Amino acid dipepetide frequency for Gordonia phage Lozinak

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.425AlaAla: 10.425 ± 0.845
0.579AlaCys: 0.579 ± 0.171
6.814AlaAsp: 6.814 ± 0.46
6.541AlaGlu: 6.541 ± 0.542
2.93AlaPhe: 2.93 ± 0.449
7.495AlaGly: 7.495 ± 0.533
2.18AlaHis: 2.18 ± 0.358
4.599AlaIle: 4.599 ± 0.401
3.952AlaLys: 3.952 ± 0.517
7.836AlaLeu: 7.836 ± 0.563
2.623AlaMet: 2.623 ± 0.279
3.305AlaAsn: 3.305 ± 0.36
4.327AlaPro: 4.327 ± 0.471
4.701AlaGln: 4.701 ± 0.662
6.507AlaArg: 6.507 ± 0.576
4.906AlaSer: 4.906 ± 0.389
4.838AlaThr: 4.838 ± 0.45
7.086AlaVal: 7.086 ± 0.558
1.84AlaTrp: 1.84 ± 0.25
2.283AlaTyr: 2.283 ± 0.261
0.0AlaXaa: 0.0 ± 0.0
Cys
0.681CysAla: 0.681 ± 0.16
0.204CysCys: 0.204 ± 0.093
1.022CysAsp: 1.022 ± 0.253
0.784CysGlu: 0.784 ± 0.172
0.307CysPhe: 0.307 ± 0.106
1.192CysGly: 1.192 ± 0.273
0.375CysHis: 0.375 ± 0.14
0.409CysIle: 0.409 ± 0.107
0.545CysLys: 0.545 ± 0.142
0.511CysLeu: 0.511 ± 0.144
0.034CysMet: 0.034 ± 0.036
0.409CysAsn: 0.409 ± 0.134
0.579CysPro: 0.579 ± 0.156
0.477CysGln: 0.477 ± 0.129
0.749CysArg: 0.749 ± 0.17
0.443CysSer: 0.443 ± 0.115
0.477CysThr: 0.477 ± 0.145
0.852CysVal: 0.852 ± 0.177
0.17CysTrp: 0.17 ± 0.071
0.136CysTyr: 0.136 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
6.303AspAla: 6.303 ± 0.489
0.715AspCys: 0.715 ± 0.19
4.838AspAsp: 4.838 ± 0.481
5.076AspGlu: 5.076 ± 0.514
2.453AspPhe: 2.453 ± 0.28
5.826AspGly: 5.826 ± 0.462
1.669AspHis: 1.669 ± 0.197
3.441AspIle: 3.441 ± 0.42
2.623AspLys: 2.623 ± 0.318
5.962AspLeu: 5.962 ± 0.466
1.806AspMet: 1.806 ± 0.293
2.283AspAsn: 2.283 ± 0.36
4.293AspPro: 4.293 ± 0.408
2.112AspGln: 2.112 ± 0.345
5.417AspArg: 5.417 ± 0.546
2.93AspSer: 2.93 ± 0.307
4.293AspThr: 4.293 ± 0.434
5.315AspVal: 5.315 ± 0.391
1.261AspTrp: 1.261 ± 0.237
2.76AspTyr: 2.76 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
7.018GluAla: 7.018 ± 0.774
0.681GluCys: 0.681 ± 0.188
4.156GluAsp: 4.156 ± 0.411
4.19GluGlu: 4.19 ± 0.377
2.385GluPhe: 2.385 ± 0.319
4.77GluGly: 4.77 ± 0.333
1.329GluHis: 1.329 ± 0.256
4.054GluIle: 4.054 ± 0.401
2.589GluLys: 2.589 ± 0.347
6.371GluLeu: 6.371 ± 0.511
2.146GluMet: 2.146 ± 0.238
2.146GluAsn: 2.146 ± 0.258
2.896GluPro: 2.896 ± 0.514
2.589GluGln: 2.589 ± 0.306
4.463GluArg: 4.463 ± 0.408
3.168GluSer: 3.168 ± 0.307
3.645GluThr: 3.645 ± 0.377
4.565GluVal: 4.565 ± 0.436
2.078GluTrp: 2.078 ± 0.279
2.283GluTyr: 2.283 ± 0.28
0.0GluXaa: 0.0 ± 0.0
Phe
3.168PheAla: 3.168 ± 0.293
0.443PheCys: 0.443 ± 0.123
2.896PheAsp: 2.896 ± 0.309
2.385PheGlu: 2.385 ± 0.302
0.647PhePhe: 0.647 ± 0.149
2.214PheGly: 2.214 ± 0.309
0.749PheHis: 0.749 ± 0.128
1.397PheIle: 1.397 ± 0.22
1.226PheLys: 1.226 ± 0.173
2.112PheLeu: 2.112 ± 0.278
1.022PheMet: 1.022 ± 0.21
1.567PheAsn: 1.567 ± 0.226
1.363PhePro: 1.363 ± 0.222
0.749PheGln: 0.749 ± 0.141
1.499PheArg: 1.499 ± 0.221
2.044PheSer: 2.044 ± 0.231
2.214PheThr: 2.214 ± 0.297
2.283PheVal: 2.283 ± 0.424
0.784PheTrp: 0.784 ± 0.171
0.818PheTyr: 0.818 ± 0.152
0.0PheXaa: 0.0 ± 0.0
Gly
6.03GlyAla: 6.03 ± 0.625
0.988GlyCys: 0.988 ± 0.234
5.212GlyAsp: 5.212 ± 0.51
5.008GlyGlu: 5.008 ± 0.428
2.725GlyPhe: 2.725 ± 0.33
6.2GlyGly: 6.2 ± 0.788
1.942GlyHis: 1.942 ± 0.259
3.952GlyIle: 3.952 ± 0.338
3.611GlyLys: 3.611 ± 0.367
5.894GlyLeu: 5.894 ± 0.513
2.146GlyMet: 2.146 ± 0.237
2.76GlyAsn: 2.76 ± 0.352
3.747GlyPro: 3.747 ± 0.37
3.202GlyGln: 3.202 ± 0.272
4.77GlyArg: 4.77 ± 0.321
4.497GlySer: 4.497 ± 0.352
4.701GlyThr: 4.701 ± 0.517
6.439GlyVal: 6.439 ± 0.459
2.214GlyTrp: 2.214 ± 0.311
2.521GlyTyr: 2.521 ± 0.288
0.0GlyXaa: 0.0 ± 0.0
His
1.703HisAla: 1.703 ± 0.267
0.273HisCys: 0.273 ± 0.123
1.192HisAsp: 1.192 ± 0.24
1.567HisGlu: 1.567 ± 0.221
1.056HisPhe: 1.056 ± 0.203
2.078HisGly: 2.078 ± 0.323
0.477HisHis: 0.477 ± 0.154
1.056HisIle: 1.056 ± 0.205
0.647HisLys: 0.647 ± 0.124
2.112HisLeu: 2.112 ± 0.324
0.443HisMet: 0.443 ± 0.13
0.784HisAsn: 0.784 ± 0.179
1.397HisPro: 1.397 ± 0.211
0.784HisGln: 0.784 ± 0.179
1.84HisArg: 1.84 ± 0.265
1.431HisSer: 1.431 ± 0.213
1.363HisThr: 1.363 ± 0.2
1.806HisVal: 1.806 ± 0.258
0.443HisTrp: 0.443 ± 0.11
0.954HisTyr: 0.954 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
4.633IleAla: 4.633 ± 0.36
0.579IleCys: 0.579 ± 0.142
4.054IleAsp: 4.054 ± 0.376
3.543IleGlu: 3.543 ± 0.367
1.056IlePhe: 1.056 ± 0.165
3.134IleGly: 3.134 ± 0.307
1.09IleHis: 1.09 ± 0.173
2.078IleIle: 2.078 ± 0.365
2.18IleLys: 2.18 ± 0.304
3.168IleLeu: 3.168 ± 0.396
0.545IleMet: 0.545 ± 0.119
1.737IleAsn: 1.737 ± 0.316
2.453IlePro: 2.453 ± 0.293
1.806IleGln: 1.806 ± 0.245
3.918IleArg: 3.918 ± 0.372
2.896IleSer: 2.896 ± 0.393
3.134IleThr: 3.134 ± 0.302
3.1IleVal: 3.1 ± 0.369
0.954IleTrp: 0.954 ± 0.181
1.295IleTyr: 1.295 ± 0.199
0.0IleXaa: 0.0 ± 0.0
Lys
3.339LysAla: 3.339 ± 0.361
0.613LysCys: 0.613 ± 0.18
2.521LysAsp: 2.521 ± 0.38
2.146LysGlu: 2.146 ± 0.295
1.567LysPhe: 1.567 ± 0.273
3.134LysGly: 3.134 ± 0.395
0.886LysHis: 0.886 ± 0.186
1.635LysIle: 1.635 ± 0.211
2.214LysLys: 2.214 ± 0.317
3.816LysLeu: 3.816 ± 0.368
1.158LysMet: 1.158 ± 0.231
1.261LysAsn: 1.261 ± 0.229
2.419LysPro: 2.419 ± 0.304
1.124LysGln: 1.124 ± 0.235
2.998LysArg: 2.998 ± 0.321
2.555LysSer: 2.555 ± 0.31
1.84LysThr: 1.84 ± 0.213
3.747LysVal: 3.747 ± 0.389
0.749LysTrp: 0.749 ± 0.167
1.124LysTyr: 1.124 ± 0.162
0.0LysXaa: 0.0 ± 0.0
Leu
7.052LeuAla: 7.052 ± 0.618
1.022LeuCys: 1.022 ± 0.257
6.234LeuAsp: 6.234 ± 0.436
5.962LeuGlu: 5.962 ± 0.518
2.18LeuPhe: 2.18 ± 0.323
5.962LeuGly: 5.962 ± 0.512
1.806LeuHis: 1.806 ± 0.258
3.509LeuIle: 3.509 ± 0.304
2.657LeuLys: 2.657 ± 0.327
5.144LeuLeu: 5.144 ± 0.459
1.567LeuMet: 1.567 ± 0.262
3.475LeuAsn: 3.475 ± 0.349
3.918LeuPro: 3.918 ± 0.402
3.168LeuGln: 3.168 ± 0.353
5.349LeuArg: 5.349 ± 0.425
4.156LeuSer: 4.156 ± 0.297
4.838LeuThr: 4.838 ± 0.454
5.042LeuVal: 5.042 ± 0.48
1.192LeuTrp: 1.192 ± 0.197
2.01LeuTyr: 2.01 ± 0.226
0.0LeuXaa: 0.0 ± 0.0
Met
2.896MetAla: 2.896 ± 0.321
0.136MetCys: 0.136 ± 0.065
1.431MetAsp: 1.431 ± 0.228
1.192MetGlu: 1.192 ± 0.198
0.715MetPhe: 0.715 ± 0.166
1.465MetGly: 1.465 ± 0.186
0.647MetHis: 0.647 ± 0.166
1.124MetIle: 1.124 ± 0.197
0.784MetLys: 0.784 ± 0.143
1.635MetLeu: 1.635 ± 0.213
0.341MetMet: 0.341 ± 0.105
1.158MetAsn: 1.158 ± 0.187
1.499MetPro: 1.499 ± 0.198
0.954MetGln: 0.954 ± 0.187
1.874MetArg: 1.874 ± 0.272
2.623MetSer: 2.623 ± 0.295
2.248MetThr: 2.248 ± 0.309
1.533MetVal: 1.533 ± 0.237
0.307MetTrp: 0.307 ± 0.102
0.443MetTyr: 0.443 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
3.679AsnAla: 3.679 ± 0.453
0.613AsnCys: 0.613 ± 0.148
2.351AsnAsp: 2.351 ± 0.241
2.521AsnGlu: 2.521 ± 0.223
0.886AsnPhe: 0.886 ± 0.163
3.679AsnGly: 3.679 ± 0.37
0.886AsnHis: 0.886 ± 0.193
1.499AsnIle: 1.499 ± 0.247
1.465AsnLys: 1.465 ± 0.287
3.168AsnLeu: 3.168 ± 0.313
0.511AsnMet: 0.511 ± 0.159
1.226AsnAsn: 1.226 ± 0.233
2.146AsnPro: 2.146 ± 0.258
1.397AsnGln: 1.397 ± 0.226
2.351AsnArg: 2.351 ± 0.31
2.078AsnSer: 2.078 ± 0.256
2.078AsnThr: 2.078 ± 0.371
2.351AsnVal: 2.351 ± 0.3
0.579AsnTrp: 0.579 ± 0.143
1.09AsnTyr: 1.09 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
5.417ProAla: 5.417 ± 0.48
0.341ProCys: 0.341 ± 0.109
3.747ProAsp: 3.747 ± 0.369
3.713ProGlu: 3.713 ± 0.484
1.806ProPhe: 1.806 ± 0.296
4.633ProGly: 4.633 ± 0.421
0.818ProHis: 0.818 ± 0.178
2.248ProIle: 2.248 ± 0.265
2.18ProLys: 2.18 ± 0.312
2.896ProLeu: 2.896 ± 0.33
0.954ProMet: 0.954 ± 0.197
1.601ProAsn: 1.601 ± 0.194
2.044ProPro: 2.044 ± 0.246
1.669ProGln: 1.669 ± 0.261
2.725ProArg: 2.725 ± 0.335
2.862ProSer: 2.862 ± 0.383
3.066ProThr: 3.066 ± 0.494
3.441ProVal: 3.441 ± 0.332
1.124ProTrp: 1.124 ± 0.202
1.567ProTyr: 1.567 ± 0.219
0.0ProXaa: 0.0 ± 0.0
Gln
3.475GlnAla: 3.475 ± 0.466
0.341GlnCys: 0.341 ± 0.092
1.942GlnAsp: 1.942 ± 0.275
2.078GlnGlu: 2.078 ± 0.329
1.022GlnPhe: 1.022 ± 0.187
2.623GlnGly: 2.623 ± 0.26
0.886GlnHis: 0.886 ± 0.199
2.01GlnIle: 2.01 ± 0.219
1.669GlnLys: 1.669 ± 0.357
3.747GlnLeu: 3.747 ± 0.412
1.261GlnMet: 1.261 ± 0.192
1.703GlnAsn: 1.703 ± 0.304
1.397GlnPro: 1.397 ± 0.237
1.669GlnGln: 1.669 ± 0.371
2.623GlnArg: 2.623 ± 0.385
1.976GlnSer: 1.976 ± 0.369
1.874GlnThr: 1.874 ± 0.3
2.419GlnVal: 2.419 ± 0.259
0.92GlnTrp: 0.92 ± 0.156
0.92GlnTyr: 0.92 ± 0.196
0.0GlnXaa: 0.0 ± 0.0
Arg
6.098ArgAla: 6.098 ± 0.425
0.715ArgCys: 0.715 ± 0.189
4.804ArgAsp: 4.804 ± 0.346
5.008ArgGlu: 5.008 ± 0.376
2.18ArgPhe: 2.18 ± 0.28
4.667ArgGly: 4.667 ± 0.407
1.942ArgHis: 1.942 ± 0.283
3.134ArgIle: 3.134 ± 0.284
3.271ArgLys: 3.271 ± 0.323
5.383ArgLeu: 5.383 ± 0.419
1.533ArgMet: 1.533 ± 0.242
2.623ArgAsn: 2.623 ± 0.242
2.657ArgPro: 2.657 ± 0.316
2.589ArgGln: 2.589 ± 0.278
5.962ArgArg: 5.962 ± 0.447
4.259ArgSer: 4.259 ± 0.345
3.577ArgThr: 3.577 ± 0.356
5.519ArgVal: 5.519 ± 0.466
1.635ArgTrp: 1.635 ± 0.265
2.283ArgTyr: 2.283 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
5.928SerAla: 5.928 ± 0.447
0.443SerCys: 0.443 ± 0.14
4.054SerAsp: 4.054 ± 0.37
3.543SerGlu: 3.543 ± 0.317
1.806SerPhe: 1.806 ± 0.223
5.008SerGly: 5.008 ± 0.474
1.295SerHis: 1.295 ± 0.242
2.76SerIle: 2.76 ± 0.267
2.18SerLys: 2.18 ± 0.283
4.02SerLeu: 4.02 ± 0.32
1.976SerMet: 1.976 ± 0.293
1.942SerAsn: 1.942 ± 0.334
2.453SerPro: 2.453 ± 0.29
1.601SerGln: 1.601 ± 0.254
4.054SerArg: 4.054 ± 0.317
3.168SerSer: 3.168 ± 0.289
3.236SerThr: 3.236 ± 0.351
4.531SerVal: 4.531 ± 0.417
1.465SerTrp: 1.465 ± 0.211
1.84SerTyr: 1.84 ± 0.254
0.0SerXaa: 0.0 ± 0.0
Thr
6.064ThrAla: 6.064 ± 0.457
0.579ThrCys: 0.579 ± 0.153
4.259ThrAsp: 4.259 ± 0.341
4.156ThrGlu: 4.156 ± 0.277
1.908ThrPhe: 1.908 ± 0.21
4.735ThrGly: 4.735 ± 0.398
1.192ThrHis: 1.192 ± 0.202
2.828ThrIle: 2.828 ± 0.274
2.691ThrLys: 2.691 ± 0.371
3.986ThrLeu: 3.986 ± 0.327
1.363ThrMet: 1.363 ± 0.209
1.874ThrAsn: 1.874 ± 0.227
4.156ThrPro: 4.156 ± 0.494
1.806ThrGln: 1.806 ± 0.289
3.577ThrArg: 3.577 ± 0.352
3.679ThrSer: 3.679 ± 0.451
2.896ThrThr: 2.896 ± 0.339
4.054ThrVal: 4.054 ± 0.425
1.09ThrTrp: 1.09 ± 0.18
1.703ThrTyr: 1.703 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
7.427ValAla: 7.427 ± 0.582
0.579ValCys: 0.579 ± 0.154
5.996ValAsp: 5.996 ± 0.417
4.872ValGlu: 4.872 ± 0.501
2.487ValPhe: 2.487 ± 0.321
5.894ValGly: 5.894 ± 0.395
1.737ValHis: 1.737 ± 0.275
3.475ValIle: 3.475 ± 0.383
2.623ValLys: 2.623 ± 0.281
4.872ValLeu: 4.872 ± 0.386
2.146ValMet: 2.146 ± 0.259
2.385ValAsn: 2.385 ± 0.294
3.168ValPro: 3.168 ± 0.381
2.214ValGln: 2.214 ± 0.27
5.281ValArg: 5.281 ± 0.434
4.531ValSer: 4.531 ± 0.432
4.974ValThr: 4.974 ± 0.497
5.723ValVal: 5.723 ± 0.544
1.397ValTrp: 1.397 ± 0.201
1.976ValTyr: 1.976 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
1.669TrpAla: 1.669 ± 0.237
0.273TrpCys: 0.273 ± 0.108
1.567TrpAsp: 1.567 ± 0.215
1.533TrpGlu: 1.533 ± 0.226
0.647TrpPhe: 0.647 ± 0.163
1.465TrpGly: 1.465 ± 0.248
0.784TrpHis: 0.784 ± 0.171
1.09TrpIle: 1.09 ± 0.189
0.715TrpLys: 0.715 ± 0.144
1.635TrpLeu: 1.635 ± 0.264
0.784TrpMet: 0.784 ± 0.147
0.92TrpAsn: 0.92 ± 0.183
0.886TrpPro: 0.886 ± 0.184
0.818TrpGln: 0.818 ± 0.173
1.261TrpArg: 1.261 ± 0.229
1.329TrpSer: 1.329 ± 0.239
1.635TrpThr: 1.635 ± 0.292
1.397TrpVal: 1.397 ± 0.21
0.511TrpTrp: 0.511 ± 0.132
0.545TrpTyr: 0.545 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.76TyrAla: 2.76 ± 0.28
0.238TyrCys: 0.238 ± 0.1
2.283TyrAsp: 2.283 ± 0.337
1.703TyrGlu: 1.703 ± 0.277
0.818TyrPhe: 0.818 ± 0.187
2.385TyrGly: 2.385 ± 0.265
0.715TyrHis: 0.715 ± 0.165
1.056TyrIle: 1.056 ± 0.205
1.022TyrLys: 1.022 ± 0.194
1.976TyrLeu: 1.976 ± 0.235
0.613TyrMet: 0.613 ± 0.147
1.397TyrAsn: 1.397 ± 0.245
1.09TyrPro: 1.09 ± 0.246
1.124TyrGln: 1.124 ± 0.203
2.623TyrArg: 2.623 ± 0.309
1.772TyrSer: 1.772 ± 0.262
1.669TyrThr: 1.669 ± 0.24
2.555TyrVal: 2.555 ± 0.333
0.749TyrTrp: 0.749 ± 0.162
0.511TyrTyr: 0.511 ± 0.127
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 179 proteins (29354 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski