Amino acid dipepetide frequency for Ruminococcaceae bacterium FB2012

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.42AlaAla: 9.42 ± 0.154	1.124AlaCys: 1.124 ± 0.032	5.558AlaAsp: 5.558 ± 0.072	7.364AlaGlu: 7.364 ± 0.101	3.347AlaPhe: 3.347 ± 0.072	6.017AlaGly: 6.017 ± 0.098	1.06AlaHis: 1.06 ± 0.028	4.01AlaIle: 4.01 ± 0.065	4.845AlaLys: 4.845 ± 0.075	7.284AlaLeu: 7.284 ± 0.103	2.106AlaMet: 2.106 ± 0.045	2.484AlaAsn: 2.484 ± 0.058	2.576AlaPro: 2.576 ± 0.063	2.032AlaGln: 2.032 ± 0.052	3.128AlaArg: 3.128 ± 0.056	4.448AlaSer: 4.448 ± 0.07	2.662AlaThr: 2.662 ± 0.061	7.758AlaVal: 7.758 ± 0.125	0.534AlaTrp: 0.534 ± 0.022	2.686AlaTyr: 2.686 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.343CysAla: 1.343 ± 0.041	0.343CysCys: 0.343 ± 0.019	1.063CysAsp: 1.063 ± 0.03	1.172CysGlu: 1.172 ± 0.037	0.797CysPhe: 0.797 ± 0.029	1.799CysGly: 1.799 ± 0.048	0.234CysHis: 0.234 ± 0.014	1.1CysIle: 1.1 ± 0.035	0.808CysLys: 0.808 ± 0.028	1.161CysLeu: 1.161 ± 0.037	0.354CysMet: 0.354 ± 0.019	0.525CysAsn: 0.525 ± 0.022	0.664CysPro: 0.664 ± 0.027	0.272CysGln: 0.272 ± 0.019	0.899CysArg: 0.899 ± 0.031	1.134CysSer: 1.134 ± 0.035	0.918CysThr: 0.918 ± 0.033	1.001CysVal: 1.001 ± 0.032	0.156CysTrp: 0.156 ± 0.012	0.607CysTyr: 0.607 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	4.305AspAla: 4.305 ± 0.061	0.987AspCys: 0.987 ± 0.027	3.856AspAsp: 3.856 ± 0.078	5.185AspGlu: 5.185 ± 0.084	2.843AspPhe: 2.843 ± 0.056	5.115AspGly: 5.115 ± 0.085	0.962AspHis: 0.962 ± 0.028	4.765AspIle: 4.765 ± 0.07	4.396AspLys: 4.396 ± 0.063	4.727AspLeu: 4.727 ± 0.07	1.769AspMet: 1.769 ± 0.04	2.585AspAsn: 2.585 ± 0.064	2.442AspPro: 2.442 ± 0.052	1.128AspGln: 1.128 ± 0.031	3.102AspArg: 3.102 ± 0.066	4.34AspSer: 4.34 ± 0.073	3.513AspThr: 3.513 ± 0.062	3.667AspVal: 3.667 ± 0.054	0.601AspTrp: 0.601 ± 0.024	2.801AspTyr: 2.801 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.424GluAla: 5.424 ± 0.091	0.978GluCys: 0.978 ± 0.032	4.214GluAsp: 4.214 ± 0.078	5.628GluGlu: 5.628 ± 0.116	2.735GluPhe: 2.735 ± 0.048	4.73GluGly: 4.73 ± 0.066	1.247GluHis: 1.247 ± 0.039	5.152GluIle: 5.152 ± 0.076	6.066GluLys: 6.066 ± 0.084	7.291GluLeu: 7.291 ± 0.095	2.117GluMet: 2.117 ± 0.052	3.825GluAsn: 3.825 ± 0.057	2.221GluPro: 2.221 ± 0.062	2.614GluGln: 2.614 ± 0.052	3.581GluArg: 3.581 ± 0.064	3.833GluSer: 3.833 ± 0.078	3.877GluThr: 3.877 ± 0.06	3.783GluVal: 3.783 ± 0.069	0.638GluTrp: 0.638 ± 0.027	3.306GluTyr: 3.306 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.448PheAla: 3.448 ± 0.063	0.85PheCys: 0.85 ± 0.031	3.208PheAsp: 3.208 ± 0.055	3.185PheGlu: 3.185 ± 0.059	1.916PhePhe: 1.916 ± 0.051	3.407PheGly: 3.407 ± 0.061	0.537PheHis: 0.537 ± 0.022	3.033PheIle: 3.033 ± 0.054	2.391PheLys: 2.391 ± 0.055	3.166PheLeu: 3.166 ± 0.058	1.146PheMet: 1.146 ± 0.035	1.514PheAsn: 1.514 ± 0.038	1.385PhePro: 1.385 ± 0.037	0.734PheGln: 0.734 ± 0.025	2.042PheArg: 2.042 ± 0.046	3.253PheSer: 3.253 ± 0.061	2.744PheThr: 2.744 ± 0.054	2.894PheVal: 2.894 ± 0.055	0.365PheTrp: 0.365 ± 0.019	1.715PheTyr: 1.715 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	5.185GlyAla: 5.185 ± 0.081	1.437GlyCys: 1.437 ± 0.039	4.084GlyAsp: 4.084 ± 0.065	5.2GlyGlu: 5.2 ± 0.072	3.5GlyPhe: 3.5 ± 0.059	5.428GlyGly: 5.428 ± 0.101	1.208GlyHis: 1.208 ± 0.038	5.562GlyIle: 5.562 ± 0.082	5.404GlyLys: 5.404 ± 0.07	5.948GlyLeu: 5.948 ± 0.089	2.259GlyMet: 2.259 ± 0.039	2.879GlyAsn: 2.879 ± 0.064	1.082GlyPro: 1.082 ± 0.034	1.743GlyGln: 1.743 ± 0.046	3.651GlyArg: 3.651 ± 0.063	4.876GlySer: 4.876 ± 0.079	4.414GlyThr: 4.414 ± 0.074	4.758GlyVal: 4.758 ± 0.073	0.764GlyTrp: 0.764 ± 0.028	3.197GlyTyr: 3.197 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	0.992HisAla: 0.992 ± 0.037	0.313HisCys: 0.313 ± 0.015	0.922HisAsp: 0.922 ± 0.032	0.936HisGlu: 0.936 ± 0.032	0.842HisPhe: 0.842 ± 0.032	1.172HisGly: 1.172 ± 0.036	0.37HisHis: 0.37 ± 0.027	1.284HisIle: 1.284 ± 0.037	0.953HisLys: 0.953 ± 0.027	1.154HisLeu: 1.154 ± 0.033	0.32HisMet: 0.32 ± 0.016	0.653HisAsn: 0.653 ± 0.024	0.749HisPro: 0.749 ± 0.026	0.367HisGln: 0.367 ± 0.018	0.851HisArg: 0.851 ± 0.028	1.071HisSer: 1.071 ± 0.035	0.945HisThr: 0.945 ± 0.029	0.641HisVal: 0.641 ± 0.027	0.147HisTrp: 0.147 ± 0.011	0.733HisTyr: 0.733 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.531IleAla: 5.531 ± 0.073	1.205IleCys: 1.205 ± 0.036	4.37IleAsp: 4.37 ± 0.069	4.348IleGlu: 4.348 ± 0.072	2.71IlePhe: 2.71 ± 0.052	4.966IleGly: 4.966 ± 0.082	1.013IleHis: 1.013 ± 0.031	4.845IleIle: 4.845 ± 0.078	4.387IleLys: 4.387 ± 0.067	5.213IleLeu: 5.213 ± 0.079	1.799IleMet: 1.799 ± 0.047	3.014IleAsn: 3.014 ± 0.059	2.972IlePro: 2.972 ± 0.054	1.357IleGln: 1.357 ± 0.033	3.48IleArg: 3.48 ± 0.071	5.202IleSer: 5.202 ± 0.072	4.505IleThr: 4.505 ± 0.069	4.474IleVal: 4.474 ± 0.07	0.517IleTrp: 0.517 ± 0.022	2.567IleTyr: 2.567 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.865LysAla: 5.865 ± 0.085	0.807LysCys: 0.807 ± 0.026	4.164LysAsp: 4.164 ± 0.074	5.006LysGlu: 5.006 ± 0.081	2.037LysPhe: 2.037 ± 0.051	4.267LysGly: 4.267 ± 0.063	1.134LysHis: 1.134 ± 0.03	4.219LysIle: 4.219 ± 0.064	5.362LysLys: 5.362 ± 0.089	6.372LysLeu: 6.372 ± 0.081	1.889LysMet: 1.889 ± 0.043	3.3LysAsn: 3.3 ± 0.066	2.308LysPro: 2.308 ± 0.046	2.075LysGln: 2.075 ± 0.049	3.316LysArg: 3.316 ± 0.058	3.546LysSer: 3.546 ± 0.062	3.883LysThr: 3.883 ± 0.07	4.038LysVal: 4.038 ± 0.066	0.598LysTrp: 0.598 ± 0.024	2.849LysTyr: 2.849 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	6.879LeuAla: 6.879 ± 0.09	1.712LeuCys: 1.712 ± 0.046	5.403LeuAsp: 5.403 ± 0.073	5.652LeuGlu: 5.652 ± 0.083	3.723LeuPhe: 3.723 ± 0.08	5.852LeuGly: 5.852 ± 0.079	1.359LeuHis: 1.359 ± 0.039	6.077LeuIle: 6.077 ± 0.09	5.649LeuLys: 5.649 ± 0.073	7.592LeuLeu: 7.592 ± 0.117	2.476LeuMet: 2.476 ± 0.049	3.438LeuAsn: 3.438 ± 0.06	3.497LeuPro: 3.497 ± 0.065	1.627LeuGln: 1.627 ± 0.04	4.265LeuArg: 4.265 ± 0.08	6.563LeuSer: 6.563 ± 0.091	5.675LeuThr: 5.675 ± 0.086	4.72LeuVal: 4.72 ± 0.075	0.686LeuTrp: 0.686 ± 0.026	3.075LeuTyr: 3.075 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	1.74MetAla: 1.74 ± 0.044	0.361MetCys: 0.361 ± 0.017	1.398MetAsp: 1.398 ± 0.042	1.424MetGlu: 1.424 ± 0.038	1.113MetPhe: 1.113 ± 0.031	1.621MetGly: 1.621 ± 0.038	0.408MetHis: 0.408 ± 0.018	2.133MetIle: 2.133 ± 0.041	2.413MetLys: 2.413 ± 0.05	3.044MetLeu: 3.044 ± 0.065	0.822MetMet: 0.822 ± 0.026	1.422MetAsn: 1.422 ± 0.033	1.13MetPro: 1.13 ± 0.035	0.818MetGln: 0.818 ± 0.022	1.309MetArg: 1.309 ± 0.037	1.824MetSer: 1.824 ± 0.043	1.886MetThr: 1.886 ± 0.041	1.262MetVal: 1.262 ± 0.034	0.207MetTrp: 0.207 ± 0.013	0.886MetTyr: 0.886 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.272AsnAla: 3.272 ± 0.061	0.743AsnCys: 0.743 ± 0.029	2.667AsnAsp: 2.667 ± 0.057	2.781AsnGlu: 2.781 ± 0.052	1.498AsnPhe: 1.498 ± 0.037	3.824AsnGly: 3.824 ± 0.069	0.555AsnHis: 0.555 ± 0.022	3.285AsnIle: 3.285 ± 0.063	2.532AsnLys: 2.532 ± 0.057	2.79AsnLeu: 2.79 ± 0.049	1.191AsnMet: 1.191 ± 0.035	2.037AsnAsn: 2.037 ± 0.072	1.557AsnPro: 1.557 ± 0.038	0.853AsnGln: 0.853 ± 0.027	1.735AsnArg: 1.735 ± 0.043	2.732AsnSer: 2.732 ± 0.06	2.415AsnThr: 2.415 ± 0.047	2.809AsnVal: 2.809 ± 0.063	0.409AsnTrp: 0.409 ± 0.019	1.799AsnTyr: 1.799 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	3.077ProAla: 3.077 ± 0.063	0.511ProCys: 0.511 ± 0.024	2.85ProAsp: 2.85 ± 0.061	3.755ProGlu: 3.755 ± 0.069	1.505ProPhe: 1.505 ± 0.035	1.969ProGly: 1.969 ± 0.043	0.505ProHis: 0.505 ± 0.024	1.904ProIle: 1.904 ± 0.039	2.102ProLys: 2.102 ± 0.038	2.711ProLeu: 2.711 ± 0.05	0.855ProMet: 0.855 ± 0.026	1.224ProAsn: 1.224 ± 0.039	1.007ProPro: 1.007 ± 0.036	1.018ProGln: 1.018 ± 0.032	1.224ProArg: 1.224 ± 0.037	2.023ProSer: 2.023 ± 0.043	1.622ProThr: 1.622 ± 0.039	2.904ProVal: 2.904 ± 0.053	0.265ProTrp: 0.265 ± 0.016	1.393ProTyr: 1.393 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	1.886GlnAla: 1.886 ± 0.048	0.284GlnCys: 0.284 ± 0.017	1.139GlnAsp: 1.139 ± 0.029	1.48GlnGlu: 1.48 ± 0.04	1.055GlnPhe: 1.055 ± 0.032	1.612GlnGly: 1.612 ± 0.038	0.432GlnHis: 0.432 ± 0.02	1.789GlnIle: 1.789 ± 0.045	1.814GlnLys: 1.814 ± 0.042	2.45GlnLeu: 2.45 ± 0.049	0.773GlnMet: 0.773 ± 0.031	1.184GlnAsn: 1.184 ± 0.038	0.881GlnPro: 0.881 ± 0.039	0.972GlnGln: 0.972 ± 0.068	1.306GlnArg: 1.306 ± 0.032	1.463GlnSer: 1.463 ± 0.033	1.499GlnThr: 1.499 ± 0.044	1.384GlnVal: 1.384 ± 0.037	0.216GlnTrp: 0.216 ± 0.015	1.107GlnTyr: 1.107 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	3.206ArgAla: 3.206 ± 0.061	0.748ArgCys: 0.748 ± 0.027	2.509ArgAsp: 2.509 ± 0.045	3.691ArgGlu: 3.691 ± 0.074	2.258ArgPhe: 2.258 ± 0.046	2.752ArgGly: 2.752 ± 0.056	0.812ArgHis: 0.812 ± 0.029	3.683ArgIle: 3.683 ± 0.065	3.204ArgLys: 3.204 ± 0.062	4.675ArgLeu: 4.675 ± 0.088	1.385ArgMet: 1.385 ± 0.036	1.933ArgAsn: 1.933 ± 0.044	1.498ArgPro: 1.498 ± 0.04	1.464ArgGln: 1.464 ± 0.043	2.807ArgArg: 2.807 ± 0.072	3.116ArgSer: 3.116 ± 0.059	2.505ArgThr: 2.505 ± 0.051	2.716ArgVal: 2.716 ± 0.05	0.413ArgTrp: 0.413 ± 0.019	2.009ArgTyr: 2.009 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	5.732SerAla: 5.732 ± 0.088	1.053SerCys: 1.053 ± 0.032	4.529SerAsp: 4.529 ± 0.075	5.359SerGlu: 5.359 ± 0.08	3.174SerPhe: 3.174 ± 0.053	5.769SerGly: 5.769 ± 0.081	0.876SerHis: 0.876 ± 0.027	3.818SerIle: 3.818 ± 0.076	3.962SerLys: 3.962 ± 0.068	5.432SerLeu: 5.432 ± 0.072	1.554SerMet: 1.554 ± 0.041	2.205SerAsn: 2.205 ± 0.053	2.033SerPro: 2.033 ± 0.041	1.625SerGln: 1.625 ± 0.039	3.091SerArg: 3.091 ± 0.053	4.736SerSer: 4.736 ± 0.098	3.041SerThr: 3.041 ± 0.071	5.038SerVal: 5.038 ± 0.07	0.57SerTrp: 0.57 ± 0.025	2.546SerTyr: 2.546 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	5.832ThrAla: 5.832 ± 0.092	0.682ThrCys: 0.682 ± 0.026	4.084ThrAsp: 4.084 ± 0.069	4.327ThrGlu: 4.327 ± 0.064	2.349ThrPhe: 2.349 ± 0.049	4.586ThrGly: 4.586 ± 0.078	0.831ThrHis: 0.831 ± 0.026	3.006ThrIle: 3.006 ± 0.067	2.915ThrLys: 2.915 ± 0.055	4.918ThrLeu: 4.918 ± 0.081	1.231ThrMet: 1.231 ± 0.031	1.877ThrAsn: 1.877 ± 0.053	2.245ThrPro: 2.245 ± 0.045	1.252ThrGln: 1.252 ± 0.036	1.978ThrArg: 1.978 ± 0.042	3.32ThrSer: 3.32 ± 0.08	3.128ThrThr: 3.128 ± 0.116	6.051ThrVal: 6.051 ± 0.095	0.437ThrTrp: 0.437 ± 0.022	2.114ThrTyr: 2.114 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	3.909ValAla: 3.909 ± 0.068	1.326ValCys: 1.326 ± 0.038	3.549ValAsp: 3.549 ± 0.063	3.647ValGlu: 3.647 ± 0.064	3.303ValPhe: 3.303 ± 0.054	4.143ValGly: 4.143 ± 0.069	0.987ValHis: 0.987 ± 0.031	5.49ValIle: 5.49 ± 0.08	4.777ValLys: 4.777 ± 0.079	5.872ValLeu: 5.872 ± 0.075	1.985ValMet: 1.985 ± 0.04	2.93ValAsn: 2.93 ± 0.066	2.648ValPro: 2.648 ± 0.052	1.456ValGln: 1.456 ± 0.035	3.272ValArg: 3.272 ± 0.065	5.194ValSer: 5.194 ± 0.077	4.892ValThr: 4.892 ± 0.087	3.911ValVal: 3.911 ± 0.082	0.611ValTrp: 0.611 ± 0.025	2.777ValTyr: 2.777 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.549TrpAla: 0.549 ± 0.027	0.185TrpCys: 0.185 ± 0.015	0.589TrpAsp: 0.589 ± 0.025	0.557TrpGlu: 0.557 ± 0.024	0.448TrpPhe: 0.448 ± 0.025	0.632TrpGly: 0.632 ± 0.023	0.182TrpHis: 0.182 ± 0.015	0.53TrpIle: 0.53 ± 0.025	0.55TrpLys: 0.55 ± 0.024	0.834TrpLeu: 0.834 ± 0.028	0.219TrpMet: 0.219 ± 0.012	0.52TrpAsn: 0.52 ± 0.027	0.158TrpPro: 0.158 ± 0.011	0.328TrpGln: 0.328 ± 0.017	0.35TrpArg: 0.35 ± 0.017	0.535TrpSer: 0.535 ± 0.022	0.439TrpThr: 0.439 ± 0.021	0.482TrpVal: 0.482 ± 0.018	0.097TrpTrp: 0.097 ± 0.008	0.427TrpTyr: 0.427 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.848TyrAla: 2.848 ± 0.06	0.702TyrCys: 0.702 ± 0.025	2.933TyrAsp: 2.933 ± 0.063	2.559TyrGlu: 2.559 ± 0.046	1.778TyrPhe: 1.778 ± 0.045	2.921TyrGly: 2.921 ± 0.067	0.703TyrHis: 0.703 ± 0.025	2.79TyrIle: 2.79 ± 0.051	2.506TyrLys: 2.506 ± 0.047	3.284TyrLeu: 3.284 ± 0.059	0.966TyrMet: 0.966 ± 0.025	1.934TyrAsn: 1.934 ± 0.042	1.371TyrPro: 1.371 ± 0.038	0.991TyrGln: 0.991 ± 0.03	2.0TyrArg: 2.0 ± 0.047	2.934TyrSer: 2.934 ± 0.054	2.603TyrThr: 2.603 ± 0.061	2.39TyrVal: 2.39 ± 0.051	0.382TyrTrp: 0.382 ± 0.021	2.07TyrTyr: 2.07 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3207 proteins (1098134 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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