANDD18 pKa = 3.68 YY19 pKa = 10.23 IASTGSGATLVGGAGNDD36 pKa = 3.59 LYY38 pKa = 11.07 FVTSADD44 pKa = 3.47 DD45 pKa = 4.19 VIIEE49 pKa = 4.43 KK50 pKa = 10.06 PGEE53 pKa = 4.56 GIDD56 pKa = 4.06 SVITYY61 pKa = 7.49 RR62 pKa = 11.84 TSYY65 pKa = 9.9 TLGDD69 pKa = 3.5 NVEE72 pKa = 4.21 NLSVLGNDD80 pKa = 4.11 LYY82 pKa = 11.64 GVGNALDD89 pKa = 3.75 NVLVGGSGRR98 pKa = 11.84 QTLVGGAGNDD108 pKa = 3.7 TLTGGAGSDD117 pKa = 3.51 IFVLTQGGGSDD128 pKa = 4.0 TITDD132 pKa = 4.38 FDD134 pKa = 4.8 ASDD137 pKa = 3.28 IVQLGVYY144 pKa = 9.18 GFKK147 pKa = 10.93 NFAEE151 pKa = 4.48 VSDD154 pKa = 5.48 SLVQDD159 pKa = 3.56 NANVVLKK166 pKa = 11.06 LNDD169 pKa = 3.53 GSQLTFLNKK178 pKa = 9.77 TIADD182 pKa = 3.97 FQSSNFQYY190 pKa = 11.55 ALDD193 pKa = 4.03 TSHH196 pKa = 7.34 LKK198 pKa = 8.15 QTFADD203 pKa = 3.91 EE204 pKa = 4.8 FNTLSLQQEE213 pKa = 4.28 GGMWRR218 pKa = 11.84 TWHH221 pKa = 6.55 NNGLSDD227 pKa = 5.24 GGQAYY232 pKa = 10.42 LNPTNSTGVNPFSVTDD248 pKa = 4.08 GVLTIHH254 pKa = 7.49 ADD256 pKa = 3.66 RR257 pKa = 11.84 ASAEE261 pKa = 4.19 TAALTGKK268 pKa = 9.73 EE269 pKa = 4.05 FTSGIMSTRR278 pKa = 11.84 DD279 pKa = 3.31 TFSQTYY285 pKa = 9.57 GYY287 pKa = 10.79 FEE289 pKa = 4.27 IRR291 pKa = 11.84 ADD293 pKa = 3.74 LPSQKK298 pKa = 10.12 GVCPAFWLLPANGSWPPEE316 pKa = 3.67 LDD318 pKa = 3.2 VFEE321 pKa = 4.69 QVGNDD326 pKa = 3.45 PSKK329 pKa = 11.33 VYY331 pKa = 9.19 LTAHH335 pKa = 6.16 SNSTGTPTATGISSWIGDD353 pKa = 3.91 TGEE356 pKa = 4.18 GMHH359 pKa = 6.67 TYY361 pKa = 10.87 GLLWTKK367 pKa = 10.89 DD368 pKa = 3.38 VLVWYY373 pKa = 10.17 LDD375 pKa = 3.26 GVEE378 pKa = 4.72 AYY380 pKa = 10.35 RR381 pKa = 11.84 IATPADD387 pKa = 3.33 MHH389 pKa = 5.61 QAMYY393 pKa = 10.7 LVTNMSVGGGFAGSTDD409 pKa = 3.33 ASFTGADD416 pKa = 3.54 FKK418 pKa = 10.92 IDD420 pKa = 3.92 YY421 pKa = 9.74 IHH423 pKa = 7.88 AYY425 pKa = 8.44 QLEE428 pKa = 4.61 EE429 pKa = 3.96 NLGTRR434 pKa = 11.84 QSITGEE440 pKa = 3.79 KK441 pKa = 9.72 WSITLDD447 pKa = 3.54 NNGDD451 pKa = 3.93 DD452 pKa = 3.81 VTLTGTLNINATGNAKK468 pKa = 10.63 DD469 pKa = 4.31 NILIGNSGNNTLDD482 pKa = 3.54 GKK484 pKa = 11.01 VGADD488 pKa = 3.46 TMIGGQGDD496 pKa = 3.55 DD497 pKa = 3.72 TYY499 pKa = 11.34 IVDD502 pKa = 3.53 NAGDD506 pKa = 3.83 VVVEE510 pKa = 4.15 NANEE514 pKa = 4.1 GTDD517 pKa = 3.46 TVRR520 pKa = 11.84 ASISYY525 pKa = 9.59 VLGANVEE532 pKa = 4.32 KK533 pKa = 11.02 LFLTGTDD540 pKa = 3.48 NLHH543 pKa = 5.44 GTGNEE548 pKa = 4.15 LDD550 pKa = 3.4 NTIYY554 pKa = 10.96 GNSGDD559 pKa = 3.96 NVINGAGGADD569 pKa = 3.72 VMIGGAGNDD578 pKa = 2.99 IYY580 pKa = 11.65 YY581 pKa = 10.36 VDD583 pKa = 3.98 NPGDD587 pKa = 4.09 IVTEE591 pKa = 4.41 WSNGGIDD598 pKa = 3.62 TVYY601 pKa = 11.21 SSISYY606 pKa = 10.08 ALPNQVEE613 pKa = 4.31 NLTLTGSDD621 pKa = 4.14 NIDD624 pKa = 3.26 ATGNWMDD631 pKa = 5.24 NILIGNDD638 pKa = 3.13 GNNVLRR644 pKa = 11.84 GMGGTDD650 pKa = 3.19 TLIGGKK656 pKa = 10.23 GDD658 pKa = 3.21 DD659 pKa = 3.73 TYY661 pKa = 11.93 YY662 pKa = 11.03 VDD664 pKa = 5.13 SDD666 pKa = 3.54 DD667 pKa = 4.83 TIIEE671 pKa = 4.26 KK672 pKa = 10.64 ADD674 pKa = 3.35 GGIDD678 pKa = 3.35 TVYY681 pKa = 11.04 ASSSYY686 pKa = 9.6 TLSANVEE693 pKa = 4.28 TLILTGNWTSNGTGNNLDD711 pKa = 3.76 NLLIGNSCANTLDD724 pKa = 3.71 GGRR727 pKa = 11.84 GADD730 pKa = 3.58 TMIGGAGNDD739 pKa = 3.55 TYY741 pKa = 11.24 FVDD744 pKa = 3.76 NPGDD748 pKa = 3.65 IVIEE752 pKa = 4.1 NAGEE756 pKa = 4.23 GVDD759 pKa = 3.65 RR760 pKa = 11.84 VYY762 pKa = 11.57 SSISYY767 pKa = 8.6 TLTANVEE774 pKa = 4.16 MLLLTGKK781 pKa = 10.58 ADD783 pKa = 4.28 LNATGNSLSNTIYY796 pKa = 11.13 GNDD799 pKa = 3.63 GNNIIDD805 pKa = 4.33 GGTGADD811 pKa = 3.34 TMSGQGGNDD820 pKa = 3.06 TYY822 pKa = 11.61 YY823 pKa = 11.16 VDD825 pKa = 4.55 NIYY828 pKa = 11.17 DD829 pKa = 5.15 KK830 pKa = 10.83 IIEE833 pKa = 4.14 WSNGGIDD840 pKa = 3.65 TVLSSVSYY848 pKa = 10.77 SLADD852 pKa = 3.37 NVEE855 pKa = 3.82 NLTLIGLANLNATGNWQDD873 pKa = 4.11 NILIGNAGNNILDD886 pKa = 3.89 GSKK889 pKa = 10.84 GADD892 pKa = 3.36 LMKK895 pKa = 10.82 GGAGDD900 pKa = 3.36 DD901 pKa = 3.8 TYY903 pKa = 11.24 IVEE906 pKa = 4.32 NVGDD910 pKa = 3.91 VVVEE914 pKa = 4.1 NPGEE918 pKa = 4.44 GIDD921 pKa = 4.12 TVKK924 pKa = 10.64 SWISYY929 pKa = 9.81 RR930 pKa = 11.84 LTDD933 pKa = 3.74 NVEE936 pKa = 3.96 KK937 pKa = 11.06 LMLQSAGNINGYY949 pKa = 10.88 GNDD952 pKa = 4.0 LDD954 pKa = 3.95 NTIVGSIGNNIIDD967 pKa = 4.26 GGAGADD973 pKa = 3.5 RR974 pKa = 11.84 MQGGLGNDD982 pKa = 3.44 TYY984 pKa = 11.78 YY985 pKa = 11.23 VDD987 pKa = 3.82 NPGDD991 pKa = 4.6 LVIEE995 pKa = 4.47 WSGEE999 pKa = 4.13 GNDD1002 pKa = 3.9 TVYY1005 pKa = 11.34 SSISYY1010 pKa = 8.48 TLVNNVEE1017 pKa = 3.96 NLILTGTANLDD1028 pKa = 3.48 GCGNGLDD1035 pKa = 3.68 NTLIGNDD1042 pKa = 3.39 GNNRR1046 pKa = 11.84 LDD1048 pKa = 3.71 GGAGNDD1054 pKa = 3.35 ILQGGLGVDD1063 pKa = 3.83 ILNGGAGNDD1072 pKa = 3.3 TFVFKK1077 pKa = 9.84 TAADD1081 pKa = 4.03 SAVGTPDD1088 pKa = 4.62 TINDD1092 pKa = 3.73 FQKK1095 pKa = 11.34 GDD1097 pKa = 3.86 IIDD1100 pKa = 4.16 LRR1102 pKa = 11.84 DD1103 pKa = 3.54 MYY1105 pKa = 11.06 AGTMTFLGTGDD1116 pKa = 3.68 FTGHH1120 pKa = 5.97 SGEE1123 pKa = 4.35 VQISAYY1129 pKa = 10.55 GKK1131 pKa = 8.77 GTMINIDD1138 pKa = 3.81 LDD1140 pKa = 3.9 GDD1142 pKa = 3.72 KK1143 pKa = 10.99 HH1144 pKa = 7.55 IDD1146 pKa = 3.45 SQIFVANLQPSNFASQGGIFLLL1168 pKa = 4.47
Molecular weight: 122.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.465
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.528
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.126
Thurlkill 3.516
EMBOSS 3.681
Sillero 3.834
Patrickios 1.113
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A1Q9AT48|A0A1Q9AT48_9RHIZ 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Rhizobium oryzae OX=464029 GN=BJF93_17060 PE=3 SV=1
MM1 pKa = 7.42 FWRR4 pKa = 11.84 SAAFAVAWAVGHH16 pKa = 5.95 ASKK19 pKa = 10.57 RR20 pKa = 11.84 RR21 pKa = 11.84 PATKK25 pKa = 8.71 SAMMTHH31 pKa = 7.01 HH32 pKa = 6.61 VRR34 pKa = 11.84 ILLITRR40 pKa = 11.84 PILPLGKK47 pKa = 10.02 KK48 pKa = 10.15 FKK50 pKa = 10.84 LFLKK54 pKa = 10.3 APRR57 pKa = 11.84 RR58 pKa = 11.84 ASS60 pKa = 3.14
Molecular weight: 6.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.475
IPC2_protein 11.052
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.574
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.31
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4627
0
4627
1480217
28
3204
319.9
34.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.825 ± 0.055
0.76 ± 0.01
5.711 ± 0.032
5.639 ± 0.032
3.78 ± 0.021
8.373 ± 0.031
2.039 ± 0.015
5.345 ± 0.025
3.278 ± 0.029
10.503 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.527 ± 0.017
2.486 ± 0.023
5.069 ± 0.028
3.198 ± 0.021
7.076 ± 0.036
5.564 ± 0.027
5.288 ± 0.035
7.159 ± 0.026
1.229 ± 0.014
2.152 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here