Neisseria sicca ATCC 29256 
Average proteome isoelectric point is 7.06 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 3646 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|C6MAQ8|C6MAQ8_NEISI Uncharacterized protein OS=Neisseria sicca ATCC 29256 OX=547045 GN=NEISICOT_03640 PE=4 SV=1MM1 pKa = 7.33  NLDD4 pKa = 2.82  KK5 pKa = 10.66  FYY7 pKa = 10.87  RR8 pKa = 11.84  RR9 pKa = 11.84  KK10 pKa = 9.9  ISISEE15 pKa = 4.36  VIDD18 pKa = 3.58  CARR21 pKa = 11.84  LFNDD25 pKa = 4.42  DD26 pKa = 4.57  DD27 pKa = 3.82  TDD29 pKa = 3.69  KK30 pKa = 10.82  FAKK33 pKa = 10.07  FPYY36 pKa = 9.7  IYY38 pKa = 9.94  ISSDD42 pKa = 3.11  EE43 pKa = 4.66  DD44 pKa = 3.6  EE45 pKa = 5.52  SILDD49 pKa = 4.2  LEE51 pKa = 4.91  TVCYY55 pKa = 10.3  LDD57 pKa = 4.26  NGPKK61 pKa = 10.05  IMDD64 pKa = 3.96  GKK66 pKa = 10.79  EE67 pKa = 3.75  VFPDD71 pKa = 4.4  FVNEE75 pKa = 3.87  NGLDD79 pKa = 3.6  ILCSGNVFMDD89 pKa = 3.76  VYY91 pKa = 11.51  DD92 pKa = 5.11  DD93 pKa = 4.26  LDD95 pKa = 3.34  EE96 pKa = 5.43  RR97 pKa = 11.84  YY98 pKa = 10.06  PNFTKK103 pKa = 10.7  DD104 pKa = 3.37  QFIDD108 pKa = 3.28  ALNYY112 pKa = 10.97  YY113 pKa = 8.88  MDD115 pKa = 4.43  NDD117 pKa = 4.43  DD118 pKa = 4.49  YY119 pKa = 11.82  IEE121 pKa = 3.92  FF122 pKa = 4.64  
 14.43 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.718 
IPC2_protein 3.745 
IPC_protein 3.77 
Toseland    3.528 
ProMoST     3.948 
Dawson      3.77 
Bjellqvist  3.923 
Wikipedia   3.732 
Rodwell     3.592 
Grimsley    3.439 
Solomon     3.757 
Lehninger   3.719 
Nozaki      3.884 
DTASelect   4.164 
Thurlkill   3.592 
EMBOSS      3.745 
Sillero     3.884 
Patrickios  1.888 
IPC_peptide 3.757 
IPC2_peptide  3.859 
IPC2.peptide.svr19  3.802 
 Protein with the highest isoelectric point: 
>tr|C6M575|C6M575_NEISI Uncharacterized protein OS=Neisseria sicca ATCC 29256 OX=547045 GN=NEISICOT_01675 PE=4 SV=1MM1 pKa = 7.35  KK2 pKa = 9.36  RR3 pKa = 11.84  TYY5 pKa = 10.14  QPSVTKK11 pKa = 10.56  RR12 pKa = 11.84  KK13 pKa = 7.91  RR14 pKa = 11.84  THH16 pKa = 5.89  GFLVRR21 pKa = 11.84  SKK23 pKa = 9.38  TRR25 pKa = 11.84  GGRR28 pKa = 11.84  AVLAARR34 pKa = 11.84  RR35 pKa = 11.84  AKK37 pKa = 9.87  GRR39 pKa = 11.84  KK40 pKa = 8.75  RR41 pKa = 11.84  LAVV44 pKa = 3.41  
 5.05 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.413 
IPC2_protein 11.096 
IPC_protein 12.457 
Toseland    12.618 
ProMoST     13.1 
Dawson      12.618 
Bjellqvist  12.603 
Wikipedia   13.086 
Rodwell     12.34 
Grimsley    12.661 
Solomon     13.1 
Lehninger   13.013 
Nozaki      12.618 
DTASelect   12.603 
Thurlkill   12.618 
EMBOSS      13.115 
Sillero     12.618 
Patrickios  12.076 
IPC_peptide 13.115 
IPC2_peptide  12.091 
IPC2.peptide.svr19  9.034 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        3646 
0
3646 
821177
34
3862
225.2
25.03
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        9.72 ± 0.067
1.131 ± 0.017
5.315 ± 0.041
6.041 ± 0.057
4.445 ± 0.035
7.25 ± 0.059
2.218 ± 0.024
5.777 ± 0.042
5.663 ± 0.04
9.873 ± 0.065
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.531 ± 0.025
4.229 ± 0.049
4.226 ± 0.037
4.289 ± 0.032
5.369 ± 0.049
5.699 ± 0.033
5.208 ± 0.043
6.627 ± 0.045
1.291 ± 0.021
3.099 ± 0.028
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here