Ctenophore-associated circular virus 3
Average proteome isoelectric point is 8.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141MJB0|A0A141MJB0_9VIRU Uncharacterized protein OS=Ctenophore-associated circular virus 3 OX=1778560 PE=4 SV=1
MM1 pKa = 7.91 PGYY4 pKa = 9.29 QFRR7 pKa = 11.84 SATFTQFNVEE17 pKa = 4.18 KK18 pKa = 10.62 APEE21 pKa = 4.47 LGNLQYY27 pKa = 10.78 IAFGLEE33 pKa = 4.31 TCPSTQRR40 pKa = 11.84 QHH42 pKa = 4.93 WQGFAYY48 pKa = 10.21 SYY50 pKa = 11.3 TKK52 pKa = 8.21 MTHH55 pKa = 5.52 KK56 pKa = 10.1 KK57 pKa = 8.59 WRR59 pKa = 11.84 EE60 pKa = 3.95 TFPGAHH66 pKa = 5.97 IEE68 pKa = 4.06 EE69 pKa = 4.45 MKK71 pKa = 11.19 GNFRR75 pKa = 11.84 QNEE78 pKa = 4.69 VYY80 pKa = 10.2 CSKK83 pKa = 10.7 EE84 pKa = 3.51 GTYY87 pKa = 10.1 TEE89 pKa = 5.02 FGIKK93 pKa = 10.02 PMEE96 pKa = 4.42 NGKK99 pKa = 9.73 KK100 pKa = 9.94 RR101 pKa = 11.84 SLAEE105 pKa = 3.87 MKK107 pKa = 10.23 EE108 pKa = 3.86 QLDD111 pKa = 3.71 NGEE114 pKa = 4.48 KK115 pKa = 9.72 PVEE118 pKa = 4.13 VATKK122 pKa = 9.07 SDD124 pKa = 3.15 KK125 pKa = 10.88 MFGTFLQYY133 pKa = 10.62 RR134 pKa = 11.84 SGLNEE139 pKa = 3.42 YY140 pKa = 9.88 AQYY143 pKa = 10.58 IRR145 pKa = 11.84 EE146 pKa = 4.34 KK147 pKa = 10.45 KK148 pKa = 10.42 LKK150 pKa = 10.42 LNRR153 pKa = 11.84 DD154 pKa = 3.28 VPDD157 pKa = 3.45 VYY159 pKa = 11.46 VRR161 pKa = 11.84 IGPSGTGKK169 pKa = 8.13 TRR171 pKa = 11.84 WLDD174 pKa = 3.34 DD175 pKa = 3.52 TFGLDD180 pKa = 3.13 GWTFAPDD187 pKa = 3.65 NTGHH191 pKa = 6.86 WFDD194 pKa = 5.0 GCDD197 pKa = 3.54 CDD199 pKa = 5.59 VICFDD204 pKa = 4.2 EE205 pKa = 4.91 VEE207 pKa = 4.41 AGAIPSLSTWKK218 pKa = 10.16 ILCDD222 pKa = 3.58 RR223 pKa = 11.84 YY224 pKa = 10.82 GKK226 pKa = 10.1 RR227 pKa = 11.84 VPVKK231 pKa = 10.6 GGFIYY236 pKa = 9.77 WKK238 pKa = 9.17 PKK240 pKa = 10.38 VIVFTSNQPISKK252 pKa = 8.42 WWPNLTEE259 pKa = 4.19 FDD261 pKa = 4.73 LIAIEE266 pKa = 4.37 RR267 pKa = 11.84 RR268 pKa = 11.84 CKK270 pKa = 10.4 EE271 pKa = 3.84 IVVVEE276 pKa = 4.1
Molecular weight: 31.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.825
IPC2_protein 7.146
IPC_protein 7.117
Toseland 6.766
ProMoST 7.497
Dawson 7.805
Bjellqvist 8.053
Wikipedia 7.717
Rodwell 7.805
Grimsley 6.795
Solomon 7.878
Lehninger 7.892
Nozaki 8.302
DTASelect 7.907
Thurlkill 7.966
EMBOSS 8.024
Sillero 8.273
Patrickios 4.266
IPC_peptide 7.878
IPC2_peptide 7.19
IPC2.peptide.svr19 7.375
Protein with the highest isoelectric point:
>tr|A0A141MJB0|A0A141MJB0_9VIRU Uncharacterized protein OS=Ctenophore-associated circular virus 3 OX=1778560 PE=4 SV=1
MM1 pKa = 7.91 PIGRR5 pKa = 11.84 KK6 pKa = 8.77 RR7 pKa = 11.84 KK8 pKa = 9.38 SYY10 pKa = 9.44 ATAPYY15 pKa = 9.36 IKK17 pKa = 9.8 RR18 pKa = 11.84 RR19 pKa = 11.84 KK20 pKa = 6.98 TPYY23 pKa = 8.92 RR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 10.2 SFMSRR31 pKa = 11.84 NVSRR35 pKa = 11.84 IARR38 pKa = 11.84 QVVMRR43 pKa = 11.84 AAEE46 pKa = 4.23 TKK48 pKa = 10.11 TKK50 pKa = 10.15 VINYY54 pKa = 7.65 GKK56 pKa = 8.74 TEE58 pKa = 4.51 LYY60 pKa = 10.59 HH61 pKa = 6.52 NSLSVTHH68 pKa = 6.75 KK69 pKa = 11.14 LNFTAHH75 pKa = 6.06 MPAQGDD81 pKa = 4.0 GKK83 pKa = 10.27 DD84 pKa = 3.68 GRR86 pKa = 11.84 SGDD89 pKa = 4.1 SIIGKK94 pKa = 8.21 GWKK97 pKa = 9.9 LRR99 pKa = 11.84 MTLANKK105 pKa = 9.97 GDD107 pKa = 4.06 RR108 pKa = 11.84 PNVTYY113 pKa = 10.77 RR114 pKa = 11.84 LVVFAAKK121 pKa = 10.42 AGVTPTYY128 pKa = 10.95 SNTFVNVTGNGLLDD142 pKa = 4.99 GINTDD147 pKa = 3.19 RR148 pKa = 11.84 CTILYY153 pKa = 8.57 QKK155 pKa = 8.7 WLKK158 pKa = 9.25 PNKK161 pKa = 10.05 GMVGTSEE168 pKa = 4.02 TGVRR172 pKa = 11.84 EE173 pKa = 4.07 YY174 pKa = 11.57 VSTKK178 pKa = 10.45 SIWIKK183 pKa = 10.2 RR184 pKa = 11.84 EE185 pKa = 3.41 KK186 pKa = 10.58 VMKK189 pKa = 10.7 FNGLEE194 pKa = 4.01 VNDD197 pKa = 4.03 RR198 pKa = 11.84 DD199 pKa = 4.92 VYY201 pKa = 11.13 FCMLAYY207 pKa = 10.1 DD208 pKa = 4.31 AYY210 pKa = 10.22 GTLTADD216 pKa = 3.86 NIGYY220 pKa = 7.81 MQMWGEE226 pKa = 4.45 FKK228 pKa = 11.22 YY229 pKa = 10.67 KK230 pKa = 10.71 DD231 pKa = 3.43 PP232 pKa = 4.96
Molecular weight: 26.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.218
IPC2_protein 9.619
IPC_protein 9.736
Toseland 10.218
ProMoST 9.882
Dawson 10.409
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.877
Grimsley 10.467
Solomon 10.423
Lehninger 10.394
Nozaki 10.204
DTASelect 10.058
Thurlkill 10.262
EMBOSS 10.613
Sillero 10.306
Patrickios 10.496
IPC_peptide 10.423
IPC2_peptide 8.726
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
508
232
276
254.0
29.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.118 ± 0.61
1.772 ± 0.605
4.921 ± 0.12
5.709 ± 1.791
4.724 ± 1.136
8.465 ± 0.104
1.378 ± 0.056
4.921 ± 0.12
9.252 ± 0.441
5.512 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.15 ± 0.773
4.921 ± 0.741
4.331 ± 0.587
2.953 ± 0.818
6.299 ± 0.971
4.724 ± 0.298
7.677 ± 0.628
6.496 ± 0.553
2.559 ± 0.556
5.118 ± 0.61
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here