Amino acid dipepetide frequency for Garciella nitratireducens DSM 15102

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.036AlaAla: 4.036 ± 0.11
0.688AlaCys: 0.688 ± 0.036
2.442AlaAsp: 2.442 ± 0.075
3.074AlaGlu: 3.074 ± 0.081
2.596AlaPhe: 2.596 ± 0.067
4.131AlaGly: 4.131 ± 0.089
1.045AlaHis: 1.045 ± 0.042
6.21AlaIle: 6.21 ± 0.11
4.972AlaLys: 4.972 ± 0.099
5.739AlaLeu: 5.739 ± 0.083
1.674AlaMet: 1.674 ± 0.054
2.413AlaAsn: 2.413 ± 0.068
1.449AlaPro: 1.449 ± 0.051
2.284AlaGln: 2.284 ± 0.066
2.04AlaArg: 2.04 ± 0.057
3.107AlaSer: 3.107 ± 0.077
2.876AlaThr: 2.876 ± 0.071
3.907AlaVal: 3.907 ± 0.096
0.35AlaTrp: 0.35 ± 0.026
2.043AlaTyr: 2.043 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
0.549CysAla: 0.549 ± 0.029
0.135CysCys: 0.135 ± 0.013
0.457CysAsp: 0.457 ± 0.028
0.607CysGlu: 0.607 ± 0.037
0.444CysPhe: 0.444 ± 0.025
0.911CysGly: 0.911 ± 0.045
0.221CysHis: 0.221 ± 0.023
1.048CysIle: 1.048 ± 0.038
0.772CysLys: 0.772 ± 0.038
0.784CysLeu: 0.784 ± 0.032
0.229CysMet: 0.229 ± 0.019
0.543CysAsn: 0.543 ± 0.029
0.526CysPro: 0.526 ± 0.033
0.306CysGln: 0.306 ± 0.021
0.344CysArg: 0.344 ± 0.024
0.664CysSer: 0.664 ± 0.037
0.499CysThr: 0.499 ± 0.027
0.503CysVal: 0.503 ± 0.029
0.065CysTrp: 0.065 ± 0.01
0.36CysTyr: 0.36 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
2.574AspAla: 2.574 ± 0.073
0.57AspCys: 0.57 ± 0.031
1.652AspAsp: 1.652 ± 0.052
3.268AspGlu: 3.268 ± 0.084
2.731AspPhe: 2.731 ± 0.066
2.837AspGly: 2.837 ± 0.071
0.927AspHis: 0.927 ± 0.038
5.625AspIle: 5.625 ± 0.091
4.053AspLys: 4.053 ± 0.09
5.148AspLeu: 5.148 ± 0.069
1.32AspMet: 1.32 ± 0.044
1.846AspAsn: 1.846 ± 0.051
1.806AspPro: 1.806 ± 0.054
1.814AspGln: 1.814 ± 0.058
1.854AspArg: 1.854 ± 0.06
2.475AspSer: 2.475 ± 0.063
2.373AspThr: 2.373 ± 0.068
3.047AspVal: 3.047 ± 0.074
0.408AspTrp: 0.408 ± 0.026
2.185AspTyr: 2.185 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
3.826GluAla: 3.826 ± 0.089
0.575GluCys: 0.575 ± 0.031
4.077GluAsp: 4.077 ± 0.077
7.728GluGlu: 7.728 ± 0.14
2.853GluPhe: 2.853 ± 0.074
4.305GluGly: 4.305 ± 0.096
1.019GluHis: 1.019 ± 0.042
8.279GluIle: 8.279 ± 0.13
9.391GluLys: 9.391 ± 0.15
6.219GluLeu: 6.219 ± 0.107
2.278GluMet: 2.278 ± 0.067
5.041GluAsn: 5.041 ± 0.092
1.481GluPro: 1.481 ± 0.055
2.83GluGln: 2.83 ± 0.07
2.87GluArg: 2.87 ± 0.078
3.09GluSer: 3.09 ± 0.071
2.754GluThr: 2.754 ± 0.072
4.702GluVal: 4.702 ± 0.087
0.422GluTrp: 0.422 ± 0.025
2.975GluTyr: 2.975 ± 0.077
0.0GluXaa: 0.0 ± 0.0
Phe
2.244PheAla: 2.244 ± 0.068
0.465PheCys: 0.465 ± 0.026
2.192PheAsp: 2.192 ± 0.06
2.475PheGlu: 2.475 ± 0.066
2.295PhePhe: 2.295 ± 0.072
2.902PheGly: 2.902 ± 0.08
0.973PheHis: 0.973 ± 0.04
4.899PheIle: 4.899 ± 0.112
2.939PheLys: 2.939 ± 0.066
4.546PheLeu: 4.546 ± 0.105
1.088PheMet: 1.088 ± 0.045
1.919PheAsn: 1.919 ± 0.06
1.58PhePro: 1.58 ± 0.058
1.679PheGln: 1.679 ± 0.05
1.269PheArg: 1.269 ± 0.046
3.034PheSer: 3.034 ± 0.072
2.289PheThr: 2.289 ± 0.07
2.387PheVal: 2.387 ± 0.06
0.306PheTrp: 0.306 ± 0.021
1.894PheTyr: 1.894 ± 0.067
0.0PheXaa: 0.0 ± 0.0
Gly
4.377GlyAla: 4.377 ± 0.115
0.811GlyCys: 0.811 ± 0.04
2.997GlyAsp: 2.997 ± 0.072
4.445GlyGlu: 4.445 ± 0.099
2.954GlyPhe: 2.954 ± 0.075
4.753GlyGly: 4.753 ± 0.103
1.059GlyHis: 1.059 ± 0.04
7.57GlyIle: 7.57 ± 0.133
6.121GlyLys: 6.121 ± 0.101
5.844GlyLeu: 5.844 ± 0.104
2.061GlyMet: 2.061 ± 0.064
3.063GlyAsn: 3.063 ± 0.071
1.543GlyPro: 1.543 ± 0.053
2.012GlyGln: 2.012 ± 0.064
2.458GlyArg: 2.458 ± 0.071
3.453GlySer: 3.453 ± 0.074
3.429GlyThr: 3.429 ± 0.073
4.638GlyVal: 4.638 ± 0.09
0.511GlyTrp: 0.511 ± 0.031
2.833GlyTyr: 2.833 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
0.862HisAla: 0.862 ± 0.037
0.253HisCys: 0.253 ± 0.021
0.639HisAsp: 0.639 ± 0.029
0.954HisGlu: 0.954 ± 0.04
0.86HisPhe: 0.86 ± 0.038
1.183HisGly: 1.183 ± 0.051
0.427HisHis: 0.427 ± 0.028
1.682HisIle: 1.682 ± 0.05
1.23HisLys: 1.23 ± 0.05
1.701HisLeu: 1.701 ± 0.057
0.43HisMet: 0.43 ± 0.025
0.784HisAsn: 0.784 ± 0.039
0.984HisPro: 0.984 ± 0.036
0.696HisGln: 0.696 ± 0.035
0.714HisArg: 0.714 ± 0.035
1.018HisSer: 1.018 ± 0.041
0.889HisThr: 0.889 ± 0.035
0.851HisVal: 0.851 ± 0.038
0.135HisTrp: 0.135 ± 0.016
0.793HisTyr: 0.793 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.516IleAla: 6.516 ± 0.125
1.062IleCys: 1.062 ± 0.049
5.732IleAsp: 5.732 ± 0.096
8.147IleGlu: 8.147 ± 0.12
4.695IlePhe: 4.695 ± 0.125
7.201IleGly: 7.201 ± 0.147
1.94IleHis: 1.94 ± 0.052
10.067IleIle: 10.067 ± 0.18
8.142IleLys: 8.142 ± 0.134
10.579IleLeu: 10.579 ± 0.163
2.365IleMet: 2.365 ± 0.063
4.595IleAsn: 4.595 ± 0.08
4.093IlePro: 4.093 ± 0.077
4.189IleGln: 4.189 ± 0.09
3.596IleArg: 3.596 ± 0.07
6.57IleSer: 6.57 ± 0.107
4.898IleThr: 4.898 ± 0.109
6.159IleVal: 6.159 ± 0.109
0.596IleTrp: 0.596 ± 0.03
3.73IleTyr: 3.73 ± 0.09
0.0IleXaa: 0.0 ± 0.0
Lys
4.509LysAla: 4.509 ± 0.094
0.664LysCys: 0.664 ± 0.036
5.085LysAsp: 5.085 ± 0.098
10.138LysGlu: 10.138 ± 0.13
2.531LysPhe: 2.531 ± 0.068
5.401LysGly: 5.401 ± 0.104
1.101LysHis: 1.101 ± 0.042
9.712LysIle: 9.712 ± 0.116
10.461LysLys: 10.461 ± 0.148
6.635LysLeu: 6.635 ± 0.11
2.622LysMet: 2.622 ± 0.058
6.272LysAsn: 6.272 ± 0.114
2.099LysPro: 2.099 ± 0.057
2.997LysGln: 2.997 ± 0.088
3.545LysArg: 3.545 ± 0.088
4.109LysSer: 4.109 ± 0.083
3.746LysThr: 3.746 ± 0.079
5.503LysVal: 5.503 ± 0.093
0.642LysTrp: 0.642 ± 0.032
3.472LysTyr: 3.472 ± 0.076
0.0LysXaa: 0.0 ± 0.0
Leu
5.453LeuAla: 5.453 ± 0.104
0.858LeuCys: 0.858 ± 0.044
4.738LeuAsp: 4.738 ± 0.094
7.645LeuGlu: 7.645 ± 0.122
4.076LeuPhe: 4.076 ± 0.11
6.624LeuGly: 6.624 ± 0.111
1.339LeuHis: 1.339 ± 0.042
8.174LeuIle: 8.174 ± 0.128
8.819LeuLys: 8.819 ± 0.119
8.827LeuLeu: 8.827 ± 0.156
2.321LeuMet: 2.321 ± 0.065
4.877LeuAsn: 4.877 ± 0.1
3.275LeuPro: 3.275 ± 0.076
3.131LeuGln: 3.131 ± 0.073
3.174LeuArg: 3.174 ± 0.072
6.275LeuSer: 6.275 ± 0.124
4.237LeuThr: 4.237 ± 0.077
5.207LeuVal: 5.207 ± 0.078
0.628LeuTrp: 0.628 ± 0.031
3.068LeuTyr: 3.068 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
1.695MetAla: 1.695 ± 0.053
0.178MetCys: 0.178 ± 0.017
1.642MetAsp: 1.642 ± 0.046
2.25MetGlu: 2.25 ± 0.059
0.752MetPhe: 0.752 ± 0.033
2.053MetGly: 2.053 ± 0.056
0.36MetHis: 0.36 ± 0.024
2.848MetIle: 2.848 ± 0.078
2.666MetLys: 2.666 ± 0.064
2.147MetLeu: 2.147 ± 0.066
0.721MetMet: 0.721 ± 0.036
1.569MetAsn: 1.569 ± 0.047
0.811MetPro: 0.811 ± 0.036
0.878MetGln: 0.878 ± 0.034
0.9MetArg: 0.9 ± 0.041
1.468MetSer: 1.468 ± 0.048
1.191MetThr: 1.191 ± 0.042
1.777MetVal: 1.777 ± 0.057
0.119MetTrp: 0.119 ± 0.013
0.769MetTyr: 0.769 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.767AsnAla: 2.767 ± 0.07
0.538AsnCys: 0.538 ± 0.028
1.926AsnAsp: 1.926 ± 0.056
3.15AsnGlu: 3.15 ± 0.072
2.255AsnPhe: 2.255 ± 0.064
2.897AsnGly: 2.897 ± 0.071
1.12AsnHis: 1.12 ± 0.043
6.161AsnIle: 6.161 ± 0.11
4.676AsnLys: 4.676 ± 0.095
4.998AsnLeu: 4.998 ± 0.093
1.376AsnMet: 1.376 ± 0.048
2.571AsnAsn: 2.571 ± 0.067
2.19AsnPro: 2.19 ± 0.053
2.287AsnGln: 2.287 ± 0.061
2.016AsnArg: 2.016 ± 0.056
2.665AsnSer: 2.665 ± 0.075
2.491AsnThr: 2.491 ± 0.068
2.636AsnVal: 2.636 ± 0.076
0.42AsnTrp: 0.42 ± 0.03
2.053AsnTyr: 2.053 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
1.569ProAla: 1.569 ± 0.058
0.349ProCys: 0.349 ± 0.023
1.486ProAsp: 1.486 ± 0.053
2.569ProGlu: 2.569 ± 0.081
1.661ProPhe: 1.661 ± 0.055
2.193ProGly: 2.193 ± 0.061
0.618ProHis: 0.618 ± 0.033
3.585ProIle: 3.585 ± 0.08
2.615ProLys: 2.615 ± 0.068
2.881ProLeu: 2.881 ± 0.081
0.827ProMet: 0.827 ± 0.038
1.642ProAsn: 1.642 ± 0.057
0.887ProPro: 0.887 ± 0.038
1.073ProGln: 1.073 ± 0.04
0.967ProArg: 0.967 ± 0.043
1.867ProSer: 1.867 ± 0.058
1.695ProThr: 1.695 ± 0.06
2.254ProVal: 2.254 ± 0.066
0.248ProTrp: 0.248 ± 0.021
1.445ProTyr: 1.445 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
2.185GlnAla: 2.185 ± 0.068
0.298GlnCys: 0.298 ± 0.024
1.658GlnAsp: 1.658 ± 0.053
3.485GlnGlu: 3.485 ± 0.08
1.263GlnPhe: 1.263 ± 0.045
2.348GlnGly: 2.348 ± 0.064
0.518GlnHis: 0.518 ± 0.03
3.295GlnIle: 3.295 ± 0.078
3.907GlnLys: 3.907 ± 0.091
3.341GlnLeu: 3.341 ± 0.082
1.003GlnMet: 1.003 ± 0.045
1.964GlnAsn: 1.964 ± 0.053
0.989GlnPro: 0.989 ± 0.045
1.685GlnGln: 1.685 ± 0.069
1.519GlnArg: 1.519 ± 0.054
1.906GlnSer: 1.906 ± 0.065
1.413GlnThr: 1.413 ± 0.044
2.134GlnVal: 2.134 ± 0.066
0.331GlnTrp: 0.331 ± 0.023
1.387GlnTyr: 1.387 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
1.986ArgAla: 1.986 ± 0.063
0.363ArgCys: 0.363 ± 0.027
1.806ArgAsp: 1.806 ± 0.055
3.16ArgGlu: 3.16 ± 0.07
1.424ArgPhe: 1.424 ± 0.049
2.193ArgGly: 2.193 ± 0.062
0.529ArgHis: 0.529 ± 0.035
3.593ArgIle: 3.593 ± 0.075
3.693ArgLys: 3.693 ± 0.082
3.045ArgLeu: 3.045 ± 0.08
1.113ArgMet: 1.113 ± 0.049
1.951ArgAsn: 1.951 ± 0.057
1.128ArgPro: 1.128 ± 0.046
1.234ArgGln: 1.234 ± 0.044
1.548ArgArg: 1.548 ± 0.054
1.728ArgSer: 1.728 ± 0.053
1.559ArgThr: 1.559 ± 0.053
2.33ArgVal: 2.33 ± 0.064
0.263ArgTrp: 0.263 ± 0.023
1.405ArgTyr: 1.405 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
2.915SerAla: 2.915 ± 0.066
0.602SerCys: 0.602 ± 0.032
2.352SerAsp: 2.352 ± 0.068
3.138SerGlu: 3.138 ± 0.068
2.919SerPhe: 2.919 ± 0.082
3.888SerGly: 3.888 ± 0.087
0.997SerHis: 0.997 ± 0.042
6.468SerIle: 6.468 ± 0.114
4.847SerLys: 4.847 ± 0.089
5.527SerLeu: 5.527 ± 0.103
1.629SerMet: 1.629 ± 0.049
2.846SerAsn: 2.846 ± 0.072
1.844SerPro: 1.844 ± 0.054
2.024SerGln: 2.024 ± 0.055
1.962SerArg: 1.962 ± 0.055
3.475SerSer: 3.475 ± 0.082
2.894SerThr: 2.894 ± 0.08
3.021SerVal: 3.021 ± 0.073
0.374SerTrp: 0.374 ± 0.025
2.171SerTyr: 2.171 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
2.935ThrAla: 2.935 ± 0.073
0.385ThrCys: 0.385 ± 0.023
2.002ThrAsp: 2.002 ± 0.055
2.762ThrGlu: 2.762 ± 0.071
2.086ThrPhe: 2.086 ± 0.056
3.784ThrGly: 3.784 ± 0.09
0.914ThrHis: 0.914 ± 0.038
5.117ThrIle: 5.117 ± 0.105
3.463ThrLys: 3.463 ± 0.066
4.67ThrLeu: 4.67 ± 0.086
1.12ThrMet: 1.12 ± 0.046
2.147ThrAsn: 2.147 ± 0.064
2.02ThrPro: 2.02 ± 0.058
1.628ThrGln: 1.628 ± 0.055
1.564ThrArg: 1.564 ± 0.046
2.776ThrSer: 2.776 ± 0.061
2.579ThrThr: 2.579 ± 0.07
2.76ThrVal: 2.76 ± 0.061
0.304ThrTrp: 0.304 ± 0.023
1.774ThrTyr: 1.774 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
3.752ValAla: 3.752 ± 0.091
0.631ValCys: 0.631 ± 0.034
3.278ValAsp: 3.278 ± 0.076
4.635ValGlu: 4.635 ± 0.097
2.652ValPhe: 2.652 ± 0.072
4.286ValGly: 4.286 ± 0.088
0.895ValHis: 0.895 ± 0.037
6.046ValIle: 6.046 ± 0.094
4.816ValLys: 4.816 ± 0.108
5.783ValLeu: 5.783 ± 0.1
1.523ValMet: 1.523 ± 0.055
2.872ValAsn: 2.872 ± 0.065
2.126ValPro: 2.126 ± 0.06
1.851ValGln: 1.851 ± 0.054
1.961ValArg: 1.961 ± 0.074
3.7ValSer: 3.7 ± 0.071
2.859ValThr: 2.859 ± 0.061
4.229ValVal: 4.229 ± 0.093
0.339ValTrp: 0.339 ± 0.024
2.102ValTyr: 2.102 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.385TrpAla: 0.385 ± 0.029
0.072TrpCys: 0.072 ± 0.01
0.333TrpAsp: 0.333 ± 0.028
0.535TrpGlu: 0.535 ± 0.03
0.279TrpPhe: 0.279 ± 0.025
0.454TrpGly: 0.454 ± 0.032
0.116TrpHis: 0.116 ± 0.013
0.772TrpIle: 0.772 ± 0.035
0.637TrpLys: 0.637 ± 0.031
0.642TrpLeu: 0.642 ± 0.031
0.228TrpMet: 0.228 ± 0.018
0.427TrpAsn: 0.427 ± 0.03
0.167TrpPro: 0.167 ± 0.02
0.183TrpGln: 0.183 ± 0.019
0.239TrpArg: 0.239 ± 0.017
0.323TrpSer: 0.323 ± 0.024
0.336TrpThr: 0.336 ± 0.025
0.349TrpVal: 0.349 ± 0.023
0.068TrpTrp: 0.068 ± 0.01
0.277TrpTyr: 0.277 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.943TyrAla: 1.943 ± 0.053
0.47TyrCys: 0.47 ± 0.027
2.02TyrAsp: 2.02 ± 0.056
2.55TyrGlu: 2.55 ± 0.063
2.047TyrPhe: 2.047 ± 0.058
2.588TyrGly: 2.588 ± 0.064
0.965TyrHis: 0.965 ± 0.04
3.721TyrIle: 3.721 ± 0.086
3.025TyrLys: 3.025 ± 0.083
3.69TyrLeu: 3.69 ± 0.079
0.866TyrMet: 0.866 ± 0.038
1.852TyrAsn: 1.852 ± 0.055
1.432TyrPro: 1.432 ± 0.049
1.849TyrGln: 1.849 ± 0.057
1.511TyrArg: 1.511 ± 0.053
2.19TyrSer: 2.19 ± 0.055
1.785TyrThr: 1.785 ± 0.048
1.942TyrVal: 1.942 ± 0.059
0.291TyrTrp: 0.291 ± 0.023
1.795TyrTyr: 1.795 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2103 proteins (627865 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski