Neisseria sicca ATCC 29256
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3646 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6MAQ8|C6MAQ8_NEISI Uncharacterized protein OS=Neisseria sicca ATCC 29256 OX=547045 GN=NEISICOT_03640 PE=4 SV=1
MM1 pKa = 7.33 NLDD4 pKa = 2.82 KK5 pKa = 10.66 FYY7 pKa = 10.87 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.9 ISISEE15 pKa = 4.36 VIDD18 pKa = 3.58 CARR21 pKa = 11.84 LFNDD25 pKa = 4.42 DD26 pKa = 4.57 DD27 pKa = 3.82 TDD29 pKa = 3.69 KK30 pKa = 10.82 FAKK33 pKa = 10.07 FPYY36 pKa = 9.7 IYY38 pKa = 9.94 ISSDD42 pKa = 3.11 EE43 pKa = 4.66 DD44 pKa = 3.6 EE45 pKa = 5.52 SILDD49 pKa = 4.2 LEE51 pKa = 4.91 TVCYY55 pKa = 10.3 LDD57 pKa = 4.26 NGPKK61 pKa = 10.05 IMDD64 pKa = 3.96 GKK66 pKa = 10.79 EE67 pKa = 3.75 VFPDD71 pKa = 4.4 FVNEE75 pKa = 3.87 NGLDD79 pKa = 3.6 ILCSGNVFMDD89 pKa = 3.76 VYY91 pKa = 11.51 DD92 pKa = 5.11 DD93 pKa = 4.26 LDD95 pKa = 3.34 EE96 pKa = 5.43 RR97 pKa = 11.84 YY98 pKa = 10.06 PNFTKK103 pKa = 10.7 DD104 pKa = 3.37 QFIDD108 pKa = 3.28 ALNYY112 pKa = 10.97 YY113 pKa = 8.88 MDD115 pKa = 4.43 NDD117 pKa = 4.43 DD118 pKa = 4.49 YY119 pKa = 11.82 IEE121 pKa = 3.92 FF122 pKa = 4.64
Molecular weight: 14.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.528
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.592
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.164
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.884
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|C6M575|C6M575_NEISI Uncharacterized protein OS=Neisseria sicca ATCC 29256 OX=547045 GN=NEISICOT_01675 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSVTKK11 pKa = 10.56 RR12 pKa = 11.84 KK13 pKa = 7.91 RR14 pKa = 11.84 THH16 pKa = 5.89 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3646
0
3646
821177
34
3862
225.2
25.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.72 ± 0.067
1.131 ± 0.017
5.315 ± 0.041
6.041 ± 0.057
4.445 ± 0.035
7.25 ± 0.059
2.218 ± 0.024
5.777 ± 0.042
5.663 ± 0.04
9.873 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.531 ± 0.025
4.229 ± 0.049
4.226 ± 0.037
4.289 ± 0.032
5.369 ± 0.049
5.699 ± 0.033
5.208 ± 0.043
6.627 ± 0.045
1.291 ± 0.021
3.099 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here