Dialister sp. CAG:357
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1809 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7CLP4|R7CLP4_9FIRM DNA repair protein RadC OS=Dialister sp. CAG:357 OX=1262869 GN=BN625_01024 PE=3 SV=1
MM1 pKa = 8.4 DD2 pKa = 3.83 ITNLQSDD9 pKa = 3.66 MDD11 pKa = 4.09 YY12 pKa = 11.42 LFNCNFFDD20 pKa = 6.0 DD21 pKa = 4.71 SFDD24 pKa = 4.31 PEE26 pKa = 4.26 DD27 pKa = 5.18 HH28 pKa = 7.33 EE29 pKa = 4.8 DD30 pKa = 3.66 HH31 pKa = 7.16 QDD33 pKa = 3.36 FADD36 pKa = 4.75 KK37 pKa = 10.92 VLQSYY42 pKa = 9.79 SWDD45 pKa = 3.53 EE46 pKa = 3.57 IYY48 pKa = 10.86 GYY50 pKa = 9.04 FFRR53 pKa = 11.84 HH54 pKa = 5.66 LTQDD58 pKa = 3.26 CTTAEE63 pKa = 4.15 SVYY66 pKa = 10.92 NATNLYY72 pKa = 9.91 FVYY75 pKa = 10.38 CFDD78 pKa = 4.9 KK79 pKa = 11.34 NPVPNPYY86 pKa = 10.32 EE87 pKa = 3.63 LVGYY91 pKa = 9.1 VLFRR95 pKa = 11.84 IDD97 pKa = 4.54 LDD99 pKa = 4.23 KK100 pKa = 11.68 YY101 pKa = 7.54 WDD103 pKa = 3.79 EE104 pKa = 4.48 YY105 pKa = 11.74 GDD107 pKa = 4.58 FIDD110 pKa = 4.52 SFAVSVLEE118 pKa = 4.01 SSKK121 pKa = 10.71 KK122 pKa = 9.63 VSLMTDD128 pKa = 3.66 PYY130 pKa = 9.1 YY131 pKa = 11.13 APWEE135 pKa = 4.17 DD136 pKa = 3.34 PKK138 pKa = 11.25 VIKK141 pKa = 10.63 QMEE144 pKa = 4.16 KK145 pKa = 9.72 WKK147 pKa = 10.56 KK148 pKa = 8.65 QLQNNEE154 pKa = 3.53 SDD156 pKa = 3.27
Molecular weight: 18.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.834
IPC2_protein 4.012
IPC_protein 4.012
Toseland 3.795
ProMoST 4.126
Dawson 3.999
Bjellqvist 4.19
Wikipedia 3.948
Rodwell 3.834
Grimsley 3.694
Solomon 3.999
Lehninger 3.961
Nozaki 4.113
DTASelect 4.368
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.126
Patrickios 0.731
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.036
Protein with the highest isoelectric point:
>tr|R7CRX6|R7CRX6_9FIRM CitMHS domain-containing protein OS=Dialister sp. CAG:357 OX=1262869 GN=BN625_00197 PE=3 SV=1
MM1 pKa = 7.7 AKK3 pKa = 7.99 MTFQPNNHH11 pKa = 5.63 WRR13 pKa = 11.84 KK14 pKa = 6.87 QTHH17 pKa = 5.61 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MKK24 pKa = 8.64 TKK26 pKa = 10.33 AGRR29 pKa = 11.84 IVLKK33 pKa = 10.36 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.89 GRR40 pKa = 11.84 KK41 pKa = 8.67 VLSAA45 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1809
0
1809
544003
30
2013
300.7
33.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.626 ± 0.068
1.189 ± 0.021
5.888 ± 0.059
6.963 ± 0.052
4.16 ± 0.044
7.732 ± 0.069
2.145 ± 0.032
7.04 ± 0.043
6.526 ± 0.056
9.058 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.134 ± 0.033
3.702 ± 0.04
3.907 ± 0.04
2.749 ± 0.035
4.782 ± 0.055
5.829 ± 0.057
5.261 ± 0.055
6.859 ± 0.046
0.967 ± 0.022
3.48 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here