Salmonella phage NR01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus; Salmonella virus NR01

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A162EAR5|A0A162EAR5_9CAUD Uncharacterized protein OS=Salmonella phage NR01 OX=1647411 GN=NR01_0115 PE=4 SV=1
MM1 pKa = 6.89QVYY4 pKa = 10.36VLTRR8 pKa = 11.84DD9 pKa = 3.25INEE12 pKa = 3.91YY13 pKa = 9.95NQDD16 pKa = 2.51GEE18 pKa = 4.74YY19 pKa = 9.78FVKK22 pKa = 10.56VFAEE26 pKa = 4.49KK27 pKa = 10.07PSKK30 pKa = 10.21QQLLDD35 pKa = 3.63AGVPEE40 pKa = 4.86DD41 pKa = 3.5QAKK44 pKa = 10.79CILQDD49 pKa = 3.91KK50 pKa = 9.66EE51 pKa = 4.39FTGDD55 pKa = 3.25AYY57 pKa = 10.56EE58 pKa = 4.91CFYY61 pKa = 11.16LSCEE65 pKa = 4.28NII67 pKa = 3.48

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161BZZ4|A0A161BZZ4_9CAUD Uncharacterized protein OS=Salmonella phage NR01 OX=1647411 GN=NR01_0110 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.53KK3 pKa = 10.26LLAYY7 pKa = 8.75LLCFVVFARR16 pKa = 11.84PFLLTLMRR24 pKa = 11.84RR25 pKa = 11.84AQVAAWCPVALVFTFLYY42 pKa = 9.15KK43 pKa = 10.85NKK45 pKa = 9.63VAAFSLFVIIYY56 pKa = 9.81LVGRR60 pKa = 11.84QQ61 pKa = 3.19

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

148

0

148

32468

50

1226

219.4

24.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.546 ± 0.43

1.14 ± 0.097

5.926 ± 0.157

6.822 ± 0.163

4.038 ± 0.152

6.591 ± 0.235

1.866 ± 0.139

6.616 ± 0.183

7.296 ± 0.196

8.593 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.138

5.633 ± 0.162

3.456 ± 0.124

3.745 ± 0.19

4.635 ± 0.144

6.419 ± 0.173

5.726 ± 0.184

6.209 ± 0.136

1.272 ± 0.093

4.044 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski