Beihai sobemo-like virus 5
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEA0|A0A1L3KEA0_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 5 OX=1922702 PE=4 SV=1
MM1 pKa = 7.69 ALLLSRR7 pKa = 11.84 VQGADD12 pKa = 2.94 TKK14 pKa = 10.84 AVKK17 pKa = 10.29 KK18 pKa = 10.57 VVTSFGLEE26 pKa = 3.66 PGAARR31 pKa = 11.84 AYY33 pKa = 10.56 LHH35 pKa = 6.84 LGLLFGIYY43 pKa = 10.29 KK44 pKa = 9.2 LVCARR49 pKa = 11.84 KK50 pKa = 8.91 EE51 pKa = 3.91 LCPPIARR58 pKa = 11.84 ACLRR62 pKa = 11.84 VLYY65 pKa = 9.55 YY66 pKa = 9.36 VVKK69 pKa = 9.48 MSSPGVSLVSWLKK82 pKa = 9.44 EE83 pKa = 3.39 RR84 pKa = 11.84 LRR86 pKa = 11.84 RR87 pKa = 11.84 GRR89 pKa = 11.84 IADD92 pKa = 4.0 CVQGGPPTKK101 pKa = 9.9 HH102 pKa = 6.8 GYY104 pKa = 9.14 FRR106 pKa = 11.84 WNGKK110 pKa = 9.96 DD111 pKa = 3.63 FVRR114 pKa = 11.84 HH115 pKa = 5.34 SDD117 pKa = 3.34 EE118 pKa = 4.49 SISRR122 pKa = 11.84 VKK124 pKa = 9.93 MLRR127 pKa = 11.84 GTTTIDD133 pKa = 3.02 AYY135 pKa = 10.38 GQEE138 pKa = 4.02 STAYY142 pKa = 8.56 NWEE145 pKa = 3.82 ILEE148 pKa = 4.06 PARR151 pKa = 11.84 PKK153 pKa = 10.67 DD154 pKa = 3.65 LSPGEE159 pKa = 4.19 SALEE163 pKa = 4.05 GLSSNARR170 pKa = 11.84 PSPLEE175 pKa = 4.0 KK176 pKa = 10.6 GHH178 pKa = 6.18 VLVHH182 pKa = 6.97 DD183 pKa = 5.18 SILNLFSTGLVLNNILIMSRR203 pKa = 11.84 HH204 pKa = 4.61 KK205 pKa = 9.45 TASLTHH211 pKa = 5.78 LVVRR215 pKa = 11.84 GTNTTAVAGRR225 pKa = 11.84 QSGVRR230 pKa = 11.84 ISLDD234 pKa = 2.85 RR235 pKa = 11.84 AYY237 pKa = 11.35 YY238 pKa = 10.69 LDD240 pKa = 5.66 DD241 pKa = 4.41 FGAPKK246 pKa = 9.53 WDD248 pKa = 4.14 EE249 pKa = 4.0 KK250 pKa = 11.21 RR251 pKa = 11.84 HH252 pKa = 5.02 TCTFLDD258 pKa = 4.92 FMAIPLDD265 pKa = 3.63 KK266 pKa = 11.3 DD267 pKa = 4.02 EE268 pKa = 5.66 ISMIGVKK275 pKa = 8.74 SLKK278 pKa = 10.83 DD279 pKa = 3.21 KK280 pKa = 11.08 DD281 pKa = 3.11 ITRR284 pKa = 11.84 NYY286 pKa = 8.76 EE287 pKa = 3.93 LEE289 pKa = 4.5 RR290 pKa = 11.84 GTITYY295 pKa = 8.1 ATDD298 pKa = 3.42 DD299 pKa = 3.73 FGNVVKK305 pKa = 10.74 EE306 pKa = 4.04 EE307 pKa = 4.15 GSIPEE312 pKa = 4.36 AEE314 pKa = 4.07 LHH316 pKa = 5.42 IHH318 pKa = 6.3 NLGLALAHH326 pKa = 6.73 IVSQPGASSSGVGVVQNGYY345 pKa = 10.03 KK346 pKa = 10.21 LAGMWLGQPAHH357 pKa = 6.15 SLKK360 pKa = 10.27 KK361 pKa = 10.16 HH362 pKa = 5.84 RR363 pKa = 11.84 GKK365 pKa = 10.97 ANLFMHH371 pKa = 7.04 TDD373 pKa = 3.69 AIMANLEE380 pKa = 3.85 QMGLYY385 pKa = 9.37 KK386 pKa = 10.56 HH387 pKa = 6.28 PLIEE391 pKa = 6.31 RR392 pKa = 11.84 IQQWSDD398 pKa = 2.92 SLQTPTSLPEE408 pKa = 3.91 APGEE412 pKa = 4.07 SRR414 pKa = 11.84 EE415 pKa = 4.34 SKK417 pKa = 10.43 KK418 pKa = 10.46 EE419 pKa = 3.32 RR420 pKa = 11.84 AARR423 pKa = 11.84 KK424 pKa = 7.77 WFEE427 pKa = 3.87 YY428 pKa = 8.17 YY429 pKa = 9.75 QEE431 pKa = 4.58 YY432 pKa = 10.6 KK433 pKa = 10.75 DD434 pKa = 3.9 EE435 pKa = 4.89 PYY437 pKa = 11.02 EE438 pKa = 4.27 AMEE441 pKa = 4.15 EE442 pKa = 4.2 DD443 pKa = 3.89 EE444 pKa = 4.35 EE445 pKa = 4.4 EE446 pKa = 4.49 EE447 pKa = 4.29 YY448 pKa = 11.16 EE449 pKa = 4.24 KK450 pKa = 11.23 VYY452 pKa = 10.72 GRR454 pKa = 11.84 SGHH457 pKa = 6.78 ARR459 pKa = 11.84 TQPAVALPKK468 pKa = 10.39 AVAAPGPRR476 pKa = 11.84 IPPRR480 pKa = 11.84 ARR482 pKa = 11.84 HH483 pKa = 5.84 PGEE486 pKa = 4.12 SAAEE490 pKa = 3.82 RR491 pKa = 11.84 ALEE494 pKa = 3.81 ITRR497 pKa = 11.84 QNMLLDD503 pKa = 3.34 IDD505 pKa = 4.98 RR506 pKa = 11.84 NVKK509 pKa = 8.06 SWKK512 pKa = 10.05 YY513 pKa = 10.61 GILHH517 pKa = 6.41 EE518 pKa = 4.31 EE519 pKa = 4.06 APRR522 pKa = 11.84 VLRR525 pKa = 11.84 EE526 pKa = 3.25 ISYY529 pKa = 10.23 YY530 pKa = 10.32 RR531 pKa = 11.84 EE532 pKa = 3.58 EE533 pKa = 3.82 ATADD537 pKa = 3.06 VRR539 pKa = 11.84 EE540 pKa = 4.07 RR541 pKa = 11.84 LKK543 pKa = 11.14 AVLATPSNALLRR555 pKa = 11.84 DD556 pKa = 3.65 YY557 pKa = 9.51 MKK559 pKa = 10.36 KK560 pKa = 10.21 VSPMWSPGEE569 pKa = 3.92 EE570 pKa = 4.2 GEE572 pKa = 5.09 EE573 pKa = 3.86 ILDD576 pKa = 3.94 RR577 pKa = 11.84 DD578 pKa = 3.65 GNPYY582 pKa = 9.25 FRR584 pKa = 11.84 RR585 pKa = 11.84 VGSYY589 pKa = 10.63 EE590 pKa = 3.62 PTRR593 pKa = 11.84 TAKK596 pKa = 9.76 AKK598 pKa = 10.15 KK599 pKa = 9.36 YY600 pKa = 10.2 RR601 pKa = 11.84 EE602 pKa = 4.22 EE603 pKa = 3.89 EE604 pKa = 3.56 TDD606 pKa = 3.25 LQKK609 pKa = 11.0 RR610 pKa = 11.84 LRR612 pKa = 11.84 EE613 pKa = 3.93 LANRR617 pKa = 11.84 YY618 pKa = 8.81 YY619 pKa = 11.07 ADD621 pKa = 4.24 KK622 pKa = 11.28 LHH624 pKa = 6.21 GCKK627 pKa = 9.88 KK628 pKa = 10.0 GEE630 pKa = 3.82 YY631 pKa = 9.57 RR632 pKa = 11.84 IPTSSKK638 pKa = 10.48 KK639 pKa = 10.85 NIDD642 pKa = 3.6 RR643 pKa = 11.84 SLKK646 pKa = 9.86 AQAALAVAGPPQLTSEE662 pKa = 4.1 QEE664 pKa = 4.23 EE665 pKa = 4.32 EE666 pKa = 4.04 FEE668 pKa = 4.02 AAVRR672 pKa = 11.84 LVRR675 pKa = 11.84 EE676 pKa = 4.32 KK677 pKa = 10.87 YY678 pKa = 9.57 KK679 pKa = 11.02 AGIGDD684 pKa = 4.19 EE685 pKa = 4.45 PIKK688 pKa = 10.2 TYY690 pKa = 10.92 LEE692 pKa = 4.13 EE693 pKa = 5.78 GDD695 pKa = 3.83 YY696 pKa = 11.64 GFLKK700 pKa = 10.14 TFLGFEE706 pKa = 4.53 DD707 pKa = 4.05 KK708 pKa = 11.14 SSGVSARR715 pKa = 11.84 FRR717 pKa = 11.84 NMKK720 pKa = 8.88 KK721 pKa = 9.9 AAWVKK726 pKa = 9.05 AHH728 pKa = 6.82 PEE730 pKa = 4.04 EE731 pKa = 4.65 VVDD734 pKa = 4.61 LALSRR739 pKa = 11.84 LILIAVAGDD748 pKa = 3.4 QLKK751 pKa = 10.75 DD752 pKa = 3.46 LNAIEE757 pKa = 5.11 LIQYY761 pKa = 9.38 GCADD765 pKa = 3.47 VKK767 pKa = 11.17 DD768 pKa = 3.9 IFMKK772 pKa = 10.63 PEE774 pKa = 3.47 EE775 pKa = 4.68 HH776 pKa = 6.58 SPQKK780 pKa = 9.89 IAEE783 pKa = 4.11 GRR785 pKa = 11.84 FRR787 pKa = 11.84 LIWISSLIDD796 pKa = 3.4 LTVQALLHH804 pKa = 6.2 KK805 pKa = 10.06 ADD807 pKa = 3.91 NAAHH811 pKa = 5.94 VDD813 pKa = 3.84 AYY815 pKa = 10.79 QAGHH819 pKa = 6.38 LTCAALGMGHH829 pKa = 6.05 SPEE832 pKa = 4.33 GLQHH836 pKa = 6.79 LVRR839 pKa = 11.84 AFEE842 pKa = 4.23 RR843 pKa = 11.84 EE844 pKa = 4.08 GVAEE848 pKa = 4.29 RR849 pKa = 11.84 NVSSDD854 pKa = 2.72 ASAFDD859 pKa = 3.97 LSIDD863 pKa = 3.47 GSFIHH868 pKa = 7.52 ADD870 pKa = 3.44 GEE872 pKa = 4.27 RR873 pKa = 11.84 RR874 pKa = 11.84 ADD876 pKa = 3.3 NCADD880 pKa = 3.62 EE881 pKa = 5.26 DD882 pKa = 3.87 VARR885 pKa = 11.84 LVKK888 pKa = 10.27 RR889 pKa = 11.84 YY890 pKa = 9.69 AHH892 pKa = 6.77 ILCSHH897 pKa = 6.47 VLNNHH902 pKa = 5.23 GEE904 pKa = 3.85 VWLCLKK910 pKa = 10.95 YY911 pKa = 10.76 GVTSSGQLSTTTQNTFSRR929 pKa = 11.84 SVMAAYY935 pKa = 9.63 GGCLGWTCAGDD946 pKa = 4.29 DD947 pKa = 4.41 LVGDD951 pKa = 4.22 EE952 pKa = 5.03 NFDD955 pKa = 3.93 HH956 pKa = 7.04 NRR958 pKa = 11.84 LLHH961 pKa = 5.79 FGVRR965 pKa = 11.84 SRR967 pKa = 11.84 DD968 pKa = 3.52 VEE970 pKa = 4.29 LHH972 pKa = 5.49 VGEE975 pKa = 5.35 ADD977 pKa = 3.83 FTSHH981 pKa = 8.15 LINTSTATAAFGNVEE996 pKa = 3.74 KK997 pKa = 10.25 MLWHH1001 pKa = 7.25 LYY1003 pKa = 7.6 DD1004 pKa = 3.16 TCTDD1008 pKa = 2.79 VSTNRR1013 pKa = 11.84 EE1014 pKa = 3.88 RR1015 pKa = 11.84 FGSSLYY1021 pKa = 10.26 ILRR1024 pKa = 11.84 DD1025 pKa = 3.6 TPGVLEE1031 pKa = 5.08 DD1032 pKa = 3.52 VTAITNDD1039 pKa = 3.27 FGINTDD1045 pKa = 3.41 GYY1047 pKa = 9.35 VAEE1050 pKa = 4.62 SSLIRR1055 pKa = 11.84 DD1056 pKa = 4.03 LAA1058 pKa = 5.32
Molecular weight: 118.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.112
IPC2_protein 6.046
IPC_protein 6.122
Toseland 6.517
ProMoST 6.517
Dawson 6.427
Bjellqvist 6.427
Wikipedia 6.453
Rodwell 6.427
Grimsley 6.678
Solomon 6.44
Lehninger 6.427
Nozaki 6.722
DTASelect 6.898
Thurlkill 6.927
EMBOSS 6.883
Sillero 6.825
Patrickios 4.317
IPC_peptide 6.453
IPC2_peptide 6.737
IPC2.peptide.svr19 6.724
Protein with the highest isoelectric point:
>tr|A0A1L3KEA0|A0A1L3KEA0_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 5 OX=1922702 PE=4 SV=1
MM1 pKa = 7.3 VSPGKK6 pKa = 9.88 RR7 pKa = 11.84 VKK9 pKa = 9.85 RR10 pKa = 11.84 TIRR13 pKa = 11.84 KK14 pKa = 8.73 QKK16 pKa = 10.44 AKK18 pKa = 10.34 VIKK21 pKa = 10.38 DD22 pKa = 3.31 VTLLRR27 pKa = 11.84 GIKK30 pKa = 9.65 QGVGMVVKK38 pKa = 10.52 RR39 pKa = 11.84 PFGSAKK45 pKa = 9.9 ARR47 pKa = 11.84 PRR49 pKa = 11.84 RR50 pKa = 11.84 TANRR54 pKa = 11.84 GRR56 pKa = 11.84 NISKK60 pKa = 10.48 KK61 pKa = 10.33 AALCALTNAHH71 pKa = 6.62 LPLPRR76 pKa = 11.84 AVGAYY81 pKa = 8.15 TVVKK85 pKa = 7.22 TTTVIDD91 pKa = 3.75 SASRR95 pKa = 11.84 AMLFGTIKK103 pKa = 10.62 GNRR106 pKa = 11.84 SNHH109 pKa = 6.9 PDD111 pKa = 3.57 PAWYY115 pKa = 8.21 DD116 pKa = 3.95 TVAVTAGTSSTTAVGAADD134 pKa = 3.51 NAKK137 pKa = 10.27 FYY139 pKa = 11.07 LDD141 pKa = 3.44 TALASSGFASCRR153 pKa = 11.84 LVPAAFTVQVMCPKK167 pKa = 10.39 SLQTADD173 pKa = 3.76 GIVYY177 pKa = 10.28 IGRR180 pKa = 11.84 CKK182 pKa = 10.12 QVLDD186 pKa = 3.9 LMGNTRR192 pKa = 11.84 TWTTVMDD199 pKa = 4.62 DD200 pKa = 3.66 LVSYY204 pKa = 10.31 SSPRR208 pKa = 11.84 LCSAGKK214 pKa = 9.54 LALRR218 pKa = 11.84 GVKK221 pKa = 9.8 VDD223 pKa = 5.31 AIPNNMSQLSDD234 pKa = 3.67 FCPRR238 pKa = 11.84 AILTSSDD245 pKa = 3.34 TNKK248 pKa = 8.09 WTWGQGGVDD257 pKa = 3.32 AQFEE261 pKa = 4.47 GFAPIFVYY269 pKa = 10.67 NKK271 pKa = 8.71 NTVDD275 pKa = 3.58 LQYY278 pKa = 11.29 LVTVEE283 pKa = 3.4 WRR285 pKa = 11.84 VRR287 pKa = 11.84 FDD289 pKa = 3.68 PGNPAYY295 pKa = 10.13 AGHH298 pKa = 6.56 TFHH301 pKa = 7.23 PVSSDD306 pKa = 3.62 TCWSEE311 pKa = 3.83 TLKK314 pKa = 11.07 GMEE317 pKa = 4.18 AEE319 pKa = 4.18 GHH321 pKa = 5.7 GVVDD325 pKa = 4.46 LAEE328 pKa = 5.24 GIADD332 pKa = 4.04 FGDD335 pKa = 3.58 AAMTAVGALLL345 pKa = 3.8
Molecular weight: 37.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.077
IPC2_protein 8.975
IPC_protein 8.916
Toseland 9.736
ProMoST 9.428
Dawson 9.955
Bjellqvist 9.663
Wikipedia 10.101
Rodwell 10.35
Grimsley 10.014
Solomon 9.984
Lehninger 9.955
Nozaki 9.867
DTASelect 9.619
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.897
Patrickios 9.575
IPC_peptide 9.984
IPC2_peptide 8.419
IPC2.peptide.svr19 7.855
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1403
345
1058
701.5
77.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.979 ± 0.846
1.497 ± 0.279
5.845 ± 0.025
6.415 ± 2.447
2.922 ± 0.291
7.627 ± 0.408
2.851 ± 0.734
4.277 ± 0.418
5.916 ± 0.09
9.337 ± 1.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.996 ± 0.321
3.35 ± 0.219
4.205 ± 0.226
2.495 ± 0.06
6.771 ± 0.358
6.842 ± 0.092
5.845 ± 1.492
6.985 ± 1.351
1.426 ± 0.164
3.421 ± 0.577
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here