Amino acid dipepetide frequency for Escherichia phage OSYSP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.904AlaAla: 7.904 ± 1.202
0.677AlaCys: 0.677 ± 0.151
4.097AlaAsp: 4.097 ± 0.375
5.549AlaGlu: 5.549 ± 0.457
3.162AlaPhe: 3.162 ± 0.27
5.452AlaGly: 5.452 ± 0.496
1.355AlaHis: 1.355 ± 0.189
5.226AlaIle: 5.226 ± 0.44
6.775AlaLys: 6.775 ± 0.615
6.323AlaLeu: 6.323 ± 0.533
1.936AlaMet: 1.936 ± 0.295
3.387AlaAsn: 3.387 ± 0.303
2.258AlaPro: 2.258 ± 0.285
3.42AlaGln: 3.42 ± 0.357
3.033AlaArg: 3.033 ± 0.335
5.033AlaSer: 5.033 ± 0.719
3.742AlaThr: 3.742 ± 0.429
4.678AlaVal: 4.678 ± 0.437
0.839AlaTrp: 0.839 ± 0.163
2.71AlaTyr: 2.71 ± 0.265
0.0AlaXaa: 0.0 ± 0.0
Cys
0.71CysAla: 0.71 ± 0.155
0.226CysCys: 0.226 ± 0.105
0.516CysAsp: 0.516 ± 0.121
0.839CysGlu: 0.839 ± 0.181
0.548CysPhe: 0.548 ± 0.148
0.839CysGly: 0.839 ± 0.169
0.29CysHis: 0.29 ± 0.094
0.71CysIle: 0.71 ± 0.135
0.807CysLys: 0.807 ± 0.196
0.774CysLeu: 0.774 ± 0.143
0.387CysMet: 0.387 ± 0.15
0.645CysAsn: 0.645 ± 0.127
0.419CysPro: 0.419 ± 0.125
0.355CysGln: 0.355 ± 0.116
0.613CysArg: 0.613 ± 0.139
0.807CysSer: 0.807 ± 0.147
0.581CysThr: 0.581 ± 0.125
0.581CysVal: 0.581 ± 0.128
0.161CysTrp: 0.161 ± 0.075
0.387CysTyr: 0.387 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
4.71AspAla: 4.71 ± 0.348
0.742AspCys: 0.742 ± 0.189
3.162AspAsp: 3.162 ± 0.411
4.678AspGlu: 4.678 ± 0.376
2.71AspPhe: 2.71 ± 0.337
3.936AspGly: 3.936 ± 0.389
1.226AspHis: 1.226 ± 0.188
4.581AspIle: 4.581 ± 0.399
4.71AspLys: 4.71 ± 0.394
5.452AspLeu: 5.452 ± 0.529
1.839AspMet: 1.839 ± 0.225
2.742AspAsn: 2.742 ± 0.287
2.549AspPro: 2.549 ± 0.214
1.581AspGln: 1.581 ± 0.234
2.549AspArg: 2.549 ± 0.275
3.549AspSer: 3.549 ± 0.347
3.71AspThr: 3.71 ± 0.353
3.613AspVal: 3.613 ± 0.383
0.807AspTrp: 0.807 ± 0.172
2.645AspTyr: 2.645 ± 0.307
0.0AspXaa: 0.0 ± 0.0
Glu
5.839GluAla: 5.839 ± 0.4
0.645GluCys: 0.645 ± 0.154
3.968GluAsp: 3.968 ± 0.344
5.097GluGlu: 5.097 ± 0.473
2.549GluPhe: 2.549 ± 0.312
4.097GluGly: 4.097 ± 0.324
1.258GluHis: 1.258 ± 0.188
4.775GluIle: 4.775 ± 0.348
5.0GluLys: 5.0 ± 0.416
7.452GluLeu: 7.452 ± 0.469
2.742GluMet: 2.742 ± 0.34
2.42GluAsn: 2.42 ± 0.335
1.613GluPro: 1.613 ± 0.287
3.226GluGln: 3.226 ± 0.389
3.0GluArg: 3.0 ± 0.315
3.42GluSer: 3.42 ± 0.298
4.291GluThr: 4.291 ± 0.543
4.323GluVal: 4.323 ± 0.377
1.258GluTrp: 1.258 ± 0.208
2.807GluTyr: 2.807 ± 0.269
0.0GluXaa: 0.0 ± 0.0
Phe
2.71PheAla: 2.71 ± 0.304
0.419PheCys: 0.419 ± 0.133
2.839PheAsp: 2.839 ± 0.317
2.807PheGlu: 2.807 ± 0.36
1.387PhePhe: 1.387 ± 0.211
3.033PheGly: 3.033 ± 0.302
1.065PheHis: 1.065 ± 0.194
2.613PheIle: 2.613 ± 0.328
2.807PheLys: 2.807 ± 0.299
3.323PheLeu: 3.323 ± 0.257
0.645PheMet: 0.645 ± 0.121
2.71PheAsn: 2.71 ± 0.269
1.774PhePro: 1.774 ± 0.242
1.161PheGln: 1.161 ± 0.189
1.968PheArg: 1.968 ± 0.266
2.968PheSer: 2.968 ± 0.283
2.226PheThr: 2.226 ± 0.268
2.839PheVal: 2.839 ± 0.251
0.516PheTrp: 0.516 ± 0.148
1.645PheTyr: 1.645 ± 0.186
0.0PheXaa: 0.0 ± 0.0
Gly
4.678GlyAla: 4.678 ± 0.473
0.968GlyCys: 0.968 ± 0.191
3.839GlyAsp: 3.839 ± 0.392
4.775GlyGlu: 4.775 ± 0.362
3.162GlyPhe: 3.162 ± 0.3
3.355GlyGly: 3.355 ± 0.371
1.097GlyHis: 1.097 ± 0.202
4.871GlyIle: 4.871 ± 0.382
5.13GlyLys: 5.13 ± 0.33
4.71GlyLeu: 4.71 ± 0.414
1.936GlyMet: 1.936 ± 0.268
3.355GlyAsn: 3.355 ± 0.478
0.936GlyPro: 0.936 ± 0.169
2.194GlyGln: 2.194 ± 0.297
2.613GlyArg: 2.613 ± 0.292
3.968GlySer: 3.968 ± 0.372
3.387GlyThr: 3.387 ± 0.368
4.71GlyVal: 4.71 ± 0.359
1.032GlyTrp: 1.032 ± 0.209
2.839GlyTyr: 2.839 ± 0.31
0.0GlyXaa: 0.0 ± 0.0
His
1.129HisAla: 1.129 ± 0.187
0.355HisCys: 0.355 ± 0.112
1.452HisAsp: 1.452 ± 0.248
1.097HisGlu: 1.097 ± 0.213
0.774HisPhe: 0.774 ± 0.189
1.355HisGly: 1.355 ± 0.221
0.484HisHis: 0.484 ± 0.143
1.258HisIle: 1.258 ± 0.204
1.161HisLys: 1.161 ± 0.209
1.774HisLeu: 1.774 ± 0.227
0.419HisMet: 0.419 ± 0.126
1.065HisAsn: 1.065 ± 0.205
1.032HisPro: 1.032 ± 0.174
0.484HisGln: 0.484 ± 0.144
1.258HisArg: 1.258 ± 0.215
1.065HisSer: 1.065 ± 0.202
0.871HisThr: 0.871 ± 0.183
0.936HisVal: 0.936 ± 0.208
0.129HisTrp: 0.129 ± 0.073
0.742HisTyr: 0.742 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
5.162IleAla: 5.162 ± 0.396
1.065IleCys: 1.065 ± 0.197
4.871IleAsp: 4.871 ± 0.445
4.646IleGlu: 4.646 ± 0.371
2.226IlePhe: 2.226 ± 0.265
4.226IleGly: 4.226 ± 0.354
1.323IleHis: 1.323 ± 0.189
4.517IleIle: 4.517 ± 0.364
4.839IleLys: 4.839 ± 0.381
5.388IleLeu: 5.388 ± 0.432
1.774IleMet: 1.774 ± 0.272
3.968IleAsn: 3.968 ± 0.369
2.678IlePro: 2.678 ± 0.329
2.323IleGln: 2.323 ± 0.319
2.807IleArg: 2.807 ± 0.249
4.581IleSer: 4.581 ± 0.316
4.388IleThr: 4.388 ± 0.386
3.516IleVal: 3.516 ± 0.307
0.677IleTrp: 0.677 ± 0.164
2.387IleTyr: 2.387 ± 0.322
0.0IleXaa: 0.0 ± 0.0
Lys
6.226LysAla: 6.226 ± 0.572
0.613LysCys: 0.613 ± 0.156
4.678LysAsp: 4.678 ± 0.39
4.839LysGlu: 4.839 ± 0.38
3.549LysPhe: 3.549 ± 0.306
3.323LysGly: 3.323 ± 0.359
1.516LysHis: 1.516 ± 0.238
4.0LysIle: 4.0 ± 0.359
4.775LysLys: 4.775 ± 0.475
6.484LysLeu: 6.484 ± 0.498
2.387LysMet: 2.387 ± 0.274
3.646LysAsn: 3.646 ± 0.333
2.355LysPro: 2.355 ± 0.236
3.226LysGln: 3.226 ± 0.331
3.742LysArg: 3.742 ± 0.333
4.452LysSer: 4.452 ± 0.455
4.0LysThr: 4.0 ± 0.395
5.097LysVal: 5.097 ± 0.459
0.677LysTrp: 0.677 ± 0.162
3.162LysTyr: 3.162 ± 0.314
0.0LysXaa: 0.0 ± 0.0
Leu
7.13LeuAla: 7.13 ± 0.596
0.71LeuCys: 0.71 ± 0.154
6.226LeuAsp: 6.226 ± 0.469
6.678LeuGlu: 6.678 ± 0.475
2.71LeuPhe: 2.71 ± 0.369
5.226LeuGly: 5.226 ± 0.471
1.678LeuHis: 1.678 ± 0.252
5.549LeuIle: 5.549 ± 0.46
6.355LeuLys: 6.355 ± 0.492
6.517LeuLeu: 6.517 ± 0.537
2.194LeuMet: 2.194 ± 0.303
4.968LeuAsn: 4.968 ± 0.452
3.226LeuPro: 3.226 ± 0.322
3.258LeuGln: 3.258 ± 0.363
3.678LeuArg: 3.678 ± 0.315
4.71LeuSer: 4.71 ± 0.418
5.033LeuThr: 5.033 ± 0.476
5.097LeuVal: 5.097 ± 0.447
0.677LeuTrp: 0.677 ± 0.132
2.774LeuTyr: 2.774 ± 0.311
0.0LeuXaa: 0.0 ± 0.0
Met
1.613MetAla: 1.613 ± 0.243
0.226MetCys: 0.226 ± 0.083
1.161MetAsp: 1.161 ± 0.186
2.129MetGlu: 2.129 ± 0.276
0.968MetPhe: 0.968 ± 0.166
1.549MetGly: 1.549 ± 0.223
0.452MetHis: 0.452 ± 0.121
1.936MetIle: 1.936 ± 0.278
2.581MetLys: 2.581 ± 0.287
2.323MetLeu: 2.323 ± 0.323
0.548MetMet: 0.548 ± 0.15
1.129MetAsn: 1.129 ± 0.19
0.936MetPro: 0.936 ± 0.145
1.387MetGln: 1.387 ± 0.253
1.29MetArg: 1.29 ± 0.17
2.387MetSer: 2.387 ± 0.311
2.0MetThr: 2.0 ± 0.235
1.323MetVal: 1.323 ± 0.223
0.161MetTrp: 0.161 ± 0.061
0.903MetTyr: 0.903 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
3.904AsnAla: 3.904 ± 0.575
0.484AsnCys: 0.484 ± 0.139
2.678AsnAsp: 2.678 ± 0.296
2.807AsnGlu: 2.807 ± 0.314
2.194AsnPhe: 2.194 ± 0.278
4.129AsnGly: 4.129 ± 0.419
0.742AsnHis: 0.742 ± 0.147
3.904AsnIle: 3.904 ± 0.375
3.549AsnLys: 3.549 ± 0.362
4.355AsnLeu: 4.355 ± 0.39
1.258AsnMet: 1.258 ± 0.184
2.968AsnAsn: 2.968 ± 0.332
2.42AsnPro: 2.42 ± 0.28
1.839AsnGln: 1.839 ± 0.297
2.291AsnArg: 2.291 ± 0.275
3.71AsnSer: 3.71 ± 0.352
3.033AsnThr: 3.033 ± 0.295
3.484AsnVal: 3.484 ± 0.309
0.645AsnTrp: 0.645 ± 0.139
1.613AsnTyr: 1.613 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
2.387ProAla: 2.387 ± 0.333
0.419ProCys: 0.419 ± 0.113
2.549ProAsp: 2.549 ± 0.28
3.033ProGlu: 3.033 ± 0.348
1.419ProPhe: 1.419 ± 0.217
2.226ProGly: 2.226 ± 0.265
0.645ProHis: 0.645 ± 0.149
2.291ProIle: 2.291 ± 0.265
1.903ProLys: 1.903 ± 0.263
2.129ProLeu: 2.129 ± 0.223
0.387ProMet: 0.387 ± 0.106
2.129ProAsn: 2.129 ± 0.317
1.387ProPro: 1.387 ± 0.272
1.065ProGln: 1.065 ± 0.213
1.549ProArg: 1.549 ± 0.229
1.871ProSer: 1.871 ± 0.263
1.903ProThr: 1.903 ± 0.24
2.516ProVal: 2.516 ± 0.316
0.419ProTrp: 0.419 ± 0.107
1.678ProTyr: 1.678 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
3.226GlnAla: 3.226 ± 0.447
0.516GlnCys: 0.516 ± 0.122
2.065GlnAsp: 2.065 ± 0.314
2.71GlnGlu: 2.71 ± 0.359
1.645GlnPhe: 1.645 ± 0.232
1.871GlnGly: 1.871 ± 0.2
0.645GlnHis: 0.645 ± 0.142
2.42GlnIle: 2.42 ± 0.275
2.645GlnLys: 2.645 ± 0.385
4.162GlnLeu: 4.162 ± 0.425
1.161GlnMet: 1.161 ± 0.253
1.549GlnAsn: 1.549 ± 0.23
0.548GlnPro: 0.548 ± 0.142
2.774GlnGln: 2.774 ± 0.599
1.678GlnArg: 1.678 ± 0.256
2.323GlnSer: 2.323 ± 0.272
2.226GlnThr: 2.226 ± 0.271
2.871GlnVal: 2.871 ± 0.305
0.581GlnTrp: 0.581 ± 0.166
1.419GlnTyr: 1.419 ± 0.194
0.0GlnXaa: 0.0 ± 0.0
Arg
3.033ArgAla: 3.033 ± 0.321
0.355ArgCys: 0.355 ± 0.105
2.936ArgAsp: 2.936 ± 0.264
2.807ArgGlu: 2.807 ± 0.325
1.807ArgPhe: 1.807 ± 0.238
3.129ArgGly: 3.129 ± 0.316
0.613ArgHis: 0.613 ± 0.142
2.807ArgIle: 2.807 ± 0.399
3.097ArgLys: 3.097 ± 0.362
3.936ArgLeu: 3.936 ± 0.294
1.484ArgMet: 1.484 ± 0.243
2.678ArgAsn: 2.678 ± 0.287
1.29ArgPro: 1.29 ± 0.205
1.839ArgGln: 1.839 ± 0.247
2.323ArgArg: 2.323 ± 0.312
2.42ArgSer: 2.42 ± 0.247
2.645ArgThr: 2.645 ± 0.264
3.065ArgVal: 3.065 ± 0.32
0.419ArgTrp: 0.419 ± 0.116
1.678ArgTyr: 1.678 ± 0.234
0.0ArgXaa: 0.0 ± 0.0
Ser
4.517SerAla: 4.517 ± 0.761
0.677SerCys: 0.677 ± 0.15
3.226SerAsp: 3.226 ± 0.311
3.968SerGlu: 3.968 ± 0.689
3.226SerPhe: 3.226 ± 0.302
4.517SerGly: 4.517 ± 0.368
1.032SerHis: 1.032 ± 0.175
4.194SerIle: 4.194 ± 0.419
4.323SerLys: 4.323 ± 0.369
5.968SerLeu: 5.968 ± 0.43
1.71SerMet: 1.71 ± 0.247
3.323SerAsn: 3.323 ± 0.283
1.936SerPro: 1.936 ± 0.301
1.903SerGln: 1.903 ± 0.281
2.807SerArg: 2.807 ± 0.309
3.678SerSer: 3.678 ± 0.358
3.646SerThr: 3.646 ± 0.288
4.0SerVal: 4.0 ± 0.368
1.097SerTrp: 1.097 ± 0.168
2.549SerTyr: 2.549 ± 0.272
0.0SerXaa: 0.0 ± 0.0
Thr
4.452ThrAla: 4.452 ± 0.428
0.516ThrCys: 0.516 ± 0.135
2.936ThrAsp: 2.936 ± 0.313
3.516ThrGlu: 3.516 ± 0.333
2.484ThrPhe: 2.484 ± 0.263
4.678ThrGly: 4.678 ± 0.429
0.807ThrHis: 0.807 ± 0.155
4.129ThrIle: 4.129 ± 0.359
3.613ThrLys: 3.613 ± 0.38
4.613ThrLeu: 4.613 ± 0.396
1.387ThrMet: 1.387 ± 0.209
3.194ThrAsn: 3.194 ± 0.333
2.355ThrPro: 2.355 ± 0.256
2.452ThrGln: 2.452 ± 0.441
2.258ThrArg: 2.258 ± 0.312
3.839ThrSer: 3.839 ± 0.425
3.033ThrThr: 3.033 ± 0.283
4.452ThrVal: 4.452 ± 0.379
0.613ThrTrp: 0.613 ± 0.132
2.323ThrTyr: 2.323 ± 0.315
0.0ThrXaa: 0.0 ± 0.0
Val
5.291ValAla: 5.291 ± 0.405
0.613ValCys: 0.613 ± 0.148
4.291ValAsp: 4.291 ± 0.437
4.517ValGlu: 4.517 ± 0.374
2.645ValPhe: 2.645 ± 0.266
3.516ValGly: 3.516 ± 0.329
1.129ValHis: 1.129 ± 0.166
4.71ValIle: 4.71 ± 0.372
5.065ValLys: 5.065 ± 0.353
4.581ValLeu: 4.581 ± 0.298
1.549ValMet: 1.549 ± 0.199
3.129ValAsn: 3.129 ± 0.396
2.678ValPro: 2.678 ± 0.355
2.258ValGln: 2.258 ± 0.271
2.549ValArg: 2.549 ± 0.292
4.484ValSer: 4.484 ± 0.38
3.871ValThr: 3.871 ± 0.351
4.291ValVal: 4.291 ± 0.493
0.71ValTrp: 0.71 ± 0.157
2.581ValTyr: 2.581 ± 0.282
0.0ValXaa: 0.0 ± 0.0
Trp
0.548TrpAla: 0.548 ± 0.115
0.29TrpCys: 0.29 ± 0.1
1.0TrpAsp: 1.0 ± 0.165
1.194TrpGlu: 1.194 ± 0.213
0.548TrpPhe: 0.548 ± 0.141
0.742TrpGly: 0.742 ± 0.213
0.226TrpHis: 0.226 ± 0.08
0.613TrpIle: 0.613 ± 0.148
0.968TrpLys: 0.968 ± 0.148
1.323TrpLeu: 1.323 ± 0.204
0.29TrpMet: 0.29 ± 0.091
0.516TrpAsn: 0.516 ± 0.127
0.258TrpPro: 0.258 ± 0.105
0.548TrpGln: 0.548 ± 0.145
0.516TrpArg: 0.516 ± 0.121
0.774TrpSer: 0.774 ± 0.181
0.613TrpThr: 0.613 ± 0.157
0.677TrpVal: 0.677 ± 0.142
0.194TrpTrp: 0.194 ± 0.098
0.355TrpTyr: 0.355 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.387TyrAla: 2.387 ± 0.275
0.677TyrCys: 0.677 ± 0.137
3.0TyrAsp: 3.0 ± 0.349
2.097TyrGlu: 2.097 ± 0.271
1.742TyrPhe: 1.742 ± 0.274
2.355TyrGly: 2.355 ± 0.297
1.258TyrHis: 1.258 ± 0.176
2.258TyrIle: 2.258 ± 0.32
2.678TyrLys: 2.678 ± 0.299
2.807TyrLeu: 2.807 ± 0.281
0.936TyrMet: 0.936 ± 0.162
2.484TyrAsn: 2.484 ± 0.311
1.258TyrPro: 1.258 ± 0.159
1.742TyrGln: 1.742 ± 0.223
1.839TyrArg: 1.839 ± 0.237
2.258TyrSer: 2.258 ± 0.26
2.452TyrThr: 2.452 ± 0.263
2.387TyrVal: 2.387 ± 0.293
0.581TyrTrp: 0.581 ± 0.125
1.516TyrTyr: 1.516 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 166 proteins (30998 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski